Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,976,149 G→T 5.3% G19G (GGC→GGA rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,976,1490GT 5.3% 1010.7 / 4.9 385G19G (GGC→GGArlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base G (125/222);  new base T (35/2);  total (161/224)
Fisher's exact test for biased strand distribution p-value = 1.24e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 387 total aligned reads displayed.

GGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATC  >  NZ_CP034943/3976011‑3976287
                                                                                                                                          |                                                                                                                                          
ggATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGc                                                                                                                                           <  2:1936052/140‑1 (MQ=255)
 gATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGcc                                                                                                                                          <  1:3699113/140‑1 (MQ=255)
 gATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGcc                                                                                                                                          <  2:5053774/140‑1 (MQ=255)
 gATAGGATGCGGTCGCCTTCTTTGGCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTGTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGcc                                                                                                                                          <  2:857427/140‑1 (MQ=255)
    aGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGAGTTTTGCGTATGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttg                                                                                                                                       >  2:4959786/1‑140 (MQ=255)
     ggATTCGGTCGGCGTCGGTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTCGTGCGGGGGTTGGATGGTGTGGATTTTGGCGTTGGCCGATATGCGTTGGGTGTTTTCTTCAATGcgggtg                                                                                                                                      >  1:2042132/1‑135 (MQ=255)
     ggATGCGGTCGCCTTCTTTGTCTTGTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTACGGGCGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTAAATGCcttgc                                                                                                                                      >  2:761651/1‑140 (MQ=255)
      gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTAGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGGGTATTCTTCAATGCcgtggt                                                                                                                                     >  1:3915896/1‑138 (MQ=255)
      gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTGGTGCGGGAGTTAGATGATGTCGATTTTTTCGTAGGCCCAAACTCGTTTGGGGTATTGTTAAATGCcgtggg                                                                                                                                     >  1:2367441/1‑138 (MQ=255)
      gATGCGGTCGCCTTCTTTGGCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCGTTTTCGTCCGGGAGTTCGATGGTGTCGATTTTTGGGTAGGCCGAAAGTGGTTTGGTGTATTCTTCAATTCcttgct                                                                                                                                     >  1:4750921/1‑140 (MQ=255)
           ggTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCAGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTGTTCAATGCGATGCTTGAgg                                                                                                                                >  2:3414536/1‑140 (MQ=255)
            gTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTAGTCCGGGAGTTAGATTATGTAGATTTTTTCGTAGGCCGAAATTCGTTTGGGGTATTGTTAAATGGCTTGCTTGAGGt                                                                                                                               >  1:945668/1‑140 (MQ=255)
               gCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTAtt                                                                                                                            <  2:5486538/140‑1 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGACTTGCTTGAGGTAttt                                                                                                                           >  2:3877517/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATGCATTGCTTGAGGTAttt                                                                                                                           >  2:3306388/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGGACGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCCAAAGTGGTTTAGTGTATTAATCAATTCCTTGCTTGAGGTAttt                                                                                                                           >  1:5433377/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATTCCTTGCTTGAGGTAttt                                                                                                                           >  1:4280750/1‑140 (MQ=255)
                acttcttTGTCTTTTATGATTTACATGTCCTGGTCGCTTATCTATTCCGGCGCTTTATACTACGGGAGTTCGAAGATGTCGAATTTTTAGTATTCCGCAATAAGATTAGAGTATTCTTAAATGCAGTGTTTGACGTaatg                                                                                                                           >  2:485128/2‑137 (MQ=255)
                  ttcttTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCct                                                                                                                                         <  1:5084578/124‑1 (MQ=255)
                    cttTGTCTTTTATGATTTTCATGTCCTGGTCTCTTAGGGTTTCACGCCCTTTTTCGTACGGGCCATAGATTATTTCGATTTTTTCGTAGGGACAAAGTACTTTGGTTTATTAATAAAATCCATGGATGAAGTAttttttt                                                                                                                       >  1:4932722/1‑138 (MQ=255)
                       tGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTAGATTTTTTGGGAGGCCGAAAATCGGTTCGTGTATTCTTAAATGCCTTGGGTGGGGTATTTTTCtttt                                                                                                                    >  1:2539777/1‑140 (MQ=255)
                         tCTTTTATGATTTTCATGTCCTGGTCGCTTAGGGTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTATGCCGAAAGTCGTTTAGTGTATTCTTCAAAGCCATGCTTTTGGTATTTTTCTTTTat                                                                                                                  >  1:2607686/1‑139 (MQ=255)
                           ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAGGTATTTTTCTTTTAGtt                                                                                                                >  2:1328157/1‑140 (MQ=255)
                              tATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGcc                                                                                                                                          >  2:5674826/1‑111 (MQ=255)
                              tATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGtttta                                                                                                             >  2:577613/1‑139 (MQ=255)
                              tATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGGCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTc                                                                                                             >  2:4190608/1‑140 (MQ=255)
                                   ttttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAtt                                                                                                        <  1:2268889/140‑1 (MQ=255)
                                   ttttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCGTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTACGAtt                                                                                                        >  2:1671361/1‑140 (MQ=255)
                                   ttttCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTATGCCGAAAGTCGTTTCGTGTATTATTCAAATCCGTGCTTGAGGTATTTTTATTTTAGTTTTACGAtt                                                                                                        >  1:1774756/1‑140 (MQ=255)
                                     ttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTGGTTTCGGGTATTCTTCAATGCCTTGCTTGGTGTATTTTTCTTTTAGTTTTACGAtttt                                                                                                      >  2:2672305/1‑138 (MQ=255)
                                     ttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                      <  1:2437824/140‑1 (MQ=255)
                                     ttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTCTCCGATTGt                                                                                                      <  1:444655/140‑1 (MQ=255)
                                       cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                    <  2:5189036/140‑1 (MQ=255)
                                          gTCCTTGTCGCTTAGGTTTTGCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTAGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGACTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACaa                                                                                                 <  1:1692047/140‑1 (MQ=255)
                                          gTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACaa                                                                                                 <  1:776827/140‑1 (MQ=255)
                                            ccTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAata                                                                                               <  1:300245/140‑1 (MQ=255)
                                            ccTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAata                                                                                               <  1:2735257/140‑1 (MQ=255)
                                               ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:2246963/140‑1 (MQ=255)
                                               ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:3627436/140‑1 (MQ=255)
                                                gTCGCTTATGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATTTCGATTTTTTCGTAGGCCGAAAGTCGTTTAGTGTATTATTCAATTCCTTGGTTTAGGTATTTTTGTTTTAGTTTTACGATTTTAACaaaaatta                                                                                           >  1:4609274/1‑134 (MQ=255)
                                                     ttAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGCTGATGTCGATTTTTGCGTAGGCCGAAAGTAGTTTCGTGTATTCTTCAAATCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTTTCACACTATTGATAtta                                                                                      >  2:2028498/1‑139 (MQ=255)
                                                     ttAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATTCCTTGCTTGAGGTATTTTTCTTTTATTTTTCCGATTGTCACAAAATTGATAtta                                                                                      >  2:4237791/1‑139 (MQ=255)
                                                      tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                                                     <  1:2313042/140‑1 (MQ=255)
                                                             tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCTATTGTCAAAATATTGATATTCACAGGTCa                                                                              >  2:4144324/1‑140 (MQ=255)
                                                             tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACATGTCa                                                                              >  2:5472902/1‑140 (MQ=255)
                                                             tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  1:1338147/1‑140 (MQ=255)
                                                             tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCGTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  2:2193387/1‑140 (MQ=255)
                                                                 gcgcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATccc                                                                          <  2:4989911/140‑1 (MQ=255)
                                                                   gcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCa                                                                        >  2:5300599/1‑140 (MQ=255)
                                                                   gcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCa                                                                        >  1:3552950/1‑140 (MQ=255)
                                                                             ccGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAAc                                                              <  1:1289457/140‑1 (MQ=255)
                                                                                ggAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                           <  1:4218747/140‑1 (MQ=255)
                                                                                 gAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGt                                                          <  2:5102065/140‑1 (MQ=255)
                                                                                  aGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGtt                                                         <  2:2550837/140‑1 (MQ=255)
                                                                                  aGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGtt                                                         <  2:58066/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:5582967/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:3491957/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:1401262/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:4356637/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:1573953/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:4043491/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:3908307/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTAGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:4475223/140‑1 (MQ=255)
                                                                                   gTTCGATGAGGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGTGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:2301661/140‑1 (MQ=255)
                                                                                   gTTCGAGGCTGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:3802883/140‑1 (MQ=255)
                                                                                     tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  1:1880042/140‑1 (MQ=255)
                                                                                     tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  2:3552950/140‑1 (MQ=255)
                                                                                     tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  2:2383450/140‑1 (MQ=255)
                                                                                       gatgatGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTGTTCAATTCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCa                                                    >  2:4565852/1‑140 (MQ=255)
                                                                                            tgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatatat                                               >  2:3196466/1‑140 (MQ=255)
                                                                                            tgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatatat                                               >  2:1085272/1‑140 (MQ=255)
                                                                                             gTCTATTTTTGCGTAGGACGATAGTCGTTTCGTGTATTCTTCACTGCCTTGCTTGAGGTATTTTTCTTTTATTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:3789286/140‑1 (MQ=255)
                                                                                             gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  2:5524073/140‑1 (MQ=255)
                                                                                             gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:2122199/140‑1 (MQ=255)
                                                                                             gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  2:1754108/140‑1 (MQ=255)
                                                                                             gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  2:1197558/140‑1 (MQ=255)
                                                                                             gTCGATTGTTGCGGAGTCCGAAAGTCGTTTCGTGTATTATTCACTGCCGTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCCCAATATGGATATTCACTGGGCATCCCCACTTTAAAAACAAGTGATTCatctctg                                              <  2:1570224/140‑6 (MQ=255)
                                                                                               cGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtgtt                                            <  2:2094288/140‑1 (MQ=255)
                                                                                                  tttttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTc                                         <  1:5587218/140‑1 (MQ=255)
                                                                                                       gagTAGGCCTAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAACAACAAGTTATTCATATATGTTGTCCACaa                                    <  2:3252968/138‑1 (MQ=255)
                                                                                                        cGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAAt                                   >  1:2831933/1‑140 (MQ=255)
                                                                                                                 aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  1:3275827/140‑1 (MQ=255)
                                                                                                                 aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  1:3085401/140‑1 (MQ=255)
                                                                                                                 aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  1:2811438/140‑1 (MQ=255)
                                                                                                                   aGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCt                        <  2:3755295/140‑1 (MQ=255)
                                                                                                                       gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTc                    >  2:691976/1‑140 (MQ=255)
                                                                                                                        tttCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCt                   >  2:1694355/1‑140 (MQ=255)
                                                                                                                        tttCGTGTATTCGTCAATGCCTTGCTTGAGGTATTTTTCTTTGAGTTTTCCGATTGTCACAATATTGATAGTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCt                   <  2:3140103/140‑1 (MQ=255)
                                                                                                                        tttCGTGTAATCTTAAATGCCTTGCTTGAGGTATTTATCTTTTAGTTTTCCGAATGTCACAATATTTATATTCACAGGTCATCCCCACTTTAAAAACAACTTATTAAAATATGATGTCCACAATTGTGGAtatcttatct                   >  1:3953508/1‑140 (MQ=255)
                                                                                                                             tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  1:4496253/140‑1 (MQ=255)
                                                                                                                             tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:4708281/140‑1 (MQ=255)
                                                                                                                             tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:4886947/140‑1 (MQ=255)
                                                                                                                             tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:3029965/140‑1 (MQ=255)
                                                                                                                                   cttcAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTgtgt        <  1:4806845/140‑1 (MQ=255)
                                                                                                                                   cttcAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTgtgt        <  1:3224724/140‑1 (MQ=255)
                                                                                                                                   cttcAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTgtgt        <  1:5710762/140‑1 (MQ=255)
                                                                                                                                      cAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAg     <  1:714466/140‑1 (MQ=255)
                                                                                                                                      cAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAg     <  2:2902543/140‑1 (MQ=255)
                                                                                                                                         tGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGTTc  >  1:5226168/1‑140 (MQ=255)
                                                                                                                                         tGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATc  >  1:1065454/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATC  >  NZ_CP034943/3976011‑3976287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: