Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,496,387 G→T 8.8% S347R (AGC→AGA EO946_RS17995 ← glucosaminidase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,496,3870GT 8.8% 823.5 / 17.1 317S347R (AGC→AGAEO946_RS17995glucosaminidase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (68/211);  new base T (36/2);  total (104/213)
Fisher's exact test for biased strand distribution p-value = 1.23e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 322 total aligned reads displayed.

CTGCTGTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATA  >  NZ_CP034943/3496248‑3496517
                                                                                                                                           |                                                                                                                                   
ctgctgTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGGTg                                                                                                                                     >  2:1018529/1‑140 (MQ=255)
ctgctgTAGTTCCTGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTCGACATGGTCGTTTTTTTGATg                                                                                                                                     <  1:5417293/140‑1 (MQ=255)
     gttgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGa                                                                                                                                <  2:2340682/140‑1 (MQ=255)
       tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                              <  2:604721/140‑1 (MQ=255)
       tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                              <  1:1539626/140‑1 (MQ=255)
       tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                              <  2:5754533/140‑1 (MQ=255)
       tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                              <  1:3280404/140‑1 (MQ=255)
             aGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATTACgtgt                                                                                                                        >  2:2019448/1‑140 (MQ=255)
                tGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAt                                                                                                                    <  2:5212038/140‑1 (MQ=255)
                                  tACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaa                                                                                                  <  1:4966358/140‑1 (MQ=255)
                                     ttttttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaaga                                                                                               <  2:3678887/140‑1 (MQ=255)
                                     ttttttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaaga                                                                                               <  2:5117823/140‑1 (MQ=255)
                                      tttttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaagaa                                                                                              <  1:4788085/140‑1 (MQ=255)
                                      tttttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaagaa                                                                                              <  1:1415595/140‑1 (MQ=255)
                                         ttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATTCTTGATGACGTGTGATGGCATTTTTTGTTTTtaaataaaat                                                                                           >  1:4663036/1‑138 (MQ=255)
                                         ttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATTTCGTGTAATTCCATTTTTTATTTTTAAATAAAgt                                                                                           >  2:4362415/1‑140 (MQ=255)
                                                   agaagGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCttt                                                                                 <  2:3432670/140‑1 (MQ=255)
                                                   agaagGCTGCGGCTTTGTGTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTCTTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCttt                                                                                 <  2:1092814/140‑1 (MQ=255)
                                                          tGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTGTTTTTGTTGCTGGCTGAGGTGGAATGACAATTTTTCTTTTTTAAGTAATTGTATATCTTTTTGAgct                                                                          >  1:5167192/1‑140 (MQ=255)
                                                           gCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAgctg                                                                         <  1:1344374/140‑1 (MQ=255)
                                                             ggCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAgctgct                                                                       <  2:5574610/140‑1 (MQ=255)
                                                                 ttgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACCTGGTCGTTTTTTTGATGCTGGATGACGGGTAATGCCATTTTTTCTTTTTAAATAAAGTGTCTATAGTTTGCAGCTGCTGttt                                                                   >  2:2664207/1‑140 (MQ=255)
                                                                 ttgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATTACGTGTAATGCCATTTTTTGTTTTTAAAGAAAGTTGGTATCTTTTTCAGCTGCTGgtt                                                                   >  2:3734012/1‑140 (MQ=255)
                                                                     ttgcttgcTGCTCGCCTAAAATCTGCGCCGTCGTTTTTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGtt                                                               <  2:4043122/140‑1 (MQ=255)
                                                                       gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                             <  2:1772237/140‑1 (MQ=255)
                                                                       gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                             <  2:5707043/140‑1 (MQ=255)
                                                                       gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                             <  2:3212077/140‑1 (MQ=255)
                                                                       gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                             <  2:5503138/140‑1 (MQ=255)
                                                                       gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATGGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                             <  1:2046754/140‑1 (MQ=255)
                                                                         ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTTTGCTGGATGACGTGTAATTGCCATTTTTCTTTTTAAATAAACTGTCTATCTTTTTGAGCTGCTGTTTAGTTAAcc                                                           >  2:851772/1‑140 (MQ=255)
                                                                         ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGTTGCTGGATTAAGTGTAATTCCATTTTTTATTTTTAAAGAAAATGTCGATATTTTTCAGCTGCTGTTTAGTTaaaa                                                           >  2:1812898/1‑138 (MQ=255)
                                                                         ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                           <  2:2158232/140‑1 (MQ=255)
                                                                         ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                           <  2:2198520/140‑1 (MQ=255)
                                                                         ttgctGCTCGACTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                           <  1:3089857/140‑1 (MQ=255)
                                                                           gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                         <  2:5768313/140‑1 (MQ=255)
                                                                            ctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATTCTGGATGACCTGTAATGCCATTTTTTATTTTTAAAAAAAGTGTATATCTTTTGCAGCTGCTGTTTAGTTAAaccca                                                        >  2:1227384/1‑139 (MQ=255)
                                                                             tgttCGCCTAAAATCTGCGCCGTCGTTATTTGATAGGCTGTAGAATTGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCtt                                                       <  2:2861914/137‑1 (MQ=255)
                                                                             tgctatccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCtt                                                       <  2:5153903/134‑1 (MQ=255)
                                                                              gctCTCATAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGAGATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTc                                                      <  2:4687573/140‑1 (MQ=255)
                                                                              gctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTc                                                      <  1:2664207/140‑1 (MQ=255)
                                                                              gctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTGTTTTTGATGCTGGATGACGTGTTATGCCATTTTTTGTTTTTAAATAAAGTGTCTATGTTTTGCGGCTGGTGGTGGGTTAAGCGCttg                                                      >  2:5586217/1‑139 (MQ=255)
                                                                                 cGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCata                                                   <  2:2100847/140‑1 (MQ=255)
                                                                                  gCCTAAAATCTGCTCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATTACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCatat                                                  <  1:3470203/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                 <  2:3828244/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                 <  1:4723928/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                 <  1:4267386/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                 <  2:4748182/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                 <  1:2757788/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                 <  2:2806312/140‑1 (MQ=255)
                                                                                   ccTAAAATCGGCGCCGTCGTTATTGGATAGTCTGTAGCCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                 <  1:5564749/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTGGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTTGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                <  1:4582931/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                <  2:4395639/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                <  2:3998922/140‑1 (MQ=255)
                                                                                     tAACATCTGCGCCGTCGTTATTGGATAGGCTGTAGACCTGGTCGTTTTTTTGATTCTGGATGACGTGTAATGCCATTGTTTCTATATAAAGAAAGTTTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTACATATTCa                                               <  1:2512297/140‑1 (MQ=255)
                                                                                     tAAATTCTGCGCCTTAGTTAGTGGTTCGGATGTAGACATGGTCGTTTTTTTGATGCTGGATTTCGTGTTATGGAATTTTTTCTTTTTACAGATAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACACATTCATATTCa                                               <  2:4274096/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                               <  1:10354/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                               <  2:5416432/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                               <  2:1579688/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                               <  1:703049/140‑1 (MQ=255)
                                                                                      aataTCTTCGGCGTCGTTTTTGGATAGGCTGTAGACTTGGTCGTTTTTTTGATTCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTTTCTATCTTTTGCAGCTGCTGTTTCTTTAACCCCTTCATATTCAc                                              <  2:5173019/137‑1 (MQ=255)
                                                                                      aaacTCTGCGCCGTCGTTATTGGAGAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATATTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:2196273/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCTTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  2:3347918/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  2:2560314/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:3369032/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:2792806/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:4011746/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:2295178/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:3710480/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:5752664/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:1909198/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  2:649526/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGAGAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                              <  1:282351/140‑1 (MQ=255)
                                                                                       aaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACt                                             <  2:2680027/140‑1 (MQ=255)
                                                                                       aaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACt                                             <  1:4299067/140‑1 (MQ=255)
                                                                                         agcTGCGCCGTCGTTATTGGCTAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTACTGCCATATTTTCTTTTTAACGAAAGTGTCTATCTTTTGCAGCTGCTGTGTCGTTAACCCCTTCATAGTCACTGt                                           <  2:3533306/138‑1 (MQ=255)
                                                                                              cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTGTGCTGGATGACGGGTAAATCCCTTTTTTCTTTTTAAAGAAAGTGTGTAAATTTTTCAGCTGGTGTTTGGTTAAACCCTTCATATTAAAAGtataat                                      >  2:1909198/1‑138 (MQ=255)
                                                                                              cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTATTCTGGGTGCGGTGTTATTGCATTTTTTTTTTTTTAAGTAAGTTGGTATCGTTTTCAGCTTCGGTGTGGTTAACCCCATGAAATTCACCGTATatg                                      >  1:1288048/1‑140 (MQ=255)
                                                                                              cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGTTTCTGGATGTCGGGTAATGGCATTTTTTGTTTTTAAAGTAAGTTTCTATCTTTTGAATCTGCTTTTTAGTTTAACACATAAAATTGACTGTatatt                                      >  2:2474095/1‑139 (MQ=255)
                                                                                                ccGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTTCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatgat                                    <  2:4997456/140‑1 (MQ=255)
                                                                                                    cgtTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGc                                <  1:5492659/140‑1 (MQ=255)
                                                                                                        aTTGGATAGGCTGTAGACATGGTCGTTTTTTTTTTTGTTGATGACGTGTAAAGCCATTTTTTGTTTTTAAAGAAAGTTTGGATAGTTTTCATCTGCGTTTTAGTTAAACCCTTAAGATTGACAGTATATGATTTGCtttt                            >  2:3127480/1‑140 (MQ=255)
                                                                                                           ggATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTc                         <  1:781632/140‑1 (MQ=255)
                                                                                                           ggATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTc                         <  1:565234/140‑1 (MQ=255)
                                                                                                            gATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGGATATGATGTGCTTTTGTCa                        <  2:305108/140‑1 (MQ=255)
                                                                                                                 gCTGTAGACATGGTCGTTTTTTTTTTGCTGGCTGACGTGTAATGCCATTTTTTGTTTTTTAAGAAAGGTTGGATGTGTTGGAGCTGCTGGTTCGTTTAACCCTTGATATTACCTGGTTATTATTTGCTGTTGTGaaacgg                   >  1:4141950/1‑136 (MQ=255)
                                                                                                                 gCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCCTTTTTTGTTTTTAAAGAAAGTTTCTATCGTTTTCAGCTGGTGTTTAGTTAAACCGGTAAAATTAACTGGATATTATGTTGGTTTGTCAAACTg                   >  2:5289699/1‑140 (MQ=255)
                                                                                                                   tGTAGACATGGTCGTTTTTTTGATGGTGGAAGAAGTGTAATTCCATTTTTTCTTTTTAAACAAATTGTCTAAGTTTTGCAGCATCTGTTTCGTTAAAACCGTCATATTGACAGTTTAAGATTTTCTGTTGTaaaacattt                 >  2:1983522/1‑134 (MQ=255)
                                                                                                                     tAGACATGGTCGTTTTTTTGAAGCTGGATGACGTGTAATGCCATTTTTTTTTTTTAAAGAAAGTGTCTAGCTTTTTCAGCTGCTGTTTAGTTAACCCCTTCATATTAAATTTATATGTTTTTCTTTTTTCAACCTGttta               >  2:1441503/1‑138 (MQ=255)
                                                                                                                      aGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAAGCCAATTTTTCTTTTTAAAGAAAGTGTGTATCTTTTTCAGCGGCGGGTTGGTTAAACCCATCAAATTAACTGTATATGATTTTCTTTTTTCAACCTGTTTAt              >  2:80895/1‑140 (MQ=255)
                                                                                                                        aCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa            <  1:3509126/140‑1 (MQ=255)
                                                                                                                        aCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa            <  1:3575791/140‑1 (MQ=255)
                                                                                                                        aCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATCCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa            <  1:3789440/140‑1 (MQ=255)
                                                                                                                           tGGTCGTGTTTTTTATGCAGGAAGACGGGTAATGCCAATTTTTAGTTTTAAAGAAAGTGTCTATCTTTTTGAGCGGGTTTTTAGTTTACCCCTTCAAATATACAGTATATGAATTGCTTTTTTGAACATGGTGAAAAATg         >  2:865055/1‑140 (MQ=255)
                                                                                                                            tgTCGTTTTTTTGACGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATTTTTTGGCGCTGCTGTTTTGTTATACCCTTCATATTCAAGGTATATGATGTGCGTTTTACAACCTGTTTATAACTGc        >  2:4825248/2‑140 (MQ=255)
                                                                                                                            ggTCGTTTTTTTGTTGCTGGATGACGTGTAATTCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTTCAGCTGCTGTTTAGTTAAACCCTTAATATTAACTGTATATTATGTGCTTTTTTAAACATTTTTATaaattc        >  2:990490/1‑136 (MQ=255)
                                                                                                                            ggTCGTTTTTTTGATGCTTGATTACGTGTAATTCCATTTTTTATTTTTAAATAAAGTGTCTATCTTTTTAAGCTGCATTTTCGTTAAACCCTTCATATTGACTTTATATGAATTTCTTTTTTAAACCGGTTGATTAATGc        >  2:326470/1‑140 (MQ=255)
                                                                                                                            ggTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                >  2:3918066/1‑100 (MQ=255)
                                                                                                                             gTCGTTTTTTTTAAGCTGGATGACGTGTAATGCCAGTTTTTATATTTAAAGAAAGTGTGTATCTGTTGCAGCTGCTGTTTCGTTAACACCATCATAATAACTCTATATTATTTGCTTTTTTCAACCTGTTGATaaattcc       >  2:3589935/1‑135 (MQ=255)
                                                                                                                             gTCGTGTTTTTGATTCTGGATGACGTGTAAATCCAATTTTTATTTTTAAATAAAGTGGCGATCGTTTGCAGCTGCTGTTTCGTTAAACCCTTAATATTAACAGTATATGATGTGCTTTTGTAAAACTGTTGATAAATGcc       >  2:2741975/1‑140 (MQ=255)
                                                                                                                                 tttttttGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGAt   <  2:4118226/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                   
CTGCTGTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATA  >  NZ_CP034943/3496248‑3496517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: