BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,220	G→T	7.4%	T52K (ACG→AAG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,220	0	G	T	7.4%	1084.4 / 18.4	388	T52K (ACG→AAG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (244/99); new base T (2/42); total (246/142)
Fisher's exact test for biased strand distribution p-value = 2.07e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

GAAAGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGC  >  NZ_CP034943/3851083‑3851355

  aaGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttat                                                                                                                                     <  1:3948381/140‑1 (MQ=255)

  aaGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttat                                                                                                                                     <  1:5149770/140‑1 (MQ=255)

  aaGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttat                                                                                                                                     <  1:5481847/140‑1 (MQ=255)

     agaAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAc                                                                                                                                  <  1:5190959/140‑1 (MQ=255)

     agaAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAc                                                                                                                                  <  1:1436516/140‑1 (MQ=255)

     agaAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAc                                                                                                                                  <  2:1087794/140‑1 (MQ=255)

       caGTCCTGCAATGTCCCCAGCTGCTCGCGGTAATATGGATTGGACATGGAATTCTCTTGTCGCTAGTTTTATCACCTTGTAAGCATCACCTTCAAAGTACTGGGACAGGTCTTCTAGAAATGAACCAGTCCTTATTAGCt                                                                                                                                <  1:4025488/139‑1 (MQ=255)

       aaGTCCTGCAATGTCCCACTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                <  2:1610804/140‑1 (MQ=255)

       aaGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                <  1:4409394/140‑1 (MQ=255)

       aaGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                <  1:3300097/140‑1 (MQ=255)

       aaGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                <  1:2383966/140‑1 (MQ=255)

       aaGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                <  2:2023790/140‑1 (MQ=255)

       aaGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                <  2:730983/140‑1 (MQ=255)

         gTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGt                                                                                                                              >  2:3722714/1‑140 (MQ=255)

         gTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCGGt                                                                                                                              >  1:5282356/1‑140 (MQ=255)

            cTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATc                                                                                                                           >  1:3678873/1‑140 (MQ=255)

               cAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTGTTCTATAAATGAACCAGTCGTTATTACCTGTATCATg                                                                                                                        >  1:2463397/1‑140 (MQ=255)

               cAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGtt                                                                                                                                       <  2:4106021/125‑1 (MQ=255)

                   gTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTTTTACCTGTATCATGAGTc                                                                                                                    >  1:5334837/1‑140 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                   >  2:4165863/1‑140 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                   >  1:4550721/1‑140 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                   >  2:1801568/1‑140 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                   >  2:2118053/1‑140 (MQ=255)

                      ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                 >  2:2657948/1‑140 (MQ=255)

                      ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGAt                                                                                                                 >  2:5642884/1‑140 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGCTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                <  1:4324026/140‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                <  1:4161921/140‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                <  2:1290913/140‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                <  1:2419213/140‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                <  1:319128/140‑1 (MQ=255)

                        aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                               <  2:1573622/140‑1 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                         >  2:5199181/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                         >  2:4046470/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                         >  2:697848/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                         >  2:2404794/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                         >  1:2589209/1‑140 (MQ=255)

                                   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                    >  1:2052943/1‑140 (MQ=255)

                                   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAAACAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                    >  2:2421623/1‑140 (MQ=255)

                                           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                            <  1:349342/140‑1 (MQ=255)

                                             atcggAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTAAAATGATTCGAGCGTATTCAAGACCGTCCCaaaaa                                                                                          >  1:3634291/4‑140 (MQ=255)

                                             aTTGGAACTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTAATCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCGGGATCAGGATGCGATCGTATGCACGCCCTTCCCcaaaa                                                                                          >  1:1595776/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCaaaaa                                                                                          >  2:5322550/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAc                                                                                                                                  <  1:2138356/100‑1 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                          >  1:2774789/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                          >  1:4011012/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                          >  1:4659905/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                          >  1:5672605/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                          >  1:968164/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                          >  2:3012422/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                          >  1:1913848/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAACCCCTTCCCaaaaa                                                                                          >  2:4576489/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCCaaaaa                                                                                          >  2:4229937/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGGATTACCTGTATCATTATTCGATCGTAGGCAAGCCCGTCCCaaaaa                                                                                          >  1:76192/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCGTCTATAAATGAACCAGTCGTGATTAACAGTATCATGATTCGATCGTATGCAAGCCCTACCCaaaaa                                                                                          >  1:5719296/1‑140 (MQ=255)

                                              ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTGCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAAc                                                                                         >  1:1005281/1‑140 (MQ=255)

                                               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCACGCCCTTCCCAACAACg                                                                                        >  1:2317659/1‑140 (MQ=255)

                                               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCAAAAACg                                                                                        >  1:2087002/1‑140 (MQ=255)

                                                ggAAATGGAATTCTCTTTTCGGTAGTTTTATCACGTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTATTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTTCCCAAAAACGa                                                                                       <  2:2535582/140‑1 (MQ=255)

                                                       gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                <  2:5695607/92‑1 (MQ=255)

                                                           tctcTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGActc                                                                            >  1:4095936/1‑140 (MQ=255)

                                                                 ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAACGATAAAACGACTCTGcttc                                                                      >  2:900865/1‑140 (MQ=255)

                                                                           ttATAACTTTTTAAGCATAACGTCAAAATTAGTCGGACCTGTCTTCTATAATTTAACCATTCTTTTTTACTTGTATATTTTTTCGATGGTTGGAAACCGCTTCCAAAAAACCATAACACGACTCTGCTTCTTCAAGTTCg                                                            <  2:1457254/140‑1 (MQ=255)

                                                                             accACTTTGTACGCATAACCTTAAAAGTACTCGGACAGTTATACTATAAATCAACCCGTCGTTATTACGTGTATATTTTTCCGCTCTTTGGCACCCCCTTCCAAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCa                                                          <  2:2275718/138‑1 (MQ=255)

                                                                             aTCACCTTTTAACCATACCCTAAAAAGTACTCGCACAGTTCTTATATAAATCAACCAGTCTTTATTACCTTTATAATTATTCGATCGTATCCAAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCa                                                          <  2:5011757/140‑1 (MQ=255)

                                                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                     >  1:114130/1‑140 (MQ=255)

                                                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACa                                                     >  1:597503/1‑140 (MQ=255)

                                                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                               <  2:3600481/81‑1 (MQ=255)

                                                                                   atGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                               >  1:3599835/2‑81 (MQ=255)

                                                                                           aTAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGATCGCAAGACAGaacaacat                                            >  2:5777246/1‑138 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                           >  2:2038161/1‑140 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACttt                                           >  2:824743/1‑139 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGAATCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                           >  1:1522803/1‑140 (MQ=255)

                                                                                             acccTTCAAAGTACTCGCACAGGTCTTATATAAATGAACCAGTCTTTATTACCTTTATCATGATTCGATCGTATCCAACCCCTCCCAAAACACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                          <  2:114130/138‑1 (MQ=255)

                                                                                             aaCCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATtga                                          >  2:5527441/1‑140 (MQ=255)

                                                                                               ccTTAAATGTACCCGCACTGTTTTTCTATAAAAGAACCCGTTGTTATTACCTGTTTCATTATTCCTGCGTACGCAAGCCCTGCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                        <  2:4401467/140‑1 (MQ=255)

                                                                                               ccTTAAACGTACTCGCACTGTTTTTCTAAAAATCAACCCGTGTTTTTTACCTTTAACATTTTACTATCGTATCCACCCCCTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                        <  1:5527441/140‑1 (MQ=255)

                                                                                               ccTTAAAATTACTGTCACAGGTCTTATATAAATCAACCCGTGTTTTTTACTTGTATATTTCTTCGATCGTTTGCACGCCCTCCCCAAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                        <  2:445895/140‑1 (MQ=255)

                                                                                               ccTACAAAGTACTCGGACCGGTCTTAAATAAATCAACCAGTCGTTTTTCCCTGTATCATGATTCGATCGTATGAAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                        <  2:3301396/140‑1 (MQ=255)

                                                                                               cattCAAAGTACTCGGACCGGTATTCTATAAATGAACCCGGCGTTATTACCTGTATAATGATTCGATCGTATGCACGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                        <  2:5670093/138‑1 (MQ=255)

                                                                                               cattCAAAGTACCCCTCCCGGTATTCTATAAAACAACCAGTCTTTTTTACCTCTATCTTTATTCGTAGGTTTGCAACCCCTTCCCAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                        <  2:3926403/138‑1 (MQ=255)

                                                                                                    aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                   >  1:272447/1‑140 (MQ=255)

                                                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACATAACAACTTGATGAGGCTTAagag                             >  2:2775508/1‑140 (MQ=255)

                                                                                                              tactggTCTTATATAAATCAACCCGTCTTTATTACCTTTATCATTTTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                         <  2:3563267/136‑1 (MQ=255)

                                                                                                              gacggttcttctACACAGGACCCACGCTTTATACCCGGTATCACTATTCGATCGTCTCAAACCCCTCCCAAAAAACCATACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGATCGAt                         <  2:1824139/134‑1 (MQ=255)

                                                                                                              gACTGGTTTTATATAAATGACCCAGTCGTTTTTACGTTTATCTTGTTTCTCGCGTATGCACGCCCTTCCAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                         <  2:3652262/140‑1 (MQ=255)

                                                                                                              gACTGGTCTTCTATAAATGAACCAGTCTTTTTTACCTGTATCTTGTTTCGATCGTATGCAAGCCCTTCCCAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                         <  2:1041234/140‑1 (MQ=255)

                                                                                                              gACCGGTTTTCTATAAATCAACCATGCTTTTTTACCTTTATCTTGTTTCGATCGTCTGAAACCCGCCCCCAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                         <  2:4757692/140‑1 (MQ=255)

                                                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAAAAACATGATGAGGCTTAAGAGCGATCATg                     >  2:5128169/1‑140 (MQ=255)

                                                                                                                       tatataAATCAACCCGTCTTTATTACCTCTATCATGATTCTATCTTTGGCACCCCTTTCCCAAAACATAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                <  1:1770787/138‑1 (MQ=255)

                                                                                                                        ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGCTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaaa               >  1:3372267/1‑140 (MQ=255)

                                                                                                                         tataAATCAACCAGTTTTTATTACCTTTATCATGTTGGTATGGTATCAAAGCCATCCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt              <  1:4975227/140‑1 (MQ=255)

                                                                                                                                 taCCAGTGTTTATTACTTTTATAATTATTCTATCTTAGGCACGCCCTGCCCAAAACCCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa      <  2:5461874/139‑1 (MQ=255)

                                                                                                                                 aaCCTGTGGTTATTACGTGTATCATTATTCTATCTTATGAAAGCCCTTCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa      <  2:4366690/140‑1 (MQ=255)

                                                                                                                                 aaCCCGTCTTTTTTCCCTGTAACAGTATTCTATCGTCTCCAACCCATTCCAAAACACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa      <  1:1296111/140‑1 (MQ=255)

                                                                                                                                 aaCCAGTCTTTATTACCTTTATAATGATTCTATCGTATGCACGCCCTACCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa      <  1:5440147/140‑1 (MQ=255)

                                                                                                                                  aCCAGTCGTTATTACCTGTATCATGTTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTATTCCAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGGCTAAATTTCCGATAc     >  1:4507675/1‑140 (MQ=255)

                                                                                                                                  aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAACTTGCCGATaa     >  2:2193278/1‑139 (MQ=255)

                                                                                                                                  aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc     >  2:5493254/1‑140 (MQ=255)

                                                                                                                                  aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc     >  1:3892879/1‑140 (MQ=255)

                                                                                                                                     aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc  >  2:60445/1‑140 (MQ=255)

GAAAGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGC  >  NZ_CP034943/3851083‑3851355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`