Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 135,162 C→A 6.0% intergenic (+209/‑29) EO946_RS00715 → / → rpsJ alpha/beta hydrolase/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943135,1620CA 6.0% 1264.1 / 11.5 489intergenic (+209/‑29)EO946_RS00715/rpsJalpha/beta hydrolase/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base C (207/229);  new base A (47/3);  total (256/233)
Fisher's exact test for biased strand distribution p-value = 2.18e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 499 total aligned reads displayed.

AAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATCT  >  NZ_CP034943/135023‑135301
                                                                                                                                           |                                                                                                                                           
   tCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTGTCACGGAGAACTGTCTAACTTg                                                                                                                                          >  2:4532934/1‑140 (MQ=255)
   tCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGGGAGAGGTTGCTGACACACCCGGCCGCGTTGCCAGGGCCAAGGTGTTCAGGTTTTTCTGACGGAGAACTATCTAAAttt                                                                                                                                          >  1:3440203/1‑139 (MQ=255)
       gCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGt                                                                                                                                      >  2:3500927/1‑140 (MQ=255)
           cATACGTCTATTTTCTGTAGAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAAGTGTCTAACTTGATGTAGGc                                                                                                                                  <  1:5166017/140‑1 (MQ=255)
            aTACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTAACGTTTTTCTCACGGAGAACTGTATAACTTGATGTAggag                                                                                                                                 >  2:3034153/1‑138 (MQ=255)
             tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGACCTGTCTAACGTGATGTAGGCGa                                                                                                                                >  2:5016074/1‑140 (MQ=255)
             tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGa                                                                                                                                >  2:904118/1‑140 (MQ=255)
             tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGa                                                                                                                                >  2:4892220/1‑140 (MQ=255)
             tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAGGGCCAAGGTGTTCAGGTTTTTCTCACGGTGAACTGTATAACTGGAGGTAGGGGa                                                                                                                                >  1:1275702/1‑140 (MQ=255)
              aCGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGaa                                                                                                                               <  1:3371039/140‑1 (MQ=255)
              aCGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGaa                                                                                                                               <  1:2237339/140‑1 (MQ=255)
                  cTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAagg                                                                                                                           <  2:4110093/140‑1 (MQ=255)
                  cTATTTTCTGTATAAGGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAagg                                                                                                                           <  1:2334270/140‑1 (MQ=255)
                   tATTTTCTGTCTAATGGGGTATGTGGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGACGCTTTGCCTTGGCAAAGGTGTTCAGGTTTTTCTCAGGGTGAACTGTCTACCTTGTTGTAGGCGAAAagga                                                                                                                          <  2:5213563/140‑1 (MQ=255)
                   tATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCCAGGCAAACGTTTTCAGGTTTTTCTAACGGAAAAATGTAAAAATTTATTTAGGCGAAAaaga                                                                                                                          >  1:4990143/1‑140 (MQ=255)
                   tATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAagga                                                                                                                          <  2:1541586/140‑1 (MQ=255)
                   tATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAagga                                                                                                                          <  2:505567/140‑1 (MQ=255)
                       ttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGTTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGa                                                                                                                      >  2:2445778/1‑140 (MQ=255)
                          tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCATGGCAAATGTGTTCAGGTTTTTATAACGGAGAACTGTCTAAATTTATTTAGGCGAAAAGGAGGGaaaa                                                                                                                   >  1:913689/1‑140 (MQ=255)
                          tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGaaaa                                                                                                                   >  2:2529550/1‑140 (MQ=255)
                          tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCAAGGAGAACTGTAAAACTTGATGTAGGCCAAAAGGAGTGaaaa                                                                                                                   >  2:2201303/1‑140 (MQ=255)
                          tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTAAGGTTTTTCTAACGGAGAAATGTCTAACTTGATGTAGTCGAAAAGTAGGGaaaa                                                                                                                   >  2:3433034/1‑140 (MQ=255)
                            tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGGAAAGGTGTTCAGGTTTTTGTCAAGGAGAACGGTGTAAAATGATTTTGGCGAAAATGAGGGcaaaaa                                                                                                                 >  1:3236199/1‑138 (MQ=255)
                            tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAaata                                                                                                                 >  2:2081045/1‑140 (MQ=255)
                            tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACTGTCTAACTTGATTTAGGCGAAAAGTAGGGAAaata                                                                                                                 >  2:3185609/1‑140 (MQ=255)
                                   gtgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaa                                                                                                          <  1:1023365/140‑1 (MQ=255)
                                    tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCATGGCAAAGGTGTTAAGGTTTTTTTCACGGAGAACAGTCTAAAATTATGTAGGCGAAAAGGCTGGAAAAAAATTGaaaa                                                                                                         >  1:1639859/1‑140 (MQ=255)
                                    tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCAAAGGTGGTGAGGTTTTTCTGACGGAGAACTTTCTAACTTGATTTATGCGAAAAGGAGGGAAAATAAAGTcaaa                                                                                                         >  1:270832/1‑140 (MQ=255)
                                      tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCAGGGCCAAAGTGTTCAGGTTTTTATAAACGATAAATGTCTAAATTGATGTAGGCGAAAAGGAGGGAAAATAAATGcaaaaa                                                                                                       >  1:2111613/1‑139 (MQ=255)
                                      tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATTTAGGCGAAAAGGACGGAAAATAATGGcaaaac                                                                                                       >  2:2163214/1‑140 (MQ=255)
                                      tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaaac                                                                                                       >  2:4664218/1‑140 (MQ=255)
                                      tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAAATGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaaaa                                                                                                       >  2:1994284/1‑139 (MQ=255)
                                           tGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACaaaaa                                                                                                  <  1:3155374/140‑1 (MQ=255)
                                           tGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACaaaaa                                                                                                  <  1:5656908/140‑1 (MQ=255)
                                           tGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAGGGCAATGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACaaaaa                                                                                                  <  1:1150038/140‑1 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATGGCCAACGTGTTGAGGTTTTTTTAACGGGGAACTGTCTAAATTGATTTATGGGAAAAGGAGGGAAAATAATGTCAAAAAAAAAAat                                                                                                >  1:4814285/1‑140 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCGTGCAAAGGTGTTCATGTTTTTTTAACGGCCAACTCTCTAAATTGATGTAGGGGCAAAGGATGGCAAAAAAAATCAaaaaaaaaaaa                                                                                                >  1:5345076/1‑139 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTGTCACGGAGAACTGGATAAATTGATTTAGGCGAAAAGGAGGGAAAATAATGGCAAAAAAAAAAat                                                                                                >  1:2003682/1‑140 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATg                                                                                                                                       <  1:4424125/101‑1 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATg                                                                                                                                       >  2:4424125/1‑101 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATTTAGGCGAAAAGGAGGGAAAATAAAGGCAAAAAAAAAAat                                                                                                >  2:2033432/1‑140 (MQ=255)
                                             gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTGTGCCAGGGCCAAAGTGTTGAGGTTTTTTTCAAGGCGAAATGTGTAAGTTGGTTGTGGCGAAAAGGAGGGGAGATAATGGGAAAACaaaaaaa                                                                                                >  1:474533/1‑139 (MQ=255)
                                              tCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAatt                                                                                               >  2:3260905/1‑140 (MQ=255)
                                                tttGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattag                                                                                             >  2:2734053/1‑138 (MQ=255)
                                                tttGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTAAGGTTTTTCTCACCGAGAACATTCAAAATTTATTTAGGCGAAAAGGAGGGAAAAAAAATGCAAAACAAAAAattat                                                                                             >  2:2915861/1‑138 (MQ=255)
                                                  tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATGGCAAAAGTGTTTAGGTTTTTGTGACGGAGAAAAGTCTAAAATGTTTTATGCGGAAAGGAGGGAAAATAATGGCAAAAAAAAAAaatcgta                                                                                           >  1:782128/1‑140 (MQ=255)
                                                  tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACAGTCTAACTTGATGTATGCGAAAAGGAGGGCAAATAATGGCAAAACAAAAAattagta                                                                                           >  1:3047292/1‑140 (MQ=255)
                                                  tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCATGTTTTTCTCACGGAGAACTGTCTAACTTTGTTTAAGAGAAAAGGAGGGAAAATAAAGTCAAAACAAAAAatttgta                                                                                           >  2:4637847/1‑140 (MQ=255)
                                                  tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTTATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattagta                                                                                           >  2:5357569/1‑140 (MQ=255)
                                                  tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGGGTTCAGGTTTTTCTCACGGAGAACAGTCTAACTTGATGTAGGCGAAACGGAGGGAAAATAATGGCAAAACAAAAAattcgta                                                                                           >  2:3805061/1‑140 (MQ=255)
                                                       gCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTAACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattcgtattcgt                                                                                      >  2:5274319/1‑140 (MQ=255)
                                                        cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAAAAAAAAATTAGTATTCGtt                                                                                     >  2:3575816/1‑140 (MQ=255)
                                                        cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAGGGCAAAGGTGTTGAGGTTTTTGTAACGGAGAACTGTCTAACTTGATTTAGGGGAAAAGGAGGGAAAAAAATGGCAAAACAAAAAAAACGTATTCGtt                                                                                     >  1:4652010/1‑140 (MQ=255)
                                                        cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAAGGCAAAGGTGTTCAGGTTTTTATGACGGGGAACAGTCAACCTTGATGTCGGCGAAAACGAGGGACAATACAGGCAAAACAAAACATTCGTAATCGtt                                                                                     >  1:2952316/1‑140 (MQ=255)
                                                           tGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGa                                                                                  <  2:3989501/140‑1 (MQ=255)
                                                                  agagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCata                                                                           <  1:1739803/140‑1 (MQ=255)
                                                                  agagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCata                                                                           >  2:4328623/1‑140 (MQ=255)
                                                                  agagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCata                                                                           <  2:2599509/140‑1 (MQ=255)
                                                                         gCTGACACACCCGGCCGCTTTGCCATGGGAAACGTGTTCAGGTTTTTATCCCCGAGAACAGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAAATCGGATTCGTTTTAAAGCATATTATCAt                                                                    >  1:2582019/1‑140 (MQ=255)
                                                                          cTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTGTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATa                                                                   <  2:2357885/140‑1 (MQ=255)
                                                                           tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                                                                  <  2:66529/140‑1 (MQ=255)
                                                                                cacCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATcc                                                             <  2:5398600/140‑1 (MQ=255)
                                                                                    cGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTATTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGa                                                         <  1:5493158/140‑1 (MQ=255)
                                                                                    cGGACGCTTTGCCATGGCAAATGTGTTAAGGTTTTTCTCACGGAGAACTTTATAACTTCATGTAGGCGAAAAGGATGGAAAATAATGGCAAAAAAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGa                                                         <  2:5487579/140‑1 (MQ=255)
                                                                                      gCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATc                                                       <  2:1391963/140‑1 (MQ=255)
                                                                                       ccGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCa                                                      <  1:4532226/140‑1 (MQ=255)
                                                                                       ccGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCa                                                      <  1:4328623/140‑1 (MQ=255)
                                                                                         gCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAAt                                                    <  1:1211509/140‑1 (MQ=255)
                                                                                           tttGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCt                                                  >  2:1269589/1‑140 (MQ=255)
                                                                                              gCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCa                                               <  1:707490/140‑1 (MQ=255)
                                                                                                  tGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAgaga                                           >  1:2197251/1‑140 (MQ=255)
                                                                                                     cAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGagaaga                                        >  2:3100640/1‑140 (MQ=255)
                                                                                                         ggTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGAttgt                                    >  1:3886569/1‑140 (MQ=255)
                                                                                                            gtTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCACTCTGCAGAGAAGATTGTTGa                                 >  1:5712042/1‑140 (MQ=255)
                                                                                                            gtTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGa                                 <  2:1121842/140‑1 (MQ=255)
                                                                                                             ttCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGaa                                >  1:3957443/1‑140 (MQ=255)
                                                                                                             ttCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGaa                                >  2:1642201/1‑140 (MQ=255)
                                                                                                                   tttttCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCa                          <  2:1813130/140‑1 (MQ=255)
                                                                                                                   tttgtCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCACACCAAAAAATTCGTCTTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCa                          <  1:4249527/140‑1 (MQ=255)
                                                                                                                     tttCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCaaa                        <  1:1425335/140‑1 (MQ=255)
                                                                                                                       tctcACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAAc                      >  1:4079085/1‑140 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  2:5701391/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:3593237/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:156770/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:5224801/140‑1 (MQ=255)
                                                                                                                           aCGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTc                  <  1:3500927/140‑1 (MQ=255)
                                                                                                                            cGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCt                 >  1:2068500/1‑140 (MQ=255)
                                                                                                                               agaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGt              <  2:773785/140‑1 (MQ=255)
                                                                                                                               agaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGGTCTGGt              <  1:2917169/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  2:4702565/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  2:1880560/140‑1 (MQ=255)
                                                                                                                                gaACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTg             <  1:5453637/140‑1 (MQ=255)
                                                                                                                                    tGTCTAACTTGAGGGAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAg         <  2:4452434/140‑1 (MQ=255)
                                                                                                                                     gTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAgt        <  2:1498328/140‑1 (MQ=255)
                                                                                                                                     gTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAgt        <  1:5274319/140‑1 (MQ=255)
                                                                                                                                     gTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAgt        <  2:2987430/140‑1 (MQ=255)
                                                                                                                                      tCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAgtg       >  1:1910517/1‑140 (MQ=255)
                                                                                                                                          aCTTGATGGAGGCGAAAAGGATGGAAAATAATGTCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATc   <  2:3341367/140‑1 (MQ=255)
                                                                                                                                           cTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATCt  >  1:2422330/1‑140 (MQ=255)
                                                                                                                                           cTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATCt  >  1:3770936/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCAGAGAAGATTGTTGAAACGGCAAAACGTTCTGGTGCTAGTGTATCT  >  NZ_CP034943/135023‑135301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: