Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 4,022,645 G→C 5.2% A623P (GCA→CCA)  EO946_RS20675 → thioredoxin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349434,022,6450GC 5.2% 1066.4 / 4.5 410A623P (GCA→CCA) EO946_RS20675thioredoxin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (158/217);  new base C (2/29);  total (161/249)
Fisher's exact test for biased strand distribution p-value = 3.12e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 414 total aligned reads displayed.

TGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTGTATATTTGTGAAAACTTCG  >  NZ_CP034943/4022506‑4022783
                                                                                                                                           |                                                                                                                                          
tgtgTTCAAGCCGGATATCGAAGCGTATCCGAGCGTCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTg                                                                                                                                            <  1:1409722/140‑1 (MQ=255)
tgtgTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTg                                                                                                                                            <  2:4812204/140‑1 (MQ=255)
tgtgTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTg                                                                                                                                            <  1:854289/140‑1 (MQ=255)
 gtgtTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGc                                                                                                                                           <  2:2371240/140‑1 (MQ=255)
  tgtTCAAGCCGGATATCGCAGCGTATCCGAGCGGCCATTCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGACTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACTATCCAGCCAGAAAACAAATCACCACTGCa                                                                                                                                          <  1:187495/140‑1 (MQ=255)
       aaGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCa                                                                                                                                     >  1:2099320/1‑140 (MQ=255)
       aaGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCa                                                                                                                                     >  1:3325418/1‑140 (MQ=255)
            ggATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAg                                                                                                                                >  2:2589384/1‑140 (MQ=255)
            ggATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAg                                                                                                                                >  1:3047656/1‑140 (MQ=255)
                atCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCtt                                                                                                                            <  2:5693337/140‑1 (MQ=255)
                 tCGAAGCTTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCttt                                                                                                                           <  2:4588468/140‑1 (MQ=255)
                  cGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTa                                                                                                                          <  2:4518309/140‑1 (MQ=255)
                       cGTATCCTCGCGGCCTGGCTTTCTTTCTGAAATGTGTGTTAAAGCTTTTGATGCCTAAAAAGTAAATCGTTATTTTTGGCAGCGCAGACGATCCTGCAAGAAAACAAATCACCACTGAAATTAAAAAACCCTTTAAGccc                                                                                                                     <  2:2904835/140‑1 (MQ=255)
                           tCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACg                                                                                                                 >  2:1050689/1‑140 (MQ=255)
                           tCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACg                                                                                                                 >  1:3548322/1‑140 (MQ=255)
                               aGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTc                                                                                                             >  2:3560109/1‑140 (MQ=255)
                                   gCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCAtt                                                                                                         >  1:2449942/1‑140 (MQ=255)
                                   gCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCAtt                                                                                                         >  2:408266/1‑140 (MQ=255)
                                    ccATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCAttt                                                                                                        >  1:1545735/1‑140 (MQ=255)
                                    ccATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCAttt                                                                                                        >  2:1117444/1‑140 (MQ=255)
                                         ctttctttCTTCAAAGCGTGTTAACGCATGTGATGCCTAAAAAGGAAATCGTTATTGTGGGCAGCGCAGACGATCCCGCCACAAAAAAAATCACCACGGCAATTCAAAACGCCTTTAAGCCCACCGACTCCATTTtggtg                                                                                                   <  2:608365/140‑1 (MQ=255)
                                         ctttctttATGCAAAGCGTGTTAAAGCATGTCATGCCTAAAAACGAAATCGTTTTTTTTGCCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAACCCCAACGACTCCATTTtggtg                                                                                                   <  2:1735261/140‑1 (MQ=255)
                                         cgttctttATCAAAAGCGTTTTAAAGATTGTGAGGCCAAAACACCAAATCGTTATTTTTGGCAGCGCACACCATCCCCCAAAAAAAAAAATCACCACTGCACTTCAAAACGCCTTTAACCCCCACGACTCCATTTtggtg                                                                                                   <  1:3782388/138‑1 (MQ=255)
                                              ttttTGAAAAGCTTTTAAAAGCTTGTGATGCCTAAAAAGTAAATCGTTATTTTTGGCAGCGCCCACTAACCCCCCAGAAAAAAAATAACCACTCCACTAAAAAAAGCCTTTAACCCCAACGACTCCATTTTGGTGGCaga                                                                                              <  1:3989190/140‑1 (MQ=255)
                                              tttATGCAAAGCGTGTTAAAGCATGTTATGCCTAAAAAGCAAATCGTTATTTTTGGCAGCGCAGACGATCCACCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                                                                              <  2:51000/140‑1 (MQ=255)
                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                                                                              >  1:5361988/1‑140 (MQ=255)
                                              tttATGAAAAGCTTTTTAACGCATGTGAGGCATAAAAAGCAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATAACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                                                                              <  2:5163604/140‑1 (MQ=255)
                                              tttATGAAAAGCGTGTTAAACCATGTGATGCCTAAACAGGAAATCGTTATTTTTGGCAGCGAAGACGAGCCAGCCACAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAACCCCAACGACTCCATTTTGGTGGCaga                                                                                              <  2:4847259/140‑1 (MQ=255)
                                                tatcaaaaGGGTTTTAACGCATGTTATGAATAAAACGGAAATCGTTTTTTTTGGCACCGCTGACGATCCAGCCACAAAACAAATCACCAGTGCATTTAAAAAACCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGc                                                                                            <  2:627477/135‑1 (MQ=255)
                                                  tGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCAt                                                                                          >  2:3732855/1‑140 (MQ=255)
                                                  tGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCAGTTTGGTGGCAGAGCAt                                                                                          >  1:2873604/1‑140 (MQ=255)
                                                  tGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACGTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCag                                                                                          >  2:3871921/1‑139 (MQ=255)
                                                   gCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATc                                                                                         >  1:1193255/1‑140 (MQ=255)
                                                    cAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATcc                                                                                        >  1:3441521/1‑140 (MQ=255)
                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                                                                     >  1:3162010/1‑140 (MQ=255)
                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                                                                     >  1:608606/1‑140 (MQ=255)
                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                                                                     >  2:581215/1‑140 (MQ=255)
                                                        gCGTGTAAAAGCATGTGATGCCTAAAAAGGAAATCTTTATTTTTGGCAGCGCAGACGACCCAGCCTGAAAACAAATCACCACTGCACTTCAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGAt                                                                                    <  2:198615/140‑1 (MQ=255)
                                                         ggtgtTAAACCATGTGTTGCCTAAAAAGGAACTCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACAACTGCACTTAAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATc                                                                                   <  1:804895/139‑1 (MQ=255)
                                                         cGTGTTACATCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCCACGACTCCATTTTGGTGGCAGAGCATCCTGATc                                                                                   <  2:2739444/140‑1 (MQ=255)
                                                            gtTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATc                                                                                   <  2:717563/137‑1 (MQ=255)
                                                            gtTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAAt                                                                                >  2:3925747/1‑140 (MQ=255)
                                                             ttAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCa                                                                                                                                          <  2:2503874/81‑1 (MQ=255)
                                                              tAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGc                                                                              <  1:2695814/140‑1 (MQ=255)
                                                               aaaGCTTGTTATGCCTACAAAGTAAATGGTTATTTTTGGCAGCGCAGACTATCCAGCCACAAAACAAATCACCACTGCACTCCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCa                                                                             <  1:4514907/140‑1 (MQ=255)
                                                                aaGCATGTGATGCCTAAAAAGTAAATCTTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                                                                            <  2:2837230/140‑1 (MQ=255)
                                                                aaGCATGTGATGCCTAAAAAGGAAATCTTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                                                                            <  2:5552895/140‑1 (MQ=255)
                                                                aaGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                                                                            <  2:5358337/140‑1 (MQ=255)
                                                                aaGCATGTGATGCATAAAACGTAAATCTTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                                                                            <  1:5443632/140‑1 (MQ=255)
                                                                aaGCATGTGATGCAAAAAACGCAAATCTTTATTTTTGGCAGCGCAGACGATCCACCCACAAAAAAAATCACCACTGCACTAAAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                                                                            <  1:3639646/140‑1 (MQ=255)
