Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 44,870 2 bp→TC 100% intergenic (‑51/‑449) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494344,8700CT99.4% 646.8 / ‑4.6 173intergenic (‑51/‑450)abrB/metGtransition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (58/114);  total (58/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*NZ_CP03494344,8710GC99.4% 605.9 / ‑3.9 173intergenic (‑52/‑449)abrB/metGtransition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (58/114);  total (58/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 173 total aligned reads displayed.

CCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAA  >  NZ_CP034943/44740‑45000
                                                                                                                                  ||                                                                                                                                 
ccAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCattatt                                                                                                                           <  1:2194988/140‑1 (MQ=255)
     agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                                      <  1:3847429/140‑1 (MQ=255)
     agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                                      <  1:5259606/140‑1 (MQ=255)
     agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                                      <  1:5463304/140‑1 (MQ=255)
      gTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACaa                                                                                                                     <  2:3476544/140‑1 (MQ=255)
        acgacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCCAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACaaaa                                                                                                                   >  2:183993/1‑140 (MQ=255)
         cgacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAAt                                                                                                                  >  2:2750162/1‑140 (MQ=255)
          gacgCAGTTCGATAGGAATAACTACACCTCCTAATTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACATTCTCCTCCCAAGAGATACTTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATc                                                                                                                 <  1:3542278/140‑1 (MQ=255)
          gacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATc                                                                                                                 >  2:2216173/1‑140 (MQ=255)
          gacgCAATTCGATAGGAATAAATACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATc                                                                                                                 <  2:3627442/140‑1 (MQ=255)
            cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  1:3878891/140‑1 (MQ=255)
            cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  2:551688/140‑1 (MQ=255)
            cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  2:1144833/140‑1 (MQ=255)
            cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  1:684913/140‑1 (MQ=255)
            cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  2:5185193/140‑1 (MQ=255)
             gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCtt                                                                                                              <  2:3834535/140‑1 (MQ=255)
             gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCtt                                                                                                              <  2:5205824/140‑1 (MQ=255)
             gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCtt                                                                                                              <  2:2040437/140‑1 (MQ=255)
             gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTAATTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCtt                                                                                                              <  2:2187130/140‑1 (MQ=255)
              cAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACACTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTa                                                                                                             <  2:3259921/140‑1 (MQ=255)
                    gATAGGAATAACTACATGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                                       <  2:1014123/140‑1 (MQ=255)
                    gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                                       <  1:4823416/140‑1 (MQ=255)
                    gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                                       <  2:2072615/140‑1 (MQ=255)
                    gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCGTACCTTCa                                                                                                       <  2:1443882/140‑1 (MQ=255)
                      tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     <  1:5593970/140‑1 (MQ=255)
                      tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     <  2:942223/140‑1 (MQ=255)
                       aGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAg                                                                                                    <  2:4333060/140‑1 (MQ=255)
                             aaCGACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAAc                                                                                              <  1:3049637/140‑1 (MQ=255)
                              aCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACa                                                                                             <  1:2816091/140‑1 (MQ=255)
                              aCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACa                                                                                             <  1:4670817/140‑1 (MQ=255)
                                tACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAg                                                                                           <  1:2353842/140‑1 (MQ=255)
                                tACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAg                                                                                           <  1:4729793/140‑1 (MQ=255)
                                  cacGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGt                                                                                         >  1:5758602/1‑140 (MQ=255)
                                   acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCGTACCTTCATAGCCTAACAAGGtt                                                                                        <  2:1152565/140‑1 (MQ=255)
                                    cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     <  2:4421172/126‑1 (MQ=255)
                                    cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     >  1:4421172/1‑126 (MQ=255)
                                    cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  1:5241864/1‑140 (MQ=255)
                                    cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  2:1815434/1‑140 (MQ=255)
                                    cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  1:625569/1‑140 (MQ=255)
                                    cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  1:2288782/1‑140 (MQ=255)
                                       ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTcaa                                                                                    >  2:4564136/1‑138 (MQ=255)
                                       ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                    >  1:3980290/1‑140 (MQ=255)
                                       ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                    >  2:1932415/1‑140 (MQ=255)
                                       ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTAACTTCATAGCATAACAAGGTTTCCa                                                                                    >  2:3040460/1‑140 (MQ=255)
                                         tAGCTCATCAACTTTAAGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaat                                                                                  <  2:5663168/140‑1 (MQ=255)
                                               atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGAGTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                            >  1:2729851/1‑140 (MQ=255)
                                                tcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTc                                                                           >  2:4617204/1‑140 (MQ=255)
                                                tcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTc                                                                           >  2:5377496/1‑140 (MQ=255)
                                                 caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:3156112/1‑140 (MQ=255)
                                                 caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:633935/1‑140 (MQ=255)
                                                 caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:3371941/1‑140 (MQ=255)
                                                 caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:4743030/1‑140 (MQ=255)
                                                 caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:3776213/1‑140 (MQ=255)
                                                         cGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtttt                                                                  >  1:2718456/1‑140 (MQ=255)
                                                         cGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtttt                                                                  >  1:5405049/1‑140 (MQ=255)
                                                           tACAATACCAGTAGATTTCCTCAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTAGATTTATCTTTCTCATTATTCGACCAAATCCTTACCGTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                <  1:1284016/140‑1 (MQ=255)
                                                           tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                <  2:2447662/140‑1 (MQ=255)
                                                           gaCAATACCAGTAGATTTCATAAACGTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                <  1:3800089/139‑1 (MQ=255)
                                                              aaTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtttg                                                             <  2:3871775/140‑1 (MQ=255)
                                                                   cAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTatt                                                        <  1:1469899/140‑1 (MQ=255)
                                                                   cAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTatt                                                        <  1:1733059/140‑1 (MQ=255)
                                                                     gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:1204894/140‑1 (MQ=255)
                                                                     gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:1155426/140‑1 (MQ=255)
                                                                     gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:3572626/140‑1 (MQ=255)
                                                                     gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:4580943/140‑1 (MQ=255)
                                                                     gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:581481/140‑1 (MQ=255)
                                                                     ctAGAATTCATAAACTTTCGCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAACGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:3795990/139‑1 (MQ=255)
                                                                      tAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgat                                                     >  1:2032941/1‑140 (MQ=255)
                                                                      tAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgat                                                     >  1:1474761/1‑140 (MQ=255)
                                                                          tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                 <  1:1485513/140‑1 (MQ=255)
                                                                          tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                 <  2:5578298/140‑1 (MQ=255)
                                                                          tttAATAAACTTTCTCCTCCCAAGAGATGACTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                 <  2:3742649/140‑1 (MQ=255)
                                                                                taaCTTTCTCCTCCCCAGATATGATTATTTGTATAAATGATATTTATCGTTCTCATTATTCGACACAATCCTGACCTTCTTAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTTTTTGCTATTGATTGTAAGAAtt                                           <  1:3531574/139‑1 (MQ=255)
                                                                                aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:1474761/140‑1 (MQ=255)
                                                                                aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:498342/140‑1 (MQ=255)
                                                                                aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  1:5545923/140‑1 (MQ=255)
                                                                                aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:833893/140‑1 (MQ=255)
                                                                                aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:2032941/140‑1 (MQ=255)
                                                                                  aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                         <  2:5758602/140‑1 (MQ=255)
                                                                                      tctcCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCt                                     >  1:3941089/1‑140 (MQ=255)
                                                                                        tcctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTGTCTCAGTATTCGACAAAATCCTTACCGTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTta                                   <  1:2974563/140‑1 (MQ=255)
                                                                                         cctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                                  <  2:4019966/140‑1 (MQ=255)
                                                                                          ctccCAAGAGATGATTATTTGTTTACATTATATTGATCTTTCTCATTATTCGACAACATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtata                                 <  2:3414105/140‑1 (MQ=255)
                                                                                          ctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtata                                 <  1:2750162/140‑1 (MQ=255)
                                                                                             ccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTc                              <  1:5151361/140‑1 (MQ=255)
                                                                                             ccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTc                              <  1:1943276/140‑1 (MQ=255)
                                                                                                 gagaTGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                          <  2:2350804/140‑1 (MQ=255)
                                                                                                 gagaTGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                          <  2:5610431/140‑1 (MQ=255)
                                                                                                 gagaTGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTACATAGCATAACAAGGTTACCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTcccta                          <  2:1443189/140‑5 (MQ=255)
                                                                                                   gatgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt                        <  2:167219/140‑1 (MQ=255)
                                                                                                   gatgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt                        <  2:2637186/140‑1 (MQ=255)
                                                                                                    atgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                       >  1:3066512/1‑140 (MQ=255)
                                                                                                    atgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                       >  1:2264918/1‑140 (MQ=255)
                                                                                                           tttGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTc                                      <  2:1327055/118‑1 (MQ=255)
                                                                                                               tttAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAact            <  2:1983543/140‑1 (MQ=255)
                                                                                                               tttAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAact            <  2:5784765/140‑1 (MQ=255)
                                                                                                               tttAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAact            <  2:2718456/140‑1 (MQ=255)
                                                                                                                  aaaTTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAGactact         <  1:3156112/140‑1 (MQ=255)
                                                                                                                  aaaTTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactact         <  1:3371941/140‑1 (MQ=255)
                                                                                                                         atTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGAATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGATCTTATAGTCAGTAATGTATCAGAAACTACTCaaaaaa  >  2:2982734/1‑140 (MQ=255)
                                                                                                                                  ||                                                                                                                                 
CCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAA  >  NZ_CP034943/44740‑45000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