BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,262	C→A	11.7%	W38L (TGG→TTG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,262	0	C	A	11.7%	859.2 / 26.5	354	W38L (TGG→TTG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (230/70); new base A (2/52); total (232/122)
Fisher's exact test for biased strand distribution p-value = 1.03e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

TATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGA  >  NZ_CP034943/3851123‑3851398

   ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                       <  1:4915179/140‑1 (MQ=255)

   ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                       <  1:349342/140‑1 (MQ=255)

     atcggAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTAAAATGATTCGAGCGTATTCAAGACCGTCCCaaaaa                                                                                                                                     >  1:3634291/4‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCaaaaa                                                                                                                                     >  1:978280/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                     >  1:4011012/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                     >  1:2538739/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                     >  2:4224300/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                     >  1:2102166/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                     >  1:1913848/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                     >  1:1555220/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                     >  1:4659905/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                       <  2:5660952/138‑1 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                       >  1:5660952/1‑138 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTACCaaaaa                                                                                                                                     >  1:2403691/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAACCCCTTCCCaaaaa                                                                                                                                     >  2:4576489/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGAACGTATGCAAGCCCTTCCCaaaaa                                                                                                                                     >  2:5500617/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGGATTACCTGTATCATTATTCGATCGTAGGCAAGCCCGTCCCaaaaa                                                                                                                                     >  1:76192/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCGTCTATAAATGAACCAGTCGTGATTAACAGTATCATGATTCGATCGTATGCAAGCCCTACCCaaaaa                                                                                                                                     >  1:5719296/1‑140 (MQ=255)

      ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTGCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAAc                                                                                                                                    >  1:1005281/1‑140 (MQ=255)

      ttGGAAATGGAATTCCCTTTTGGCCAGCTTCGTCACCTTGTAAGCATGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAAc                                                                                                                                    <  1:4471610/140‑1 (MQ=255)

       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                                   >  1:4181096/1‑140 (MQ=255)

       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAACg                                                                                                                                   >  1:5375098/1‑140 (MQ=255)

       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                                   >  2:5677487/1‑140 (MQ=255)

       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTACCAAAAACg                                                                                                                                   >  2:441139/1‑140 (MQ=255)

       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCACGCCCTTCCCAACAACg                                                                                                                                   >  1:2317659/1‑140 (MQ=255)

          aaaTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATa                                                                                                                                >  1:672726/1‑140 (MQ=255)

               gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGAAAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGCTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACg                                                                                                                           >  2:1782976/1‑140 (MQ=255)

                         ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAACGATAAAACGACTCTGcttc                                                                                                                 >  2:900865/1‑140 (MQ=255)

                              tatttttATAACATTTTAACAATAACATTCAAATTACTCGCAAAGTTCTTATATAAATCAACCAGTCTTTTTTACCTTTATAATTATTCGATCGTATGCAAGCCCTTCCCAAAAACTATAAAACGACTCTGCTTCTTCaa                                                                                                            <  2:3885128/137‑1 (MQ=255)

                              tatttttACAACCTTTTACGAAAAACCTCAAAATTACTCGCACAGTTATTCTAAAAATCACCCAGCCTTTATAACCTTTAACATCATACCACCGTAGCCACGCCCTGCCAAAAAACGATAAAACGACTCTGCTTCTTCaa                                                                                                            <  2:1087521/137‑1 (MQ=255)

                                   ttATAACTTTTTAAGCATAACGTCAAAATTAGTCGGACCTGTCTTCTATAATTTAACCATTCTTTTTTACTTGTATATTTTTTCGATGGTTGGAAACCGCTTCCAAAAAACCATAACACGACTCTGCTTCTTCAAGTTCg                                                                                                       <  2:1457254/140‑1 (MQ=255)

                                     aTCACCTTTTAACCATACCCTAAAAAGTACTCGCACAGTTCTTATATAAATCAACCAGTCTTTATTACCTTTATAATTATTCGATCGTATCCAAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCa                                                                                                     <  2:5011757/140‑1 (MQ=255)

                                          cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                                >  1:114130/1‑140 (MQ=255)

                                                   aTAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGATCGCAAGACAGaacaacat                                                                                       >  2:5777246/1‑138 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCATCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTtg                                                                                      >  2:831784/1‑140 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                      >  1:4112979/1‑140 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                      >  2:2586822/1‑140 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAACAACAtg                                                                                      >  2:3927816/1‑140 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAAGGTCGCAAGACATAACAACTtg                                                                                      >  2:3240277/1‑140 (MQ=255)

                                                       ccTTAAACGTACTCGCACTGTTTTTCTAAAAATCAACCCGTGTTTTTTACCTTTAACATTTTACTATCGTATCCACCCCCTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                   <  1:5527441/140‑1 (MQ=255)

                                                       ccTTAAAAGTACTCGGACAGGTTTTCTATAAATGAACCAGTCTTTATTACCTTTATCATGATTCGATCTTATGCACGCCCTACCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                   <  2:4103473/140‑1 (MQ=255)

                                                           cAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATTATTAGATCCTATGCAAGACCTTACCAAAAACGATAAAAAGACTATGCTTCTTAAAATTAGCAACACATAACAAATTAATGAGGc                                                                               >  1:3652262/1‑140 (MQ=255)

                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                        >  1:3968640/1‑140 (MQ=255)

                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACGTGATGATGCTTAagag                                                                        >  1:5221286/1‑140 (MQ=255)

                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGAGGGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCAGCAATTTCGAAAGACATAACAACTTGATCAGGATTAacag                                                                        >  1:5182311/1‑140 (MQ=255)

                                                                      taatgttcttctATAAATCAACCTTGTGTTTTTACATTTATATTTTTTAGATCGTATTACAGCCTTGCACAAAAAGTTAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                                    <  2:2268122/134‑1 (MQ=255)

                                                                      gACTGGTTTTATATAAATGACCCAGTCGTTTTTACGTTTATCTTGTTTCTCGCGTATGCACGCCCTTCCAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                                    <  2:3652262/140‑1 (MQ=255)

                                                                      gACTGGTCTTCTATAAATGAACCAGTCTTTTTTACCTGTATCTTGTTTCGATCGTATGCAAGCCCTTCCCAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                                    <  2:1041234/140‑1 (MQ=255)

                                                                          ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAAAAACATGATGAGGCTTAAGAGCGATCATg                                                                >  2:5128169/1‑140 (MQ=255)

                                                                          ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                                >  2:158808/1‑140 (MQ=255)

                                                                            tcttctATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAATCACATCCCAAAAACGATAAAACGACTCTACTTATTCAAGTTCGCACGACCTAAAAACTTTATGAGGCATAACATCGCTCATGTc                                                              >  1:3240222/1‑140 (MQ=255)

                                                                                ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGCTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaaa                                                          >  1:3372267/1‑140 (MQ=255)

                                                                                ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCACGACATAACAACTTGATGAGGCTTTAGAGCGATCATGTCGaaa                                                          >  1:86744/1‑140 (MQ=255)

                                                                                 tataAATGAACCTGTGGTTATTCCCTGTATCATTATTCGATCGTTTGCAAGCCATTCCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                                         <  2:1805257/140‑1 (MQ=255)

                                                                                        taaCCCGTGGTTTTTACCTTTATCTTTATTCGAGCGTATCCAACCCGTCCCAAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                                                  <  1:4084295/139‑1 (MQ=255)

                                                                                        gAACCCTTCGTTTTTACCTGTAAATTTTTTCGATCTTTGGCAAGCCCTTCCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                                                  <  2:2419592/140‑1 (MQ=255)

                                                                                         taCCAGTGTTTATTACTTTTATAATTATTCTATCTTAGGCACGCCCTGCCCAAAACCCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                 <  2:5461874/139‑1 (MQ=255)

                                                                                         aaCCTGTGGTTATTACGTGTATCATTATTCTATCTTATGAAAGCCCTTCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                 <  2:4366690/140‑1 (MQ=255)

                                                                                         aaCCCGTCTTTTTTCCCTGTAACAGTATTCTATCGTCTCCAACCCATTCCAAAACACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                 <  1:1296111/140‑1 (MQ=255)

                                                                                         aaCCAGTCTTTATTACCTGTATCTTTATTCGATCGTATGCACCCCCTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                 <  2:2637864/140‑1 (MQ=255)

                                                                                         aaCCAGTCGTTTTTACCTTTATCTTTATTCGATCGTATGCAAGCCATTCACAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                 <  2:3551610/140‑1 (MQ=255)

                                                                                          aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAACTTGCCGATaa                                                >  2:2193278/1‑139 (MQ=255)

                                                                                          aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAGAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                                                >  2:4300810/1‑140 (MQ=255)

                                                                                           cctttctttattaAGTGTATCATTATTCGATCTTATCCAACCCCTTCAAAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                               <  2:4678484/133‑1 (MQ=255)

                                                                                             aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc                                             >  2:60445/1‑140 (MQ=255)

                                                                                                      taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGATCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg                                    >  1:3284031/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCCAGACATAACAACTTGATGAGGCTTAAGAGCGATCTTGTCTAAATTGCCGATACTGGTTCGAGCGGTTAttt                              >  1:3153874/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                              >  1:4598462/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                              >  1:1472755/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                              >  2:2946019/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                              >  2:5190352/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                              >  1:4385811/1‑140 (MQ=255)

                                                                                                             aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                             >  2:3705369/1‑140 (MQ=255)

                                                                                                              tCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAATAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtttac                            >  1:5120623/1‑138 (MQ=255)

                                                                                                                aTGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCAt                          >  1:2728908/1‑140 (MQ=255)

                                                                                                                 tGATTCGATCTTATGAAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                         <  2:5375098/140‑1 (MQ=255)

                                                                                                                 tGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                         >  2:1309253/1‑140 (MQ=255)

                                                                                                                   aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                       >  1:4862990/1‑140 (MQ=255)

                                                                                                                   aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                       >  2:2506317/1‑140 (MQ=255)

                                                                                                                   aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                       >  1:1665911/1‑140 (MQ=255)

                                                                                                                   aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                       >  1:3849511/1‑140 (MQ=255)

                                                                                                                   aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                       >  1:1611832/1‑140 (MQ=255)

                                                                                                                        aTCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                  >  1:4266124/1‑140 (MQ=255)

                                                                                                                            tATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTcccc              >  2:738444/1‑140 (MQ=255)

                                                                                                                              tgaaaGCCCTACCAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt            <  1:2366037/137‑1 (MQ=255)

                                                                                                                              tGCACGCCATTCACAAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt            <  1:4038272/140‑1 (MQ=255)

                                                                                                                              ggCACGCCCTACCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt            <  1:2790880/139‑1 (MQ=255)

                                                                                                                              ggCAAGCCCTTCCCAAAAAAGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt            <  2:5283667/139‑1 (MQ=255)

                                                                                                                                cacgCCCTTCAAAAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt          <  2:5072586/137‑1 (MQ=255)

                                                                                                                                cAAGCCATCCCCAAAAACGATACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAGTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt          <  2:5620023/140‑1 (MQ=255)

                                                                                                                                aaaccccGTCCCAAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt          <  2:2020019/136‑1 (MQ=255)

                                                                                                                                   gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg       >  2:4503659/1‑140 (MQ=255)

                                                                                                                                   gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg       >  1:5466746/1‑140 (MQ=255)

                                                                                                                                   gCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg       >  1:299612/1‑140 (MQ=255)

                                                                                                                                   gCCCTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg       <  2:2055547/140‑1 (MQ=255)

                                                                                                                                   ccccTCCCCAAAACCCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg       <  2:1623067/139‑1 (MQ=255)

                                                                                                                                      cTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAGTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTACCCCTTCTCAGTTg    >  2:3024168/1‑140 (MQ=255)

                                                                                                                                       ttCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTgg   >  2:4159533/1‑140 (MQ=255)

                                                                                                                                        tCCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa  <  1:2038161/140‑1 (MQ=255)

                                                                                                                                        tCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa  <  1:2586822/140‑1 (MQ=255)

TATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGA  >  NZ_CP034943/3851123‑3851398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`