New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP034943	982440 =	156 (0.950)	4 (0.030)	4/248	8.3	2.7%	coding (851/1443 nt)	EO946_RS05080	peptidoglycan endopeptidase
	?	NZ_CP034943	= 982436	143 (0.950)					coding (855/1443 nt)	EO946_RS05080	peptidoglycan endopeptidase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGCGGAGATTCACTTTGGAAAATTGCAAACAACTATAACCTGACTGTACAGCAAATCCGAAACAGCAACAATCTAAAATCAGACATGCTGTACGTTGGACAAGTGCTGAAGCTGACAGGTAAAGCTTCTTCAGGCACTTCATCATCATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/982588‑982440 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcatcatcatcGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGATGTC  <  NZ_CP034943/982436‑982298                                                                                                                                                                                                                                                                                                  AGCGGAGATTCACTTTGGAAAATTGCAAACAACTATAACCTGACTGTACAGCAAATCCGAAACAGCAACAATCTAAAATCAGACATGCTGTACGTTGGACAAGTGCTGAAGCTGACAGGTAAAGCTTCTTCAGGCACTTC                                                                                                                                                      >  1:1261176/1‑140      AGATTCACTTTGGAAAATTGCAAACAACTATAACCTGACTGTACAGCAAATCCGAAACAGCAACAATCTAAAATCAGACATGCTGTACGTTGGACAAGTGCTGAAGCTGACAGGTAAAGCTTCTTCAGGCACTTCATCAT                                                                                                                                                 <  1:1654226/140‑1        ATTCACTTTGGAAAATTGCAAACAACTATAACCTGACTGTACAGCAAATCCGAAACAGCAACAATCTAAAATCAGACATGCTGTACGTTGGACAAGTGCTGAAGCTGACAGGTAAAGCTTCTTCAGGCACTTCATCATCA                                                                                                                                               <  2:2425048/140‑1                       TTGCAAACAACTATAACCTGACTGTACAGCAAATCCGAAACAGCAACAATCTAATATCAGACATGCTGTACGTTGGACAAGTGCTGAAGCTGACAGGTAAAGCTTCTTCAGGCACTTCATCATCATCGTCTTCTTCGTCA                                                                                                                                >  2:117449/1‑140                                          GACTGTACAGCAAATCCGAAACAGCAACAATCTAATATCAGACATGCTGTACGTTGGACAAGTGCTGAAGCTGACAGGTAAAGCTTCTTCAGGCACTTCATCATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGA                                                                                                             <  1:117449/140‑1                                            CTGTACAGCAAATCCGAAACAGCAACAATCTAAAATCAGACATGCTGTACGTTGGACAAGTGCTGAAGCTGACAGGTAAAGCTTCTTCAGGCACTTCATCATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACG                                                                                                           <  1:2423505/140‑1                                                                                                            GAAGCTGACAGGTAAAGCTTCTTCAGGCACTTCATCATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAG                                           <  2:2505826/140‑1                                                                                                                                            CATCATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACATGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAA           >  1:568798/1‑140                                                                                                                                             ATCATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAA          <  1:153947/140‑1                                                                                                                                             ATCATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAA          <  1:1015408/140‑1                                                                                                                                              TCATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAAC         >  2:1914820/1‑140                                                                                                                                                ATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGG       <  2:539078/140‑1                                                                                                                                                ATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGG       <  2:1672215/140‑1                                                                                                                                                ATCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGG       <  2:2063046/140‑1                                                                                                                                                 TCATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTAGGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGA      >  1:312720/1‑140                                                                                                                                                  CATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGTGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGAT     <  2:1597327/140‑1                                                                                                                                                  CATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGAT     <  2:69265/140‑1                                                                                                                                                  CATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGAT     <  2:1597683/140‑1                                                                                                                                                  CATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGAT     <  2:2354797/140‑1                                                                                                                                                  CATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACCACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGAT     <  2:97707/140‑1                                                                                                                                                   ATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGATG    >  2:1364636/1‑140                                                                                                                                                   ATCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGATG    >  2:969689/1‑140                                                                                                                                                    TCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGATGT   >  2:1413974/1‑140                                                                                                                                                    TCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGA                                  <  2:246210/109‑1                                                                                                                                                    TCGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGA                                  >  1:246210/1‑109                                                                                                                                                     CGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGATGTC  >  2:555962/1‑140                                                                                                                                                                                                                                                                                                  AGCGGAGATTCACTTTGGAAAATTGCAAACAACTATAACCTGACTGTACAGCAAATCCGAAACAGCAACAATCTAAAATCAGACATGCTGTACGTTGGACAAGTGCTGAAGCTGACAGGTAAAGCTTCTTCAGGCACTTCATCATCATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/982588‑982440 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcatcatcatcGTCTTCTTCGTCAAATGCCAGCTCAAGCACGACGACTACATACACGGTGAAAAGCGGCGATTCCTTATGGGTGATTGCTCAAAAGTTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAATAATTTAAAAACGGATGTC  <  NZ_CP034943/982436‑982298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.