Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,769,576 T→G 8.7% I281S (ATT→AGT)  sacX → SacY negative regulator SacX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,769,5760TG 8.7% 1184.2 / 22.7 459I281S (ATT→AGT) sacXSacY negative regulator SacX
Reads supporting (aligned to +/- strand):  ref base T (126/274);  new base G (56/3);  total (182/277)
Fisher's exact test for biased strand distribution p-value = 8.64e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 471 total aligned reads displayed.

AAGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGCGT  >  NZ_CP034943/3769437‑3769714
                                                                                                                                           |                                                                                                                                          
aaGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAt                                                                                                                                            <  1:596472/140‑1 (MQ=255)
 aGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATTAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAtt                                                                                                                                           <  2:2095199/140‑1 (MQ=255)
   cTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                         <  2:189326/140‑1 (MQ=255)
   cTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATGACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                         <  1:3006944/140‑1 (MQ=255)
     aTTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTcg                                                                                                                                       >  2:5799049/1‑139 (MQ=255)
      ttAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATGATGACACAGTCGATTGTCta                                                                                                                                      <  1:805674/140‑1 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGCCAGGGTGCCTCGCGCTGATTGTGATGACTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGAt                                                                                                                            <  1:132585/140‑1 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGAt                                                                                                                            <  1:4379815/140‑1 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTAGATTtattat                                                                                                                            >  1:2643933/1‑140 (MQ=255)
                     ccGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAtt                                                                                                                                           >  1:4818707/1‑120 (MQ=255)
                     ccGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGc                                                                                                                       <  2:5329981/140‑1 (MQ=255)
                      cGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGcc                                                                                                                      <  2:4035390/140‑1 (MQ=255)
                        aaGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAtt                                                                                                                                           <  2:3281267/117‑1 (MQ=255)
                           tCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAt                                                                                                                 <  2:539165/140‑1 (MQ=255)
                           tCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAt                                                                                                                 <  2:3081911/140‑1 (MQ=255)
                              cTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttgt                                                                                                              <  1:95982/140‑1 (MQ=255)
                              cTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttgt                                                                                                              <  2:1043074/140‑1 (MQ=255)
                                 gATGGCGTCATCATTCCTTTTATTACGCTCATGGTGACAGGGTGCCTCGCGCTGAGTGTGATGAATCCCGCCGCATATATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGc                                                                                                           <  1:2404160/140‑1 (MQ=255)
                                  aTGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                         <  1:4704306/109‑1 (MQ=255)
                                   tGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCg                                                                                                         <  1:2286272/140‑1 (MQ=255)
                                      cgtcATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAg                                                                                                      <  2:5575153/140‑1 (MQ=255)
                                       gtcATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTGTATATTTAAGATCACGCCGGCATTGGTGCCGGAGc                                                                                                     >  1:1979831/1‑140 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCCTGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    <  2:370700/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGGTGATTGTGATTAATCCCGCCGCAGCTATCATCGGCCAAATTATGACACAGTCGCGTGTGGATATTTATGATCACGCCGGCAATGTTGCCGGAggc                                                                                                    >  1:4173873/1‑138 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTGTATATTTATTATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    >  2:2223440/1‑140 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    <  1:3115526/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    <  2:1806868/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    <  1:2451654/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    <  2:5056329/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    <  2:663058/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    <  2:4491039/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    <  2:5016246/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                    >  1:1649338/1‑140 (MQ=255)
                                        tcatcaTTCCGTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGAGGAATCCCGCCGCAGCTATCCTCGGCCAAATTATGACACAGACGATTGTGTATATTTATGATCACGCCCGCAGTGTTGCCGGAGcc                                                                                                    >  2:3606544/1‑140 (MQ=255)
                                               tCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcg                                                                                             <  1:4065382/140‑1 (MQ=255)
                                                 cTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                           <  1:1543523/140‑1 (MQ=255)
                                                 cTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                           <  1:5225408/140‑1 (MQ=255)
                                                  ttttATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCCGCCAAATTATGACACAGTCGATTGTCTAGATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggcg                                                                                          <  2:3408352/140‑1 (MQ=255)
                                                   tttATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggcgg                                                                                         >  1:5307983/1‑140 (MQ=255)
                                                    ttattaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCggcggc                                                                                        <  1:1030773/140‑1 (MQ=255)
                                                    ttattaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCggcggc                                                                                        <  2:4220848/140‑1 (MQ=255)
                                                    ttattaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCggcggc                                                                                        <  1:2535893/140‑1 (MQ=255)
                                                      attaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCAt                                                                                      <  1:3568925/140‑1 (MQ=255)
                                                       ttaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGCATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGACGGCATTGTTGCCGGAGCCCTGTTCGGCGGAATc                                                                                     <  1:5623366/140‑1 (MQ=255)
                                                       ttaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATc                                                                                     <  2:2566057/140‑1 (MQ=255)
                                                         aCGTTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAAGTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTa                                                                                   <  1:1881072/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAt                                                                                                                 >  2:2753019/1‑107 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAt                                                                                                                 <  1:2753019/107‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                                <  1:3778746/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                                <  1:2849545/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                                <  1:5368812/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                                <  2:4863749/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTAGGCGGCATCTACTc                                                                                >  2:74681/1‑140 (MQ=255)
                                                                 ggggACAGGGTGCCTCGCGCTGCTTGTGATGACTCCCGCCGCATCTATCATCGGCCAAATTATGACACACTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  2:3334122/137‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  2:2808600/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  2:4576940/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  1:3477643/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  2:2432313/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  1:4861232/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  1:3929085/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  2:116221/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  2:5262843/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           <  1:1170668/140‑1 (MQ=255)
                                                                     aCAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCg                                                                                                         <  2:5448907/106‑1 (MQ=255)
                                                                       aGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTc                                                                     >  2:1351494/1‑140 (MQ=255)
                                                                       aGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATCGTTc                                                                     >  2:4679059/1‑140 (MQ=255)
                                                                         ggTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACAGAGTCGATGGTCTATATTTATGATACCGCCGGCATTGTTGGCGGAGCCCTGTTCGGCGGCATCTACTCCCCCAGCGTTCTa                                                                   >  2:439439/1‑140 (MQ=255)
                                                                         ggTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTa                                                                   >  1:1011494/1‑140 (MQ=255)
                                                                         ggTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGGCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCCTCTAATCCACCATCCTTCTa                                                                   >  2:4542662/1‑140 (MQ=255)
                                                                         ggTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGGCTATATTTATGATCACGCCGGCAGTGTTGCCGGAGGCCTGTTCGGCGGCATCTACTCCACCATCGTTCTa                                                                   >  2:5160345/1‑140 (MQ=255)
                                                                           tGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                           >  2:5692107/1‑130 (MQ=255)
                                                                             ccTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCg                                                               >  1:3829182/1‑140 (MQ=255)
                                                                                cgcgCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCt                                                            <  1:4256651/140‑1 (MQ=255)
                                                                                cgcgCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCt                                                            <  1:369807/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                     <  1:742490/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                     <  2:4737473/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                     <  2:3518239/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                     <  1:5170180/140‑1 (MQ=255)
                                                                                        ttGTGATGCTTCCCGCCGCATCTATCATCTGCCTAATTATGACACACTCGATTGTCTATTTTTATTATCAAGCCGGCATTGTTGGCGTATCCCTTTTCGGCTTCATCTACTCCACCATCGTTCTATCCGGCTTGcatcat                                                    <  2:4358001/140‑1 (MQ=255)
                                                                                             atgaATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCGATATTTATGATCACGCCGGGCTTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCCCCATCGTTCTATCCGGCTTGCATCATAGcgt                                               >  1:5764869/1‑138 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                     <  1:2073248/130‑1 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                           <  2:4098235/140‑1 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                           <  1:1400824/140‑1 (MQ=255)
                                                                                                  tCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCAGCTACTCCACCATCGGTCTATCCGGCTTGCATCATACCTTTTATg                                          >  2:2718323/1‑140 (MQ=255)
                                                                                                      gccgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTGTATATTTATTATCACGCCGGCATTGTTGGCGGATCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATACGGCTTGCATCATAGCTTTTTTGCGAt                                      >  1:5700654/1‑140 (MQ=255)
                                                                                                      gccgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGGTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGAt                                      <  1:4550390/140‑1 (MQ=255)
                                                                                                      gccgcATATATCATCGTCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGGCGGAGCCCTGTTCGGCGGAATCTACTCCACAATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGAt                                      <  1:3366435/140‑1 (MQ=255)
                                                                                                       ccgcATCTATCATCGGCCAAATTATGACCCAGTCGATTGTCGATATTTATGATCACGCCGGCCGTGTTGCCGGCGCCCGGTTGGGGGGCATCTACGGCACCATCGGTCTATCCGGCTTGGATCATAGCGTTtgtggggtt                                     >  1:4999116/1‑135 (MQ=255)
                                                                                                       ccgcATCTATCATCGGCCAAATTAGGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAGGCGAtt                                     <  1:4604210/140‑1 (MQ=255)
                                                                                                                  atcGGCCACATGATGACACCCTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                          <  2:3981827/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                          <  1:5488936/140‑1 (MQ=255)
                                                                                                                     ggCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGGATCATAGCTTTTATGCGATTGAAGCAACgctgct                       >  2:3882103/1‑140 (MQ=255)
                                                                                                                          aaTTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTAGGCGGCATCAACGCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAAAGCTGCTTGCTg                  >  2:954578/1‑140 (MQ=255)
                                                                                                                          aaTTATGACACAGTCGAGTGTCTATATTTATGATCACCCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCCTCGTTCTATACGGCTTGCATCATATCTTTTATTCGATTGAAGCAACGCTGCGTGCTg                  >  1:2600969/1‑140 (MQ=255)
                                                                                                                              aTGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATcc              <  2:1011494/140‑1 (MQ=255)
                                                                                                                                     aGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATc                                                                 >  2:2004826/1‑82 (MQ=255)
                                                                                                                                     aGTCGATTGTCTATATTTATGATAACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATACGGCTTGCATCATAGCTTTTATGCGATTTAAGCAACGCTGCTTGCTGATACGCGTGTc       >  2:2615942/1‑140 (MQ=255)
                                                                                                                                       tCGATTGGCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGGTCGGCGGCATCTACTCCACCAACGTTCTATACGGCTTGCATCATTGCTTTTATTCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCgg     >  1:5524163/1‑140 (MQ=255)
                                                                                                                                          aTTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTTTCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGCGt  <  2:1706471/140‑1 (MQ=255)
                                                                                                                                          aTTGTCTATATTTATGATCACGCCGGCATTGGTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTGGCTGATCCGCGTGTCGGCGt  <  2:1497823/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AAGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGCGT  >  NZ_CP034943/3769437‑3769714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: