Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,796,125 C→A 8.1% L8F (TTG→TTT EO946_RS19550 ← ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,796,1250CA 8.1% 1218.6 / 20.5 464L8F (TTG→TTTEO946_RS19550ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base C (165/244);  new base A (3/52);  total (168/296)
Fisher's exact test for biased strand distribution p-value = 2.71e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 468 total aligned reads displayed.

CGTCAGGAAGGTTTCCTCCGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCAGTCTGTATA  >  NZ_CP034943/3795986‑3796262
                                                                                                                                           |                                                                                                                                          
cGTCAGGAAGGTTTCCTCCGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCCTTCTTTCTGc                                                                                                                                            >  2:317211/1‑140 (MQ=255)
cGTCAGGAAGGTTTCCTCCGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGc                                                                                                                                            >  2:1214804/1‑140 (MQ=255)
  tCAGGAAGGTTTCCTCCGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCaa                                                                                                                                          <  2:37463/140‑1 (MQ=255)
       aaGGTTTCCTCCGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCg                                                                                                                                     <  2:265733/140‑1 (MQ=255)
           tttCCTCCGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTat                                                                                                                                 <  1:1981395/140‑1 (MQ=255)
           tttCCTCCGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTat                                                                                                                                 <  1:2286975/140‑1 (MQ=255)
             tcctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGCTCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatca                                                                                                                               <  2:1937780/140‑1 (MQ=255)
             tcctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatca                                                                                                                               <  2:831674/140‑1 (MQ=255)
             tcctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatca                                                                                                                               <  2:5455506/140‑1 (MQ=255)
             tcctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatca                                                                                                                               <  2:3961269/140‑1 (MQ=255)
             tcctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatca                                                                                                                               <  2:3831139/140‑1 (MQ=255)
             tcctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatca                                                                                                                               <  1:5717804/140‑1 (MQ=255)
             gcctccGTGGTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAAGCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatca                                                                                                                               <  2:5211692/139‑1 (MQ=255)
               ctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatcatc                                                                                                                             <  2:4997119/140‑1 (MQ=255)
               ctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatcatc                                                                                                                             <  1:4702357/140‑1 (MQ=255)
               ctccGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTatcatc                                                                                                                             <  2:5376793/140‑1 (MQ=255)
                    tgtTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCtt                                                                                                                        <  1:988680/140‑1 (MQ=255)
                    tgtTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCtt                                                                                                                        >  2:1664827/1‑140 (MQ=255)
                     gtTCATTGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTc                                                                                                                       >  1:2708204/1‑140 (MQ=255)
                     gtTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTc                                                                                                                       >  1:4613503/1‑140 (MQ=255)
                     gtTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGATTACCGCTTTTACACCCATCGAGCCAGACTTTCTGCCATAGCGTTATCATCACCTTc                                                                                                                       >  2:1376172/1‑140 (MQ=255)
                       tCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCAt                                                                                                                     <  2:4250531/140‑1 (MQ=255)
                         aaTGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGc                                                                                                                   >  1:2892316/1‑140 (MQ=255)
                         aaTGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGc                                                                                                                   >  2:2954143/1‑140 (MQ=255)
                               ttCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGcac                                                                                                             <  1:1422835/140‑1 (MQ=255)
                               ttCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGcac                                                                                                             <  1:1847580/140‑1 (MQ=255)
                               ttCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGcac                                                                                                             <  2:3235612/140‑1 (MQ=255)
                               ttCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGcac                                                                                                             <  2:4690893/140‑1 (MQ=255)
                               ttCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGcac                                                                                                             <  1:4502111/140‑1 (MQ=255)
                               ttCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGcac                                                                                                             <  1:2852998/140‑1 (MQ=255)
                               ttCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGcac                                                                                                             <  1:1022982/140‑1 (MQ=255)
                               ttCCGGCATAAAATAAATCGTCAGAGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGcac                                                                                                             <  1:3031241/140‑1 (MQ=255)
                                tCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCCTCGCGATCGGGAGCCCGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTCTCATCACCTTCATGCGTGcacc                                                                                                            >  1:5341784/1‑140 (MQ=255)
                                tCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGGTATCATCACCTTCATGCGTGcacc                                                                                                            >  2:1476709/1‑140 (MQ=255)
                                   ggCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCaccacc                                                                                                         <  1:1087270/140‑1 (MQ=255)
                                              aatGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGAc                                                                                              <  1:2967609/140‑1 (MQ=255)
                                              aatGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGAc                                                                                              <  1:5307164/140‑1 (MQ=255)
                                               atGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACg                                                                                             >  2:3056005/1‑140 (MQ=255)
                                                    cAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCAGTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTGTAGATAGACGTCTTc                                                                                       >  2:5270947/1‑140 (MQ=255)
                                                     aGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCa                                                                                      >  2:2188926/1‑140 (MQ=255)
                                                         ggtgtgTCAATCCCACGATCATAATCGCGATCGGCAGCCAGATGAGCTTCCCGCTTTTCCACCCATCGAGCCATTCTTTCTGAAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCg                                                                                  <  2:3497973/138‑1 (MQ=255)
                                                         gCTGTGTCAATCCCACGATCATAATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCTTCGAGCCATTCTTTCTGAAATAGCGTTATCATCACCTTCATGGGTGCACCACCTTTATATAGCCGTCGTCAAGCg                                                                                  <  2:577891/140‑1 (MQ=255)
                                                            gtgtCAATCCCACGAGCATCATCGCGATCGGCAGCCAGATGATCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTGTCATCACCTTCATGCGTGCCCCACCTTTAGATAGACGTCTTCACGCGACt                                                                               >  2:2283044/1‑140 (MQ=255)
                                                             tgtCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTc                                                                              >  1:684086/1‑140 (MQ=255)
                                                             tgtCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTc                                                                              >  2:689795/1‑140 (MQ=255)
                                                             tgtCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTc                                                                              >  2:3863704/1‑140 (MQ=255)
                                                                       aCGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCCCCACCTTTAGATAGACGTCTTCAAGCGACTCCGTGTTCTGt                                                                    >  1:3728561/1‑140 (MQ=255)
                                                                       aCGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGt                                                                    >  1:1523828/1‑140 (MQ=255)
                                                                       aCGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGt                                                                    >  1:898933/1‑140 (MQ=255)
                                                                            catcatcGCGATCTGCCGCCCTATGAGGTTCCCGCTTGGCCACCCATCGAGCCTTTGTTTCTGCATTAGCGTTATCATCACCGTCATGCGTGCACCAGCTTTCGAGCGCCGTCTTCTAGCGACTCCGTTTTCTGTTCaaa                                                               <  1:3128789/140‑1 (MQ=255)
                                                                                  cgctATCGGCAGCCATATGAGCTTACCGCTTTTCCCCCCTTCGAGCATTTCTTTCTGAAATAGCTTTATCATCACCTTCATGGGTGCACCCCCTTTAGATCGACGTATTCAAGCGACTCCGTTTTCTGTTCAAAATGGGt                                                         <  1:1234092/140‑1 (MQ=255)
                                                                                   gctaTCGGCAGCCAGATGCGATTACCGCTTTACCACCCAGCGAGCCATTCTTTCTGAAATCGCGTTATCATCACCTTCATGCGTGCACCCCCTTTAGATACACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAGCGGGTg                                                        <  1:1592352/137‑1 (MQ=255)
                                                                                   gctaTCGGCAGCCAGATGAGATTACCTCTTTTCCACCCATGGAGCCATTCTTTCTGAAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTCGATAGACGTATTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTg                                                        <  1:5572879/137‑1 (MQ=255)
                                                                                    cgATCGGCAGCAAGTTGAGCTTACCGCTTTTCCACCCATCGAGCCATTGTTTCTGCAATAGCGTTATCACCACCTTCAGGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGa                                                       <  1:1181851/140‑1 (MQ=255)
                                                                                      ctCGGCAGCCAGATGTGCTTACCGCTTTGCCCCCCAGCGAGCCATTCTTTCTGAAATAGCGTTATAATCACCTCCATGCGGGCACCCCCTTTAGATAGACGTCTACAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACa                                                     <  2:2584180/139‑1 (MQ=255)
                                                                                            aGCCAGATGAGCTTACCGCTTTTCCCCCCATCGAGCCATTCTTTCGGCAATAGCGTTATAATCACCTACATGCGGGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAAt                                               <  2:5142480/140‑1 (MQ=255)
                                                                                              ccAGATGAGCTTACCGCTTTTCCACCCCTCGAGCCTTTATTTCTGCAATAGCGTTATCTACCCCTTACTGCGTGCACCACCTTCACATAGACCTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATcc                                             <  1:5225802/140‑1 (MQ=255)
                                                                                              ccAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATcc                                             >  2:3431939/1‑140 (MQ=255)
                                                                                                    gAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTg                                       <  2:1574599/140‑1 (MQ=255)
                                                                                                      gCTCACCGCTTTCCCACCCATCGAGCAATTTTTTCTGCAATCCCGTTACCATCACCTTCATGCGTGCACCACGTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGa                                     <  1:4861760/140‑1 (MQ=255)
                                                                                                      ccTTACCGCTTTTCCCCCCCCCGAGCCATTCTTTCTGAAATCCCGTTATCACCCCCTGCCTGCGTGCACCACCTTTAGATAGACGTCTTCACGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGa                                     <  1:379693/139‑1 (MQ=255)
                                                                                                       cTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCACATTTGGAt                                    >  2:2739647/1‑140 (MQ=255)
                                                                                                        ttACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATa                                   >  1:5656995/1‑140 (MQ=255)
                                                                                                         tACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATAc                                  >  2:5525426/1‑140 (MQ=255)
                                                                                                           ccGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGGGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACaa                                <  2:3092652/140‑1 (MQ=255)
                                                                                                            cGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAAt                               >  2:4938554/1‑140 (MQ=255)
                                                                                                            cGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCACATTTGGATACAAt                               >  2:3368607/1‑140 (MQ=255)
                                                                                                                tttCCACCCATCGCGCCTTTCTTTCTGAAATAGCGTTATCATCACCTCCATGCGGGCACCCCCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACt                           <  1:2552803/140‑1 (MQ=255)
                                                                                                                tttCCACCCATCGAGCCATTCTTTCTGCAATAGCTTTATAATCACCTTCATGCGTGCACCACATTTAGATACCCGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACt                           <  2:2506770/140‑1 (MQ=255)
                                                                                                                tttCCACCCATCGAGCAATTCTTTCTGAAATAGCGTTATAATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTAAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACt                           <  1:3961407/140‑1 (MQ=255)
                                                                                                                tttCCACCCAGCGAGCCATTCTTTCTGCAATCGCGTTATCATCCCCTTCATGCGAGCACCAGCTTTAGATCGAGGTCTTCAAGCGACTCCGTTGTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACt                           <  1:317211/140‑1 (MQ=255)
                                                                                                                 ttCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGTGTGACAGTCAATCCCATTTGGATAAaatcaatc                          >  1:3597036/1‑140 (MQ=255)
                                                                                                                 ttCCACCCATCGAGCCATTCTTTCTGCAATAGCGGTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCACGCGACTCCGTTTTCTGTTCAAACTGGGTGACAGTCAATCCCATGTGGATACAATCACTc                          >  2:4511912/1‑140 (MQ=255)
                                                                                                                    cacccaccGAGCATTTCTTTCTGAATTAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTTTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGc                       <  1:4226847/140‑1 (MQ=255)
                                                                                                                    acccaaTCTAGCCATTGTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACATTTAGATAGCGTACTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGc                       <  1:1705168/135‑1 (MQ=255)
                                                                                                                         aTCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGt                  >  1:4100997/1‑140 (MQ=255)
                                                                                                                         aTCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGt                  >  1:2574762/1‑140 (MQ=255)
                                                                                                                           cGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCCTTTGGATACAATCACTCAGCAATGTcc                >  1:5463788/1‑140 (MQ=255)
                                                                                                                           cGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTcc                >  1:2409843/1‑140 (MQ=255)
                                                                                                                              gcaaTTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGAACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGc             <  2:4613503/137‑1 (MQ=255)
                                                                                                                              gcaaTTCTTTATGCAATTGCGTTATCATACCCTTCTTGCGTGCACCACGTTTATATAGACGTGTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGc             <  1:4004453/137‑1 (MQ=255)
                                                                                                                              gCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTAAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGc             <  2:2708204/140‑1 (MQ=255)
                                                                                                                               ccATTCTTTCTGCAATCGCGTTATCAACACCTCCCTGCGTGCACCACCTTTAGATACACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGcc            <  2:563595/140‑1 (MQ=255)
                                                                                                                               caaTTCTTTCTGCACTCGCTTTATCATCACCTGCATGCGTGACCCACATTTAGATAGACGTCTTCACGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGcc            <  2:4039796/138‑1 (MQ=255)
                                                                                                                               caaTTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTAACGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGcc            <  2:1314814/138‑1 (MQ=255)
                                                                                                                                cATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGAGAGACGTCTTCACGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCa           <  1:2057621/140‑1 (MQ=255)
                                                                                                                                cATTCTTTCTGCAATAGCGTAATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGACTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCa           <  2:1778625/140‑1 (MQ=255)
                                                                                                                                cATTCTTTCTGCAAGAGCTTGATCATCACCTTCATGCGGGCACCCCCTTTCGATAGACGTCTACAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCa           <  2:2849668/140‑1 (MQ=255)
                                                                                                                                cATTCTTTCTGAAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCa           <  2:4868673/140‑1 (MQ=255)
                                                                                                                                cATTCTTTCTGAAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCa           <  2:1849756/140‑1 (MQ=255)
                                                                                                                                cATTCTTTAGGAAATAGCGTTATCATCACCTTCATGCGTGAACCACCTTTACATCGCCGTCTACAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCa           <  1:2189059/140‑1 (MQ=255)
                                                                                                                                  ttctttctGCAATAGCGTTATCATCACCTTCAGGCGTGCACCACCTTTAGATAGACGGCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCAGt         <  1:4773673/140‑1 (MQ=255)
                                                                                                                                  ttctttctGCAATAGCGTTAACATCACCTTCATGCGGGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCAGt         <  2:5731265/140‑1 (MQ=255)
                                                                                                                                   tctttctGCAATCGCTTTATCATCACCTTACGGCGTGCACCACCTTTAGATCGACTTCGTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCAGTc        <  2:3675679/140‑1 (MQ=255)
                                                                                                                                   tctttctGAAATAGCGTTACCATCACCTTCCTGGGTGCACCACCTTTAGATAGCCGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCAGTc        <  1:804335/140‑1 (MQ=255)
                                                                                                                                   tctttatGAAATAGAGTTATCATCAATTTGTTTCGTGCACAACCTTTATATTGACTAATACTAGCGACTGCGTGTTATGTTCAAAATGGGTGACAGTCTATCACATTTGGATACAATCACTCCTCAATGTCCGGCCAGTc        <  2:4140931/140‑1 (MQ=255)
                                                                                                                                    ctttctGAACTAGCGTTATCAGAACCTTACTGCGTGCACCACCTTTAGATAGTGGAATTCACGCGACTACGTTTTCTGTTCAAAATGGGTGTCAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCCGTCt       <  1:5007824/140‑1 (MQ=255)
                                                                                                                                       tctGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCa           >  1:1110665/1‑133 (MQ=255)
                                                                                                                                         tGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCAGTCTGtata  >  1:4987657/1‑140 (MQ=255)
                                                                                                                                         tGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCAGTCTGtata  >  1:3415561/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CGTCAGGAAGGTTTCCTCCGTGTTCAATGATTTCCGGCATAAAATAAATGGTCAGCGGCTGTGTCAATCCCACGATCATCATCGCGATCGGCAGCCAGATGAGCTTACCGCTTTTCCACCCATCGAGCCATTCTTTCTGCAATAGCGTTATCATCACCTTCATGCGTGCACCACCTTTAGATAGACGTCTTCAAGCGACTCCGTTTTCTGTTCAAAATGGGTGACAGTCAATCCCATTTGGATACAATCACTCAGCAATGTCCGGCCAGTCTGTATA  >  NZ_CP034943/3795986‑3796262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: