BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	4,000,117	G→T	7.6%	L121F (TTG→TTT)	EO946_RS20555 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	4,000,117	0	G	T	7.6%	1307.0 / 25.5	489	L121F (TTG→TTT)	EO946_RS20555	MFS transporter
Reads supporting (aligned to +/- strand): ref base G (127/309); new base T (51/2); total (178/311)
Fisher's exact test for biased strand distribution p-value = 1.52e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 492 total aligned reads displayed.

CGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGAAGC  >  NZ_CP034943/3999979‑4000256

 ggAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGcc                                                                                                                                           <  2:4895073/140‑1 (MQ=255)

     aTGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCGTTATTTTTCGGAACGGCACGGAAAGGAGGCGGA‑AATGCGATAGGTAACGTTCTTTTTCCAAGc                                                                                                                                       >  1:3885161/1‑140 (MQ=255)

        aGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACACATCGGAGTAGGA‑ATTGCGATCGGAAACGTTATTTTGCCAAGCTTa                                                                                                                                    >  2:3893366/1‑140 (MQ=255)

         gCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTaa                                                                                                                                   <  2:5055901/140‑1 (MQ=255)

          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAt                                                                                                                                  <  1:1590082/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  1:2075280/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  1:22232/140‑1 (MQ=255)

            aaCCTTATGGATTGGTCTCCTGTTACTGTTTATCGGCCTATAGATTCGTTCTGCCGGCTATACTGCCGCTTTCTTTTTAGGAACGGCACTCATCGGAGTCGGT‑CTTGCGATCGGAAAAGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  1:4182575/140‑1 (MQ=255)

              ccTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCaaa                                                                                                                              <  2:2445526/140‑1 (MQ=255)

                  aTGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTTTTTTTAGGTACGGCACTCATCGGGGTGGGACATTG‑GATCGGCAACGGTGGTTTTCCAAGCTTAATAAAACaaa                                                                                                                          >  1:1643459/1‑138 (MQ=255)

                   tGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATaa                                                                                                                         <  1:4732325/140‑1 (MQ=255)

                        atgTCTCCTGATCCGGTGTTTCGGCTTTTTGATTCGTTCGGCCGACTATACTGGCGCTTTATTTGTCGGCACGGTCCTGATCTGAGTCGGA‑ATTGCGATCGGAATAGTTCTTTTGCCAATCTTATTCACCCATACTTAcc                                                                                                                    <  2:4574149/138‑1 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGTTTCGTTCTTCCGGCTATACTGCCGCTTTATTTTTAGGAAAGGCACTAATCGGTGTCGGA‑ATTGCGATCGGAAACGGTCTTTTGCCAAGGTTAATCAAAAAGAAATAacc                                                                                                                   >  2:2884914/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTTCCGCGTTATTTTTCGGAACGGCACTCATCGGAGTAGGC‑AGTGCGATCGGAAACGTTCGTTTGCCAAGCATACTCAAACATAAATAccc                                                                                                                   >  2:4677666/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGACTCGGA‑ATTGCGATCGGAAACGTTCTTTTTCCAACCTTAATCAAAAATAAATAccc                                                                                                                   >  2:5492914/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAACGGCACTCATCGGTGTCGGA‑AATTCGCTCCGACACGTTCATTTTCCCAGCGTAATCAAAAATaaaaaacc                                                                                                                   >  1:681803/1‑135 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAACGGCACTCAACGGAGTCGGA‑AATGCGATCGGAAACGTTGTTTTTCCAAGCTTAATCAAAAATaaaaaacc                                                                                                                   >  1:1375232/1‑135 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCGTTATTTTTGGGAAACGCCCAAATCGGAGTCGGC‑AATGCGATCGGGAACGGTCTTTTTGCAAGCTTAATAAAACAAAAATAacc                                                                                                                   >  1:1302909/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGGTATGCCGGCTATAATGCCGCGTTTTTTTTGGGAACGGGAAGCAACGGAGGCGGA‑AATTCGAAGGGCAACGGTGTTTTTGCAACCGTTATAAAAAATCAATAacc                                                                                                                   >  1:282046/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTGTGATTCGTTCTGCCGGCTATACTGCGGCGTTTTTTTTCGGAACGGCACTCATCGGAGTGGGA‑ATTGCGTTCGGAAACGTTCTTTTGCCAAGCCTAATCAAACATAAATAccc                                                                                                                   >  2:478443/1‑140 (MQ=255)

                           tctcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCCTCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTg                                                                                                                 >  2:1850978/1‑140 (MQ=255)

                           tctcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTTCCAAGCTTAATAAAACATAAATACCCTg                                                                                                                 >  2:1348562/1‑140 (MQ=255)

                           tctcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTg                                                                                                                 >  2:1994740/1‑140 (MQ=255)

                             tcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGATCGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGaa                                                                                                               <  1:187518/140‑1 (MQ=255)

                             tcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGaa                                                                                                               <  1:4469270/140‑1 (MQ=255)

                             tcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGaa                                                                                                               <  1:4938836/140‑1 (MQ=255)

                             tcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATCGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGaa                                                                                                               <  1:3234106/140‑1 (MQ=255)

                                  atacTGTTTATCGGCGTTTTGATTCGTTATGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTc                                                                                                          <  1:4263619/137‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAGACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  2:3022372/140‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  1:4638389/140‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  2:5603368/140‑1 (MQ=255)

                                        tttATCGGCGTTTTGATTCGTTCTGTCGGCTATCCTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAAGACCCTGAAAAGTCAGGGAt                                                                                                    <  2:901927/140‑1 (MQ=255)

                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                                                                    <  1:4349239/140‑1 (MQ=255)

                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                                                                    <  1:2502228/140‑1 (MQ=255)

                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                                                                    <  1:1055753/140‑1 (MQ=255)

                                        tttATCGGCGTGTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTTCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATCAATACCCTGAAAAGTAAGGGAt                                                                                                    >  2:5539119/1‑140 (MQ=255)

                                        ttgaTCGGCGTTTTGTTTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGTCACTCATCGGAGTCTGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCATACATAAATACCCTGAAAAGTCAGGGAt                                                                                                    <  2:1407844/137‑1 (MQ=255)

                                             cGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGa                                                                                               <  1:2565335/140‑1 (MQ=255)

                                              ggCTTTTTGATTCGTTCTGCCGGATATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGGTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  1:3960627/140‑1 (MQ=255)

                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  1:2684260/140‑1 (MQ=255)

                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  1:5184763/140‑1 (MQ=255)

                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  1:633153/140‑1 (MQ=255)

                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATATTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  1:1229799/140‑1 (MQ=255)

                                              ggCGTTTTGATTCGTTCTGCCGGCGATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  2:273725/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  2:4400236/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  2:499485/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  2:103338/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGCAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  2:4054756/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGATAGACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  2:2565763/140‑1 (MQ=255)

                                                 gTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAg                                                                                           <  2:4518182/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:111510/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:2645236/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:3515156/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:1429238/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:1514641/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:4797992/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:5064756/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCCGCTATACTGCCGCTTTATTTTTCGGCACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:3303345/140‑1 (MQ=255)

                                                  ttgtGAGTCGTTCTGCCGGCTATACTGCCGCTTGATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATCAATACCCTGAACAGTCAGGGATCATGATCAGc                                                                                          <  1:900834/137‑1 (MQ=255)

                                                            ttCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             >  2:3951491/1‑127 (MQ=255)

                                                            ttCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGa                                                                                <  2:68182/140‑1 (MQ=255)

                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           <  2:4305228/140‑1 (MQ=255)

                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           <  1:2111474/140‑1 (MQ=255)

                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           <  1:5582514/140‑1 (MQ=255)

                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCAGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           <  2:5747202/140‑1 (MQ=255)

                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGACTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCTAACATAAATCCCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           <  1:3727871/140‑1 (MQ=255)

                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          >  2:4182449/1‑113 (MQ=255)

                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTCTACGACATCAATGAATATATTc                                                               >  2:5233668/1‑140 (MQ=255)

                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAAAACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           >  2:3699494/1‑128 (MQ=255)

                                                                              cGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg                                                              >  2:2511025/1‑140 (MQ=255)

                                                                              cGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAAAATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATAttag                                                              >  2:5350662/1‑138 (MQ=255)

                                                                               gCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTg                                                                                                                 >  2:3418408/1‑88 (MQ=255)

                                                                                cTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCgct                                                            <  1:1761689/140‑1 (MQ=255)

                                                                                cTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGGTCTTTTGCCAAGCTTAATAAAACATAAATAACCGGAAAAGTCAGGGATCATGATCAGCCTGTATACCACATCAATGAATATATTCgct                                                            >  2:2356775/1‑140 (MQ=255)

                                                                                cTTTATTGTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCCGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCgct                                                            <  1:5458456/140‑1 (MQ=255)

                                                                                         gcGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTTCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCa                                                   <  1:2929412/139‑1 (MQ=255)

                                                                                          cGGACCGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCAt                                                  <  2:3483358/140‑1 (MQ=255)

                                                                                          cGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCAt                                                  <  1:4086999/140‑1 (MQ=255)

                                                                                          cGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCAt                                                  >  2:602645/1‑140 (MQ=255)

                                                                                           ggAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATc                                                 <  1:5535311/140‑1 (MQ=255)

                                                                                            gAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCa                                                <  2:2135819/140‑1 (MQ=255)

                                                                                              aCGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAACAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAgg                                              <  1:4547333/140‑1 (MQ=255)

                                                                                                 gCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGgtgt                                           <  1:53708/140‑1 (MQ=255)

                                                                                                      cATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCg                                      <  1:3613826/140‑1 (MQ=255)

                                                                                                       aTCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGt                                     >  1:1720590/1‑140 (MQ=255)

                                                                                                       aTCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGt                                     >  2:3081201/1‑140 (MQ=255)

                                                                                                          ggAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAat                                                                     <  2:1755621/105‑1 (MQ=255)

                                                                                                            aGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACctc                                >  2:3834227/1‑140 (MQ=255)

                                                                                                               cGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTgg                             <  1:3391918/140‑1 (MQ=255)

                                                                                                                 gA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCCGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcg                           >  1:3088690/1‑140 (MQ=255)

                                                                                                                 gA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcg                           <  2:3609539/140‑1 (MQ=255)

                                                                                                                 gA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcg                           >  2:1637549/1‑140 (MQ=255)

                                                                                                                 gA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcg                           <  2:1476387/140‑1 (MQ=255)

                                                                                                                 gA‑ATTGCGAGCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcg                           <  2:5422791/140‑1 (MQ=255)

                                                                                                                       gCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCgctgct                                                         <  1:5691846/105‑1 (MQ=255)

                                                                                                                         gATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTTAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACaaa                    >  2:1249289/1‑140 (MQ=255)

                                                                                                                             ggAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAACAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATggg                <  1:2341741/140‑1 (MQ=255)

                                                                                                                             ggAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATggg                <  2:5149563/140‑1 (MQ=255)

                                                                                                                               aaaCGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAg              <  1:1131742/140‑1 (MQ=255)

                                                                                                                                    ttCTTTTGCCACGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAACAGCCTGTATACGCCATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGa         <  1:803685/140‑1 (MQ=255)

CGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGA‑ATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGAAGC  >  NZ_CP034943/3999979‑4000256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`