Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,857 C→A 5.9% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8570CA 5.9% 739.0 / 2.9 291intergenic (‑163/+43)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base C (126/135);  new base A (2/26);  total (128/163)
Fisher's exact test for biased strand distribution p-value = 1.17e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCT  >  NZ_CP034943/3996718‑3996994
                                                                                                                                           |                                                                                                                                         
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGACACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCAGTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:5662794/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:545286/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:5536167/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:322536/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  2:1915099/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAACTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  2:3344715/1‑140 (MQ=255)
       gATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTaaaaat                                                                                                                                    >  1:3769385/1‑139 (MQ=255)
         ttCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAATGCt                                                                                                                                  >  2:2883684/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGACAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  1:1316209/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  2:812854/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  2:1142437/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  1:3625008/140‑1 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCACTATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCACCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                        <  2:1859693/140‑1 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                        >  1:2764330/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGATCGTGAAACtt                                                                                                                        >  2:4636112/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTACATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                        <  2:299565/140‑1 (MQ=255)
                     aaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtttt                                                                                                                      <  2:4758117/140‑1 (MQ=255)
                      aaTCAAGTTTATCAGTTAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                     <  2:1201400/140‑1 (MQ=255)
                      aaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                     <  2:3893818/140‑1 (MQ=255)
                      aaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                     <  2:3458855/140‑1 (MQ=255)
                       aTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTAt                                                                                                                    >  2:5176/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                   >  2:1947949/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                   >  1:3086089/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAAGCAATATTCGTTCGCTTCCATTCGGTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                   >  2:3480645/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                            >  2:2547113/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                            <  1:1628819/140‑1 (MQ=255)
                                 tCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGTAc                                                                                                          >  2:1398532/1‑140 (MQ=255)
                                     tGAAGAAACATAAAGTCACTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                      <  1:664551/140‑1 (MQ=255)
                                     tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                      <  1:1952320/140‑1 (MQ=255)
                                     tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                      <  1:4119547/140‑1 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                    >  1:3401921/1‑140 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                    >  1:2655338/1‑140 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGAGTTTTATCATCGCATTGTGCTAAAAAGCTATTGAAACTTTTATCCTTAAGCACGaaaaa                                                                                                    >  1:4923963/1‑140 (MQ=255)
                                               gAAACTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGGTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                            <  1:5271386/140‑1 (MQ=255)
                                                 aaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTg                                                                                          <  1:3832010/140‑1 (MQ=255)
                                                      aaTTTTAAATCCGAACATTAAATTAATGTACCTCAATATTCGTTCGCGTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                     <  1:1108300/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                  <  2:5464934/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                  <  2:3857142/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                  <  2:361389/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                  <  2:3139900/140‑1 (MQ=255)
                                                          gTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATc                                                                                 >  2:1430884/1‑140 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCtt                                                                                                                <  2:272499/105‑1 (MQ=255)
                                                                  gAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTTATATAACTTTTGt                                                                         >  1:1220775/1‑140 (MQ=255)
                                                                  gAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGt                                                                         >  1:4704968/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  1:2338492/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTGTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  1:228351/1‑140 (MQ=255)
                                                                               aTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATCTCCCTTTTGTATTCATAAAAACg                                                            >  2:1685970/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  1:4676159/74‑1 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCTCGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:1011440/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:5100719/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:5365468/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:4418414/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:4358720/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:2707803/1‑140 (MQ=255)
                                                                                        aaTATTCGTTCGCTTCCATTCTTTTTAAAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTAACTTGCCTGATATCCCTTTTGTTTTAATAAAAACGACTCGCCaa                                                   <  2:2610395/140‑1 (MQ=255)
                                                                                        aaTATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCaa                                                   >  1:2173116/1‑140 (MQ=255)
                                                                                         atatTCGGTCGCTCCCATTCGTTTAACATGCTTTTTCACATCCATTTGTCATAAAAAGATCTTTAAAATTTTAACCTTTCGCACTAAAACGAGTTAGTTGCCTCATATCCCTTTTGTATTCATAAAAACGACTCGCCAAc                                                  <  2:4541503/140‑1 (MQ=255)
                                                                                          tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACTACTCGCCAAcc                                                 >  2:2219464/1‑140 (MQ=255)
                                                                                             tggttcgCTTCCTTTGGTTTTAAAAGCGTTTTATCATCGCTTTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGTGTTAGTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTc                                              <  2:1838058/138‑1 (MQ=255)
                                                                                             tctttcgGTTCCATTCTTTTTACAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATAGCCCTTTTGTATTAAAAAAAACGACTCGCCAACCGTc                                              <  2:4704968/137‑1 (MQ=255)
                                                                                                ttcgTTTACATTCGTTTTAAACGGTTTTTCTAATCGAATTGTCCTAAAAAGCCCGTGAAACTTTTATCCTTTCGCACTAAAAAGATTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                                           <  2:5451441/140‑1 (MQ=255)
                                                                                                ttcgCTTCCTTTCTTTTTAAAACCTTTTTCTCATCGAATTTGCATAAAACGCTCGTCAAAGTTTATTCCTTTAGCCCGAAAAAGAGTTACGTCCCTGATATCCCTTTTTTTTTCAAAACAACGACTCGCCAACCGTCCgg                                           <  2:43726/140‑1 (MQ=255)
                                                                                                ttcgCTTCAATTCTTTTTACATGGTTTTTATCATCGATTTGTCCTAAAAAGCTCGTGAAAATTTTACCCTTTAGCATTAAAAATAGTTATTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                           <  2:1033124/140‑1 (MQ=255)
                                                                                                ttcgCTCCCATTCTTTTTACAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTGAAATTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                           <  2:2460272/140‑1 (MQ=255)
                                                                                                ttcgATTCCATTCGTTTTACACGGGTTTTCTCATCGCATTGTCCTAAAAAGCTGGTTAAATTTTTATCATTTAGCACGAAAAAGATTTACTTGCCTGATATCCGTTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                                           <  2:3024207/140‑1 (MQ=255)
                                                                                                 tcgGTTCCTTTGTTTTTACATGCGTTTTCACATCGTATTTCCAAAAAAAGCTCTTCAAAGTTTTATCATTTCCCACTAAAAAGTTTTATTTGCCTGATTTCCCTTTTGTTTAAATAAACACGACTCGCCAACCGTCCGGa                                          <  1:5704100/140‑1 (MQ=255)
                                                                                                 tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGa                                          >  1:2539425/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                     <  2:2051860/94‑1 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                       >  1:1921710/1‑140 (MQ=255)
                                                                                                            ggTTTTAAAAGGGTTTTATCATCGCATTGTCCTAAAAAGCTCGTGAAAATTTTATCCTTTAGCACGAAAAAGAGTTACTTGCGTTATACCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGt                               <  2:1343499/139‑1 (MQ=255)
                                                                                                            cGTTTTACAAGTTTTTTGACAGCGATTTTTAATAAAAAGCTCGTTAATATTTTATCCGTTCGAACTAAAAATAGTTACTGGCCTTATATCCCTTTTTTTTACAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGt                               <  1:3321250/140‑1 (MQ=255)
                                                                                                            cGTTTTACAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGATCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt                               <  2:4102781/140‑1 (MQ=255)
                                                                                                              ttttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGACCGGATCCTCTATCGTCa                             <  2:1103551/140‑1 (MQ=255)
                                                                                                               tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                            >  1:4292264/1‑140 (MQ=255)
                                                                                                               tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                            >  2:755689/1‑140 (MQ=255)
                                                                                                               tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                            >  2:3228822/1‑140 (MQ=255)
                                                                                                                ttACATGTGTTTACTCATGGCTTTGTCATAAAAAGCTCTTGAACCTTTTATCCTTTTGAACTAAAATGTGTTCGTTGCCTGATATCCATTTTGTATTCTTAAAAACGACTCGCCAAACGTCCGGATCCTCTATCGTCACg                           <  2:5648877/140‑1 (MQ=255)
                                                                                                                ttACACGCGTTTTCTCACCGCTTTGTCCTAAAAAGCTCTTTAAACTTTTATCCGTTAGCACCAAACAGAGTGACTTGCCTGATATCCGTTTTGTTTTAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                           <  1:2461390/140‑1 (MQ=255)
                                                                                                                ttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCAGGATCCTCTATCGTCAcc                           >  2:5767886/1‑139 (MQ=255)
                                                                                                                ttAAAAGCTTTTTCTCATCGCATTTTCATAAAAAGCTCGTTAAACTTTTAGCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                           <  2:4253688/140‑1 (MQ=255)
                                                                                                                  aCAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAGCGTCCGGATCCTCTATCGTCACGcc                         >  2:1250986/1‑140 (MQ=255)
                                                                                                                  aCAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGcc                         >  2:2573862/1‑140 (MQ=255)
                                                                                                                  aCAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAACGTCCGGATCCTCTATCGTCACGcc                         >  1:5170421/1‑140 (MQ=255)
                                                                                                                      gttttttCTCTTCGCTTTGTCCTAAAAAGCTCGTTAAAATTTTATCCTTTACCACGAAAATGATTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                     <  2:1458633/137‑1 (MQ=255)
                                                                                                                      gctgttactcAGCCCATGGTCCAAAAAACCTCCTCAAACTTTTATCCCGACCCACAAAAAAGTTTTAGTTGCCTGATAACCCTTTTGTATTCAAACACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                     <  1:3942012/133‑1 (MQ=255)
                                                                                                                       gtttttATCATCGATTTTTCCTAAAAAGCTGGTTAAACTTTTATCATTTACCACCAAAAATAGTTACTTGCCTTATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:2282890/138‑1 (MQ=255)
                                                                                                                       ggTTTTCTCTTCGCATTTTCCTAAAAAGCTCGTTAAACTTTTATCATTTAGCACGAAAAAGAGTTATTTGCATTATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:3743751/139‑1 (MQ=255)
                                                                                                                       ggTTTTCTCTTCGCATTGTCCTAAAAAGCTCTTTAAACTTTTATCCTTTAGCACGAAAATGTGTTACTTGCCTGATATCCCTTTTGTTTTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:5097251/139‑1 (MQ=255)
                                                                                                                       ctttttCTAATCGATTTTTCATAAAAAGCTCTTTAAATTTTTTTCCTTTACCACTAAAAATTTTTTGTTGCTTGATATCCCTTTTTTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  1:4625966/138‑1 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                  >  1:1799860/1‑140 (MQ=255)
                                                                                                                          tttCTCATCGCATTGTCCTAAAAAGCTCTTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCATTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                 <  2:4846877/140‑1 (MQ=255)
                                                                                                                          tttATCATGGTATTGTCCAAAAAAGCTGTTTATATTTTTTTCTTTTAGCACCAAAAATATTAATTGGCCATATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCGGGATCCTCTATCGTCACGCCTCCATGGc                 <  2:4923963/140‑1 (MQ=255)
                                                                                                                           ttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCt                >  1:1185492/1‑140 (MQ=255)
                                                                                                                            tcacctCGCATTGTCCTAAAAAGCCCGTCAAACTTTTAGCCTTTACAACGAAAACGAGGTACTGGCCTGATATCCGTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTc               <  2:554354/135‑1 (MQ=255)
                                                                                                                                 ccGCATGGTCCTAAACAGCGCGTCAAACTTTTATCCTTTAGCACGAAAAAGTGTTACTTGCCTGATCTCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa          <  2:2517803/139‑1 (MQ=255)
                                                                                                                                  cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc         >  1:393595/1‑140 (MQ=255)
                                                                                                                                  agctttGTCCTAAAAAGCTCGTTAAATTTTTATCCTTTAGCACTAAAATGAGTTAGTTGCCTGATATCCCTTTTTTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc         <  2:3803429/136‑1 (MQ=255)
                                                                                                                                    cATTGCCCAAAACCGCTCGCCAAAATTTTATCCGTAACTAACAAACAGTGTTAGTGGCGTGATATCCCTTTTGTATGAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACgg       <  1:1398532/140‑1 (MQ=255)
                                                                                                                                         tCCTAAAAAGCTGGTGAAACTTTTATCCTTTCCCACCAAAAAGAGTTAGTGGCCTGATATCCCTTTTGTATACAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct  <  1:1749646/140‑1 (MQ=255)
                                                                                                                                         tCCTAAAAAGATCGTTAAATTTTTATCCTTTCGCACGAAAAAGAGTTAGTTGCCTTATATCCCTTTGGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct  <  2:1284503/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCT  >  NZ_CP034943/3996718‑3996994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: