BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,341	C→A	9.8%	E127* (GAG→TAG)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,341	0	C	A	9.8%	847.3 / 26.3	337	E127* (GAG→TAG)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base C (215/79); new base A (2/41); total (217/120)
Fisher's exact test for biased strand distribution p-value = 1.09e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

CTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAA  >  NZ_CP034943/3280202‑3280480

cTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCTGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTc                                                                                                                                             <  2:3161601/140‑1 (MQ=255)

         tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTATTATAACAAGCGGAATTTAATGTAATAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAac                                                                                                                                    >  1:3170556/1‑140 (MQ=255)

         tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAac                                                                                                                                    >  1:2066763/1‑140 (MQ=255)

             aTTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATAGAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGa                                                                                                                                >  2:322330/1‑140 (MQ=255)

               tttCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGc                                                                                                                              >  2:3594649/1‑140 (MQ=255)

               tttCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAAGTTTCTAATTAATTTGTAGAGCCACTCAAAATCAACGCGAGc                                                                                                                              >  2:4445140/1‑140 (MQ=255)

                       tgtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATAGAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACCCGAGCCTTTTTGt                                                                                                                      <  1:2343020/140‑1 (MQ=255)

                              tACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAAGTAATTGGTAGAGCCACTCAAAATCAACACGAGCCGTTTTGTAAaatga                                                                                                               >  1:1152046/1‑140 (MQ=255)

                                   caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTGTAATTAATTGGTAGAGCCCCTCAAAATCAACACGGTCCTTTTTGTAAAATGAGTGtt                                                                                                          >  1:3265672/1‑140 (MQ=255)

                                   caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTGTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                          >  2:5698226/1‑140 (MQ=255)

                                   caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                          >  2:4724501/1‑140 (MQ=255)

                                   caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                          >  2:3766304/1‑140 (MQ=255)

                                   caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                          >  2:3536617/1‑140 (MQ=255)

                                   caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                          >  1:1193881/1‑140 (MQ=255)

                                      gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                       <  1:5145590/140‑1 (MQ=255)

                                      gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                       <  1:5801966/140‑1 (MQ=255)

                                      gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                       <  2:5167141/140‑1 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCACCACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  2:4022621/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGACTGTTGATTa                                                                                                     >  2:2429358/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  2:2389398/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  2:2252545/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  2:3696783/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  2:5430183/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  2:4348388/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  1:2474821/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  1:2395728/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                     >  1:2251345/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTCGATTa                                                                                                     >  2:4052570/1‑140 (MQ=255)

                                        aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAAGTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGACTGTTGATTa                                                                                                     >  2:535766/1‑140 (MQ=255)

                                           cccGGTCACCCTTAATATAACAATCGGAAGTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAAGTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTCAAATGAATGTTGATTaaat                                                                                                  >  1:3692828/1‑139 (MQ=255)

                                           cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTcaaa                                                                                                  >  1:5354444/1‑140 (MQ=255)

                                           cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                  >  1:3867768/1‑140 (MQ=255)

                                           cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                  >  1:5748392/1‑140 (MQ=255)

                                              ggTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAt                                                                                               >  2:3394625/1‑140 (MQ=255)

                                               ttCAACCTTAAAATAACAAGCGTAATTTAATGAAAAAGAAACTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                              >  1:2116677/2‑140 (MQ=255)

                                               gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                              >  2:2977948/1‑140 (MQ=255)

                                               gTCACCCTTAATATAACAAGCGGAATTTAAGGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATGTTCTATTTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                              >  1:832664/1‑140 (MQ=255)

                                                 cACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAg                                                                                            >  1:4647381/1‑140 (MQ=255)

                                                 cACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAg                                                                                            <  2:3655761/140‑1 (MQ=255)

                                                    ccTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGa                                                                                         >  2:1993136/1‑140 (MQ=255)

                                                          tataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTa                                                                                   >  1:1657286/1‑140 (MQ=255)

                                                              accaGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:2552559/4‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAATTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTTTTATAGCAAATAAAAATCAAAACGATCCTTTTTTTAAAATGAATGATGATTAAAAGATAAGTGAATTTTATAAg                                                                               >  1:3313547/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:491209/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:1824015/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:2159711/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:2849989/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:933946/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  1:3510787/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:141787/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  1:3011247/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  1:2720978/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAGAAGTGATTTTTATCAg                                                                               >  2:5065846/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAAAGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  1:2282262/1‑140 (MQ=255)

                                                              aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTACAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                               >  2:1075778/1‑140 (MQ=255)

                                                               cAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATAAGa                                                                              >  1:4761950/1‑140 (MQ=255)

                                                                  tcGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                           >  1:2894550/2‑140 (MQ=255)

                                                                  gcgtactttAATGTAAAATAAACTCGGTCGTTGTATTGTTTTTCCAATTTTATATTTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAAATGAATTTTGATTAAAAGATAATTGATTTTTATCAGaaaa                                                                           <  2:2691909/134‑1 (MQ=255)

                                                                  gcgcattttAATGTAAAACACAGTCGGTCGGTGTAGTCTTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTAAAAATCAACCCGAGCCTTTTTGTAAAATGAATGTTGTTTAAAAGATAAGTGATTTTTATCATaaaa                                                                           <  2:1486948/134‑1 (MQ=255)

                                                                  gcgcAATTTAATGTAAAACAAAGTCGGTCGGTTTATTCGTTTAACAATTTTATAATTAATTGGTAGACCCAATCAAAATCAACCCGCGCATTTTTGTAAAATGAATGTTGATTAAAACATAAGTGATTTTTATCAGaaaa                                                                           <  2:2887721/140‑1 (MQ=255)

                                                                  gCGTAATTTATTTTAAATGAATGTGGGTCGTTTTATTCGTTTTCAAATTTTCTATTTATTTGGTATAGCCACTAAAAATCAACACGAGCCTTTTTTTAAAATTAATGTTTATTAAAATATATTTTATTTTTATCACaaaa                                                                           <  2:856337/140‑1 (MQ=255)

                                                                  gCGGATTTTAATTTAAAAGAAAGTGGGTCGGTTTTTTGGTTTTCAAATTTTCTATTTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGTTTAAAAGATACGTGATTTTTATCAGaaaa                                                                           <  2:1211279/140‑1 (MQ=255)

                                                                  gCGGAATTTATGTTAAAAGAACGTCGTTCGGTGTATTCGTTTTCAAATTTTATAATTAATTGGTATAGCCACAAAAAATCAACACCAGCCTTTTTGTAAAATGAATGTTGATTAAACGTTAAGTGTTTTTTATCAGaaaa                                                                           <  2:562147/140‑1 (MQ=255)

                                                                          tAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGTTTAAAAGATAAGTGATTTTTATCAGAAAACGACCCta                                                                   >  1:4978390/1‑140 (MQ=255)

                                                                                aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                             >  2:4483252/1‑140 (MQ=255)

                                                                                aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                             >  2:750775/1‑140 (MQ=255)

                                                                                aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                             >  2:1678385/1‑140 (MQ=255)

                                                                                         tcggtcggTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATACGTGATTTTTATCAGAAAACGACCCTATACCGACTgaagaga                                                    >  2:4672300/1‑138 (MQ=255)

                                                                                                   tATTCTTTTTCCAATTTTCTAATAAATTGGTAGAGCCACTCAAAATCAACCCGAGCATTTTTGTAAAATGAATGTTGATTAAAAGTTAAGTGTTTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                          <  2:2332963/140‑1 (MQ=255)

                                                                                                          tttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGtgtg                                   >  1:1017137/1‑140 (MQ=255)

                                                                                                           ttCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTTATTTTTATCAGAAAACTACCCTATACCTACTGAAGAAAAAAGGCCAAAAGGTTTgg                                  >  1:4378143/1‑140 (MQ=255)

                                                                                                           ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  2:483669/1‑140 (MQ=255)

                                                                                                           ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  2:5797002/1‑140 (MQ=255)

                                                                                                           ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                  >  2:4274636/1‑140 (MQ=255)

                                                                                                            tCCAATTTTCTAATTATTTGGTAGACCCACTCAAAATCAAAACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGt                                 <  2:2739206/140‑1 (MQ=255)

                                                                                                             ccAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGtt                                >  2:681847/1‑140 (MQ=255)

                                                                                                                 ttttATAATTAATTGTTAGACCCACAAAAAAACAACACGAGCATTTTTTTAAAATCAATTTTTATAAAAAGATAAGTGTTTTTTAACACAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttg                            <  1:141787/140‑1 (MQ=255)

                                                                                                                 tttcataattaatTGGTAGCGCCACTAAAAAACAACACGAGCCTTTTTGTAAACTTAATGTTTATTAAACTATAAGTTATTTTTAACAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttg                            <  1:2849989/135‑1 (MQ=255)

                                                                                                                        tttatttGGTAGACCAACAAAAAACCAACCCGTGCCTTTTTGTAAAATGATTGTTTATTAAAACATACGTTATTTTTAACACAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGctct                     <  1:483669/139‑1 (MQ=255)

                                                                                                                             ttGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttg                >  2:2995352/1‑140 (MQ=255)

                                                                                                                                gTAGAGCCAATCAAAATCAACCCGAGCCTTTTTGTAAAAGTAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttgttg             <  2:1017865/140‑1 (MQ=255)

                                                                                                                                  cccccaaCTAAAAACAAACACGCTCATTTTTGTAAAAGTAATGTTTATTAAAAGATAATTTTTTTTTAACACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  1:660487/134‑1 (MQ=255)

                                                                                                                                  atccccACTCAAAATCACCACGAGCCTTTTGTTAAAATTAATGTGTATTAAACTATACGTGATTTTTATAAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  1:3421253/136‑1 (MQ=255)

                                                                                                                                  agcgCCAATCAAAAACAACACGCGCATTTTTGTAAAATCATTTTTTATTAAAACATAAGTTATTTTTATCATAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  1:1358522/137‑1 (MQ=255)

                                                                                                                                  agagCCACTCAAAATCAACCCTCGCGTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  2:5745493/140‑1 (MQ=255)

                                                                                                                                  agagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  2:3855172/140‑1 (MQ=255)

                                                                                                                                  agagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  2:1731686/140‑1 (MQ=255)

                                                                                                                                  agagCCACTCAAAATAAACACGAGAATTTTTGTAAAATGACTGTTGATTAAAAGATAATTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  2:499359/140‑1 (MQ=255)

                                                                                                                                  agagCCACTAAAAATCAAAACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  2:2599777/140‑1 (MQ=255)

                                                                                                                                  agagCCACACAAAATCAACACCCGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  2:3415308/140‑1 (MQ=255)

                                                                                                                                  agagCCACACAAAAACAACACCCCCCTTTTTGTAAAATGATTGTTGATTAAAAGATAAGTTATTTTTAACACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  1:4679395/140‑1 (MQ=255)

                                                                                                                                  agagCCACAAAAAATCAACACTCGCCTTTTTGTAAAATGATTTTTTATGAAAAGCTATGTGATTTTTATCAGAAAACTACCCTCGACCGACTGAAGAAAACAGGCCAACAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  2:1693201/140‑1 (MQ=255)

                                                                                                                                  agagCAACTCAAAATCAACACGAGCCTTTTTGTAAAATTAATTTTGATTAAAAGATACGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  1:4199090/140‑1 (MQ=255)

                                                                                                                                  agacCCACACAAAAACACCCCGCGCCTTTTTGAAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  1:3842687/140‑1 (MQ=255)

                                                                                                                                  acagCCACTCAAAATCAACACGAGCCTTTTTTTAAAATGATTGTTGATAAAAAGAAAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa           <  1:2767847/138‑1 (MQ=255)

                                                                                                                                   gagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCAt          >  1:1750068/1‑140 (MQ=255)

                                                                                                                                    agCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATa         >  1:1866158/1‑140 (MQ=255)

                                                                                                                                      ccACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACt       >  1:2297449/1‑140 (MQ=255)

                                                                                                                                         cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAATGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa    >  1:953407/1‑140 (MQ=255)

                                                                                                                                          tCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCGTCTTGTTGCATACTTCaa   >  1:3936623/1‑140 (MQ=255)

CTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAA  >  NZ_CP034943/3280202‑3280480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`