Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,955 T→G 5.8% N211H (AAC→CAC)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9550TG 5.8% 1122.8 / 12.8 428N211H (AAC→CAC) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base T (191/194);  new base G (40/2);  total (232/196)
Fisher's exact test for biased strand distribution p-value = 1.14e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 448 total aligned reads displayed.

TATTGCTTTATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAAT  >  NZ_CP034943/3446816‑3447093
                                                                                                                                           |                                                                                                                                           
 aTTGCTTTATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGttt                                                                                                                                            >  1:4217439/1‑140 (MQ=255)
       ttATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAgatta                                                                                                                                      >  1:4496886/1‑139 (MQ=255)
       ttATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAcaata                                                                                                                                      >  1:2440942/1‑137 (MQ=255)
                 aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                           <  2:1568273/140‑1 (MQ=255)
                 aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                           <  2:1410435/140‑1 (MQ=255)
                 aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                           <  2:2936166/140‑1 (MQ=255)
                 aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                           <  1:1645466/140‑1 (MQ=255)
                      aaGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAata                                                                                                                      <  1:5159301/140‑1 (MQ=255)
                       aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTAATTTTTTGCAAAaaat                                                                                                                     >  1:2093094/1‑140 (MQ=255)
                       aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAatat                                                                                                                     <  2:5763068/140‑1 (MQ=255)
                       aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAatat                                                                                                                     <  2:433077/140‑1 (MQ=255)
                       aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTCAATTAATTTTTTGCAAAatat                                                                                                                     >  2:4421401/1‑140 (MQ=255)
                       aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCGCGTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAatat                                                                                                                     >  1:4608268/1‑140 (MQ=255)
                       aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGCAAAGTCTCTTATTATAATATCTCATTTCTACCAAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGAGCATTAATTTTTTTAAAAaaat                                                                                                                     >  2:902615/1‑140 (MQ=255)
                        gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCACGTCTCGTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAtt                                                                                                                    >  1:1244273/1‑140 (MQ=255)
                        gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTCATTTTTTTGAAAATAtt                                                                                                                    >  1:3281564/1‑140 (MQ=255)
                        gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAtt                                                                                                                    <  2:2721935/140‑1 (MQ=255)
                        gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAtt                                                                                                                    >  1:3116191/1‑140 (MQ=255)
                        gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAtt                                                                                                                    >  1:2932078/1‑140 (MQ=255)
                        gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAAtt                                                                                                                    >  1:5530184/1‑140 (MQ=255)
                          gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGCaaaaaaattc                                                                                                                  >  2:1689735/1‑134 (MQ=255)
                          gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTGCAAAATATTGc                                                                                                                  >  2:5483702/1‑140 (MQ=255)
                            aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATAATgata                                                                                                                >  1:3430526/1‑137 (MQ=255)
                            aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTc                                                                                                                >  2:301174/1‑140 (MQ=255)
                            aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTc                                                                                                                >  2:802253/1‑140 (MQ=255)
                            aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAGTTTTTGCAAAATATTGGTc                                                                                                                >  2:613921/1‑140 (MQ=255)
                            aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGGAAAATATTGCtg                                                                                                                >  1:4958902/1‑139 (MQ=255)
                             aCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATAGTTCTca                                                                                                               >  2:3650177/1‑140 (MQ=255)
                             aCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTGATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTca                                                                                                               >  2:5615997/1‑140 (MQ=255)
                               ggCCGCCGAAAAAAAGACCTCCCCAGTAAAAGGCAAGTCTATTATTAGACGATCTCAATTCTACCCAGCCATTTTTACTGGCGACCACAACGCCTTGCGTCCGGGCGTTTACATTCCTGTGTTGCAAAATATTGCTcaca                                                                                                             <  2:877672/140‑1 (MQ=255)
                               ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                             <  1:2028154/140‑1 (MQ=255)
                               ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                             >  2:2641823/1‑140 (MQ=255)
                                 cAGCCTAACAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTCCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATg                                                                                                           <  2:3053348/140‑1 (MQ=255)
                                    ccTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGtt                                                                                                        >  2:2036981/1‑140 (MQ=255)
                                          aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                  <  1:1422167/140‑1 (MQ=255)
                                          aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                  <  1:5732507/140‑1 (MQ=255)
                                          aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                  <  2:414819/140‑1 (MQ=255)
                                              gACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCGTGCGTCCGGGCGTTTACATTCAATTTTTTCAAAATAATTCTCAAAAGGTTATTAACGCtt                                                                                              >  2:1942113/1‑140 (MQ=255)
                                                  tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                          <  2:2545059/140‑1 (MQ=255)
                                                   gCCTAGTAAAAGGCAAGTCTCCTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGTAAAATATTGCTGACATGGTTTTTAACGGTTTtctc                                                                                         <  1:1837020/140‑1 (MQ=255)
                                                         tAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCAAATGGTTTTTGACGGTTTTCTGACTgtt                                                                                   >  2:4961735/1‑138 (MQ=255)
                                                          aaaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                  <  2:2187531/140‑1 (MQ=255)
                                                          aaaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                  <  2:5138752/140‑1 (MQ=255)
                                                             aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                               <  2:3195045/140‑1 (MQ=255)
                                                             aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                               <  2:2440942/140‑1 (MQ=255)
                                                             aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                               <  2:3895268/140‑1 (MQ=255)
                                                             aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATGTTTAATTGCGACCACAACGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                               <  1:488207/140‑1 (MQ=255)
                                                              ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAATTGATTTTTTTGAAAATATTTCTCACAAGGTTTTTGACGGTTTTGTCACTGATaaaaa                                                                              >  1:3596278/1‑138 (MQ=255)
                                                               gCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACaa                                                                             <  2:3717158/140‑1 (MQ=255)
                                                                  aGTCTCTTATTATACTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTAGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                          <  1:960671/140‑1 (MQ=255)
                                                                  aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                          <  1:829129/140‑1 (MQ=255)
                                                                  aGTCTCTTAGTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACCACGGCTTGCGTCCGGTCGCTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                          <  2:4390879/140‑1 (MQ=255)
                                                                   gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                         >  2:4041122/1‑140 (MQ=255)
                                                                   gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAATTTTTTTCAAAATATTTGTGAGAGGGTTTTTCACCGTTTTGTCACTGATAAAAAAttct                                                                         >  1:4684144/1‑137 (MQ=255)
                                                                       ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAc                                                                     <  2:4254836/140‑1 (MQ=255)
                                                                       ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGATTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAc                                                                     <  1:1148083/140‑1 (MQ=255)
                                                                              taCTATCTCATTTCTACCCAGCCATTTTTAATGCCGACCACAACGGCTTGCGTCCGGGCGTTTACATTCAATGTTTGCAAAATATTGGTCCCATGCGTTTTCACGGTGTTCGCACGGAGTAACCACGAGTCACCAATAcc                                                              >  2:4504255/1‑140 (MQ=255)
                                                                                  aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTTCAAAATATTGGTCACATGGTTTTTAACGGTTTTATCACTGATAAAAAAATATTCAACCAAACCCCgg                                                          >  1:153322/1‑140 (MQ=255)
                                                                                  aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATAAATTTTTTCAAAAAATTGCTCACAAGGTTTTTGAACGTTTTATCACAGATAAAAAAAGATTCAACAAAAAcgccg                                                          >  1:1716808/1‑138 (MQ=255)
                                                                                  aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTATACATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTATCGCTTTTGTCACTGATAAACAATGATTCACCCATAACGCgg                                                          >  2:1772112/1‑140 (MQ=255)
                                                                                   tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACCTTCATTTTTTGCAAAATATTGCTCACAATGTTTTTAACGGTTTTGTCACTGATAAAAACAGATTAACCAATACCTCGGt                                                         >  2:3363198/1‑140 (MQ=255)
                                                                                   tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACAAGGTTTTTCACGGTTTTCTCACTTATAAAAAAAGATTAAACCAAACCGCGGt                                                         >  2:4686212/1‑140 (MQ=255)
                                                                                   tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAATTGCTCACATGGTTTTTCACGGTTTTATAACTGATAAACAATGATTAACCAATAACGCGGt                                                         >  2:1173238/1‑140 (MQ=255)
                                                                                   tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAAAAATGCTAACAAGGTTTTTAACCGTTTTATCACTTATAAACAAAAATTCACCAATACCCCGGt                                                         >  1:5368862/1‑140 (MQ=255)
                                                                                   tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCATTCAGTTTTTTCCAAATATTGCTCACATTGTTTTTGACCGTGTTGTAACAGAGAAACAAAGTTTCACCAAAAACGAGGt                                                         >  1:102798/1‑140 (MQ=255)
                                                                                    ctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCAAAATATTTCACACATGGTTTTTCAAGCTTTTCTCACTTAAAAAAAATGATTGACCAAAAACGCgggt                                                        >  1:4316917/1‑138 (MQ=255)
                                                                                     tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCAATGATAAAAAATGATTCACCAATACCGCGGTTg                                                       >  2:1081720/1‑140 (MQ=255)
                                                                                        tttCTAGCCAGCCCTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTGAATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACCGTTTTATCAATTAAAAAAAAAGAATAACCAATAACGCGGTTTCtt                                                    >  2:2344981/1‑140 (MQ=255)
                                                                                        tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTAACCGGTTTATCACTGATAAACAATGATTCACCCATAACCCCGTTGCtt                                                    >  1:3879465/1‑140 (MQ=255)
                                                                                        tttCTACCCAGCCATTTTTAATGGCGACCACAAAGGCTTGCGTCCCGTCGTTTACATTCCTTTTTTGCCAAATAGTGAGAACATGGTTTATCACGGTTTTCACACTGATTAACAATGATTAAGCCATACCGCGCTTGGtt                                                    >  2:4739009/1‑140 (MQ=255)
                                                                                             aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                               <  1:4127896/140‑1 (MQ=255)
                                                                                                 aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTGATTTTTTTGAAAATATTTTTGAAATTGTTTTTAACGGTTTTATCACTGATAAAAAATTATTGACCAATAACGCGGTTTGTTTTTGGGTCt                                           >  1:983684/1‑140 (MQ=255)
                                                                                                 aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                           >  1:270627/1‑60 (MQ=255)
                                                                                                   ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTAACCGTTTTGTAACTGATAAACAAAGATTAAACAAAAACGCGGTTGCTTTTTACGTCTGc                                         >  2:1488749/1‑140 (MQ=255)
                                                                                                      tttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAAAAATGATTAAACAATACCGCGGTTGCTTTTTACGTCTTCAAg                                      >  2:329917/1‑140 (MQ=255)
                                                                                                       ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                     <  1:880421/140‑1 (MQ=255)
                                                                                                              ggCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgca                              <  2:5242594/140‑1 (MQ=255)
                                                                                                               gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgca                              <  2:1296298/140‑1 (MQ=255)
                                                                                                               gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                             <  2:4850800/140‑1 (MQ=255)
                                                                                                               gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                             <  1:3013577/140‑1 (MQ=255)
                                                                                                               gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCAAATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                             <  2:182741/140‑1 (MQ=255)
                                                                                                                cGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTGCAAAAATTTCTCACAAGGTTTTTGACGGTTTTATCACTGTTAAAAAAAGATTAACCAATAACGCGGTTGGTTTTTACGGCGGGCAGCAACTgcagc                            >  1:1497809/1‑140 (MQ=255)
                                                                                                                 gACCACCACGGCTGGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCAGATGGCTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcagca                           <  1:4421401/140‑1 (MQ=255)
                                                                                                                 gACCACACCGGCTTGCGTCCGGTCGTTTACCTTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATACACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                           <  1:2550503/140‑1 (MQ=255)
                                                                                                                 gACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTGAAAATATTTGTCACATTGTTTTTGACGGTTTTATCACTGATAAACAATGATTAAACAATAACGCGGGTGGTTTTTAAGTCTGCAATCAACTgcagga                           >  1:4849427/1‑138 (MQ=255)
                                                                                                                  aCCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCAc                          >  2:2847722/1‑140 (MQ=255)
                                                                                                                    cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACTATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                        <  1:558881/140‑1 (MQ=255)
                                                                                                                            cTTGCGTCCGGTCGTTTACATTCATTTTTTTGAAAAAATTGCTCACAAGGTTTTTAAAGGTTTTTTAACTGATAAACAATGATTCAACCAAAACGCGGTTGGTTTTTTCGTCTGCAAGCAACAGCAGCACGTaacaatac                >  1:524692/1‑136 (MQ=255)
                                                                                                                               gCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTGGTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTCACCAATACCGCGGTTTCTTTTTGCGTCTGCAATCATCTGCATCACTTCACATTCccgc             >  2:5677647/1‑140 (MQ=255)
                                                                                                                                 gTCCGGTCGTTTTCATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAAAAATTATTCACCAATACCGCGGTTGGTTTTTCCGTCTGCAAGCAACTGCAGCACTTCACATTAccgccg           >  2:4216941/1‑140 (MQ=255)
                                                                                                                                    cGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTACGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGt        >  2:1335227/1‑140 (MQ=255)
                                                                                                                                      gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa      <  1:1312164/140‑1 (MQ=255)
                                                                                                                                        cgattACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGAGAAACAATGATTCACCAAGACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa    <  1:3667553/137‑1 (MQ=255)
                                                                                                                                        cGTTTACATTCATTTTTTGCAAAATATTGCTCTCATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa    <  2:849265/140‑1 (MQ=255)
                                                                                                                                        cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa    <  1:667237/140‑1 (MQ=255)
                                                                                                                                        cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa    <  2:5537152/140‑1 (MQ=255)
                                                                                                                                        cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa    <  1:3169233/140‑1 (MQ=255)
                                                                                                                                        cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa    <  1:2169898/140‑1 (MQ=255)
                                                                                                                                        cGTTTAAATTCATTTTTTGCAAAATATTGCTCACATGTTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATATGCAGCACTTCACAGTCCCGCCGAGTTaaa    <  1:4006616/140‑1 (MQ=255)
                                                                                                                                          tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat  <  1:2364432/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TATTGCTTTATAACTCTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAAT  >  NZ_CP034943/3446816‑3447093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: