BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,394,434	T→G	6.6%	S156R (AGC→CGC)	ppaX ←	pyrophosphatase PpaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,394,434	0	T	G	6.6%	886.5 / 4.6	339	S156R (AGC→CGC)	ppaX	pyrophosphatase PpaX
Reads supporting (aligned to +/- strand): ref base T (115/180); new base G (38/3); total (156/183)
Fisher's exact test for biased strand distribution p-value = 1.70e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

ATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTATCTCTTAAT  >  NZ_CP034943/3394297‑3394570

ataATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCACCGGCTCGCTTc                                                                                                                                        <  2:3691922/140‑1 (MQ=255)

    tCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCATCACATCGGGGTAATTATCTCCGACCATAATTGCTTCCGCCGGCTCCCTTCCGAg                                                                                                                                    >  2:4289829/1‑140 (MQ=255)

     cAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGc                                                                                                                                   <  1:4917998/140‑1 (MQ=255)

        gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTTGGGCGGTTTTTCCTGGCAGCACATCGTGGTTATTATATACGACCATAATGGGTTGCGCCGGCTCGCGTGATAGCTGt                                                                                                                                >  1:3724401/1‑140 (MQ=255)

        gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGGGGCGGTTTTGCCTGCCCCCACATCGTGGTAATTATATGCCACCATAAAGGGTTCCGCCGGCTCGCTTCCTAGCTGt                                                                                                                                >  1:5170042/1‑140 (MQ=255)

        gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGGGTTTGTTGCGGCGGGTTTTCCAGCCCGCCCCTGGTGGTCAATATATCCCACCAAAAATGCTAGCGCCGGCGCGCGTCCtcggggg                                                                                                                                >  1:399640/1‑134 (MQ=255)

             tGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCGGTTTTGCCGGCCCGCACAGCGGGGTGATTATATACGACCAAAATTGGTTCCGCCGGCTCGCTTCCTAGCGGtgtgag                                                                                                                           >  1:4758700/1‑135 (MQ=255)

              gCTTTGCCAGTGTTTCCTGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAgc                                                                                                                          <  2:1758264/140‑1 (MQ=255)

                  tGCCCGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTACGACCATAATGGCTTCCGCCGGCTCGCTTACTAGCTGTTTCATCGCTa                                                                                                                      >  2:4542405/1‑140 (MQ=255)

                  tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGGCGGTTTTGTTCCGGCGTTTTTCCCGGCCAGCACATCGTGGTAATTATCACCGACCCAAAAGGCGTCCGCCGCCGCGCGTCCGAGCTGTTTCAccgcaa                                                                                                                      >  2:1382992/1‑138 (MQ=255)

                  tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTGCGGCGGTTTTGCCAGCCACCCCAACGTGGTAAATATATACGACCAAAAATGCGTGCGCCCGCTCGCGTGCGAGCAGTTTCatcccga                                                                                                                      >  1:3624828/1‑134 (MQ=255)

                  tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCCGCGGTTTTACATGGCAGCACATCGTGGTAATTATAAACCACCATAAAGGCTTCCGCCCGCGCGCGTACAAGCACTTTAAgcgcga                                                                                                                      >  2:2069556/1‑138 (MQ=255)

                  tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTGTGCGGCGTTTTTGGCGGGCCGCACAACGTGGGAATTTTATCCCGCCAGAATGGCTTCCGCCGGCGCGCGTGCTAGCGGTTTAAgcgcga                                                                                                                      >  1:2650539/1‑138 (MQ=255)

                   gacAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                                     <  1:1295595/138‑1 (MQ=255)

                   gCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                                     <  1:2570226/140‑1 (MQ=255)

                       gtgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGc                                                                                                                 <  1:5521170/140‑1 (MQ=255)

                        tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCCCGACATCGTGGTAAATAACTCCGACCAAAATGGCTTCCGCCGCCTCGCTTCCTAGCTGTGTCAGCGCTAAAAAca                                                                                                                >  2:326906/1‑140 (MQ=255)

                        tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                                <  1:2477697/140‑1 (MQ=255)

                        tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                                <  2:3145846/140‑1 (MQ=255)

                        tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGGGGTTTTGCCGGCCCCCCCAACGTGGGTATTTTATCCGACCAAAATGGCTTGCCCCGGCGCGCGTCGGATCGGTTTGAGAGCGAAAAgaa                                                                                                                >  1:5036839/1‑138 (MQ=255)

                        ggtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                                <  2:2525493/139‑1 (MQ=255)

                            tCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTACCGGCCCGCACATCGTGGTAATTATATACGACCAAAATTGCTTACGCCGGCTCGCGTACAAGCTGTGTGAGCGCTAAAAGcacacg                                                                                                            >  2:481322/1‑138 (MQ=255)

                              cGGCCCTTTACTCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGGACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                                          <  2:5199379/140‑1 (MQ=255)

                              cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGACTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                                          <  2:1627755/140‑1 (MQ=255)

                              cGGCCCGTTAATCGTCCATGCGACTCCGGCCGTTGTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATGAGCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                                          <  1:3953933/140‑1 (MQ=255)

                                    tttAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTCCCTGCCAGCACCTCGTGGTAAATATCTCCGACCATAATGGTTTGCGCCGGCTCGCTTCCTAGCTGTTTAATCGCTAAAAGCACCCGCTCGCGAt                                                                                                    >  2:3111290/1‑140 (MQ=255)

                                    tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                                                                                                    <  1:661703/140‑1 (MQ=255)

                                    tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                                                                                                    <  2:4063011/140‑1 (MQ=255)

                                    tttAATCGTCCATGCGACTCCGGCCGTGTTTGTTCCGGCGTTTTTGCCTGCCAGCACATCGTGGTAAGTATCACCGCCCAAAATGGCTTCCGCCGGCTCGCGTCCGAGCTGTTTCAGCGCTAAAACCACAGGCTAGGGAt                                                                                                    >  2:1323654/1‑140 (MQ=255)

                                      tAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGGGTTTTTGCCTGCCAGCACAACGTGGTAATTTACTCCGACCATAATTGCTTCCGCCCGCTCGCGTCCGAGCTGTTTGAGCGCTAAAAGCACACGCTCGGGATCa                                                                                                  >  2:921024/1‑140 (MQ=255)

                                      tAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCCTTTTTACCTGCCACCACATAGGGGTAATTATATACGACCATAATGGCTTCCGCCGGCTCGCTTCCAAGCTGTTTCAGCGCGAAAAGCACAGGCTCGGGCTCa                                                                                                  >  2:698910/1‑140 (MQ=255)

                                             ccATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTACAAGCACAGGCTCGGGATCAGGCTTTg                                                                                           <  2:2715097/140‑1 (MQ=255)

                                             ccATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCAGAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTg                                                                                           <  2:2745280/140‑1 (MQ=255)

                                              cATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTGCCTGCCAGCACATCGTGGTAATTATGTCCGACCATAATTGCTTCCGCCGGCGCGCTTGCTAGCTGTTTAAGCGCTAAAATCACAGGCTCGGGATCATGCTTTGc                                                                                          >  2:2843216/1‑140 (MQ=255)

                                              cATGCGACTCCGGCCGTGTTTGTTCCGGCGATTTTCCCTGCCCGCCCATAGTGGTAATTATCTCCGCCCAAAAAGGCTTCCGCCGGCTCCCGTCCTAGCGGTTTCAGCGCGAAAATCACAGGCTCGGGATCAGGCGTTGc                                                                                          >  2:401759/1‑140 (MQ=255)

                                               aTGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGcc                                                                                         <  2:3735438/140‑1 (MQ=255)

                                                tGCGACTCCGGCCGTTTTTGTTCCGGCGGGTTTGCCTGCCAGCACATCGGGGTAATTATCTCCGCCCATAATGGCTTGCGCCGGGTCGCTTGCTAGCTGTTTGAGCGCGAAAAGCACAGGCGCGGGATCAGGCGTTGGCa                                                                                        >  2:1048705/1‑140 (MQ=255)

                                                tGCGACTCCGGCCGTGTTTGTTCCGGGGTTTTTCCCGGCCACCCCCACGGGGTAATTATATCCCAACAAAATTGCGTGCGCCCGCGCGCGTCCTAGCTGTTTCAGCGCTAAAATCACAGGGTCGGGATCAGGCTTTGCCa                                                                                        >  2:856758/1‑140 (MQ=255)

                                                       ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                                 <  1:2153309/140‑1 (MQ=255)

                                                       ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                                 <  1:3371030/140‑1 (MQ=255)

                                                        cGGCCGTTTTTGTTCCGGCGTTTTTGCCTGCCCGCACAACGGGGTAATTATATCCGACCATAATTGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAACACAGGCTCGGGCTCAGGCTTTGCCAACGTcaca                                                                                >  2:1014184/1‑140 (MQ=255)

                                                             gTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACatcatc                                                                           <  2:387749/140‑1 (MQ=255)

                                                              tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  1:1535270/140‑1 (MQ=255)

                                                              tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  2:4474443/140‑1 (MQ=255)

                                                              tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  2:555618/140‑1 (MQ=255)

                                                              tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  1:3430264/140‑1 (MQ=255)

                                                              tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  2:3790493/140‑1 (MQ=255)

                                                              tttttGTTCCGGCGTTTTTCCCTGCCAGCACATATTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  2:2190819/140‑1 (MQ=255)

                                                              tttttGGTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGTCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGTTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  1:1003842/140‑1 (MQ=255)

                                                              tttgtGGTCCGGCGTTTTTCCCTGCCAGCCCATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAACGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  1:3080279/140‑1 (MQ=255)

                                                              gttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATACTGGCGTCCGCCGGCTCGCTTCCGAGCTGTTTCAGCGCTAAACGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                          <  1:5737620/139‑1 (MQ=255)

                                                                         gCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGAc                                                               >  2:5278033/1‑140 (MQ=255)

                                                                                             aTCGTGGTAATTATCTCCGACCATAAGGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGa                                           <  2:2609550/140‑1 (MQ=255)

                                                                                               cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGAtt                                         >  2:3588615/1‑140 (MQ=255)

                                                                                               cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGAtt                                         >  2:3527760/1‑140 (MQ=255)

                                                                                               cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGAtt                                         >  1:2352264/1‑140 (MQ=255)

                                                                                               cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGAtt                                         >  2:3159223/1‑140 (MQ=255)

                                                                                                gTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCGGTTGCAGCGCTAAAAGCACAGGCTCGGGCTCAGGCTTTGCCATCGTCACATCATCCAGGGTCACGCCCGGTTCAAAGTCTGCGCCGATTc                                        >  1:4222318/1‑140 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    >  1:1037944/1‑140 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    >  1:1389494/1‑140 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    <  1:4642789/140‑1 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    <  1:1746870/140‑1 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    <  1:1647957/140‑1 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    <  2:3016298/140‑1 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    <  1:3216682/140‑1 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    <  2:1937909/140‑1 (MQ=255)

                                                                                                    tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                    >  1:930174/1‑140 (MQ=255)

                                                                                                     aaTTATCTGCGACAATAATGGCTTCCGCCGGCTCTCTTCCTAGCTGTTTCAGCGCTAATTCCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTc                                   <  2:5668442/140‑1 (MQ=255)

                                                                                                     aaTTATCTCCGAACATACTGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTc                                   <  2:4168172/140‑1 (MQ=255)

                                                                                                       ttATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTCACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAg                                 >  2:1524674/1‑140 (MQ=255)

                                                                                                          tctcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCtt                              <  1:2560356/140‑1 (MQ=255)

                                                                                                            tcCGCCCATGCTGGCTGCCGCGGGCTGGCTTCCGCGCTGTTCCAGCGCCACAAGCACCGGCTCGGGATCAGGCTTTGCCATCGGCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGCGTCCCGTCAGCTGTa                            <  2:4726228/140‑1 (MQ=255)

                                                                                                            tcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                                     <  2:5768414/51‑1 (MQ=255)

                                                                                                            tcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTa                            >  1:4118606/1‑140 (MQ=255)

                                                                                                              cGACCATCATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTACAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg                          <  1:4802495/140‑1 (MQ=255)

                                                                                                              cGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTCgg                          >  1:749599/1‑140 (MQ=255)

                                                                                                              cGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg                          <  2:5656359/140‑1 (MQ=255)

                                                                                                              cGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg                          <  1:4874930/140‑1 (MQ=255)

                                                                                                              cGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg                          >  2:3380435/1‑140 (MQ=255)

                                                                                                              cGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg                          <  2:3297549/140‑1 (MQ=255)

                                                                                                                aCCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGcc                        <  2:3967829/140‑1 (MQ=255)

                                                                                                                aCCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGcc                        <  2:807383/140‑1 (MQ=255)

                                                                                                                aCCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAACTTTAGGcc                        <  1:2050005/140‑1 (MQ=255)

                                                                                                                aCCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGCCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGcc                        >  2:342730/1‑140 (MQ=255)

                                                                                                                    tAATGGCTTCCGCCGGCTCGCTTCTTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGa                                                                >  1:5238648/1‑96 (MQ=255)

                                                                                                                     aaTGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCatat                   >  1:3109189/1‑140 (MQ=255)

                                                                                                                     aaTGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCatat                   <  1:2285796/140‑1 (MQ=255)

                                                                                                                      aTGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATAtt                  <  1:5329955/140‑1 (MQ=255)

                                                                                                                      aTGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATAtt                  <  1:2172921/140‑1 (MQ=255)

                                                                                                                       tGGCTTCCGCCGTCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCAAATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTg                 <  2:5076752/140‑1 (MQ=255)

                                                                                                                        ggCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGa                <  2:621945/140‑1 (MQ=255)

                                                                                                                          cTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACa              <  1:3155831/140‑1 (MQ=255)

                                                                                                                            tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt            >  1:4333884/1‑140 (MQ=255)

                                                                                                                            tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt            >  1:2833175/1‑140 (MQ=255)

                                                                                                                            tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt            >  2:3064961/1‑140 (MQ=255)

                                                                                                                              ctccgGCTCGCTTCCTAGCTGTTTCATCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCTTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTAt          <  2:5394087/138‑1 (MQ=255)

                                                                                                                              cgccgGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTAt          <  2:2792208/140‑1 (MQ=255)

                                                                                                                               gccgGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTAtc         >  2:1136314/1‑140 (MQ=255)

                                                                                                                                   gCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTATCTCtt     >  1:892601/1‑140 (MQ=255)

                                                                                                                                      cGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTATCTCTTAAt  <  1:3559677/140‑1 (MQ=255)

ATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTATCTCTTAAT  >  NZ_CP034943/3394297‑3394570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`