Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,636 G→T 6.2% R4I (AGA→ATA)  cysE → serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,6360GT 6.2% 1542.4 / 11.3 569R4I (AGA→ATA) cysEserine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (140/370);  new base T (56/2);  total (196/373)
Fisher's exact test for biased strand distribution p-value = 3.14e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 571 total aligned reads displayed.

GGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAAT  >  NZ_CP034943/112497‑112773
                                                                                                                                            |                                                                                                                                         
 gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                                                                          <  1:859274/140‑1 (MQ=255)
 gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                                                                          <  1:292158/140‑1 (MQ=255)
 gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCAAAGAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                                                                          <  2:4578701/140‑1 (MQ=255)
    agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATg                                                                                                                                       <  2:642817/140‑1 (MQ=255)
      cAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAACAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCt                                                                                                                                     >  2:869996/1‑140 (MQ=255)
      cAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAGGCATGTGTTTTTTAGAATGCt                                                                                                                                     >  2:3317239/1‑140 (MQ=255)
         aaGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGCAATCAAATAGAGAAGTGGGGGGAACCATGTGTTTTTTATAATGCTCaa                                                                                                                                  >  2:4069686/1‑140 (MQ=255)
             cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                                                              <  1:2793899/140‑1 (MQ=255)
             cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                                                              <  1:4934921/140‑1 (MQ=255)
             cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAACCATGTGTTTGTTAGAATGCTCAaagaa                                                                                                                              <  1:4630307/140‑1 (MQ=255)
             cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTGATTGGGTGAAGAGGAAAGCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                                                              <  1:3055811/140‑1 (MQ=255)
             cTGCTTTTCAAACAGAGTCGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                                                              <  1:1811797/140‑1 (MQ=255)
              tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAACAAATAGAGAAGTGGGGGGGAGCATGTGTTTGTTTTAATGCTCAaagaaa                                                                                                                             >  1:3858967/1‑139 (MQ=255)
              tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGAAGAGGAAATCAAATAGAGAAGTTGGGGGACGCCGGTGTTTTTTTTAAATCTCAaagaag                                                                                                                             >  1:2722984/1‑140 (MQ=255)
              tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTGGGTGGAGGGGGAAACCAATAGAGAAGGGGGGGGGAGCATGTGTTTTTTTGTATTCTCAaagaag                                                                                                                             >  1:1927913/1‑140 (MQ=255)
               gCTTTTCAAACAGCGTGGAACCGCGCTGTGTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATACAGAAGGGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  1:5076668/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGGGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  2:4839007/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGGGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  2:2363702/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCTGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  1:2590946/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTCTTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  2:1134443/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  1:525860/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  2:3064496/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  1:3952146/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  2:5788285/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  2:5428148/140‑1 (MQ=255)
                cTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGTAATCAAATCTAGAAGTGGGGGGAAGCATGTGTGTTTTAGAATGCTCAAACAAGAc                                                                                                                           >  2:1423663/1‑140 (MQ=255)
                   ttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACAtt                                                                                                                        <  2:5629422/140‑1 (MQ=255)
                    tCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTg                                                                                                                       <  2:5693755/140‑1 (MQ=255)
                       aaCAGAGTGTAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGATATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  2:2228762/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGACATCACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  1:1077712/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  2:4372229/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  1:564014/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  1:642991/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  1:1663183/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  2:1721340/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  2:1820456/140‑1 (MQ=255)
                        aCAGTGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTATTGGGTGAAGAGGAAAACACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                    <  1:1015190/140‑1 (MQ=255)
                         cAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACa                                                                                                                  <  2:2176580/140‑1 (MQ=255)
                            agTGGAACCGCGCGGTTTAAGCGTCTCTGGCAAGATTTCATGCCCAGGCGCTGTTTGTAGTGTGTGAAAAAGACAACCAATATAGTAGAGGGGGGAAGCCTGTGTTATTTAGAAATATCAACTAAGACATAGATACAgtg                                                                                                               >  2:5156386/1‑140 (MQ=255)
                             gTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGGGATTGGAAATCAAATAAAGAAGTTGGGGGGAGCCAGGTTTTTTTATTATTCACAAATAACACAATtataaaaggt                                                                                                              >  1:3775340/1‑134 (MQ=255)
                                 aaCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACATTGTTCGa                                                                                                          >  2:3395366/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTTAAGCGGTAATCAAATTGAGAAGTGGGGGGAAGCCTGTGTTTTTTATAATGCGACAAAAAGACAATGATAAAGTGTTGGATCAAg                                                                                                     >  1:3647738/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTTTAAAGGAAAAAAAAAAAATAAGTTGGGGGAAGCAATTGTTTTTTTTAAATCTCAAAGAAAACAATTATAAAGTTGTAGATCAAg                                                                                                     >  1:4704036/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATCCAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTTGAATGCTCAAAGAAGACATTGATACCTTGTTCGATCaat                                                                                                     >  2:4225705/1‑139 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAAAAAAAAATAGAACAGGGGGGGAGCAAGTGTTTTTTATTATTCTCAAACAATAAAATGTAAAAGTATTCGATCAAg                                                                                                     >  1:3558201/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGACGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGGGTTTTTTAGAAACCTCAAAGAAGAAAGTGATACAGTGTTAGAACAAg                                                                                                     >  2:3674277/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTTTAATTCTCAAATAATAAATTTATAAAGTGTTCGATCAAg                                                                                                     >  2:2089501/1‑140 (MQ=255)
                                        cgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGAt                                                                                                   <  1:5378792/140‑1 (MQ=255)
                                                 cGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAgcagca                                                                                          <  1:2023989/140‑1 (MQ=255)
                                                   tctcTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaag                                                                                        <  1:772569/140‑1 (MQ=255)
                                                    ctctGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTTGGGGGAGAGGGAATCCAATAGAGAAGTGGGGGGTAGCATGGGTTTTTTTTAATTGTGAAATTAGACATTGGTACAGTGTTAGATGAAGATCCAGCACCaaaa                                                                                       >  1:2152656/1‑138 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGAAGTGGAAATCAAATATAGAAGTGGGGGGAAGCAAGTGTTTTTTTGTATGGGCAAAAAAGACAATGATACAGTGTTCGATCAAGATCCAGCACCaagaa                                                                                      >  1:4488194/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTTTTTTTTATAATGCTCAAAGAAGACAATTATACAGTGTTAGATAAAAATCCAGCAGCaagaa                                                                                      >  2:731808/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGTGGGAATGAAATATATTAGTGGGGGGTAGCATTTGTTTTTTATAATTGTCAAATAATACATTTATACAGTGTTAGATAAATATAAAGCAGCaaaaa                                                                                      >  1:1257246/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGTAATCAAATAGAGAAGGGGGGGGCAGCAGGTGTTTTTTAGAATTCTCCAAGTAGACATTGGTTCAGTGTTGGGGCAAGTTCCAGCAGCcagac                                                                                      >  2:5542908/1‑139 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCCAATAGAGCAGTGGGGGGAAGCCTGTGTTTTTTAGAATTCTCCAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                                                      >  2:820885/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGATAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTAGATCAAGATGCAGCAGCaagaa                                                                                      >  2:3850402/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGACGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                                                      >  2:3494311/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCCTGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                                                      >  2:2706780/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                                                              >  2:4463352/1‑100 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACAATGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                                                      >  2:1617014/1‑140 (MQ=255)
                                                      ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAACCAATAGAGAAGTGGGGGGAAGCATGTCTTTTTTAGACTTCTCAAAGAAGACATTGATACAGTGTTGGATCAAGATCCAGCAGCaagaag                                                                                     <  1:3195905/140‑1 (MQ=255)
                                                            tGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTTTTACAGTGTTCGATCAAGATACAGCAGCAACAAGTTAttt                                                                               >  2:1537240/1‑140 (MQ=255)
                                                              ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                            <  1:341610/93‑1 (MQ=255)
                                                                      cAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGAtt                                                                     <  1:4555497/140‑1 (MQ=255)
                                                                         agGCGCTTTTTTTATTGGGTGTAGAGGAAAAAAAATAGAGAATTGGGGGGAATCAGGTGTTTTTTAGAATTCTTAAATAAGACATTGAAAAATTTTTACAAAAATTAACACCAGCAACAACTTATTTTTAAGTGATTTTa                                                                  >  2:3943689/1‑140 (MQ=255)
                                                                           gcgcTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAAc                                                                <  1:2415832/140‑1 (MQ=255)
                                                                                       aTTGGGTGAAGAGGAAATCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCGAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGtt                                                     <  1:5493732/140‑1 (MQ=255)
                                                                                         tGGGTGAAGAGGAAATCAAATAGAGACGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                                                   <  1:2838722/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGACATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  2:3213666/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAACTCAACTAGAGCAGTGGGGGGAAGCATGTGTTTTTGAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  1:4159610/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAACTCAACTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  1:2080651/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAAATCACATAGAGAAGTTGGGGGAAGCATGTGTTTTTTAGAATGCTCAAATAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  1:582186/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  1:3348841/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  2:5047957/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  1:1855086/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  2:4744681/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  2:1937506/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  1:4261451/140‑1 (MQ=255)
                                                                                           ggTGAAGAGGAAATCAAATAGACAAGGGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  1:4020150/140‑1 (MQ=255)
                                                                                              gAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGc                                              <  1:5748101/140‑1 (MQ=255)
                                                                                                agagGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCta                                            >  1:4354084/1‑140 (MQ=255)
                                                                                                     aaaTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATgg                                       >  2:414973/1‑140 (MQ=255)
                                                                                                       aTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATgg                                       >  1:4418064/1‑138 (MQ=255)
                                                                                                          acaTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTCTTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCa                                  <  2:4950963/138‑1 (MQ=255)
                                                                                                          aaaTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCa                                  <  2:2820084/140‑1 (MQ=255)
                                                                                                            aTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATc                                >  1:1508737/1‑140 (MQ=255)
                                                                                                               gcgacgTGGCGCGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                             <  1:4787462/135‑1 (MQ=255)
                                                                                                                     tGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                       <  1:1407386/140‑1 (MQ=255)
                                                                                                                     tGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATCGGCGCATCGGATTGcac                       <  1:514247/140‑1 (MQ=255)
                                                                                                                     tGGGGGGAACCATGTGTTTTTTAGAATGCTAAAAGAAGACATTGATACAGTTTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                       <  2:2936304/140‑1 (MQ=255)
                                                                                                                        ggggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATg                    <  2:2542275/140‑1 (MQ=255)
                                                                                                                          ggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcaca                      <  2:3571916/136‑1 (MQ=255)
                                                                                                                          ggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCt                  >  1:989687/1‑140 (MQ=255)
                                                                                                                          ggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCt                  <  2:1257246/140‑1 (MQ=255)
                                                                                                                            aaGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATGTTAACTTATTCAGGTTTACATGCTATATGTGCGCATCGGATTGCACATGCttt                >  1:715159/1‑140 (MQ=255)
                                                                                                                               cATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTtata             >  2:1192163/1‑140 (MQ=255)
                                                                                                                                  gtgtTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGAATCAAGAAGTTATTTTGAAGTTATTTTAACTTATTCCGGTTTACATGATATATGGGCGCATCGGATTGCACATGCTTTATATaa          >  2:3455609/1‑140 (MQ=255)
                                                                                                                                      tttttAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                 <  2:5307177/97‑1 (MQ=255)
                                                                                                                                       ttttAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGaa     <  1:3500836/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
GGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAAT  >  NZ_CP034943/112497‑112773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: