BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,996,910	G→T	7.5%	S137* (TCA→TAA)	EO946_RS20530 ←	VOC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,996,910	0	G	T	7.5%	855.1 / 4.6	386	S137* (TCA→TAA)	EO946_RS20530	VOC family protein
Reads supporting (aligned to +/- strand): ref base G (162/164); new base T (2/47); total (164/222)
Fisher's exact test for biased strand distribution p-value = 5.42e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 395 total aligned reads displayed.

AATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAG  >  NZ_CP034943/3996772‑3997049

 aTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGat                                                                                                                                           <  1:5026801/140‑1 (MQ=255)

  ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                                                                          >  2:3589004/1‑140 (MQ=255)

    gTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATc                                                                                                                                        >  2:2572194/1‑140 (MQ=255)

        aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                                                                                    <  2:5016125/140‑1 (MQ=255)

             aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                                                                               <  2:3086089/140‑1 (MQ=255)

             aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                                                                               >  2:1440960/1‑140 (MQ=255)

                    aattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCGTTAGAACGAAAAAGAGTTACTTGCCGGATATCCCTTTTGTATTCATaa                                                                                                                        >  1:5618228/1‑140 (MQ=255)

                        aaTTTAAATCAATTGTCGTTCGGTTCCTTTGTTTTTACAAGCGTTTTCTCATCGCTTTTTCCTAAAAACCTCGTGAAAGTTTTATCGTTGAGCACGAAAAAGATTTACTTGCCTTATATCCCTTTTTTATTCATAAAAAc                                                                                                                    <  2:836481/140‑1 (MQ=255)

                         aTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATCTCCCTTTTGTATTCATAAAAACg                                                                                                                   >  2:1685970/1‑140 (MQ=255)

                         aTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACg                                                                                                                   >  1:1103551/1‑140 (MQ=255)

                          tGTAATTCAATCTCCGTTGGCTTAGATTCGTTTTACAAGCGTTTTCTCAGCGCATGGTCCTAAAATGATCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGGTGCCTGATATCCCTTTTGTTTTAATAAAAACGa                                                                                                                  <  2:3010299/140‑1 (MQ=255)

                          tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTATCACGAAAAAGAGTTACTTGACTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                  >  1:3779996/1‑140 (MQ=255)

                          tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCTCGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                  >  1:1011440/1‑140 (MQ=255)

                          tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                  >  1:5365468/1‑140 (MQ=255)

                          tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                  >  1:2681538/1‑140 (MQ=255)

                          tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                  >  1:784495/1‑140 (MQ=255)

                             aaaTCAATAGTCGTTCGCTTCCTTTCTTTTTACAAGCGTTTTCTCATCGAATTTTCCTAAAAAGCTCGTGAAAGTTTTATCGTTTAGCACGAAAAAGAGTTAGTTGCCTTTTTTCCCTTTTTTTTTAATAAAAACGACTc                                                                                                               <  2:1877584/140‑1 (MQ=255)

                              aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                                                                              >  1:5203158/1‑140 (MQ=255)

                                  aaTATTCGTTCGCTTCCATTCTTTTTAAAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTAACTTGCCTGATATCCCTTTTGTTTTAATAAAAACGACTCGCCaa                                                                                                          <  2:2610395/140‑1 (MQ=255)

                                   atatTCGGTCGCTCCCATTCGTTTAACATGCTTTTTCACATCCATTTGTCATAAAAAGATCTTTAAAATTTTAACCTTTCGCACTAAAACGAGTTAGTTGCCTCATATCCCTTTTGTATTCATAAAAACGACTCGCCAAc                                                                                                         <  2:4541503/140‑1 (MQ=255)

                                    tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAAcc                                                                                                        >  2:4878275/1‑140 (MQ=255)

                                       tctttcgGTTCCATTCTTTTTACAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATAGCCCTTTTGTATTAAAAAAAACGACTCGCCAACCGTc                                                                                                     <  2:4704968/137‑1 (MQ=255)

                                          ttcgTTTACATTCGTTTTAAACGGTTTTTCTAATCGAATTGTCCTAAAAAGCCCGTGAAACTTTTATCCTTTCGCACTAAAAAGATTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                                                                                                  <  2:5451441/140‑1 (MQ=255)

                                          ttcgCTTACTTTGGTTTTAAATGGTTTTTCTCCTGGATTTTTCATAAAACGCTCGTTAAATTTTTATCCTTCAGCACTAAAAATAGTTAGTGGCCTGATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCgg                                                                                                  <  1:2463022/140‑1 (MQ=255)

                                          ttcgCTGCAATTCTTTTTAAAAGCGTTTTCTAAGCGCATTGTCATAAAAACCTCGTTAAAATTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                                                                                                  <  2:1076996/140‑1 (MQ=255)

                                           tcgTTTCAATTATTTTTACACGCTTTTTCTCATCGCTTTTTCCTAAAAAGCTGGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTAGTCATAAAAACGACTCGCCAACCGTCCGGa                                                                                                 <  2:2482583/140‑1 (MQ=255)

                                           tcgCTTCCATGCTTTTTACACGCGTTTTCTCAGCGAATTGTCCTAAAAAGCTCGTTAAAGTTTTATCCTTTAGCACGAAAATGAGTAACTGGCCTGATATCCCTTTTGTATACATAAAAACGACTCGCCAACCGTCCGGa                                                                                                 <  2:3463212/140‑1 (MQ=255)

                                             gatcccATTGGTTTTACAAGGTTTTACACATCGCTTTGTCCAAAAAACCGCTTCAAAATTTTATCCTTTACCACCAAAAAGATTTCCTTGCCTTATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATc                                                                                               <  1:3986375/136‑1 (MQ=255)

                                              cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                                                                              >  1:191050/1‑140 (MQ=255)

                                                      ggTTTTAAACGCGTTTTCTCATCGATTTGTCAAAAAACGCTCGTCAAAATTTTATCCTTCCCCACAAAAAATTTTTAGTTGCCTGTTATCCATTTTTTATACAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt                                                                                      <  1:135780/139‑1 (MQ=255)

                                                         tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                                   >  1:4292264/1‑140 (MQ=255)

                                                         tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                                   >  2:755689/1‑140 (MQ=255)

                                                         ttcaCAAGCTTTTGAACATCGCTTTTTACAAAAACGCTCGTTAAACTTTTATCCTTTACCCCTAAAATGTGTTAGTTGCCTGATATCCGTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                                   <  1:4114980/137‑1 (MQ=255)

                                                          ttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCAGGATCCTCTATCGTCAcc                                                                                  >  2:5767886/1‑139 (MQ=255)

                                                            aCAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGcc                                                                                >  1:4360343/1‑140 (MQ=255)

                                                                gtgTTTTCTCATCTCAGTGTAAAAAAAATCTGGTGACATTTTTATCCTTAACCACTAAAATTTTTTAGTTGCCTGATATACTTTTTTTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                                                                            <  1:2355848/138‑1 (MQ=255)

                                                                 gtttttCTAATCGAATTGTCAAAAAACGCTCTTTAAACTTTTATCCTTTACAACTAAAAATTTTTAGTGGCCTTATATCCTTTTTTTATTAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                           <  1:294454/138‑1 (MQ=255)

                                                                 ggTTTTCTCTTCGCATTGTCCTAAAAAGCTCTTTAAACTTTTATCCTTTAGCACGAAAATGTGTTACTTGCCTGATATCCCTTTTGTTTTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                           <  2:5097251/139‑1 (MQ=255)

                                                                 ctttttCTAATCGATTTTTCATAAAAAGCTCTTTAAATTTTTTTCCTTTACCACTAAAAATTTTTTGTTGCTTGATATCCCTTTTTTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                           <  1:4625966/138‑1 (MQ=255)

                                                                 cttttaCTCATCGCTTTTTCATAAAAAGCTCGTTAAAATTTTATCTTTTAGCACCAAAAAGTTTTACTGGCCTTATATCCCTTTTTTTTTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                           <  1:1282538/138‑1 (MQ=255)

                                                                 cGTTTTCTCATCGAATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGAACTAAAAAGAGTTACTTGCCTTATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                           <  2:2536827/140‑1 (MQ=255)

                                                                   ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                                                                         >  1:3152376/1‑140 (MQ=255)

                                                                   ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                                                                         >  1:1799860/1‑140 (MQ=255)

                                                                    tttCTCATGGCTTTTTCAAAAAACCCTCTTGAAATTTTTATCCTTTACCACCAAAAAGTGTTAGTTGCCTGATATCCCTTTTTTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                                        <  1:4827987/140‑1 (MQ=255)

                                                                     ttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCt                                                                       >  1:1185492/1‑140 (MQ=255)

                                                                            cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc                                                                >  1:393595/1‑140 (MQ=255)

                                                                            cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc                                                                >  1:5106815/1‑140 (MQ=255)

                                                                                ttGTCATAAAAAGCTTGCGAAACTTTCATCCTTTAGCATAAGAAAGAATCACTTGCCTGATATCCCTTTTGAATTCATAAAAACGATCCGCCAGCCGTCCGGGTCCTCTATCGTCACGCCCCCATTGCTCCAGTACGGGt                                                            >  2:13911/1‑140 (MQ=255)

                                                                                  gTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtc                                                          <  2:228270/140‑1 (MQ=255)

                                                                                   tCCTAAAAAGCTGGTGAAACTTTTATCCTTTCCCACCAAAAAGAGTTAGTGGCCTGATATCCCTTTTGTATACAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct                                                         <  1:1749646/140‑1 (MQ=255)

                                                                                   tCCTAAAAAGCTCGTTAAACTTTTATCCTTCACCACCAAAAAGAGTTACTTGCCTTATATCCATTTTGTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct                                                         <  1:602749/140‑1 (MQ=255)

                                                                                   tCCTAAAAAGATCGTTAAATTTTTATCCTTTCGCACGAAAAAGAGTTAGTTGCCTTATATCCCTTTGGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct                                                         <  2:1284503/140‑1 (MQ=255)

                                                                                          ccgCTCGTCAAAATTTTAACCTTTCGACCGAAAAAGAGTTAGTGGCCTTATACCCCTTTTTTTTTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                  <  1:2780111/138‑1 (MQ=255)

                                                                                          cccctctTGAAACTTTTATCCTTTAGCACTAAAAACAGTTACGTGCCTGATATCCCGTTTGTATGCAGAACAACGACTCGCCAACCGTCCGGATCCTCCATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAtac                                                  <  2:5499240/137‑3 (MQ=255)

                                                                                          aaGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                  <  2:4443300/140‑1 (MQ=255)

                                                                                          aaGATCGTTAAACTTTTTTCCTTTACCACCAAAAATAGTTCGTTCCCTTATAACCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCGGATTc                                                  <  2:942616/140‑1 (MQ=255)

                                                                                             ctctTGAACCTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCATCATAGCCCTTTGGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               <  2:1022500/140‑1 (MQ=255)

                                                                                             cTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               <  2:4223704/140‑1 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCCGAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCGATGGCTGCAGTACGGGTTCTCGGAGTCaag                                               >  1:5455917/1‑139 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCCTGGCTCCAGTACGGGTTCTCAGATTCAAc                                               >  1:3410930/1‑140 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAtgctac                                               >  1:3330663/1‑135 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               >  2:656001/1‑140 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               <  2:1799860/140‑1 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               >  2:4636569/1‑140 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               >  2:1235423/1‑140 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               >  1:4484993/1‑140 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               >  2:2886630/1‑140 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCGATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                               >  1:2028682/1‑140 (MQ=255)

                                                                                             cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTGGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                                                 <  2:2070442/122‑1 (MQ=255)

                                                                                              tctttaaaaTTTTATCCTTTAGCACGAAAAAGATTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                                              <  2:4360343/135‑1 (MQ=255)

                                                                                              tCGTCAAAATTTTATCATTTACCACCAAAAATTGTTACTTGCCTGATATCCCTTTTTTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                                              <  1:2547113/140‑1 (MQ=255)

                                                                                               cGTGAAATTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACct                                             <  2:5317096/140‑1 (MQ=255)

                                                                                               cGTGAAAGTTTTATCCTTTAGCACGAAAAAGATTTACTTGCCTTATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACct                                             <  2:1216129/140‑1 (MQ=255)

                                                                                                    aatttttATCCTTTACCACGAAAAAGTGTTACTTGCCTGATAACCCTTTTTTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                        <  1:4678542/137‑1 (MQ=255)

                                                                                                    aaCTTTTATCCTTTAGCACGAAAAATAGTTACTTGCCTGATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                        <  2:284970/140‑1 (MQ=255)

                                                                                                    aaCTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                        <  2:366067/140‑1 (MQ=255)

                                                                                                    aaCTTTTACCCTTTACAACTAAAAATAGTTTCTGGCCTGATATCCCTTTTGTATAAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                        <  1:2199022/140‑1 (MQ=255)

                                                                                                     atttttATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGAt                                       <  2:3943342/138‑1 (MQ=255)

                                                                                                     aCTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGAt                                       <  2:1935046/140‑1 (MQ=255)

                                                                                                     aCTTTTAGCCTTTCGCACGAAAAAGAGTTACTTGCCTGATAGCCCTTTTCTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGAt                                       <  2:3227011/140‑1 (MQ=255)

                                                                                                     aCTTTTACCATTTCCCACCAAAAATAGTTACTGCCCTCATAGCCCTTTTGTATCAATAACCACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGAt                                       <  1:3638130/140‑1 (MQ=255)

                                                                                                           aTCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa                                 <  2:2173116/140‑1 (MQ=255)

                                                                                                            tCCTTTAGCACGAAAAAGTGTTTGTTGCCTGATTTCCCTTGTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCAt                                <  2:2764330/140‑1 (MQ=255)

                                                                                                                 tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                           >  1:4934215/1‑140 (MQ=255)

                                                                                                                  cgCACGAAAAAGCGGTCCTGGCCTGATACCCCTTTTTGATCCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                          <  2:1585579/139‑1 (MQ=255)

                                                                                                                  aGCACGAAATAGCTTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTCCGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                          <  2:3779996/140‑1 (MQ=255)

                                                                                                                  aGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                          <  2:3309128/140‑1 (MQ=255)

                                                                                                                  aGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                          <  2:2412794/140‑1 (MQ=255)

                                                                                                                  aGCACGAAAAAGAGTTACGTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCACCCGTCCGGATCCTCTCTCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                          <  2:5614657/140‑1 (MQ=255)

                                                                                                                        aaaaaGAGTTACTTGCCAGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCACCCTCTTGTTAGCCCCTGTGTGTTAGCtt                    >  2:3152240/1‑140 (MQ=255)

                                                                                                                                 tACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGGTTTAGCTTTGAAGTGAt           >  1:163046/1‑140 (MQ=255)

                                                                                                                                  aCTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATc          >  2:1989922/1‑140 (MQ=255)

                                                                                                                                   cTTGCCTGCTATCCCTTTTGTAGTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCg         <  2:5256376/140‑1 (MQ=255)

                                                                                                                                   cTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCg         >  2:4300688/1‑140 (MQ=255)

                                                                                                                                   cTTGCCTGACATCCCCTTTCTATGAATAACAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCg         <  1:3454780/140‑1 (MQ=255)

                                                                                                                                     tGCCTTATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCt       <  1:2572194/140‑1 (MQ=255)

                                                                                                                                     tGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCt       <  1:1430884/140‑1 (MQ=255)

                                                                                                                                     tGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCt       >  2:5712090/1‑140 (MQ=255)

                                                                                                                                     tGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCt       >  2:4099411/1‑140 (MQ=255)

                                                                                                                                     tGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCt       >  2:3852795/1‑140 (MQ=255)

AATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAG  >  NZ_CP034943/3996772‑3997049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`