BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,362,413	C→A	5.9%	A106E (GCA→GAA)	EO946_RS17380 →	MazG‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,362,413	0	C	A	5.9%	826.9 / 4.1	339	A106E (GCA→GAA)	EO946_RS17380	MazG‑like family protein
Reads supporting (aligned to +/- strand): ref base C (194/109); new base A (4/31); total (198/141)
Fisher's exact test for biased strand distribution p-value = 1.49e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

CTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTGCAGTCCTACAA  >  NZ_CP034943/3362275‑3362550

   aGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATaa                                                                                                                                       >  2:4624334/1‑140 (MQ=255)

   aGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATaa                                                                                                                                       >  2:4821908/1‑140 (MQ=255)

        aaaCAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAg                                                                                                                                  >  2:4714717/1‑140 (MQ=255)

        aaaCAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAg                                                                                                                                  >  2:1097901/1‑140 (MQ=255)

             aGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGATTTGACCATGCATAAAAAATAGGcc                                                                                                                             >  2:4318958/1‑140 (MQ=255)

             aGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATGCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGcc                                                                                                                             >  1:2312605/1‑140 (MQ=255)

             aGAAGTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGAATAAAAAATCGGcc                                                                                                                             >  2:2040419/1‑140 (MQ=255)

                    attgaAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATgg                                                                                                                      >  1:248720/1‑140 (MQ=255)

                       gaagacATGGGGGATTTCATCGGAAATATCGCCTTTCTTGCTGATATTTACGGAGTCTCTTTAGACGACGTGATGAAAGCCCACCAAGAAAAACTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCg                                                                                                                   <  2:1372338/140‑1 (MQ=255)

                                    aTGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCt                                                                                                      >  1:5635405/1‑140 (MQ=255)

                                     tGTAATCGGAAATATCGACTTTCTTGCTGATATGTACGGAGTCTCTTTAGAACAGGTGATGAACGCCCACCAAGAAAAAGTGACCAAACTTTTTGAGCATGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCtg                                                                                                     <  1:5526235/140‑1 (MQ=255)

                                        cttggtaaaTATCGCAATTCTTGCTTATATGTACGGAGTATCTTTATAAGACGTGATCAAACCCCACAAACAAAAACTCACCAAACTTTTTGAGCATCAAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGa                                                                                                  <  1:489827/134‑1 (MQ=255)

                                        caccGGACATATCGCAATTGTTGCTTATATGTCCGGAGTCTCTTTAGAAGACGTGATCAAAGCCCACAAATAAAAACTCACCAAACGTTTTGAGCATGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGa                                                                                                  <  1:752705/137‑1 (MQ=255)

                                        cATCGTAATTAGCGCCATTATTGCTTATTTGTACGGAGTCTCTTTAGACCCCGTGATCAAAGCCAACCAACAAAAACTCCCCAAACTTTTTGAGCAGGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGa                                                                                                  <  1:4545373/140‑1 (MQ=255)

                                         aTCGTAAATAGCCCCATGATTGCTGATATGTACGGAGTCTCTTTAGAACACGTGATGAAACCCCACCAAGAAAAACTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGAt                                                                                                 <  2:5263099/140‑1 (MQ=255)

                                                 tatCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTcatcat                                                                                         >  2:4665444/1‑140 (MQ=255)

                                                  atCGCATTTCTTGCTGATATGTACGGAGTCTCTTTATAAGACGTGATCAAACCCCACCAAGAAAAACTGACAAAACTTTTTGAGATTGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATg                                                                                        <  1:1539762/140‑1 (MQ=255)

                                                    cGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGt                                                                                      >  1:5549860/1‑140 (MQ=255)

                                                       cATTTTTGCTGATATGTACGGCGTCTCTTTACAAGACTTGATGAAAGCCCACCAATAAAAACTCACCAAACGTTTTGAGCAGGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGAt                                                                                   <  1:5710028/140‑1 (MQ=255)

                                                       cATTCTTGCTGATATTTACGGAGTCTCTTTACAAGACGTGATGAAACCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGAt                                                                                   <  2:866577/140‑1 (MQ=255)

                                                       cATTCTTGCTGATAGGGACGGAGTCTCTTTAGCAGACGTGCTGAAAGCCCACCAAGAAAACCGCACCAAACGTGTTCACCATCCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGAt                                                                                   <  1:3307434/140‑1 (MQ=255)

                                                       atttcttcCTTATATGTACGGATTCTCTTTAGACGACGTGATCAACGCCCACCACCAAAAACTGACCAACCGTTTTGAGCATCAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGAt                                                                                   <  1:5010139/138‑1 (MQ=255)

                                                              gCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTAATCATGGTGATCAAATaa                                                                            >  1:2546662/1‑140 (MQ=255)

                                                              gCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTAATCATGGTGATCAAAGaa                                                                            >  1:2194917/1‑140 (MQ=255)

                                                               atGATAGGTACGTATTCTCTTTATAAGACGTGAGGAAAGCCCACCAAGCACAACTGACACAACGTTTTGATAATTCATAAAAAAGCGGCCGTAATGGCCGATATTTTTAACCTGTGATTTAATCATGGTGATCAAAGaaa                                                                           <  2:589772/139‑1 (MQ=255)

                                                                 gATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtg                                                                         >  2:2186039/1‑140 (MQ=255)

                                                                  atatGTACGGAGTCTATTTAGAAGAGGTGATGAAAGCCCACCACTAAAACCTGACCACCCGTTTTTAGCATGAAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgc                                                                        <  1:3431875/140‑1 (MQ=255)

                                                                    atctACGCAGTCTCTTTAGAACACGTCATCAAAGCCCCCCACGAAAACCTCCCCAACAGTATTGAGCATGCAACAAACAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                                      <  1:2849935/137‑1 (MQ=255)

                                                                    atGTCCGGAGTCTAGTTAGAAGACGGGATGAACGCCCACCAAGAACACCTCACCAAACGTTTTGACCAGGCATACAAAAGCGGCCGCAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                                      <  2:3482308/140‑1 (MQ=255)

                                                                    atGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAATCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTAATAATGGGGATCAAAGAAAtgctg                                                                      >  2:166296/1‑140 (MQ=255)

                                                                    atGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAatgatg                                                                      >  2:5437666/1‑140 (MQ=255)

                                                                    atGTACGGAGTATCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                                      <  2:193442/140‑1 (MQ=255)

                                                                         cGGAGTCTGTTAAGAAGCCGTCAGTAACGCCAACAAACTAAAAGTGCCCAAACTTTTTGAGCATCCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACATGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTc                                                                 <  1:3444996/140‑1 (MQ=255)

                                                                            attctcTTTAGAACACTTCCTGAAACCCCACAAACAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTATTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                                              <  2:5480276/138‑1 (MQ=255)

                                                                            aGTCTCTTTAGAAGACGTGATGAAAGCCCACCCAGAAAAACTGACCAAACGTTTGGAGCAGGCATAAAAAAGCGGCCGTAATGGCCGCTTTTGTTAACCGGTGATTTCATCATGGTGATGAAAGATATGCTGCTTTCCtg                                                              >  1:3236299/1‑139 (MQ=255)

                                                                            aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATTGTGATCAAAGAAATGCTGCTTTCCTa                                                              >  2:4168984/1‑140 (MQ=255)

                                                                            aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGTAATGCTGCTTTCCTa                                                              >  1:3527589/1‑140 (MQ=255)

                                                                            aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                                              >  1:377717/1‑140 (MQ=255)

                                                                            aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                                              >  2:5748196/1‑140 (MQ=255)

                                                                            aGTCACTTTAGAAGACTTGATTAAACCCCACCAAGAAAAACTCACCAAACGTTTTTAGCAGGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTa                                                              <  1:1231706/140‑1 (MQ=255)

                                                                            aGTATCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTCACCAAACGTTTTGAGGATGAATAAAAAAGCGGCCGTAATGGCCCCTTTTTTAAACCGGTGATGTCATCCTGGTGATCACAGAAATGCTGCTTTccga                                                              >  2:3325379/1‑138 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAATGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTAATCATGGTGATAAAAGAAATGCTGCTTTCCTAtt                                                            >  1:1806197/1‑140 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCCTGGTGATAAAAGAAATGCTGCTTTCCTAtt                                                            >  1:1773310/1‑140 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAGAGAAATGCTGCTTTCCTAtt                                                            >  2:2547458/1‑140 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                                      >  1:2923273/1‑130 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                                      <  2:4993914/130‑1 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                                      <  2:5607175/130‑1 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAtt                                                            >  1:1022194/1‑140 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATAATGGTGATAAAAGAAATGCTGCTTTCCTAtt                                                            >  1:149049/1‑140 (MQ=255)

                                                                              tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAACTGTGATTTAATCATGGTGATCAAAGAAATTATGCTTTACTAtt                                                            >  1:2129247/1‑140 (MQ=255)

                                                                              actctTTAGAACACGGCATGAAAGCCCACCAACAAACACTCACCACACGTTTTGCGCATGCATAAAACAGCGGCCGTACTGGCCGCTTTTTTTAACCTGTGAGTTCATCATGGTGATCAAAGAAATGCTGCTGTCCTAtt                                                            <  2:4001490/139‑1 (MQ=255)

                                                                                 ctTTAGAATACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGATCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAttttt                                                         >  2:292366/1‑139 (MQ=255)

                                                                                 ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                                         >  2:3751965/1‑140 (MQ=255)

                                                                                 ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                                         >  2:4056493/1‑140 (MQ=255)

                                                                                 ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                                         >  2:4451240/1‑140 (MQ=255)

                                                                                      taagaCTTTATTAAAGCACAAAAAGAAAAACTCACCAAACGTTTTGAGAATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                                    <  1:3374041/139‑1 (MQ=255)

                                                                                      taagaCGTGATGAAAGCCCACAAATAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                                    <  2:248720/139‑1 (MQ=255)

                                                                                      caatccGTGTTTAAAGCCCACAAAGAAAATCTTACCAAACTTTTTTAGCATGAATAAAAAAGCGGCCGTAATGGCCGATTTTTTTAACCTTTGATTTCATAATTGTGATCACAGAAATGCTGCTTTACTATTTTGTGaaa                                                    <  1:4210183/135‑1 (MQ=255)

                                                                                      caagaCGTTATGAAAGCCCACCAACAAAAACTCACCAAACGATTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                                    <  1:3972693/139‑1 (MQ=255)

                                                                                        agaCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAATAAATGCGGCTTTACTATTTTGTGAAATa                                                  >  1:4979813/1‑140 (MQ=255)

                                                                                          aCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAgg                                                >  2:5169450/1‑140 (MQ=255)

                                                                                          aCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCGGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAgg                                                >  1:5113153/1‑140 (MQ=255)

                                                                                           cGTGATGCAACCCCACCACGACAAACTCACCAACCGTTTTGACCATGCTCCAAAAAGCGGCCGTAATGGCCGCTATTTTTAACCGGTGATTTCATCATGGTGATCAAAGAACTGCTGCTTTCCTATTTTGTGAACTAGGc                                               <  2:2681662/140‑1 (MQ=255)

                                                                                           cGTGATGAAAGCCCACCAACACACACTGACCAAACGTTTGGAGCATGCCTACAAAAGCGGCCGTCATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                                               <  2:1248478/140‑1 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAATCGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGGAATAGGcc                                              >  2:3559883/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCCTCATGGTGATCAAAGAAATGCTGGTTTCCTATTTTGTGAAATAgggc                                              >  1:3644489/1‑138 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGGTGCTTTCCTATTTTGTGAAATAGGcc                                              >  2:5358063/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                                         >  1:3048082/1‑129 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTTTGAAATAGGcc                                              >  2:4947113/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATATGcc                                              >  1:2032791/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                                              >  2:190066/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                                              >  2:1271129/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                                              >  2:2860481/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                                              >  2:2246727/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                                              >  2:914298/1‑140 (MQ=255)

                                                                                            gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCAGCTTTTTTTAACATGTGATTTCATCATGGGGATCAAATAAATGCTGATTTCCTATTTTGTGTGATAgggc                                              >  1:5225413/1‑138 (MQ=255)

                                                                                              gatgaAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTg                                            >  2:3837526/1‑140 (MQ=255)

                                                                                                tgaAAGCCAACCAAGAAAAACTCACCACACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGtt                                          <  2:5232939/140‑1 (MQ=255)

                                                                                                    aGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTAt                                      >  2:2092840/1‑140 (MQ=255)

                                                                                                    aGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCGGTTTTAt                                      >  1:3473560/1‑140 (MQ=255)

                                                                                                      cccACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgc                                    >  2:4216763/1‑140 (MQ=255)

                                                                                                          ataacaaaaaGTTACCAATTTTTTTTAGAATGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTTAAATATTACTGTTTTATgcagca                                <  1:4705573/135‑1 (MQ=255)

                                                                                                                 aaaCTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGGGAAATAGGCCGGTTTTATGCAGCAGCTCCtt                         >  2:937472/1‑140 (MQ=255)

                                                                                                                     tGACCACACGTTTTGAGCACGCACAAAAACGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGc                     <  1:1927197/140‑1 (MQ=255)

                                                                                                                     tGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGc                     >  2:3013220/1‑140 (MQ=255)

                                                                                                                     tGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTACTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGc                     >  2:1576965/1‑140 (MQ=255)

                                                                                                                      gACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                                    >  1:3144024/1‑108 (MQ=255)

                                                                                                                      gACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                                    <  2:3144024/108‑1 (MQ=255)

                                                                                                                             ctttttGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTg             <  1:2638273/138‑1 (MQ=255)

                                                                                                                             cGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTTCGATTg             <  1:5328015/140‑1 (MQ=255)

                                                                                                                             cGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTg             <  2:171545/140‑1 (MQ=255)

                                                                                                                             cGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTg             <  1:4624334/140‑1 (MQ=255)

                                                                                                                             cGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTg             <  2:755046/140‑1 (MQ=255)

                                                                                                                             cGTTTTGAGCATGAATAAAAAAGCGGCCGTAATGTCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTg             <  2:3382420/140‑1 (MQ=255)

                                                                                                                                  tGAGCATGCATAAAAACGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTGCAGTc        <  1:3118883/140‑1 (MQ=255)

                                                                                                                                     gcatgcatAAAAACGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTGCAGTCCTa     <  1:922696/140‑1 (MQ=255)

                                                                                                                                     gcatgcatAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTGCAGTCCTa     <  2:2393113/140‑1 (MQ=255)

                                                                                                                                     ccattcatAAAAAAGCGGCCGTAATGGCAGCTTTTTTTAACCGGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTGCAGTCCTa     <  2:343814/139‑1 (MQ=255)

                                                                                                                                       atgcatAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGGGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTGCAGTCCTACa   <  2:1023654/140‑1 (MQ=255)

                                                                                                                                        tgcatAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTGCAGTCCTACaa  >  1:4307969/1‑140 (MQ=255)

CTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCTGCGATTGCAGTCCTACAA  >  NZ_CP034943/3362275‑3362550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`