                                                                 aGCATTTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCAATTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaaa                                                                           <  2:5294201/140‑1 (MQ=255)
                                                                 aGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaaa                                                                           <  2:2795331/140‑1 (MQ=255)
                                                                  gcttgttATGCATAAAAAGTAAATGTTTTTTTTTGGCAGCGCAGACGATCCACCCAAAAAACAAACCCCCCCTGCACTTCAAAACCCCTTTACGCCCCACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAg                                                                          <  1:698281/137‑1 (MQ=255)
                                                                  gaaTGTTAGGCCTAAAATCAAATTCGTTATTTTTGGAAGCGCATCCCAGCCCGCCAGAAAAAAAATCACCACTTCACTTCAAAAACCTTTTAACCCAAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAg                                                                          <  1:1026442/138‑1 (MQ=255)
                                                                  gaaTGTGATGCCTAAAACGTAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAg                                                                          <  2:2990385/138‑1 (MQ=255)
                                                                  gCATCTGATGCCTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAg                                                                          <  2:3427643/140‑1 (MQ=255)
                                                                   cATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGATAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCCTTTTGGTGGCAGAGCATCCTGATCAATGCAAAGa                                                                         >  2:1815953/1‑140 (MQ=255)
                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCATACGATCCAGCCAGAAAACAAATCACCACTGCACTGCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCAGGATCAATGCAAAGACa                                                                       >  1:1620042/1‑140 (MQ=255)
                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTGCATTTTGGTGGCAGAGCATGCTGATCAATGCAAAGAcc                                                                       >  1:3729251/1‑139 (MQ=255)
                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                                                                            >  2:4185130/1‑135 (MQ=255)
                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                                                                            <  1:4185130/135‑1 (MQ=255)
                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAg                                                                          >  1:5296352/1‑137 (MQ=255)
                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACa                                                                       >  2:4153114/1‑140 (MQ=255)
                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACa                                                                       >  1:1450619/1‑140 (MQ=255)
                                                                      gtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAACAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGTCAt                                                                      >  2:5489486/1‑140 (MQ=255)
                                                                       tgtttaatAAAAAGTAATTCTTTATTTTTGGCAGCGCATACGATCCTGCAATAAAACAAATCACCACTGAAGTAAAAAAAGCCTTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATa                                                                     <  1:1851491/133‑1 (MQ=255)
                                                                       ggATGCCTAAAAAGCAAATCGTTATTTTGGGCAGCGCAGACGATCCAGCCACAAAAAAAATCACCACGCCAATTCAAAACGCCTTTCACCCCCACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATa                                                                     <  1:2301752/139‑1 (MQ=255)
                                                                         atgcaaaaaaaGGAACTGGTTATTTTTGGAAGCGCATACGATCCAGCCACAAAAAAAAACACCACTCAACTACAAAAACCCTTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGc                                                                   <  1:1098006/134‑1 (MQ=255)
                                                                          tGCCTAAAACGTAAATCGTTATTTTGGGCAGCGCTGAATATCCAGCCATAAAAAAAATCACCAGTCCCCTACAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCa                                                                  <  2:2423240/140‑1 (MQ=255)
                                                                          tGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCa                                                                  <  2:3812330/140‑1 (MQ=255)
                                                                          tGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGAACTTCAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCa                                                                  <  2:1440555/140‑1 (MQ=255)
                                                                          tGCCTAAAAAGCAAATGTTTATTTTTGGCAGCGCAGACTATCAAGCAATAAAAAAAATCACCACTGCAATTAAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCa                                                                  <  1:484328/140‑1 (MQ=255)
                                                                           gCCTACAACGCAACTCGTTTTTTTTGGCAGCGCAGACGATCCAGCCACAAAACAAATCACCACTGCACTTCAAAACGCCTTAAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAc                                                                 <  1:5020955/140‑1 (MQ=255)
                                                                            ccTAAAACGGAAATCGTTATTTTTGGCAGCGCAGACGAGCCAGCCAGAAAACAACCCACCACTGAACTACAAACAGCATTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAcc                                                                <  1:5139599/140‑1 (MQ=255)
                                                                            ccTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTtggtg                                                                                                   >  1:3005323/1‑105 (MQ=255)
                                                                             cTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCCCTGCACTTCAAAAAGCCTTTAAGCCCAACGGCTCCATTTGGGTGGCAGAGCATCCTGATCAATGCACAGACATAGCTccc                                                               >  2:5271987/1‑140 (MQ=255)
                                                                             cTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAccc                                                               >  2:3025571/1‑140 (MQ=255)
                                                                               aaaaaGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCtt                                                             <  2:1193255/140‑1 (MQ=255)
                                                                               aaaaaGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGACCAACGACTCCATATTGGTGGCAGAGCATCCTTATCAATGCAAAGACATAGCAACCtt                                                             >  1:5540115/1‑140 (MQ=255)
                                                                                    ggAAATCGTTATTTTTGGCAGCGCAGACGATCCACCCAGAAAACAAATCACCACTGCACTAAAAAAAGCCTTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcag                                                        <  1:4644976/140‑1 (MQ=255)
                                                                                      aaaTCTTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcagca                                                      <  1:1336592/140‑1 (MQ=255)
                                                                                       aaTCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGcagcag                                                     >  2:4423739/1‑140 (MQ=255)
                                                                                           gTTTTTTTTGGCAGCGCAGACGATCCAGCAATAAAAAAAATCACCACTGAACTTCAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTa                                                 <  1:2803846/140‑1 (MQ=255)
                                                                                           gTTATTTTTGGCAGCGCAGACGATCCAGCAAGAAAACAACTCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTa                                                 <  2:4709085/140‑1 (MQ=255)
                                                                                             tATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACGGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTAcc                                               <  2:2178725/140‑1 (MQ=255)
                                                                                                        ccgcAGACGACCCCCCCACAAAAAAAATCCCCACGGCACGTCAAAAAGCATTTAAGCCCAACGACTCCCTTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGAc                                    <  1:256465/139‑1 (MQ=255)
                                                                                                                 aTCCAGCCCGAAAACAAAACACCACTGCAATTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAaaca                           <  1:5762327/140‑1 (MQ=255)
                                                                                                                  tCCAGCCAGAAAAAAAATCACCACTCCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAaacaa                          <  2:3541743/140‑1 (MQ=255)
                                                                                                                  tCCAGCCAGAAAAAAAATCACAACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAaacaa                          <  2:3256859/140‑1 (MQ=255)
                                                                                                                  tCCAGCAATAAAACAAATCACCACTGCACTTAAAAAAGCCTTTACCCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAaacaa                          <  2:1474212/140‑1 (MQ=255)
                                                                                                                    ccgccagAAAACAAATCACCCCTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAAcc                        <  1:2189220/138‑1 (MQ=255)
                                                                                                                    cagcaacAAAAAAAATCACCCCTGCACTAAAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAAcc                        <  1:4328820/140‑1 (MQ=255)
                                                                                                                          gAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTGtat                  >  2:1297198/1‑140 (MQ=255)
                                                                                                                                aaaTCACCACTGCACTTAAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTGTATATTtgt            <  2:4960709/140‑1 (MQ=255)
                                                                                                                                          tGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCt                                                              <  2:5635956/80‑1 (MQ=255)
                                                                                                                                          tGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCt                                                              >  1:5635956/1‑80 (MQ=255)
                                                                                                                                          tGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTGTATATTTGTGAAAACTTCg  >  1:4934700/1‑140 (MQ=255)
                                                                                                                                          tGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTGTATATTTGTGAAAACTTCg  >  2:5737777/1‑140 (MQ=255)
                                                                                                                                          tGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTGTATATTTGTGAAAACTTCg  >  1:1283092/1‑140 (MQ=255)
                                                                                                                                          tGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGAAATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACACACGTGTATATTTGTGAAAACTTCg  >  1:4775423/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTGTATATTTGTGAAAACTTCG  >  NZ_CP034943/4022506‑4022783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: