Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,995 C→A 5.1% V197V (GTG→GTT degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9950CA 5.1% 1304.5 / 10.1 490V197V (GTG→GTTdegUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base C (105/317);  new base A (44/3);  total (170/320)
Fisher's exact test for biased strand distribution p-value = 5.87e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 490 total aligned reads displayed.

AAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGA  >  NZ_CP034943/3446858‑3447133
                                                                                                                                          |                                                                                                                                          
aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                                          <  2:178936/140‑1 (MQ=255)
aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                                          <  1:2091855/140‑1 (MQ=255)
aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                                          <  1:769034/140‑1 (MQ=255)
aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                                          <  1:1393629/140‑1 (MQ=255)
 aaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACg                                                                                                                                         <  1:3865457/140‑1 (MQ=255)
        tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                                                  <  2:2545059/140‑1 (MQ=255)
         gCCTAGTAAAAGGCAAGTCTCCTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGTAAAATATTGCTGACATGGTTTTTAACGGTTTtctc                                                                                                                                 <  1:1837020/140‑1 (MQ=255)
          cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                                <  2:4797507/140‑1 (MQ=255)
          cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                                <  2:5705815/140‑1 (MQ=255)
             aGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCACCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTg                                                                                                                             <  2:802289/140‑1 (MQ=255)
                aaaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                                                          <  2:5138752/140‑1 (MQ=255)
                   aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                                                                       <  2:3195045/140‑1 (MQ=255)
                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAATTGATTTTTTTGAAAATATTTCTCACAAGGTTTTTGACGGTTTTGTCACTGATaaaaa                                                                                                                      >  1:3596278/1‑138 (MQ=255)
                     gCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACaa                                                                                                                     <  2:3717158/140‑1 (MQ=255)
                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                  <  1:3236812/140‑1 (MQ=255)
                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                  <  1:4213468/140‑1 (MQ=255)
                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                  <  2:1943485/140‑1 (MQ=255)
                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                  <  1:125245/140‑1 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                                                 >  2:4041122/1‑140 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAATTTTTTTCAAAATATTTGTGAGAGGGTTTTTCACCGTTTTGTCACTGATAAAAAAttct                                                                                                                 >  1:4684144/1‑137 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACCTTAATTTTTTGGAAAATATTGCTCACATGGTTTTTACCGGGTTTATGACTGATAAACACTGAt                                                                                                                 >  1:4881373/1‑140 (MQ=255)
                          tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                                <  1:2616364/140‑1 (MQ=255)
                              ttcttctaCTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACACCGGCTTGCGTCCGGGCGTTTACATTCATTTTTTGCAAAATATTGCACACATGGTTTTTCACGGTTTTCTCACTGATAAACCAAGATTCAcc                                                                                                            >  2:3013533/7‑140 (MQ=255)
                               tattatACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATAATTCTCCCAAGGTTTTTCAAGGTTTTATAACTGATAAACAAAGATTCACCa                                                                                                           >  1:153402/1‑140 (MQ=255)
                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTCTCACAAGGTTTTTAACGGTTTTATCACTGATAAACAATGATTCACCAATAcc                                                                                                      >  2:2206859/1‑140 (MQ=255)
                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATCATTTTTTTCAAAATATTTGTAACATGGTTTTTAAAGGTTTTATCAATGATAAAAAATGATTAACCAATAcc                                                                                                      >  1:967842/1‑140 (MQ=255)
                                      cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTCAAGGTTTTATAAATGATAAAAAAAGATTCAACAAAACcgc                                                                                                    >  2:325766/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTAACATTGTTTTTCAAGGTTTTCTCACTGATAAACAATGATTCACCAATAcagagg                                                                                                  >  2:5256447/1‑135 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTGACGGTTTTATCAATGATAAAAAAATATTCACCAAAACCGCgg                                                                                                  >  1:5803376/1‑140 (MQ=255)
                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCATTCAGTTTTTTCCAAATATTGCTCACATTGTTTTTGACCGTGTTGTAACAGAGAAACAAAGTTTCACCAAAAACGAGGt                                                                                                 >  1:102798/1‑140 (MQ=255)
                                         tctcAGTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCGCACATGGTTTTTACCGGGTTTCTCACTGATAAACACTGATTCACCAATAACGCggg                                                                                                 >  2:3573219/1‑139 (MQ=255)
                                          ctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTGATTTTTTGCAAAAAATTTCTCACAAGGTTTTTAACCGTTTTATAACTGATAAACAAAGAATAACCAAAACCCCGGtt                                                                                                >  1:2418315/1‑140 (MQ=255)
                                              tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTGTTTGCAAAATATTGCTCACAAGGTTGTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCtt                                                                                            >  2:1442365/1‑140 (MQ=255)
                                                 cTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTGCAAAAAATTTCTCACATGGTTTTTGACCGTTTTATAACTGATAAACAATGAGTAACCAATAACGCGGTTGCttttt                                                                                         >  2:1821166/1‑140 (MQ=255)
                                                    cccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAATTTTTTTAAAAAAATTGCTCACATGGTTTTTAAAGGTTTTCTCAATTATAAAAAATTATTCACCAATACCGCCGTTGCtttttaag                                                                                      >  2:667237/1‑137 (MQ=255)
                                                       aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACCTTCATTTTTTGCACAATATTGCGCAAATGGTTTTGCACGGGGTTCTCACTGATTAACCAAGATTCACCCATACCGCGGGTGCTTTTTCCGtgt                                                                                   >  2:5554307/1‑138 (MQ=255)
                                                       aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGTTTTTTGACGGTTTTGTCACTTATAAAAAATGATTAACCAATAACGCGGGTTCTTTTTACGTCt                                                                                   >  2:870790/1‑140 (MQ=255)
                                                       aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAATATTGCTCACCTGGTTTTTCACGGTTTTTTCACAGATAAACAAAGATTCACCAAAAACCCGGTTTCTTTTTACGTCt                                                                                   >  1:3161285/1‑140 (MQ=255)
                                                       aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAATATTGCTCACAGTGTTTTTAACGGTTTTATCACTGATAAACAATGATTAACCAATACCGCGGTTGCTTTTTcactct                                                                                   >  2:5039783/1‑135 (MQ=255)
                                                            tttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTGTCACTGATAAACAAAAATTAACCAATAACCCGGTTTCTTTTTACGTCTGCAAg                                                                              >  2:3310665/1‑140 (MQ=255)
                                                             ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                                             <  2:5617039/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgca                                                                      <  2:1296298/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  2:4314976/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  2:4850800/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  1:5284670/140‑1 (MQ=255)
                                                                       gACCACCACGGCTGGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCAGATGGCTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                                   <  1:4421401/140‑1 (MQ=255)
                                                                       gACCACAACGGCTTGCGTCCGGTCGTGTTCCTTGATTTTTTGGAAAAAATTGCTCACATTGTTTTTGACGGTTTTGTCACTGATAAACAATGATTGACCCATAACGCGGGTTGTTTTTGGGGGTGCAACCATCTggagca                                                                   >  1:5082052/1‑140 (MQ=255)
                                                                        aCCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTGACTGATAAAAAATTATTAAACAATAACGCGGTTTCTTTTTGCGTCTGCAAGCATCTGCAGCAc                                                                  >  2:4508814/1‑140 (MQ=255)
                                                                        aCCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCGCACCAGGGTTTTGACCGTTTTATCACAGATAAACAAATATTCACCAATTCCGCGGTTGCGTTTTCCGTGTGCAAGCATCTGGAGCAc                                                                  >  2:2296525/1‑140 (MQ=255)
                                                                        aCCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCAc                                                                  >  2:2847722/1‑140 (MQ=255)
                                                                          cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                                                <  2:204628/140‑1 (MQ=255)
                                                                                       gTCCGGTCGTTTTCATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAAAAATTATTCACCAATACCGCGGTTGGTTTTTCCGTCTGCAAGCAACTGCAGCACTTCACATTAccgccg                                                   >  2:4216941/1‑140 (MQ=255)
                                                                                          cGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATTGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTACGTCTGCAAGCATATGCAGCACTTCACATTACCCCCGAGt                                                >  2:1111169/1‑140 (MQ=255)
                                                                                          cGGTCGTTTACATTCATTTTTTGCAAAATAATGCTCACAAGGTTTTTCACGGTTTTATCACTGATAAAAAATGATTAACCAAAACCGCGGTTGCTTTTTACGTCTTCAACCATCTGCAGCACATCACATTGCCGCCGAGt                                                >  2:3849410/1‑140 (MQ=255)
                                                                                           ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                               <  1:1708150/140‑1 (MQ=255)
                                                                                           ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                               <  1:1485009/140‑1 (MQ=255)
                                                                                            gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                              <  1:4925251/140‑1 (MQ=255)
                                                                                              cttttACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  2:4397320/138‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCATAAGATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTACGTCTGCAAGCATCTGCAGCACTTCTCATTCCCGCCGAGTTaaa                                            <  1:3306314/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  2:2716254/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTaaa                                            <  1:1109836/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:436576/140‑1 (MQ=255)
                                                                                              cGTTTACAGTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:4546937/140‑1 (MQ=255)
                                                                                              cGTTTAAATTCATTTTTTGCAAAATATTGCTCACATGTTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATATGCAGCACTTCACAGTCCCGCCGAGTTaaa                                            <  1:4006616/140‑1 (MQ=255)
                                                                                                tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                          <  1:1514010/140‑1 (MQ=255)
                                                                                                tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                          <  1:2364432/140‑1 (MQ=255)
                                                                                                     attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                     <  2:4684144/140‑1 (MQ=255)
                                                                                                        cattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATg                                  <  1:5478802/140‑1 (MQ=255)
                                                                                                         attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                                 >  2:1371817/1‑140 (MQ=255)
                                                                                                         attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                                 >  1:4116853/1‑140 (MQ=255)
                                                                                                              tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg                             <  1:3701910/140‑1 (MQ=255)
                                                                                                                tGCAATATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  2:4124463/140‑1 (MQ=255)
                                                                                                                tGCAAAATATTGCTCACCTGGTTTTTCACGGTTGTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCGTTGTCCGTCTGCAAGCATCTGCAGCACTTCCCAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:2746141/140‑1 (MQ=255)
                                                                                                                tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:1172279/140‑1 (MQ=255)
                                                                                                                tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:1808638/140‑1 (MQ=255)
                                                                                                                tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:558838/140‑1 (MQ=255)
                                                                                                                tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  2:1204601/140‑1 (MQ=255)
                                                                                                                tGCAAAATAGTGATAACATGGTTTTTCACGTTTTTCTAACTGATAAACAATGATTCACCAATACCGCGGGTGATTTTTCCGTCTGCAAGAATCTGCAGCACTTCACATTCCCGCATAGTTAAAATATGTAATGGTCTGCg                           <  1:227093/140‑1 (MQ=255)
                                                                                                                  cAAAATATTGCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGa                         <  2:2510924/140‑1 (MQ=255)
                                                                                                                     aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                      <  1:891425/140‑1 (MQ=255)
                                                                                                                     aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                      <  1:849002/140‑1 (MQ=255)
                                                                                                                           gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  1:4807621/140‑1 (MQ=255)
                                                                                                                           gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  1:2942948/140‑1 (MQ=255)
                                                                                                                           gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  1:4253859/140‑1 (MQ=255)
                                                                                                                           gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  2:153402/140‑1 (MQ=255)
                                                                                                                             tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg              <  1:3050845/140‑1 (MQ=255)
                                                                                                                                 ctGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  2:5803376/139‑1 (MQ=255)
                                                                                                                                 attgTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:2211173/137‑1 (MQ=255)
                                                                                                                                 aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:5677647/140‑1 (MQ=255)
                                                                                                                                 aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:1335227/140‑1 (MQ=255)
                                                                                                                                 aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:2483756/140‑1 (MQ=255)
                                                                                                                                 aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:5191303/140‑1 (MQ=255)
                                                                                                                                 aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:3445032/1‑140 (MQ=255)
                                                                                                                                 aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  2:5527744/140‑1 (MQ=255)
                                                                                                                                 aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:2028259/1‑140 (MQ=255)
                                                                                                                                 aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCGGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  2:3430526/140‑1 (MQ=255)
                                                                                                                                      ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGc     <  2:538110/140‑1 (MQ=255)
                                                                                                                                        ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg   <  2:1073986/140‑1 (MQ=255)
                                                                                                                                        ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg   <  2:3072702/140‑1 (MQ=255)
                                                                                                                                        ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg   <  2:1660279/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGA  >  NZ_CP034943/3446858‑3447133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: