Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,980,272 G→T 12.7% noncoding (529/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,980,2720GT12.7% 4.0 / 10.4 53noncoding (529/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (3/43);  new base T (2/5);  total (5/48)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATC  >  NZ_CP034943/2980146‑2980390
                                                                                                                                 |                                                                                                                      
gcgtAACCGTGCACGGGATCGTAACTCGCCGGTTCAGTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTc                                                                                                           <  2:5435919/137‑1 (MQ=0)
 ctgaaCCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTcc                                                                                                          <  1:4517959/137‑1 (MQ=1)
   gaaccgggCACGGGCTCGCACCCCGCCGCTTGAGTCTACAAAACGCCCGCCATCCCCCGTTAACGGGCTCGGACTCCTGGTATGCACACGGGTTCAGGATCTCTTGCACTCCCCTTCCGGGGTGCTTTTCACCGGTCCCt                                                                                                        <  2:2941715/133‑1 (MQ=0)
      ccgtGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAc                                                                                                     <  2:2113600/137‑1 (MQ=1)
      ccgtGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGGTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAc                                                                                                     <  1:1576572/137‑1 (MQ=1)
      ccctGCACGGGATCGTAACTCTCCGGTTCATTCTAGAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAc                                                                                                     <  2:2280523/137‑1 (MQ=0)
         tgaaCGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGt                                                                                                  >  2:3347368/4‑140 (MQ=1)
           ccccggctCGTACCTCGCCGCTCCCTCCCACCCACGGCACGCCAGCACCCCCTAACGGGCGCGGACTACTGGTAGGCACACGGGGTCCGGATCTCTTTCACTCCCCGTCCGGGGTGCTTGTCACCTTTCCCTCACGGTAc                                                                                                <  2:535284/133‑1 (MQ=0)
           catgggATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTGGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTGTCCCTCACGGTAc                                                                                                <  2:2461933/137‑1 (MQ=0)
                       acgcGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATc                                                                                    <  1:812291/137‑1 (MQ=1)
                          cggcGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGt                                                                                 <  1:2937219/137‑1 (MQ=1)
                                  cagtCTACAAAAGGCACGCCATCACGCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCTCTAggg                                                                         <  2:3360999/137‑1 (MQ=0)
                                  cagtCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAggg                                                                         <  2:5527893/137‑1 (MQ=1)
                                    ttatACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAgg                                                                          <  2:1151961/137‑1 (MQ=0)
                                    ttatACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAgg                                                                          <  2:2485120/137‑1 (MQ=0)
                                    ttatACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAgg                                                                          <  2:432902/137‑1 (MQ=0)
                                    ttatACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAgg                                                                          <  2:2181517/137‑1 (MQ=0)
                                        agtaatggCAAGACATCACCCCTGAACGGGCTCGGTCTACTTGTAGGCACACGCTTTCAGGATCTCTTTCACTCGCCTTCCGTGTTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCCGTCACTAGGGAGTAtt                                                                   <  2:4526634/134‑1 (MQ=1)
                                         ccacagccACGCCATCACCCGTTAACGGGCTCTGACTACGTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCCCTAGGGAGTAttt                                                                  <  1:338107/133‑1 (MQ=0)
                                         cacaaGGCACGCCATCACCCGTTACCGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCGTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTAttt                                                                  <  1:5092325/137‑1 (MQ=0)
                                         cacaaGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTAttt                                                                  <  1:5232586/137‑1 (MQ=1)
                                         cacaaGGCACGCCATCACCCGGTCACGGGCTCTGACTACCGGTAGGCACACGGGGTCAGGATCGCTTTCCCTCCCCTGCCGGGGTGCTTGTCACCTTTCCCTCCCGGTACTGGGTCACTATCGGTCACTAGGGAGTAttt                                                                  <  2:5638803/137‑1 (MQ=1)
                                          aacaggcaggcCATCACCCGTTAACGGGCTCTGACTAATTGTATGCACACGTTTTCATGATCTCTTTCACTGCACTTCCGGGTTGCTTTTCTCCTTTCACTCAATGTACTGGTTCACTATCGGTCATTTTGGAGTAtttt                                                                 <  2:2077190/137‑2 (MQ=1)
                                             agtcACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGcc                                                              <  1:262488/137‑1 (MQ=1)
                                               gcccGCCATCACCCGTGAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTGTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCtt                                                            <  2:4451644/137‑1 (MQ=0)
                                                           cccgggaaCGGGCCCTGACTACACGTAGGCACACGGCCTCAGCAACTCCTCCCCTCCCCGACCGCGCTGCAATTCACCTGACCCCCCCCGTACCGGTTCACTATCGGTCACTAGGGAGTATTAAGCCTAGCGAGATGGtc                                                 <  2:4266759/134‑1 (MQ=0)
                                                                           tgtctacgTGTAGGCACACGTTTTCATTATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGAc                                  <  1:4889940/137‑1 (MQ=1)
                                                                              ctgctTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAcag                                >  1:793273/4‑138 (MQ=0)
                                                                              ctgctTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAcag                                >  1:3687113/4‑138 (MQ=0)
                                                                                        gaactcGGTTTCAGGATCTCTTTCACTGCCCTTCCGTGGTGCTTTTGAGCTTTCCCTCCCGGTACTTGTTAACTATCGGTCCGTAGGGAGTATTTTGCCTTGGGTGATGTTCCTCACGTATTCAGACGGAATTTCACgtg                     <  1:2671576/135‑1 (MQ=1)
                                                                                             cgctttCAGGATCTCTTTCACTCCCCTTCCGTGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTccg                <  1:5277467/137‑1 (MQ=0)
                                                                                               gtgtCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGGGTTccgcc              <  2:1050339/137‑1 (MQ=1)
                                                                                               gtgtCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTGCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTccgcc              <  1:4814224/137‑1 (MQ=1)
                                                                                                        tcactatCACTCCCCTTAAGGGCTGCTTTTCACATATCCCTCACGGAAATGGTACACTATAGGACACTCGGGAGTATTTAGACTTGGGAGATGGTACTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAgc     >  2:2054883/7‑139 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCCCGGTACTGGTTCACGATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGTACTCCCGGATTCCGACGGAATTTCAAGTGTTCCGCCGTACTCAGGAt   <  1:3150491/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTTCTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGTATTTCCCGTGTTCCGCCGTACTCAGGAt   <  1:2435142/137‑1 (MQ=0)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTCCTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  2:2638311/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  2:2834512/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  2:3405327/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  2:4723850/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  1:5081934/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  1:4998985/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTGCCGGGGTGCTTTTCACCTGGCCCACCCGGGACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  1:5548619/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCGTCCGGGGTGCGTTTCCCCTGTCCCGCCCGGTACTGGGTCACTCTCGGTCACGAGGGAGTATTTCGCCGTGGGAGATGGGCCGCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGa  <  2:861735/137‑2 (MQ=1)
                                                                                                          tcgttCACGCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTGAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  1:3611859/137‑1 (MQ=0)
                                                                                                          tccttCACTCCCCTTCCGGGGTGCTTGTCACCTTTCCCTCCCGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGAGTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  1:1603150/137‑1 (MQ=0)
                                                                                                          tcattCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCCCGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGACTTACACGTGTGACGCCGGACTCAGGa  >  2:3386150/4‑139 (MQ=1)
                                                                                                          tcattCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  2:3250385/137‑1 (MQ=1)
                                                                                                          gctgtcccTCCCCGTCCGGGGCGCTTTCCACCTGGCCCTCACGGTACTCGGTCACTAGCGGGCACTAGGGAGTATTTAGCCGTGGGAGATGGTCCTCCCGGATTCCGACGGAATTACACGTGTTCCGCCGTACTCAGGAt   <  2:5509059/133‑1 (MQ=1)
                                                                                                          gctgtCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAGTGGGTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  1:4789433/136‑1 (MQ=0)
                                                                                                          gctgtCACGCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTGAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  1:1168960/136‑1 (MQ=0)
                                                                                                          ccttgcccTCCCCTGCCGGGGTGCGTTTCACCTTGCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt   <  1:3318331/133‑1 (MQ=1)
                                                                                                           ctgtCACGCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATc  <  1:5789845/137‑1 (MQ=1)
                                                                                                                                 |                                                                                                                      
GCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATC  >  NZ_CP034943/2980146‑2980390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: