Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,349,666 T→G 7.4% intergenic (+1/+26) EO946_RS17305 → / ← padC hypothetical protein/phenolic acid decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,349,6660TG 7.4% 776.4 / 7.1 365intergenic (+1/+26)EO946_RS17305/padChypothetical protein/phenolic acid decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (219/98);  new base G (22/22);  total (242/123)
Fisher's exact test for biased strand distribution p-value = 1.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

TTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAG  >  NZ_CP034943/3349529‑3349804
                                                                                                                                         |                                                                                                                                          
ttttGTCGGCCGTTTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATtc                                                                                                                                          <  2:1731609/140‑1 (MQ=255)
     tCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAATGAAAACCCGGGAAAGAGAAGTGATTGTCgtt                                                                                                                                     >  2:3847587/1‑140 (MQ=255)
     tCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCttt                                                                                                                                     >  2:1783919/1‑140 (MQ=255)
      cGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCtttt                                                                                                                                    <  1:4298904/140‑1 (MQ=255)
        gCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTcc                                                                                                                                  <  1:2580624/140‑1 (MQ=255)
         ccGTGTTATGTTTACGGGACACAGCCTAGTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAACAAGAAAACCCGGGAAAGAGAAGGGATTCTCTTTTCCg                                                                                                                                 <  1:210621/140‑1 (MQ=255)
                   tttACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATg                                                                                                                       <  1:688805/140‑1 (MQ=255)
                    ttACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGt                                                                                                                      >  1:2298434/1‑140 (MQ=255)
                      aCGGGACACAGCCTGTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGttt                                                                                                                    >  1:5084588/1‑140 (MQ=255)
                      aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGACTTATGATGGTTCCCGCATACTCAGCAAAAAGAATACCCGGGAAAGAGAAGTGATTCTCTGTTCCGGGTTTTTATGttt                                                                                                                    >  1:5143956/1‑140 (MQ=255)
                      aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGttt                                                                                                                    >  1:917349/1‑140 (MQ=255)
                             acaGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATaa                                                                                                             >  2:163862/1‑140 (MQ=255)
                             acaGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATaa                                                                                                             >  1:2499572/1‑140 (MQ=255)
                               aGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTCTGTTTATTATAAtt                                                                                                           >  1:4417360/1‑140 (MQ=255)
                                   tagtACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:3921627/137‑1 (MQ=255)
                                   tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:3321347/140‑1 (MQ=255)
                                   tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:5245479/140‑1 (MQ=255)
                                    aTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTccc                                                                                                      >  1:534482/1‑140 (MQ=255)
                                    aTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTccc                                                                                                      >  1:5205301/1‑140 (MQ=255)
                                     ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  2:194572/140‑1 (MQ=255)
                                     ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  2:861822/140‑1 (MQ=255)
                                     ttACCAGCCTATGCGCGGGACTGGCATCCTACTGTTTTTCGCGGGAATTATGCTGGGTCCCGCATACTCAGCAAACAGAAAACCCGGGCACGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:4506305/140‑1 (MQ=255)
                                         cAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTACcgcgcg                                                                                                 >  1:3860582/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAATGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:343698/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCGGGGGTGTTATGTTTCTTATAATTTTCCCGCGCGta                                                                                               >  1:1826831/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     >  1:4678697/1‑134 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:999008/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:5428523/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:464402/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:454067/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:4031975/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:1544305/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTCCCCGCGCGta                                                                                               >  2:1112712/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTAGGTTTATTATAATTGTCCCGCGCGta                                                                                               >  1:1195154/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTGATGGTGATTATAATTTTCCCGCGCGta                                                                                               >  1:3632144/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCGTTTCCGGGTTTTTATGTGTATTATAATTTTCCCGCGCGta                                                                                               >  1:3072449/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:4540377/1‑140 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGACTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:1281350/132‑1 (MQ=255)
                                           gCCTATGCGCTGGACTGGCATCATACTGTTTTTAGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTAGGTTTATTATAATTTTCCcgcgcgga                                                                                               >  1:1052539/1‑138 (MQ=255)
                                             cTATGCGCTGGACTGTCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGtata                                                                                             <  2:3342196/140‑1 (MQ=255)
                                                tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGACAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATACTTTTCCCGCGCGTATATCg                                                                                          >  1:2997859/1‑140 (MQ=255)
                                                tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:273583/129‑1 (MQ=255)
                                                tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:238629/1‑140 (MQ=255)
                                                tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACGCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  2:717750/1‑140 (MQ=255)
                                                tGCGCTGGACTGGCATCATACTGTTTTTCGCGCGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTGTATTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  2:4365255/1‑140 (MQ=255)
                                                tGCGCTGGACTGGCATCATACGGTTTTTCGCGGGAATTATGCTGGTTCCCGCCTACGCAGCAAAAACAAATCCCGGGAAACAGAAGTGAGGCGCTTTTCCGGGTGGGTATGGTTCTGATCATTTTCCCGCGCGTATCTCg                                                                                          >  1:2084014/1‑140 (MQ=255)
                                                       gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCTCAGACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                   >  1:4338551/1‑140 (MQ=255)
                                                       gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTttat                                                                                                                  <  1:1762194/109‑1 (MQ=255)
                                                       gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                   >  2:60699/1‑140 (MQ=255)
                                                       gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                   >  1:140573/1‑140 (MQ=255)
                                                         cTGGCATCCTACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAACACCCGGGAACGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  2:5775663/140‑1 (MQ=255)
                                                         cTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  2:3498114/140‑1 (MQ=255)
                                                           ggCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTcc                                                                               <  1:201598/140‑1 (MQ=255)
                                                            gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              >  2:1241370/1‑140 (MQ=255)
                                                            gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTGCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              >  2:1578515/1‑140 (MQ=255)
                                                            gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              >  1:3012884/1‑140 (MQ=255)
                                                               tcatCCGGTTTTTCGCGGGAATGATGATGGTTCCCGCATACTCAGCCAAAAGAAAACCCGGGCAAGAGAAGTGATGCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGGGTATATCGTCTGTCATTCCTTCa                                                                           >  2:1825139/1‑140 (MQ=255)
                                                                 atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  2:5569299/132‑1 (MQ=255)
                                                                 atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATa                                                                         >  2:484437/1‑140 (MQ=255)
                                                                      gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAt                                                                                                            >  1:5585786/1‑100 (MQ=255)
                                                                      gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                    >  2:4774570/1‑140 (MQ=255)
                                                                      gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                    >  2:2231783/1‑140 (MQ=255)
                                                                      gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                    >  2:3120899/1‑140 (MQ=255)
                                                                       tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGc                                                                   >  1:948985/1‑140 (MQ=255)
                                                                       tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGc                                                                   >  2:3048381/1‑140 (MQ=255)
                                                                          ttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTGTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTAGATCGTCTGTCATTCGTTCATAAGGAGCttt                                                                >  2:5444892/1‑140 (MQ=255)
                                                                            cgcgGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTGTATGTGTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCCTAAGGAGCTTTCg                                                              >  2:3765107/1‑140 (MQ=255)
                                                                                       tgatgGGTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTActtct                                                   <  1:3148227/140‑1 (MQ=255)
                                                                                           ggTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGGTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGt                                               >  1:4206819/1‑140 (MQ=255)
                                                                                            gTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGtt                                              >  1:2191526/1‑140 (MQ=255)
                                                                                            gTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTAGGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGtt                                              >  2:2772524/1‑140 (MQ=255)
                                                                                             ttCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATGCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGTGCTTTCGAAATTACTTCTTCGTTg                                             >  2:5629817/1‑140 (MQ=255)
                                                                                             ttCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGGTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTg                                             >  2:795004/1‑140 (MQ=255)
                                                                                               cccGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                           >  1:3389562/1‑140 (MQ=255)
                                                                                               cccGCATACTCAGCAAAAAGAAAACCCGCGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTAGATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                           <  1:3708323/140‑1 (MQ=255)
                                                                                                 ctcATACTCTGCAAAAAGAAACCCCGGGACACAGAAGTGAGTCTGTTTTCAGTGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCTTTCCTTCATAAGGAGCTTTCGAAATTACTTCTTAGTTGTCGa                                         <  2:4683156/138‑1 (MQ=255)
                                                                                                   cATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACt                                       >  1:1521216/1‑140 (MQ=255)
                                                                                                        tCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTc                                  >  2:4214038/1‑140 (MQ=255)
                                                                                                          aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  1:2608574/1‑140 (MQ=255)
                                                                                                          aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  2:5347035/1‑140 (MQ=255)
                                                                                                          aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  1:3813846/1‑140 (MQ=255)
                                                                                                          aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCGTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  2:231/1‑140 (MQ=255)
                                                                                                          aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTCGCGCGCGTATATCGTCTGTCATTCGTTCATAAGGCGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  2:4341351/1‑140 (MQ=255)
                                                                                                          aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCGCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTGTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  2:2829367/1‑140 (MQ=255)
                                                                                                            cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAttt                              >  1:760530/1‑140 (MQ=255)
                                                                                                            cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGGTTTTATGTTTATTATAATTTTCCCGCGCGTCTATCGTCTGTCATTCCTTCATACGGAGCTTTCGAAATTCCTTCTTCGTGGTCGACTCCCTCAgtt                              >  2:4992293/1‑140 (MQ=255)
                                                                                                                aaGAAAACCCGGGAACTAGAAGTGATTCTCTTTTCCGGGTATTTATGTTTATTATAAATTTCCCTCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCa                          <  1:3508046/140‑1 (MQ=255)
                                                                                                                  gAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGa                        <  2:427762/140‑1 (MQ=255)
                                                                                                                    aaaCACGGGAAAGAGAAGTGCTTCGATTTTCCTTGTTTTTATGTTTATTATAATTTTCACGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGACATTACTTCTTCGTTGTAGACTCCCTCATTTTTAAGaaa                      >  2:2442227/1‑140 (MQ=255)
                                                                                                                      aCCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAgg                    >  1:4063837/1‑140 (MQ=255)
                                                                                                                           ggAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAAtc               >  1:286198/1‑140 (MQ=255)
                                                                                                                                 agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         <  1:163862/140‑1 (MQ=255)
                                                                                                                                 agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         <  2:2090773/140‑1 (MQ=255)
                                                                                                                                 agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         <  2:1100818/140‑1 (MQ=255)
                                                                                                                                 agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATGTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         <  1:486837/140‑1 (MQ=255)
                                                                                                                                  gaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCaa        >  1:4761733/1‑140 (MQ=255)
                                                                                                                                      tGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACCTCTTCGTTGTCGACTCCCTCATTTTTCa                          <  1:1344802/118‑1 (MQ=255)
                                                                                                                                      tGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACATCTTCGTTGTCGACTCCCTCATTTTTCa                          >  2:1344802/1‑118 (MQ=255)
                                                                                                                                        aTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAg  >  2:3856719/1‑140 (MQ=255)
                                                                                                                                        aTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAg  >  2:2701299/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAG  >  NZ_CP034943/3349529‑3349804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: