BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,254	G→C	7.4%	L41V (CTT→GTT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,254	0	G	C	7.4%	690.9 / 3.4	322	L41V (CTT→GTT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (210/72); new base C (4/28); total (222/100)
Fisher's exact test for biased strand distribution p-value = 7.54e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 344 total aligned reads displayed.

CTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851117‑3851393

 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATccc                                                                                                                                          >  2:2675985/1‑140 (MQ=255)

 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                          >  1:2052943/1‑140 (MQ=255)

 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTACCTGTATCATGATTCGATCGTATGAAAGccc                                                                                                                                          >  2:2423070/1‑140 (MQ=255)

 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTAGAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGccc                                                                                                                                          >  1:362684/1‑140 (MQ=255)

      tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                                     >  2:55564/1‑140 (MQ=255)

         ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                  <  2:5429029/140‑1 (MQ=255)

         ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                  <  1:349342/140‑1 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTAGATCGTATTCAAGCCCTTGCCaaaaa                                                                                                                                >  2:1594107/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:463166/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:1958861/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:3012422/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:4131222/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:1913848/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  2:698794/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:747095/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:4443662/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:3645941/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                >  1:981795/1‑140 (MQ=255)

           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAAGCCCGTCCCaaaaa                                                                                                                                >  1:2474918/1‑140 (MQ=255)

            ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTGCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAAc                                                                                                                               >  1:1005281/1‑140 (MQ=255)

            ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAc                                                                                                                               <  2:4236590/140‑1 (MQ=255)

             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                              >  1:4181096/1‑140 (MQ=255)

             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTACCAAAAACg                                                                                                                              >  2:441139/1‑140 (MQ=255)

             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCAAAAACg                                                                                                                              >  1:2087002/1‑140 (MQ=255)

                               ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGcttc                                                                                                            >  2:1484120/1‑140 (MQ=255)

                               ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAACGATAAAACGACTCTGcttc                                                                                                            >  2:900865/1‑140 (MQ=255)

                                           aTCACCTTTTAATCATAACCTTAAAATTACTCGCACAGTTTTTTTATAATTCACCCTGTGTTTTTTACCTTTATAATGATTCTATCGTATGCAAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCa                                                                                                <  2:5331511/140‑1 (MQ=255)

                                                cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                           >  1:114130/1‑140 (MQ=255)

                                                 ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGATATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACAt                                                                                          >  1:407177/1‑140 (MQ=255)

                                                    tAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACCACACTGCTTATTCAAGTTCGCAAGACATaac                                                                                       >  1:5337215/1‑140 (MQ=255)

                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCATTCGTTATTACCAGTATAATGATTCGATCGTAAGCAAGACCATCCCAAAAACGATAAAACGACAATGATTCTTCAAGTTCGCAAGACATAACAACAtg                                                                                 >  1:1421756/1‑140 (MQ=255)

                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGTAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  2:3892907/1‑140 (MQ=255)

                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  2:2589154/1‑140 (MQ=255)

                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  2:420808/1‑140 (MQ=255)

                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:4112979/1‑140 (MQ=255)

                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACAtg                                                                                 >  1:5689201/1‑140 (MQ=255)

                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAAAGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAAAGACTCTGCTTCTTAAAGTTCGAAAGACATAACAACttt                                                                                 >  2:843642/1‑139 (MQ=255)

                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGAATCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:1522803/1‑140 (MQ=255)

                                                             cattCAAAGTACTCGGACCGGTATTCTATAAATGAACCCGGCGTTATTACCTGTATAATGATTCGATCGTATGCACGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:5670093/138‑1 (MQ=255)

                                                             cactCAAAGTACTCGCACAGTTCTTATATAAATCAACCTGTCGTTATTACCTGTATAATTTTTCGATCGTATGCAAGCCCTCCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:141242/137‑1 (MQ=255)

                                                             actacAAATTACTCGCACTGTTAGTCTATAAATTAACCTGTCTTTATTACCTGTAAAATAATTCGATCGTAGGCACGCCCTACCAAAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:2217624/136‑1 (MQ=255)

                                                             acTTCAAACTACTCGCACATTTATTCTAAAAATGAACCAGTGGTTATTACATTTATAATGATTCGATCTTATGCAAGCCCTACCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:3855404/139‑1 (MQ=255)

                                                                 cAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGACGTTATTACCTGTATCATGATTAGATAGTATGCAAGCCCTTACCAAAAACGTTAAAAAGACTCTGCTTCTTAAATTTCGCAAGACATAACAAATTGATGATGc                                                                          >  1:4017443/1‑140 (MQ=255)

                                                                  aaaGTACTCGGACAGGTCTTCTATAAATGTCCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCACGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTGAAAGTTCGCAAGACAAAACCACGTGCGGAGGCt                                                                         >  2:5704761/1‑140 (MQ=255)

                                                                  aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                         >  1:3919426/1‑140 (MQ=255)

                                                                  aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                         >  1:272447/1‑140 (MQ=255)

                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   >  2:1070567/1‑140 (MQ=255)

                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACGTGATGATGCTTAagag                                                                   >  1:5221286/1‑140 (MQ=255)

                                                                            tactggTCTTATATAAATCAACCCGTCTTTATTACCTTTATCATTTTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:3563267/136‑1 (MQ=255)

                                                                            gACTGGTTTTATATAAATGACCCAGTCGTTTTTACGTTTATCTTGTTTCTCGCGTATGCACGCCCTTCCAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:3652262/140‑1 (MQ=255)

                                                                            gACCGGTCTTATATAAATCAACCAGTGGTTTTTACCTTTATATTGTTTCGTACGTAGCCAAGCCCGTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:4017443/140‑1 (MQ=255)

                                                                            caCAGTTATTCTATAAATCAACCATATTTTATTACCTTTATCATGTTTCTATGGTATGCAACCCCTTCCCAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:4534596/139‑1 (MQ=255)

                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAAAAACATGATGAGGCTTAAGAGCGATCATg                                                           >  2:5128169/1‑140 (MQ=255)

                                                                                  tcttctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACATAACAACTTGATTAGGCTTAAGAGCGATCATGTc                                                         >  1:4007322/1‑140 (MQ=255)

                                                                                  tcttctATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAATCACATCCCAAAAACGATAAAACGACTCTACTTATTCAAGTTCGCACGACCTAAAAACTTTATGAGGCATAACATCGCTCATGTc                                                         >  1:3240222/1‑140 (MQ=255)

                                                                                  tcttctATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTc                                                         >  1:238858/1‑140 (MQ=255)

                                                                                      ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGCTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaaa                                                     >  1:3372267/1‑140 (MQ=255)

                                                                                           aaTGAACCAGACGTTATTACCTGTATCAAGATTCGATCGAATGCGAGCCCTTCCCAAAAACGATAAAAAGACTCGGCTTCTTGAAGATCGCAAGACATAACAACGTGATGAGGCTTAAGAGCGATAATCTCTAAATTccc                                                >  1:4330300/1‑140 (MQ=255)

                                                                                               caCCTGTCTTTTTTACGTTTATCTTGATTCGTGCGTTTGAACCCCCGCCCCAAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:5188860/139‑1 (MQ=255)

                                                                                               accccgTCTTTTTTACTTGTATAATGTTTCTATGGTTTGCACCCCCTACCCAAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  1:463166/135‑1 (MQ=255)

                                                                                               aaCCATTCTTTATTACCTTTATATTGATTCGATCGGATGAAAGCCCTCCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:4389185/140‑1 (MQ=255)

                                                                                               aaCCAGTCTTTATTACCTTTATAATGATTCTATCGTATGCACGCCCTACCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  1:5440147/140‑1 (MQ=255)

                                                                                                aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTGTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGTGGCTTAGGAGCGCTCAGGTCTAAATTGCCGagag                                           >  2:2961451/1‑137 (MQ=255)

                                                                                                aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                                           >  2:5493254/1‑140 (MQ=255)

                                                                                                aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCAAGACAGAACAACTTGATGAGGCTTAAGAGCGAACATGTCTAAATTTCCGATAc                                           >  1:4550832/1‑140 (MQ=255)

                                                                                                 ccAGTCGTTATTACCTGGATCATGCTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAAGTGTAAATTGCCGCTACt                                          >  1:592208/1‑140 (MQ=255)

                                                                                                   aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc                                        >  1:2290327/1‑140 (MQ=255)

                                                                                                            taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg                               >  1:236514/1‑140 (MQ=255)

                                                                                                            taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGATCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg                               >  1:3284031/1‑140 (MQ=255)

                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  1:5147240/1‑140 (MQ=255)

                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  1:4385811/1‑140 (MQ=255)

                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAATCATTATAGCCGTTAttt                         >  2:1204527/1‑140 (MQ=255)

                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATAGCCGATACTGCTTATAGCGGTTCttt                         >  2:4319963/1‑140 (MQ=255)

                                                                                                                   aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                        >  1:2573928/1‑140 (MQ=255)

                                                                                                                       tGATTCTATCGTAGTCAACCCCTACCAAAAAACCATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                    <  2:5056827/140‑1 (MQ=255)

                                                                                                                       tGATTCGATCGTATGCAAGCCCTTCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                    <  2:2317659/140‑1 (MQ=255)

                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  1:5526488/1‑140 (MQ=255)

                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  1:484507/1‑140 (MQ=255)

                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  2:2506317/1‑140 (MQ=255)

                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  1:1665911/1‑140 (MQ=255)

                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  2:4721454/1‑140 (MQ=255)

                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  1:3849511/1‑140 (MQ=255)

                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  1:1611832/1‑140 (MQ=255)

                                                                                                                            cGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  1:5030463/1‑140 (MQ=255)

                                                                                                                              aTCTTATGCATGCCCTTCCCAAATCCGATAAAAAGACTCTGCTTCTTCAAGTTCTCAAGACATAACAACTTGATGAGGCTTTAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCTTAAAATCtt             <  2:5781764/140‑1 (MQ=255)

                                                                                                                              aTCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt             >  1:953297/1‑140 (MQ=255)

                                                                                                                                 gTATGCAAGCCCTTCACAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc          <  2:4983719/140‑1 (MQ=255)

                                                                                                                                 gTATGCAAGCCCTACACAAAAACTTTAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACTTCTTGTTGAGGCTTAAGAGCGATCATGTCTATATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc          <  2:5631987/140‑1 (MQ=255)

                                                                                                                                  tATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTcccc         >  2:738444/1‑140 (MQ=255)

                                                                                                                                    ggaacgCCCTCCCCAAACACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTCAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  1:5541924/135‑1 (MQ=255)

                                                                                                                                    ggCACGCCCTACCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  1:2790880/139‑1 (MQ=255)

                                                                                                                                    ggCAAGCCCTTCCCAAAAAAGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  2:5283667/139‑1 (MQ=255)

                                                                                                                                      cAACCCCTTCCCAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  1:3046465/140‑1 (MQ=255)

                                                                                                                                      cAACCCCTCCCAAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  1:1187087/140‑1 (MQ=255)

                                                                                                                                         gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  1:824863/1‑140 (MQ=255)

                                                                                                                                         gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  1:4901516/1‑140 (MQ=255)

                                                                                                                                         gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  1:299612/1‑140 (MQ=255)

                                                                                                                                         gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  2:1788598/1‑140 (MQ=255)

                                                                                                                                         gCCCTTCCAAAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  2:3296688/140‑1 (MQ=255)

                                                                                                                                         gCCCTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  2:2055547/140‑1 (MQ=255)

CTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851117‑3851393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`