Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943514,9680GT27.7% 153.0 / 22.7 84V128L (GTA→TTA) EO946_RS02645outer membrane lipoprotein carrier protein LolA
Reads supporting (aligned to +/- strand):  ref base G (48/12);  new base T (0/23);  total (48/36)
Fisher's exact test for biased strand distribution p-value = 4.55e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGAAAAAGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAGC  >  NZ_CP034943/514830‑515105
                                                                                                                                          |                                                                                                                                         
gctataaaGGACCAAAATCAAGTGTTTTTACGAAATGAAAACGGTGTTTTTGTTCTCACGCCGTATTTAAATAAGAGCTTCATGTTTCAAAGCGACTGGCCGAACAAAAGCAGCCAGGTCTACTTGTTTGAATCGCTCGt                                                                                                                                          <  1:2313721/135‑1 (MQ=255)
ccGAAAAAGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGt                                                                                                                                          <  1:1582340/140‑1 (MQ=255)
     aaaGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAgg                                                                                                                                     <  1:1572655/140‑1 (MQ=255)
                   aaGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGAc                                                                                                                       >  1:1907996/1‑140 (MQ=255)
                         ttttACCAATTAAAAACGTAGTTTTTGTTCTCACGCCTTCTTAAAATAAGAGATTCTGGTTAAAAAGCGACTGGCCCAACAACAGCACCCAGTTCTACTTTTTTGAATCGCTCGTAAAGTATGTAAAAAACGACTCGGAt                                                                                                                 <  1:290337/140‑1 (MQ=255)
                         ttttACCAAATGAAACCGGATTATTTTTTCTCACGCAGTATTTAAATAAGAGATTCAGTTTTAAAAGCGACTGGCCCAACAACACCAGCCAGGTCTACTTTTTTGAATCGCGCGTAAAGGATGTTCAAAACGACTCGGAt                                                                                                                 <  2:2366260/140‑1 (MQ=255)
                                      aaaCGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTg                                                                                                    >  1:825984/1‑140 (MQ=255)
                                      aaaCGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTGCTGGTTACAACGCGCCTGGCCGAACAACCGCCGCCTGTTATACTTGTTTGAATCGCTGTTAATGTATGTACAACACGACTCGGATGCAGTTTTCACTg                                                                                                    <  1:550290/140‑1 (MQ=255)
                                              tctttGTTCCCACGCCGTATTTAAATACCAGCTACAGTTTAAAAAGCGACTCCCCGAACAACAGCAGCAAGGACTACTTGTTTGAAACGCTCCTAAAGCATGTTAAAAACGACTCGGATGCAGTTTTCACTGCCaaagaa                                                                                            <  1:2305358/138‑1 (MQ=255)
                                              tATTTGTTCTCACGCCTTATTTAAATAAGAGCTTCCGTTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGTTCTACTTGTTTGAATCGCTCTTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCaaagaa                                                                                            <  2:1907996/140‑1 (MQ=255)
                                              tATTTGTTCCCCCGCCGTTTTTAAACAAGCGGTTCAGGTTTAAACGCGACTGCCCCAACAACAGCAGCCTGTTATAGTTTTTTCAATCGCTCTTAAAGCTTGTACAAAACGACTCGGATGCAGTTTTCACTGCCaaagaa                                                                                            <  1:672959/140‑1 (MQ=255)
                                               aTTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCaaagaaa                                                                                           >  2:2236307/1‑140 (MQ=255)
                                               aTTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCaaagaaa                                                                                           >  1:1024057/1‑140 (MQ=255)
                                                   gTTCTCAGGGCTTCTTTAAATACTCGTTTGTGGTTCAATCGCCACTGCCCGAAAAACACCCGCCTTGTCTAGTTTTTTGACTCGCTGTTAATGTATTTAAAACACGACTCGGATGCAGTTTTCACTGCCAaagaaaagaa                                                                                       <  1:64450/140‑1 (MQ=255)
                                                      ctcACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATa                                                                                    >  1:475511/1‑140 (MQ=255)
                                                      ctcACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATa                                                                                    >  1:957189/1‑140 (MQ=255)
                                                      ctcACGCCGTCTCTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATa                                                                                    >  1:580962/1‑140 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCTTTTTTAAACGCGACTGGCCCAACACCCGCAGCCTGTTCTAATTTTTTGAATCGCTCGTAACGGATGTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACg                                                                                  <  1:1396310/140‑1 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTCAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGACAAGAAATACg                                                                                  >  2:1011025/1‑140 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAAt                                                                                     <  2:786849/137‑1 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAAt                                                                                     >  1:786849/1‑137 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACg                                                                                  >  2:52063/1‑140 (MQ=255)
                                                          cGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGACAAGAAATACGTa                                                                                >  1:1411326/1‑140 (MQ=255)
                                                          cGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTa                                                                                >  1:643506/1‑140 (MQ=255)
                                                          cGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTa                                                                                >  1:2305055/1‑140 (MQ=255)
                                                          cGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTa                                                                                >  1:2304314/1‑140 (MQ=255)
                                                                 tttAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGaaa                                                                         >  2:1026155/1‑140 (MQ=255)
                                                                     aaTATGAGCTTCAGTTTTCAAAGCGACGGGCCTAAAAACAGCAGCCTGTTCTAGTTTTTTGAATCGCTCTTAAAGTATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTaa                                                                     <  1:1362817/140‑1 (MQ=255)
                                                                     aaTAAGAGCTTCTGTTTTAAACGCGAGTGGCCCACCAACCGCAGCCAGGTCTACTTGTTTGAATCGCTCTTAAAGTATGTTCAACACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTaa                                                                     <  1:231532/140‑1 (MQ=255)
                                                                       tAATAGATTCAGTTTTAAACGCGACTGGCCGAACAACAGCAGCCTGGTCTATTTTTTTGAATGGATCTTAAAGGATGTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTaaaa                                                                   <  2:2222924/140‑1 (MQ=255)
                                                                         agagCTTCTGTTTTAAAAGCGACTGGCCCAAAAACAGCAGCCTGGTCTACTTTTTTTAATCGCTCTTAACGGATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                 <  1:642330/140‑1 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                 >  1:1446732/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                 >  2:1203431/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTAGGTTGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGACACTAAAACg                                                                 >  2:1398290/1‑140 (MQ=255)
                                                                          gagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAATCGa                                                                >  1:594613/1‑140 (MQ=255)
                                                                           agCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGaa                                                               >  1:2483053/1‑140 (MQ=255)
                                                                           agCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGaa                                                               >  1:2048984/1‑140 (MQ=255)
                                                                           agCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGaa                                                               >  1:421346/1‑140 (MQ=255)
                                                                           agCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGaa                                                               >  1:1357112/1‑140 (MQ=255)
                                                                           agCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGaa                                                               >  1:2218550/1‑140 (MQ=255)
                                                                             cttatgttttAAACGCGACTGGCCCAAAAACAGCAGCCAGTTATAGTTGTTTTAATCGATCTTAAAGGATGTTCAACACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACt                                                             <  1:355220/133‑1 (MQ=255)
                                                                             cTTCTGGTTAAAACGCGACTGGCCTAACAACCGCAGCCATGTCTATTTTTTTGAATCGCTCTTAAAGCATTTAAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACt                                                             <  1:645652/140‑1 (MQ=255)
                                                                             cTTCAGTTTTCAAAGCGACTGCCCCAACAACAGCAGCCAGTTCTAATTGTTTGAATCGCTCTTAAAGGATGTACAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACt                                                             <  1:706589/140‑1 (MQ=255)
                                                                               tctgttttAAAAGCTAGTGGCCTAACAACAGCAGCCTGTTATACTTTTTTTAATCGATTTTAAAGTATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                           <  1:2107648/135‑1 (MQ=255)
                                                                               tccggTTTCAAAGCTACTGGCCTAACAAACGCCCCCTGTTATATTTGTTTGAATCGCGGGTAAAGCTTTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                           <  1:1445473/137‑1 (MQ=255)
                                                                               tCAGGTTTCAAAGCGACTGGCCGAACAACCGCACACATGTATAATTTTTTGAATCGCTATTAAAGTATGTAAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                           <  1:1378429/140‑1 (MQ=255)
                                                                               tCAGGTTTAAACGCGACTGGCCGAACAACAGCAGCCAGGTATACTTTTTTTAATCGCTCGTAACGTATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                           <  1:237805/140‑1 (MQ=255)
                                                                                  gttttCAACGCGACTGGCCTAACAACAGCAGCCAGGTTTAGTTTTTTTAATCGATCTTAAAGGATGTACAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAg                                                        <  1:1776322/138‑1 (MQ=255)
                                                                                    tttCAAAGCGACTGGCCTAAAAACAGCCCCCAGTTCTACTTTTTTGAATCGCTCTTAAAGTATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCa                                                      <  1:681913/140‑1 (MQ=255)
                                                                                            cgaggggCCCAACAACAGCAGCCTGTTATAGTGTTTTGAATCGATGGTAAAGCTTTTAAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACaaaa                                              <  1:1701093/135‑1 (MQ=255)
                                                                                            cGACTGGCAGAAAAACAGCAGCCTGGTTTATTTTTTTTAATGGGTCGTAATGTTTGTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACaaaa                                              <  1:2042120/140‑1 (MQ=255)
                                                                                               cTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAATACGACTCGGATGAAGTTTTCACTGACACAGAAACGAACTACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAAtgt                                           >  2:1781219/1‑139 (MQ=255)
                                                                                               cTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAAtgc                                           >  1:1679351/1‑140 (MQ=255)
                                                                                                   cccaacaccAGCAGCCAGGTATATTTTTTTGAATCGCTCTTAAAGGATTTACAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGcc                                       <  1:1129316/137‑1 (MQ=255)
                                                                                                      aacaacAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAAc                                    >  2:500930/1‑140 (MQ=255)
                                                                                                         aacaGCAGCAATTTATACTTTTTTTAATCGGTCTTAAAGGTTTTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCa                                 <  1:2351959/140‑1 (MQ=255)
                                                                                                         aacaGCAGCAAGGTCTACTTTTTTGAATCGCTCTTAAAGGATGTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCa                                 <  1:2208371/140‑1 (MQ=255)
                                                                                                         aacaGCACCCTGGTATACTTGTTTGAATCTCTCTTAATGTATGTAAAACACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCa                                 <  1:2208382/140‑1 (MQ=255)
                                                                                                              cagcCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAATCGAACTATCAGCATAACAAAATGCTGCCAACGCAAGaaa                            >  1:2267890/1‑140 (MQ=255)
                                                                                                                   aGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACg                       >  2:2124657/1‑140 (MQ=255)
                                                                                                                     gTCTACTTGTTTGAATCGCTCGTAAATTATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGtt                     >  2:947075/1‑140 (MQ=255)
                                                                                                                     gTCTACTTGTTTGAATCGATAGTAAAAGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGtt                     >  2:915682/1‑140 (MQ=255)
                                                                                                                          cTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACa                >  2:185771/1‑140 (MQ=255)
                                                                                                                            tGTTTGAATCGCTCTAAAAGTATGTTAAAAACGACTCGGATGAAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaa              <  2:1684101/140‑1 (MQ=255)
                                                                                                                             tttttAATGGATCTTAAAGGATTTACAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaa             <  1:1670400/139‑1 (MQ=255)
                                                                                                                             tttttAATCGCTATTAATGTATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaa             <  1:1399022/139‑1 (MQ=255)
                                                                                                                              ttttAATCGCTCTTAAAGTATGTTCAACACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAACCGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaaa            <  2:588347/140‑1 (MQ=255)
                                                                                                                               ttGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACCGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGcc                                       >  2:1871778/1‑112 (MQ=255)
                                                                                                                               ttGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACCGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGcc                                       <  1:1871778/112‑1 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCTAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg          >  1:2493446/1‑140 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCACAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg          >  1:45624/1‑140 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGGAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAACTCACGTGTAACaaaaatg          >  2:1673608/1‑138 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTATCAAAAAAg          >  1:2507349/1‑140 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg          >  1:805523/1‑140 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg          >  2:366048/1‑140 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg          >  1:2493573/1‑140 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg          >  1:941375/1‑140 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAAAACGTATTCGAAACTAAAACGAACAATCAGCATAACAAAAACATGCCAACGCAAGAAATCACGTATAaaaaaaaac          >  1:1983261/1‑139 (MQ=255)
                                                                                                                                tGAATCGCTCGTAAAGGATCTTCAAAACGACTCGGATGCAGATTACACTGACAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGGTTAACaaaaaac          >  2:1804685/1‑139 (MQ=255)
                                                                                                                                       cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCCAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACACCTAAGATATGAg   >  2:1972688/1‑140 (MQ=255)
                                                                                                                                       cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAg   >  2:429629/1‑140 (MQ=255)
                                                                                                                                       cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAg   >  2:1659633/1‑140 (MQ=255)
                                                                                                                                       cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATAACGTTTAACAAAAAAGATATGAg   >  1:2312075/1‑140 (MQ=255)
                                                                                                                                        tCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAGc  >  1:11970/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CCGAAAAAGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAGC  >  NZ_CP034943/514830‑515105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: