Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,498,677 G→T 5.1% intergenic (‑172/‑95) manA ← / → EO946_RS18005 mannose‑6‑phosphate isomerase, class I/spore germination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,498,6770GT 5.1% 820.6 / 5.8 298intergenic (‑172/‑95)manA/EO946_RS18005mannose‑6‑phosphate isomerase, class I/spore germination protein
Reads supporting (aligned to +/- strand):  ref base G (155/117);  new base T (2/23);  total (158/140)
Fisher's exact test for biased strand distribution p-value = 1.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

TTATTTTCCCGGTGCGCTCCCCTTACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTG  >  NZ_CP034943/3498539‑3498813
                                                                                                                                          |                                                                                                                                        
      tCCCGGTGCGCTCCCCTTACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGgtttgttt                                                                                                                                   <  1:2100962/140‑1 (MQ=255)
           gTGCGCTCCCCTTACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTGTGTTTCAGcg                                                                                                                              >  1:4045602/1‑139 (MQ=255)
            tGCGCTCCCCTTACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCt                                                                                                                             <  1:1523817/140‑1 (MQ=255)
              cgcTCCCCTTACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCttt                                                                                                                           <  2:3091577/140‑1 (MQ=255)
                 tCCCCTTACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTcc                                                                                                                        <  1:4791963/140‑1 (MQ=255)
                 tCCCCTTACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTcc                                                                                                                        <  1:1737099/140‑1 (MQ=255)
                      ttACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATg                                                                                                                   <  1:4565237/140‑1 (MQ=255)
                      ttACAAACGCCGGATAAGCTGTCTTTGTCCTATGACAACATATGTACAAAGCATTATGAATCAATGACCCCTTTTGAGAACACGCTTCCTTTTTATTATAGCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATg                                                                                                                   <  1:3565909/140‑1 (MQ=255)
                      gtACAAACGCCGGATAAGCTGTCTTTGTCCTATGACAACATATGTACAAAGCATTAGGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATg                                                                                                                   <  1:3877977/139‑1 (MQ=255)
                        aCAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATgaa                                                                                                                 <  2:1966396/140‑1 (MQ=255)
                         cAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATgaag                                                                                                                <  1:1178574/140‑1 (MQ=255)
                         cAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATgaag                                                                                                                <  1:538476/140‑1 (MQ=255)
                         cAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATgaag                                                                                                                <  1:3067328/140‑1 (MQ=255)
                             cGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGaata                                                                                                            >  2:4308320/1‑140 (MQ=255)
                                    tAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTcc                                                                                                     <  2:1089438/140‑1 (MQ=255)
                                          gTCTTTTTCCTATGACAACATATGTACCAAGCATTATGTATCCTTGACCCCTTTTGAGAAAACGCTTCCTTTTTATTAGATCACTTTCAATAGTAGGTTTGTTTGAGCCTTGTCCTGATGAAGAATAATTCTCCtttgtt                                                                                               >  1:2372896/1‑140 (MQ=255)
                                          gTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCtttttt                                                                                               >  1:5567633/1‑140 (MQ=255)
                                          gTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCGTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCtttttt                                                                                               >  1:5308200/1‑140 (MQ=255)
                                          gTCTTTGTCCTATGACAACATAGGTACAAAGCATTATGTATCAATGACACCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTGTCAATAGAAGGTTTGTTTCATCCTTTTCCTTATGACGAATAATTCTCCtgtttt                                                                                               >  1:5535368/1‑140 (MQ=255)
                                            cTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGa                                                                                             >  1:2755583/1‑140 (MQ=255)
                                              ttttCCTATGACAACATATGTACAAATCATTATGTATAAATGACACATTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATa                                                                                           <  2:2972807/140‑1 (MQ=255)
                                                     aTGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAAtt                                                                                     <  2:285106/139‑1 (MQ=255)
                                                     aTGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAAtt                                                                                     >  1:285106/1‑139 (MQ=255)
                                                     aTGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTa                                                                                    >  2:2913611/1‑140 (MQ=255)
                                                             atatGTACAAAGCATTATGTATCAATGAACCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAAc                                                                            <  2:1449991/140‑1 (MQ=255)
                                                                  tACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCa                                                                       >  1:5124722/1‑140 (MQ=255)
                                                                  tACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTGTTTAATATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAGTAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCa                                                                       >  1:5083644/1‑140 (MQ=255)
                                                                    cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                     >  1:1953496/1‑140 (MQ=255)
                                                                    cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                     <  2:4123056/140‑1 (MQ=255)
                                                                    cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                     <  2:4879334/140‑1 (MQ=255)
                                                                    cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGCTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                     >  2:952137/1‑140 (MQ=255)
                                                                    cAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTATTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                     <  2:1728110/140‑1 (MQ=255)
                                                                    cAAAGCATTATGTATCAATCACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAAt                                                                     <  2:5541229/140‑1 (MQ=255)
                                                                      aaGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCt                                                                   >  1:2629326/1‑140 (MQ=255)
                                                                       aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGACGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                  >  2:3008561/1‑140 (MQ=255)
                                                                       aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGTTACAAATTAAGAAAAACCGTCAATCTg                                                                  >  1:1862540/1‑140 (MQ=255)
                                                                       aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAATACCGTCAATCTg                                                                  >  1:2490973/1‑140 (MQ=255)
                                                                       aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                  >  1:2203648/1‑140 (MQ=255)
                                                                       aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                  >  2:2490686/1‑140 (MQ=255)
                                                                       aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                  >  1:673985/1‑140 (MQ=255)
                                                                       aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                  >  1:695417/1‑140 (MQ=255)
                                                                       aGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTg                                                                  >  1:929228/1‑140 (MQ=255)
                                                                            tATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAATAAAAAACGTAAATCTGTTTta                                                             >  2:3971906/1‑140 (MQ=255)
                                                                            tATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTta                                                             >  2:5310318/1‑140 (MQ=255)
                                                                            tATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTta                                                             >  2:509191/1‑140 (MQ=255)
                                                                            tATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTta                                                             >  2:2125797/1‑140 (MQ=255)
                                                                            tATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTta                                                             >  2:1306650/1‑140 (MQ=255)
                                                                            tATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTATCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTta                                                             >  2:1537385/1‑140 (MQ=255)
                                                                            tATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCATTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTta                                                             >  2:4589980/1‑140 (MQ=255)
                                                                             aTGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTtat                                                            <  2:1113/140‑1 (MQ=255)
                                                                                 aTCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAg                                                        <  1:3019173/140‑1 (MQ=255)
                                                                                 aTCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAg                                                        >  2:3397884/1‑140 (MQ=255)
                                                                                 aTCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAg                                                        <  1:3110385/140‑1 (MQ=255)
                                                                                 aTCAATGACCACTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAg                                                        <  1:1062188/140‑1 (MQ=255)
                                                                                   cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGTAGGTTTGTTTCAGCCTTTGCCTTATGAAGAATAATTCTCCTTTTTTGATACAATTTTAGAAAAACCGTCAATCTGTTGTATAAAggg                                                      >  2:3720906/1‑140 (MQ=255)
                                                                                   cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                      >  2:3681131/1‑140 (MQ=255)
                                                                                   cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                      >  2:5712176/1‑140 (MQ=255)
                                                                                   cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                      >  1:4388275/1‑140 (MQ=255)
                                                                                   cAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                      >  1:1319755/1‑140 (MQ=255)
                                                                                   cAATGACCCCTTTTGAGAAAACGCTTCCTTGTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATTAACAATAATTCTCCTTTATTGATACCAATTAAGAAAAACCGTCAATCTGTTTTATAAAggg                                                      >  2:1410198/1‑140 (MQ=255)
                                                                                     aTGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAAACGTCAATCTGTTTTATAAAGGGga                                                    >  2:329848/1‑140 (MQ=255)
                                                                                      tGACCCCTTTTGAGAAAACGCGTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGgag                                                   <  2:35329/140‑1 (MQ=255)
                                                                                        aCCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGgagag                                                 >  2:22589/1‑140 (MQ=255)
                                                                                        aCCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGAAAAAATGTTTTATAAAGGGgagat                                                 >  2:1659179/1‑139 (MQ=255)
                                                                                        aCCCCTTTTGAGAAAACGCTACCTTTTTATTATATCACTTTCAATAGAATGTTTGTTTCAGCCTTTTCCTTATGAATAATAATTATACTATTTTGATAAAAATTAAGAAAAACCCTCAATATGTATTATAAAGGGcagag                                                 >  2:3246602/1‑140 (MQ=255)
                                                                                          cccTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGaa                                               >  2:4686736/1‑140 (MQ=255)
                                                                                          cccTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGaa                                               >  2:924286/1‑140 (MQ=255)
                                                                                          cccTTTTGAGAAAACGCTTCCTTTGTATTATAGCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTGATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTGTATAAATGGCAGAGaa                                               >  1:5670794/1‑140 (MQ=255)
                                                                                                ttagaAAACGCTTACATTTTATTATATCACTTTCAATAGAAGGTTTGTTTAAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATg                                         <  2:2027616/138‑1 (MQ=255)
                                                                                                      aaCGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATc                                   >  1:3200461/1‑140 (MQ=255)
                                                                                                      aaCGCTTCCTTTTTATTATATCACTTTAAATAGAAGGTTTGTTTCAGCATTTTCCTTATGAATAATAATTCTCATTTTTTGATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATc                                   <  2:2731430/140‑1 (MQ=255)
                                                                                                             ccTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTg                            <  2:3200461/140‑1 (MQ=255)
                                                                                                                 tttATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGAtct                        >  1:839841/1‑140 (MQ=255)
                                                                                                                  ttattaTATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGAtctc                       <  1:1532664/140‑1 (MQ=255)
                                                                                                                  ttattaTATCACTTTCAATACAAGGTTTGTTTCAGCATTTTCCTTATGAATAATAATTCTCCTTTTTTGATAAAAATTAACAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGAtctc                       <  1:409876/140‑1 (MQ=255)
                                                                                                                  ttattaTATCACTTACAATAGAAGGTTTGTTTCAGCCTTTTCCTTATTAACAATAATTCTCCTTTTTTGATAAAAATTAACAAAAACCGTCAATCTTTTTTATAATGGGGAGAGAAACTATGCAAATCGACTCTGAtctc                       <  1:647118/140‑1 (MQ=255)
                                                                                                                  ttattaTATCAATTTCAATCGAAGGTTTGTTTCAGCTTTTTCCTTATCAAGAATAATTCACCTTTTTTTATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGTGAGAAACTATGAAAATCGACTCTGAtctc                       <  1:633472/140‑1 (MQ=255)
                                                                                                                  ttattaTAGACCTTTCAAGAGAAGGTTTTTTTCAGCCTTGTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAACGGGGAGAGAAACTATGAAAATCGACTCTGAtctc                       <  2:5355997/140‑1 (MQ=255)
                                                                                                                     ttatATCACTTTCAATAGATGTTTTGTTTCAGCCTTTTCGTTATGAATAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTAAATCTGTTTTATAAAGGGGAGAGAAACTAGGCAAATCGACTCTGATCTCCAg                    <  2:2438057/140‑1 (MQ=255)
                                                                                                                     ttatATCAATTTCATTAGAAGGTTTGTTTCAGACTTTTCATTATGAAGAATAATTCTCGTTTTTTGATACAAATTATGAAAAACCGTCAATCTGTTTTATAAAGGGGAGTGAAACTATGCAAATCGACTCTGATCTCCAg                    <  2:4342432/140‑1 (MQ=255)
                                                                                                                       ttataACTTTAAATATAATGTTTTTTTCAGCCTTTTCCTTATTAATATTATTGGTCCTTTTTTTATACAATTTAATAAAAGGCTTCAATCTGTTTTATAAAGGGGAGAGAAAATATGCAAATCGACTCTGATCTCCAGca                  <  2:4977197/139‑1 (MQ=255)
                                                                                                                       ttatCACTTTCAATAGCAGGTTTTTTGCCGCCTTTTCCTTAGGAACAATAAGCCGCCTTTTTACAAACAAATAAAGAAAACCCGTAAATCGGTTTTATAACGGGGATAGAACCTATGCAAATCGACTCTGATCTCCAGca                  <  1:3798475/139‑1 (MQ=255)
                                                                                                                       ttatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATTAAGAATAATTCTCCTTTTTTTATACAAATTAAGAAAAACCGTCAAACTGTTTTATAAAGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGca                  <  2:846843/139‑1 (MQ=255)
                                                                                                                       atatCCCTTTCAATCGAAGGTTTGTTTCAGCCCTTTCCTAATGAAGAATCATACTCCTCTTGGCATAACAATTAACAAAAACCGTAAAGCTGTTTTAAAACGGGTAGAGAAAAGATGCAAATCGACTCTGATCTCCAGca                  <  1:2913611/140‑1 (MQ=255)
                                                                                                                       atatCATTTTAAATAGAAGGTTTGTTTCAGCCTTTTCCTTATCAAGAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                  <  2:4388275/140‑1 (MQ=255)
                                                                                                                       atatCACTTTCAATAGAATGTTTTTTTCAGCGTTTTCCTTATGAACAATAATTATCATTTTTTGATACAAATTAAGAAAAACCGTCAATCTTTTTTATAAAGGGTAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                  <  2:1005416/140‑1 (MQ=255)
                                                                                                                       atatCACTTTCAATAGAAGTTTTGTTACAGCCTTTTCCTTATTAAGAATAATACTCCTTTTTGGATACAAATTAACAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                  <  2:2372896/140‑1 (MQ=255)
                                                                                                                       atatCACTTGAATTATAAGGTTTGTTTCAGCCTTTTCATTATGAACAATAATTCTCATTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                  <  2:5060137/140‑1 (MQ=255)
                                                                                                                       atatCAATTTCAATAGAAGTTTTTTTTATGCCTTTTCCTTATTAATAATAATTATCCTTTTTTGATACAAATTAATAAAAACCTTAAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                  <  1:2867560/140‑1 (MQ=255)
                                                                                                                       atatCAATTTCAATAGAAGTTTTGTTTCAGCCTTTTCCTTATTAAGAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGATAAACTATGAAAATCGACTCTGATCTCCAGca                  <  1:1611121/140‑1 (MQ=255)
                                                                                                                       atatCAATTTAAATAGAAGTTTTTTTTCAGACTTTTCATTATGAATAATAATTCTCCTTTTTTGATAAAAATTAAGAAAAACCGTCAATCTTTTTTATAATGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGca                  <  2:5765681/140‑1 (MQ=255)
                                                                                                                       atatAATTTTAAATAGAAGGTTTGTTTCAGCATTTTCCTTATTAATAATATTTCTCTTTTTTTTATAAAATTTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGATAAACTATGCAAATCGACTCTGATCTCCAGca                  <  2:452507/140‑1 (MQ=255)
                                                                                                                       atacCACTTTCAAGAGAAGGTTTTTTTCAGCCTTTACCTTATTAAGAATAATTCTCATTTTTTGATAAAAATTAAGAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAACCTATGCAAATCGACTCTGATCTCCAGca                  <  2:1867194/140‑1 (MQ=255)
                                                                                                                        tatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATATTTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                 <  2:2475411/140‑1 (MQ=255)
                                                                                                                        tatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTCATACAAATTAAGAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                 <  1:5712603/140‑1 (MQ=255)
                                                                                                                        tatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCATTTTTTGATACAATTTAAGAAAAACCGTCAATCTTTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                 <  1:4308320/140‑1 (MQ=255)
                                                                                                                        tatCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTACTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGcac                 >  1:3281994/1‑140 (MQ=255)
                                                                                                                               ttCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGAAAATCGACTCTGATCTCCAGCACAATATAg          >  2:546691/1‑140 (MQ=255)
                                                                                                                                       aaGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTg  >  1:3755873/1‑140 (MQ=255)
                                                                                                                                       aaGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTg  >  2:4725367/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TTATTTTCCCGGTGCGCTCCCCTTACAAACGCCGGATAAGCTGTCTTTTTCCTATGACAACATATGTACAAAGCATTATGTATCAATGACCCCTTTTGAGAAAACGCTTCCTTTTTATTATATCACTTTCAATAGAAGGTTTGTTTCAGCCTTTTCCTTATGAAGAATAATTCTCCTTTTTTGATACAAATTAAGAAAAACCGTCAATCTGTTTTATAAAGGGGAGAGAAACTATGCAAATCGACTCTGATCTCCAGCACAATATAGAAACATTG  >  NZ_CP034943/3498539‑3498813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: