BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,362,393	G→C	11.0%	L99L (CTG→CTC)	EO946_RS17380 →	MazG‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,362,393	0	G	C	11.0%	913.8 / 27.5	346	L99L (CTG→CTC)	EO946_RS17380	MazG‑like family protein
Reads supporting (aligned to +/- strand): ref base G (207/90); new base C (2/43); total (210/136)
Fisher's exact test for biased strand distribution p-value = 8.46e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 348 total aligned reads displayed.

TGAGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGC  >  NZ_CP034943/3362255‑3362531

tgaGAAAGAATCGACACGAGCTGAGCAAAATCAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATAGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACTTGATGAAAGCCCACCAAGAAAAAATGa                                                                                                                                           >  1:3776008/1‑140 (MQ=255)

  agaaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGAcc                                                                                                                                         >  2:5527645/1‑140 (MQ=255)

    atataaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaa                                                                                                                                       <  1:258209/136‑1 (MQ=255)

    aaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaa                                                                                                                                       <  1:2842292/140‑1 (MQ=255)

     aagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGTAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaaa                                                                                                                                      <  2:1422165/140‑1 (MQ=255)

     aagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaaa                                                                                                                                      <  1:3115558/140‑1 (MQ=255)

         aTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGTAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGGAGACGGGATGAACGCGCTCCAAGACACACTGACCacacctg                                                                                                                                  >  2:4373398/1‑137 (MQ=255)

         aTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGtt                                                                                                                                  >  2:20594/1‑140 (MQ=255)

              cacGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTGTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGACAAACTGACCAAACGTTTTGAg                                                                                                                             >  1:3680180/1‑140 (MQ=255)

                 gAGCTGAGCAAAAACAAGAAGTGATTGAAGAAATGGGGGATCTCATCGGAAATATAGCCATTCTTGATTATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCACACGTTTTGAgcat                                                                                                                          <  2:5041140/140‑1 (MQ=255)

                       aGCAAAAACAAGAATTGATTGAATAAATGGGGTATGTCATCGGAAATATCGCCATTCATGCTGATATGTACGGAGTCTCTTTAGAATACGTGATGAAATCCCACCAAGAAAAACTTACCAAACGTTTTGAGCATGAATaa                                                                                                                    >  1:2658829/1‑140 (MQ=255)

                       aGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATaa                                                                                                                    >  2:4821908/1‑140 (MQ=255)

                            aaaCAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAg                                                                                                               >  2:4714717/1‑140 (MQ=255)

                             aaCACGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGc                                                                                                              >  1:5406773/1‑140 (MQ=255)

                              aCAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCg                                                                                                             >  2:1956687/1‑140 (MQ=255)

                                 aGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGAGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTTAGCATGCATAAAAAAGCTGcc                                                                                                          >  2:4314207/1‑140 (MQ=255)

                                 aGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATGCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGcc                                                                                                          >  1:2312605/1‑140 (MQ=255)

                                        attgaAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATgg                                                                                                   >  1:3447235/1‑140 (MQ=255)

                                           gaagaaCTGGGGCATGTCAACGTAACTATCGCCATTCTTGCTGATATGTACGGTGACTCTTTAGAAGACGTGATGAAAGCCCACCAACAAAAACTGACCACACGTGTTGAGAATGCAAAAAAAAGCGGCCGTAATGGCCg                                                                                                <  2:5769430/140‑1 (MQ=255)

                                             agaaATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCt                                                                                              >  1:4463818/1‑140 (MQ=255)

                                                  tGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCtttttt                                                                                         >  2:2588322/1‑140 (MQ=255)

                                                    ggggATCTCAACGTAAATTGCGCAATTCTTGCTGATATTTACGGAGTCTATTTACAAGACGTGTTGAACGCCCAACAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTa                                                                                       <  2:4647480/140‑1 (MQ=255)

                                                        aTGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCt                                                                                   >  1:5635405/1‑140 (MQ=255)

                                                         tGTAATCGGAAATATCGACTTTCTTGCTGATATGTACGGAGTCTCTTTAGAACAGGTGATGAACGCCCACCAAGAAAAAGTGACCAAACTTTTTGAGCATGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCtg                                                                                  <  1:5526235/140‑1 (MQ=255)

                                                            caccgcaaaTAGCGCCATTTTTGCTTATATGTACGTAGTCTATTTAGAACCCGTGATCAAAGCCAACCAACAAAAACTCACCAACCTTTTGGAGAAGCAAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGa                                                                               <  1:1714113/134‑1 (MQ=255)

                                                            caccGGACATATCGCAATTGTTGCTTATATGTCCGGAGTCTCTTTAGAAGACGTGATCAAAGCCCACAAATAAAAACTCACCAAACGTTTTGAGCATGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGa                                                                               <  1:752705/137‑1 (MQ=255)

                                                            cATGGGAAATATCGCCATTCTTGCTGATATGTCCGGATTCTCTTTAGAACACGTGATGAACGCCCACCAAGAAAACCTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGa                                                                               <  1:5234534/140‑1 (MQ=255)

                                                            cATCGTAAATATCGCAATTTTTGATTATTTGTACGGATTCTATTTACAAGACGTGATCAAAGCCCACCAACAAAACCTCACCAACCGTTTTGAGCATCAATAAAAACGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGa                                                                               <  1:315410/140‑1 (MQ=255)

                                                            cATCGGAAATATCGCCTTTCTTGCTGATATTTACGGAGTCTCTTTACAAGCCGTGATGAACCCCAACCAACAAAACCTGACCAAACTTTTTGACCATGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGa                                                                               <  1:147568/140‑1 (MQ=255)

                                                              tCGGAAATATCGCCATTCTTGCTGATATGTCCGGAGTCTCTTTAGAAGACTTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGAtt                                                                             <  2:1738449/140‑1 (MQ=255)

                                                              tCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAACTGTGAtt                                                                             >  1:3552945/1‑140 (MQ=255)

                                                                 gAAATACCCCCATTCTTGCTGATTGCTCCGGAGTCTCTTTACAACACGTTAACAAACCCCACCACCAAAAACTGACAAACCTTTTTGAGCCTGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTtca                                                                          <  1:2443769/140‑1 (MQ=255)

                                                                   aaTATCGCCTGGCGTGCTGATCGGTCCGGAGTCTCTTTAGAAGACGTGAAGAACGCCCACCAAGAAAAACTGCCCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTtcatc                                                                        <  2:3676123/140‑1 (MQ=255)

                                                                      atCGCATTTCTTGCTGATATGTACGGAGTCTCTTTATAAGACGTGATCAAACCCCACCAAGAAAAACTGACAAAACTTTTTGAGATTGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATg                                                                     <  1:1539762/140‑1 (MQ=255)

                                                                           ctttCTTGCTGATTTGTACGTAGTCTCTTTAGAAGACGTGATGAAACCCCACCAAGAAAAACTCCCCAAAGGTTTGCAGCATGCATAAAACAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGAt                                                                <  1:2065408/138‑1 (MQ=255)

                                                                           cATTCTTGCTGATAGGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAACCTGACCAAACGTTTTGACCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGAt                                                                <  2:1367317/140‑1 (MQ=255)

                                                                            aTTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTGTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCGGTGATTTCATCATGGTGATc                                                               >  1:3324620/1‑140 (MQ=255)

                                                                                  gCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTAATCATGGTGATCAAAGaa                                                         >  1:2194917/1‑140 (MQ=255)

                                                                                  gCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGAGTTCATCAGGGTGATCAAAGaa                                                         >  1:3023128/1‑140 (MQ=255)

                                                                                  gCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCGGTGATTTCATCATGGTGATCAAAGaa                                                         >  1:4946331/1‑140 (MQ=255)

                                                                                      atatGTACGGAGTCTCTTTAGAAGAGGTGATCAACGCCCACCAAGAAAAAGTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgc                                                     <  1:428336/140‑1 (MQ=255)

                                                                                      atatGTACGGAGTCTATTTAGAAGAGGTGATGAAAGCCCACCACTAAAACCTGACCACCCGTTTTTAGCATGAAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgc                                                     <  1:3431875/140‑1 (MQ=255)

                                                                                        atttACGGAGTCTATTTAGAAGACGTTATGAAAGCCCACCAAGAAAAACTCACCAAACTTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                   <  2:171199/137‑1 (MQ=255)

                                                                                        atGTCCGGAGTCTAGTTAGAAGACGGGATGAACGCCCACCAAGAACACCTCACCAAACGTTTTGACCAGGCATACAAAAGCGGCCGCAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                   <  2:3482308/140‑1 (MQ=255)

                                                                                        atGTACGGAGTCTCTTTAGAAGACGTGATGAAACCCCACCAACAAAAACGGACCAACCGTTTTGAGCATGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                   <  1:1796352/140‑1 (MQ=255)

                                                                                          cgccGGAGTCTCTTTAGAACCCGTGCTGAAAGCCCACCAAGAACACCTGCCCACCCGTTTTGAGCATCAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGAGTTCATCATGGTGATCAAAGAAATgctgct                                                 <  1:4105954/137‑1 (MQ=255)

                                                                                            aCGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCttt                                               >  1:329177/1‑140 (MQ=255)

                                                                                                aGTCTCTTTAGAAGACGTGATGAAAGCCCACCCAGAAAAACTGACCAAACGTTTGGAGCAGGCATAAAAAAGCGGCCGTAATGGCCGCTTTTGTTAACCGGTGATTTCATCATGGTGATGAAAGATATGCTGCTTTCCtg                                           >  1:3236299/1‑139 (MQ=255)

                                                                                                aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAATGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCTTGGGGATCAAAGAAATGCTGCTTTCCTa                                           >  1:5731541/1‑140 (MQ=255)

                                                                                                aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATTGTGATCAAAGAAATGCTGCTTTCCTa                                           >  2:4168984/1‑140 (MQ=255)

                                                                                                aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                           >  1:2501700/1‑140 (MQ=255)

                                                                                                aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                           >  2:5235983/1‑140 (MQ=255)

                                                                                                aGTCACTTTAGAAGACTTGATTAAACCCCACCAAGAAAAACTCACCAAACGTTTTTAGCAGGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                           <  1:1231706/140‑1 (MQ=255)

                                                                                                  tctcTTTAGAAGACTTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                   <  2:2923273/130‑1 (MQ=255)

                                                                                                  tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                   <  2:4993914/130‑1 (MQ=255)

                                                                                                  tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                   >  1:5607175/1‑130 (MQ=255)

                                                                                                  tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                   >  1:4993937/1‑130 (MQ=255)

                                                                                                  tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                   <  2:5607175/130‑1 (MQ=255)

                                                                                                  tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAtt                                         >  1:1022194/1‑140 (MQ=255)

                                                                                                  tctcTTTAGAAGACGTGATGAAACCCCACCACGAAAAACTCACAAAACGTTTTTAGAATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAtt                                         <  2:1261099/140‑1 (MQ=255)

                                                                                                   ctctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATAAAAGAAAAGCTGCTTTACTAttt                                        >  2:3444996/1‑140 (MQ=255)

                                                                                                     ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTTAGCATGCATAAAAAAGCGGCCGTAATGGCCGATTTTTTTAAACTGTGATTTCATCAGGGTGATCAAATAAAAGATTCTTTCATAGTTtg                                      >  2:4019499/1‑140 (MQ=255)

                                                                                                     ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                      >  2:5685490/1‑140 (MQ=255)

                                                                                                     ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                      >  2:4056493/1‑140 (MQ=255)

                                                                                                     ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                      >  2:3751965/1‑140 (MQ=255)

                                                                                                          gaagaCGTGATGAAAGCCCACCAAGAAAAACGGACCAACCTTTTTCAGCATGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                 <  1:5064997/140‑1 (MQ=255)

                                                                                                          gaagaCGTGATGAAACCCCACCAAGAAAAACTCACCAACCGTTTTGAGCATGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                 <  1:2896850/140‑1 (MQ=255)

                                                                                                              aCGTGATTAAAGCCCACCATGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAgg                             <  2:5204499/140‑1 (MQ=255)

                                                                                                              aCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAgg                             >  2:5458361/1‑140 (MQ=255)

                                                                                                              aCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCGGTGATTTCATGCTGGTGATGAAAGAAATGCTGCGTTCCTATTTTTTTAAATAgg                             >  1:5118238/1‑140 (MQ=255)

                                                                                                               cGTGATGCAACCCCACCACGACAAACTCACCAACCGTTTTGACCATGCTCCAAAAAGCGGCCGTAATGGCCGCTATTTTTAACCGGTGATTTCATCATGGTGATCAAAGAACTGCTGCTTTCCTATTTTGTGAACTAGGc                            <  2:2681662/140‑1 (MQ=255)

                                                                                                               cGTGATGAATGCCCACCAATAAAAACTGACCAAACCTTTTGAGCATGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  2:2243067/140‑1 (MQ=255)

                                                                                                               cGTGATGAAAGCCCACCAAGAAAACCTGACCAAACTTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  1:1813392/140‑1 (MQ=255)

                                                                                                               cGTGATGAAAGCCCACCAAGAAAAACTGACCACACGTTTTGATCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  2:1914921/140‑1 (MQ=255)

                                                                                                               cGTGATGAAAGCCCACCAAGAAAAACTGACCAACCGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  2:3234273/140‑1 (MQ=255)

                                                                                                               cGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  2:3136142/140‑1 (MQ=255)

                                                                                                               cGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  2:5399365/140‑1 (MQ=255)

                                                                                                               cGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  2:2354049/140‑1 (MQ=255)

                                                                                                               cGTGATGAAACCCCACCAAGAAAAACTGACCAACCGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  2:3999441/140‑1 (MQ=255)

                                                                                                               cGTGATGAAACCCCACCAACAAAAACTCACAACCCGTTTTGACCAGGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                            <  1:2016560/140‑1 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAgggc                           >  1:4567808/1‑138 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  1:3491685/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  2:935535/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  2:914298/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  2:1647704/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  1:3866773/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  2:2860481/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  2:1083724/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  2:2506559/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  2:2476299/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                           >  2:1567016/1‑140 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGGGAAATAGGcg                           >  2:4539799/1‑139 (MQ=255)

                                                                                                                gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTACTATTTTGTGAAATAGGcg                           >  2:846593/1‑139 (MQ=255)

                                                                                                                        aGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTAt                   >  1:477569/1‑140 (MQ=255)

                                                                                                                        aGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTAt                   >  2:2092840/1‑140 (MQ=255)

                                                                                                                        aGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATAATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTAt                   >  2:1156738/1‑140 (MQ=255)

                                                                                                                          cccACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgc                 >  2:4216763/1‑140 (MQ=255)

                                                                                                                                     aaaCTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTTCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCtt      >  2:757231/1‑140 (MQ=255)

                                                                                                                                       aCTGACCAACCGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCg    <  2:334164/140‑1 (MQ=255)

                                                                                                                                         tGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGc  >  2:3013220/1‑140 (MQ=255)

TGAGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGC  >  NZ_CP034943/3362255‑3362531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`