Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,562,015 A→C 12.7% intergenic (‑661/‑137) EO946_RS18300 ← / → EO946_RS21105 response regulator/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,562,0150AC12.7% 610.9 / 3.9 282intergenic (‑661/‑137)EO946_RS18300/EO946_RS21105response regulator/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (141/86);  new base C (3/48);  total (145/137)
Fisher's exact test for biased strand distribution p-value = 1.67e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

GGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATC  >  NZ_CP034943/3561876‑3562151
                                                                                                                                           |                                                                                                                                        
gggATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACaa                                                                                                                                          <  1:4628748/140‑1 (MQ=255)
       gCAAGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGAc                                                                                                                                   <  1:1892425/140‑1 (MQ=255)
        cACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATATCGCAAAACAGACCATATAAGAGCTTTTTTAAGCTTCACAAGGGTGACa                                                                                                                                  >  1:3680771/1‑140 (MQ=255)
         aCGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACaa                                                                                                                                 <  1:5477114/140‑1 (MQ=255)
           gtcgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATg                                                                                                                               <  2:1648930/140‑1 (MQ=255)
             cgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAATGGTGACAATGaa                                                                                                                             >  2:1472349/1‑140 (MQ=255)
                      aCAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGAACATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTa                                                                                                                    <  2:1335215/140‑1 (MQ=255)
                       cAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTAc                                                                                                                   <  2:3524428/140‑1 (MQ=255)
                       cAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTAc                                                                                                                   <  2:5599086/140‑1 (MQ=255)
                        aGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACa                                                                                                                  >  2:3239527/1‑140 (MQ=255)
                            cAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTAGTGGTACAAttt                                                                                                              >  1:1242331/1‑140 (MQ=255)
                                 cGCATCTATTTTAATGGCCCATGAATAGGACACAGCGGAAGCCCTTCAAATAACCCTGCTTTTATAGCGCAAAACAGACCATATACGCGCTGTTTTACGCTTCACAAGGGTGCCAATGCACTTGTGGTACAATTTTCaaa                                                                                                         >  2:4396678/1‑140 (MQ=255)
                                 cGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCaaa                                                                                                         >  2:5033114/1‑140 (MQ=255)
                                    aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                      >  2:1607731/1‑140 (MQ=255)
                                    aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                      >  1:262769/1‑140 (MQ=255)
                                    aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTGTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                      >  2:4712268/1‑140 (MQ=255)
                                      cTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCg                                                                                                    >  1:3797564/1‑140 (MQ=255)
                                         ttttAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGaa                                                                                                 >  2:819199/1‑140 (MQ=255)
                                         ttttAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGaa                                                                                                 >  2:1056691/1‑140 (MQ=255)
                                              aTGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACg                                                                                            <  2:178735/140‑1 (MQ=255)
                                               tGGCCAATTAATAGGACAACGCGGAACCCCTTCAAATAACCCTGTTTTTATCGCGCAAACCACACCATATACGCGGTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGc                                                                                           <  2:2229458/140‑1 (MQ=255)
                                               tGGACAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACAATATCCGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGc                                                                                           <  2:762885/140‑1 (MQ=255)
                                                     aTTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTAGGAAAATCGACGGGGt                                                                                     >  1:4084179/1‑140 (MQ=255)
                                                         aTAGCACAAAGCGCAACCCCTTCAAAAAAACCTGATTTTATAGCCCAAACCACACCATAAACGAGCTTTTTTACGCTACACACGGGGCACAAGCAACTGGGGGTACAATTTTCAAACTACGGAAAAACGCCGGGGTgggg                                                                                 <  1:4418325/140‑1 (MQ=255)
                                                           aGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTGTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGaa                                                                               >  1:3888267/1‑140 (MQ=255)
                                                            ggACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAAt                                                                              >  1:1491112/1‑140 (MQ=255)
                                                                aaaGCGGAAGCTCTTAAAATAAACTTTATTTTTTACGGCAAACCAGACCATTTACGCCGTTTTTTACGCTTCACAAGGGTTACAATGAAATTGTGGTACAATTTTCAAAGTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                          <  2:4663323/140‑1 (MQ=255)
                                                                aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGTAATCGCa                                                                          >  1:1588438/1‑140 (MQ=255)
                                                                aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                          >  1:4765669/1‑140 (MQ=255)
                                                                aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                          >  2:4525947/1‑140 (MQ=255)
                                                                aaaGCGCAAGCCCTTAAAATAAACCTGTTTTTATCGCCAAAACCCCACCATAAACGAGCTTTTTTACGCTTCACACGGGTCAAAATGAACTTGTGGTAAAATTTTCAAACTGCGCAAAAACGCCGGGGTGGGGAATCGCa                                                                          <  1:451327/140‑1 (MQ=255)
                                                                  aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGACACACGCCGGGGTGGGGAATCGCaaa                                                                        >  1:1768136/1‑140 (MQ=255)
                                                                  aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                        >  2:1526320/1‑140 (MQ=255)
                                                                       aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGTCAATGAACTTGTGGTACAATTTTCAAACTTCGGAAACACGCCGGGGTGCGGACTCGCAAAGCCGa                                                                   >  1:5549306/1‑140 (MQ=255)
                                                                       aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                   >  1:995504/1‑140 (MQ=255)
                                                                       aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                   >  1:2849549/1‑140 (MQ=255)
                                                                       aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAAATTGTGGTACAATTTTCAAAATACGGAAAAACGCCGCGGTGGGCAATCGCAAAGCCGa                                                                   >  2:3901913/1‑140 (MQ=255)
                                                                       aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTGTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCgt                                                                   >  1:5000830/1‑139 (MQ=255)
                                                                        agacacacAAATAAACCTGATTTTATACCCAAAAACACACCATATACGAGATTTTTTACGCTACACACGGGTGAAAATGAACTTGTGGTAAAATTTACAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                  <  1:5384285/133‑1 (MQ=255)
                                                                        aGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATCCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTGCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                  <  2:4621875/140‑1 (MQ=255)
                                                                        aGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATCCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTAAAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                  <  2:116493/140‑1 (MQ=255)
                                                                        aGCCATTCAAATAAACATGATTTTATAGCGCAAAACCCACCATATCCGAGCTTTTTTACGAGTCACAAGGGTGACAATGAACTTGTTGTACAATTTTCAAACTTCGGAAAAACCCCGGGTTGGGGAATCGCAAAGCCGAt                                                                  <  2:318108/140‑1 (MQ=255)
                                                                            cTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACt                                                              >  1:181034/1‑140 (MQ=255)
                                                                              tCAAATAACCCTGCTTTTCTAGCGCAAACCAGACCAGATCGGAGCTTTTTGACGCTTCCCAAGGGTTACACTGAACGTGGGGTCCAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                            <  2:5344261/140‑1 (MQ=255)
                                                                              tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                            >  1:1334170/1‑140 (MQ=255)
                                                                              tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                            >  1:3058510/1‑140 (MQ=255)
                                                                              ccAACTAAACCGCATTTTATAGCGCAAACCCGACCATATCCGAGCTTTTTTACGCTTCACACGGGTCACAAAGACCTTGTGGTACAATTTTCAACCTCCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                            <  2:4823430/139‑1 (MQ=255)
                                                                                aaaTAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGGGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTg                                                          <  2:1193519/140‑1 (MQ=255)
                                                                                   tAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                       <  2:2622314/140‑1 (MQ=255)
                                                                                   tAAACCTGATTTTATAGCGCAAAACAGACAATATACGACCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAATCCGATTACTTTTGGaa                                                       <  2:3061435/140‑1 (MQ=255)
                                                                                   tAAACCTGATTTTATAGCGCAAAACACCCCATATACGCGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                       <  2:5185480/140‑1 (MQ=255)
                                                                                   aaaaCCTTATTTTATCGCGAAAAACACACCATATACTAGCTTTTTTACGCTACACACGGGTGACAATGAAGTTGTGGTACAATTTTCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                       <  1:5060878/139‑1 (MQ=255)
                                                                                     acccTGTTTTTATCGCCCAAACCCCACCATATACTAGCTTTTTTACGCTACACACGGGTGACAATCACCTTGTGGTACATTTTAAAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3844415/138‑1 (MQ=255)
                                                                                     aactttaTTTTATAGCCCAAAACACACCATATCCGCGTTTTTTTACGCTACACACGGGTGACAATGAACTTTTTTTACAATTTTCAACGTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:4693100/134‑1 (MQ=255)
                                                                                     aaCCTTATTTTATCGCGCAAAACACACCATATCCGAGTTTTTTTACGCTACACACGGGGGAAAATCAAGTTGTGGAACATTTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:5252804/140‑1 (MQ=255)
                                                                                     aaCCTTATTTTATAGCGAAAAACACCCCATATACGAGCTTTTTTCCGCTACCCACGGGTGACAATCAAGTTGTGGTACAATTTACAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3345236/140‑1 (MQ=255)
                                                                                     aaCCTTATTTTATAGCACAAAACACACCATATCCTAGCTTTTTTCCGCTACACACGGGTGACAATGACGTGGTGGTACAATTTAAAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:4801347/140‑1 (MQ=255)
                                                                                     aaCCTGATTTTATAGCGCAACACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  2:5506293/140‑1 (MQ=255)
                                                                                     aaCCTGATTTTATAGCGCAAACCCCACCCTATCCGAGATTTTTTACGCTAAACACGGGTGAAAATGAACTTTTGGTACAATTTACAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:2173263/140‑1 (MQ=255)
                                                                                     aaCCTGATTTTATAGCGCAAAACCGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  2:3752828/140‑1 (MQ=255)
                                                                                     aaCCTGATTTTATAGCGAAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATTAATTTGTGGTACAATTTAAAAACTACGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:1056691/140‑1 (MQ=255)
                                                                                     aaCCTGATTTTATAGCGAAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  2:2580903/140‑1 (MQ=255)
                                                                                         tttttttATAGCCCAAAACACACAATATACGCGATTTTTTCCGCTTCACACGGGTCACAATGAACTTGTGGTACAATTTTCAACCTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAgg                                                 <  1:2308389/137‑1 (MQ=255)
                                                                                         ttaTTTTATAGCGCAAAACAGACAATATACGATCTTTTTTACTCTTAACAAGGGTGACAATGAAATTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAgg                                                 <  2:188162/138‑1 (MQ=255)
                                                                                         tGATTTTATACCGCAAAACAGACAATAACCGCGGTTTTTCACGCTTCACACGGGTGACAATGAACGTGTTGTACAATTTCCAAACGGGGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAgg                                                 <  1:851348/140‑1 (MQ=255)
                                                                                          gATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGATTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGa                                                <  2:2016053/140‑1 (MQ=255)
                                                                                           tttttATCGCCAAAACCAGACCATAAACGCGATTTTTTCCGATTCACACGGGTTAAAATGAACTTGTGTTACAATTTGCAAACTGCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                               <  1:2912873/139‑1 (MQ=255)
                                                                                           aTTTTATCGCGCAAAACACACCATATACGAGCTTTTTTACGATTCACAAGGGTGACAATGAACTTGTGGTAAAATTTTCAAACTTCGGAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                               <  1:4387432/140‑1 (MQ=255)
                                                                                           aTTTTATAGCGCAAACCACACCATCTACCCGCTTTTTTAGGGTTCACACGGGTGACAATCAACTGGGGTTACATTTTTCAAACTTCGCAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                               <  1:1694940/140‑1 (MQ=255)
                                                                                           aTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                               <  2:3638204/140‑1 (MQ=255)
                                                                                             tttATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATTAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa                                             <  2:568485/140‑1 (MQ=255)
                                                                                                ataGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACat                                          >  2:3016085/1‑140 (MQ=255)
                                                                                                              aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGACTCGCAAAGCCGCTTACTTTTGGAAGGCAGGATAAAATATTGCGAGAGAACa                            >  1:4137157/1‑140 (MQ=255)
                                                                                                              aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGACCa                            >  2:3861168/1‑140 (MQ=255)
                                                                                                              aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  1:1090745/1‑140 (MQ=255)
                                                                                                              aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  1:1938087/1‑140 (MQ=255)
                                                                                                              aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  1:4018276/1‑140 (MQ=255)
                                                                                                              aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  1:5324120/1‑140 (MQ=255)
                                                                                                              aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATCAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCTAGAGAACa                            >  1:4230868/1‑140 (MQ=255)
                                                                                                                   ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                   >  1:597963/1‑112 (MQ=255)
                                                                                                                   ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                       >  1:2498770/1‑140 (MQ=255)
                                                                                                                   ataCGAGCTTTTTTAAGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                       >  1:2055438/1‑140 (MQ=255)
                                                                                                                     aCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTGAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                          >  1:3538781/1‑87 (MQ=255)
                                                                                                                        aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCtt                  >  1:1148203/1‑140 (MQ=255)
                                                                                                                        aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCtt                  >  1:2234746/1‑140 (MQ=255)
                                                                                                                        aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCtt                  >  1:2601194/1‑140 (MQ=255)
                                                                                                                        aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCtt                  >  1:606644/1‑140 (MQ=255)
                                                                                                                          cTTTTTTACGCTTCACAAGGGTGACAATGAAGGTGTGGTACAATTTTCAAACTTCGGAAAATCGCAGGGGGGGGGAATCTCAAAGCCGATTACTGTTGGAACGCAGGATAACATCTTGCGAGAGAACAAGCTGTCCttgc                >  2:2476522/1‑138 (MQ=255)
                                                                                                                          cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTc                >  1:117741/1‑140 (MQ=255)
                                                                                                                          cTTGTTTACGCTTCACCAGGGTGACAATGAACTTGTGGTAGAATTTTCAAACTTCGGAAAAACGGCGGGGTGGGGAATCGCAAAGCCGATTACGTTTGGAACTCAGCATAACATATTGCGAGAGAACAACATTTCGTTTc                >  1:2368528/1‑140 (MQ=255)
                                                                                                                            tttttACGCTTCACAAGGGTGACATTGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt              <  2:2788794/140‑1 (MQ=255)
                                                                                                                            tttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt              >  2:486353/1‑140 (MQ=255)
                                                                                                                             ttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                   >  2:4241689/1‑102 (MQ=255)
                                                                                                                              tttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa            <  2:1984644/140‑1 (MQ=255)
                                                                                                                              tttACGATTCACAAGGTTGACAATTAAGTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa            <  2:3403115/140‑1 (MQ=255)
                                                                                                                                 aCGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGa         <  2:3806315/140‑1 (MQ=255)
                                                                                                                                     ttCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGAtt       >  2:5441135/1‑138 (MQ=255)
                                                                                                                                       cacaAGTGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGTCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   <  2:5052380/140‑1 (MQ=255)
                                                                                                                                       cacaAGGGTTACAATCAACTGGTGGTAAAATTTTCAAATTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   <  2:4084179/140‑1 (MQ=255)
                                                                                                                                       cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   >  1:4877286/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GGGATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATC  >  NZ_CP034943/3561876‑3562151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: