Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943595,2030CA25.2% 288.9 / 32.0 143T30K (ACG→AAG) EO946_RS03130MMPL family transporter
Reads supporting (aligned to +/- strand):  ref base C (29/78);  new base A (35/1);  total (64/79)
Fisher's exact test for biased strand distribution p-value = 1.31e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 143 total aligned reads displayed.

ATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTGAAGAA  >  NZ_CP034943/595068‑595341
                                                                                                                                       |                                                                                                                                           
atatTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTGTCCGAACTTAAAGGAg                                                                                                                                         >  1:1565893/1‑140 (MQ=255)
atatTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTATCCGAACGTAACGGAg                                                                                                                                         >  1:1956860/1‑140 (MQ=255)
atatTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTGTCCGAACTTAAAGGAg                                                                                                                                         >  1:752028/1‑140 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTATTTTTTTTTATACGAACTTAAAGGAgat                                                                                                                                       >  1:2114565/1‑138 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTATTTTTATAAGAAAATAACGCAGCt                                                                                                                                       >  2:1096776/1‑140 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTATCCGAACTTAAAGGAGCt                                                                                                                                       >  2:842511/1‑140 (MQ=255)
   ttCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTCTGCGAACGTTACGGAGcgg                                                                                                                                      >  1:1294415/1‑138 (MQ=255)
     cACCATTCTAAAAAGCAAATTTAAGGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGc                                                                                                                                    <  2:2237882/140‑1 (MQ=255)
     cACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTGTACGAACTTAACCGAGCTGGc                                                                                                                                    >  1:2351213/1‑140 (MQ=255)
     cACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGc                                                                                                                                    <  1:136382/140‑1 (MQ=255)
        cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTGGTTTTTTTTGTGCGAACATAAAGGAGCGGGCCaa                                                                                                                                 >  1:464159/1‑140 (MQ=255)
          ttCTAAAAAGCAAATTTCAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTTTCCGAAAATAAACCAGCTGGCCAAta                                                                                                                               >  1:694149/1‑139 (MQ=255)
          ttCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTTTACGAACTTAAACGAGCTGGcccaaa                                                                                                                               >  1:1445726/1‑136 (MQ=255)
           tCTAAAAAGCCCATTTAGAGGAGGAGATTCTGCAGAATGAGAGCAATCATCCCATTCAAGTGGGCAAGTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTGTGGGTGATCCGAACTTAACGGCGCTGGcgatgga                                                                                                                              >  1:223432/1‑134 (MQ=255)
           tCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATGCGAAATTAAAGGAGCTGGCCAATCa                                                                                                                              >  1:2144853/1‑140 (MQ=255)
           tCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCa                                                                                                                              >  1:1239647/1‑140 (MQ=255)
           tCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTACGAACTTAACGGAGCTGTCCAAACa                                                                                                                              >  1:2256626/1‑140 (MQ=255)
            cTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAAGTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGATTTTTTTTTCTACGAACATAAAGGCCCAGGCCAAACaa                                                                                                                             >  1:433966/1‑140 (MQ=255)
              aaaaaGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaaa                                                                                                                           <  2:2417214/140‑1 (MQ=255)
                 aaGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAggg                                                                                                                        <  1:1247657/140‑1 (MQ=255)
                  aGCATATTTAAAGGAGGAGCTTCTGCAGAATGAGACCAATCATCTAATTCAAGTGGGCAATTGCAGCGGTTATATTACCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                       <  2:2021690/140‑1 (MQ=255)
                  aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCACTTGCAGCGGTTAAATTAGCACTTACCGTCGTTTTTAGTTTTTTTTATCCGAAAATAAAAGAGCTATCCaaaaaaaactgg                                                                                                                       >  2:1351028/1‑136 (MQ=255)
                  aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATACGAACTTTAAGGAGCTGGCCAATCAAAAtggg                                                                                                                       >  1:1198433/1‑140 (MQ=255)
                   gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                      <  1:13599/140‑1 (MQ=255)
                   gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                      <  2:872592/140‑1 (MQ=255)
                   gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                      <  1:1197855/140‑1 (MQ=255)
                          tAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCa                                                                                                               <  2:340937/140‑1 (MQ=255)
                           aacggaggaGATTCTGCAGAATGAGAGCAATCAACAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAg                                                                                                              <  2:1379770/137‑1 (MQ=255)
                           aaaGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAg                                                                                                              <  2:2115126/140‑1 (MQ=255)
                             aggaggAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCt                                                                                                            <  2:637391/140‑1 (MQ=255)
                              ggaggaGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCtt                                                                                                           <  2:1239647/140‑1 (MQ=255)
                               gagtagATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTc                                                                                                          <  1:2457094/140‑1 (MQ=255)
                                aggagATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTcc                                                                                                         <  1:427766/140‑1 (MQ=255)
                                aggagATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGACAGGCCCAGCTTcc                                                                                                         <  2:370973/140‑1 (MQ=255)
                                      ttcttcCGATTGAGAGCAATCATAAAATTCAAGTGGGCAATGGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGa                                                                                                   <  2:1652211/140‑1 (MQ=255)
                                      ttCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGa                                                                                                   <  1:524538/140‑1 (MQ=255)
                                      ttCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGa                                                                                                   <  1:2028214/140‑1 (MQ=255)
                                        cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                                                 <  1:708540/140‑1 (MQ=255)
                                        cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                                                 <  2:1936866/140‑1 (MQ=255)
                                         tGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGc                                                                                                >  2:1971422/1‑140 (MQ=255)
                                         tGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCCATCAAAAGGGGCAGGCCCACCTTCCTGCAGATGc                                                                                                >  2:2374687/1‑140 (MQ=255)
                                              aaTGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCAGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGttt                                                                                           >  2:250854/1‑140 (MQ=255)
                                                  agagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGa                                                                                       <  1:1356962/140‑1 (MQ=255)
                                                   gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGaa                                                                                      <  2:1391075/140‑1 (MQ=255)
                                                        atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTGTTTTGTCCGAACATAAAGGAGCTGGCCAATCAAAAGGGGCACGCCCCCCGTCCAGCACATTCTGTTTGAGAAAAAGc                                                                                 >  1:815633/1‑140 (MQ=255)
                                                        atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTATCCGCACATAAAGGAGCTGGCCAAACAAAACGGGCACGCCCACCTTCCTGCAGATGCTGTTTCAGAAAGAGc                                                                                 >  1:2205061/1‑140 (MQ=255)
                                                         tcatcaAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTTGTTTGTTTTCTGCGAACTTAACGGAGCTGGGCCATCCAAAAGGGCAGGCCCCCCTTACTGCCGATGCTGTTTCAGAAAGAGCa                                                                                >  1:1525519/1‑140 (MQ=255)
                                                         tcatcaAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTATCCGAACTTAAACGAGCGGGCCCAAAAAAATGGGGCGGCCCAGCTTCCGGCAGATGCTGTTTGAGAAAGAGCa                                                                                >  1:1916424/1‑140 (MQ=255)
                                                          catcaAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTGTCCGAACATAAAGGAGCGGGCCACACAAAAAGGGCACGCCCCGCTTCCTGCAGATTCTGGTTCAGAAAGAACaa                                                                               >  1:2029321/1‑140 (MQ=255)
                                                              aaaTTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGc                                                                           <  1:2374687/140‑1 (MQ=255)
                                                                 ttCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTATTTTGTTTTCTCCGAACATAAAGGAGCGGGCCCAACAAAAGGGGCAGGCCCAGCTTCCTGCAGTTGCTGTTTCAGAAAGAGGaaagtgcct                                                                        >  1:2487068/1‑134 (MQ=255)
                                                                   cAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAt‑tt                                                                     <  2:464159/140‑1 (MQ=255)
                                                                          gCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTGTTTTGTCCGAACATAAAGGAGCTGGGCCAACAAAAGGGGCACGCCCCCCGTCCTGCAGATGCTGTTTGAGACAGAGCAAATGCCAG‑TTTAAAACa                                                              >  1:961319/1‑140 (MQ=255)
                                                                           cAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTTGTTTGTTTTGTCCGGACTTAAAGGAGCGGGGCCAACCAAAGGGGCACGCCCCGCGTCCTGCCGATGCTGTTTGAGAAAGAGCAAATGCCAT‑TTTAAAACaa                                                             >  1:352015/1‑140 (MQ=255)
                                                                           cAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTTTTTTGTGCGAACTTAAAGGAGCTGGCCCATAAAAAGGGGGAGGCCCCCCTTCCAGCCGATGCTGTTTCAGGAAGATCAAAAGCCATGTTTaaaaaa                                                              >  1:228722/1‑138 (MQ=255)
                                                                           cAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTATGCGAAATTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCCGCTGGCTGCAGATTCTGTTTAAGGAACAGCAAAAGCCAA‑TTTAaaaaaa                                                             >  1:1495402/1‑140 (MQ=255)
                                                                            cagtccAGCGGTGAGATTAGCACGGACCCTCGGTGTGAGTTTGTTTACCCCGAACATAACGGAGCTGGACAAACAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAg                                                            <  2:1332623/135‑1 (MQ=255)
                                                                            aaTTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAg                                                            <  2:72812/140‑1 (MQ=255)
                                                                              ttGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTGTTTTGTGCCAACTTAACCGAGCGTGGCAAACAAAAGGGGCAGGCCCCGCGTCCAGCCGATGCTGTTTGAGAAAGAGCAAAAGACAT‑TTTAAAAAAAGCg                                                          >  1:882297/1‑140 (MQ=255)
                                                                               tGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCgg                                                         <  1:242239/140‑1 (MQ=255)
                                                                                gCAGCGGTTATATTAGCACTTACCGTCGTGTTTAGTTTTTTTTGTAAGAAAATAAAGGAGCGGGGCCAAAAAAAGTGGCAGGCCCACCTTGCGTCAGATGGATTTTAAGAAAGAGAAAAATCCAT‑TTTAaaaaaaacagg                                                        >  1:945947/1‑135 (MQ=255)
                                                                                    cGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGagaa                                                    <  2:599034/140‑1 (MQ=255)
                                                                                    cGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGagaa                                                    <  1:1297087/140‑1 (MQ=255)
                                                                                    cGGTTATATGAGCACTTACGGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGGTGTTTCAGAAAGAGCTAATGCCAT‑TTTAAAACAAGCGGGagaa                                                    <  1:1737889/140‑1 (MQ=255)
                                                                                             tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACaataat                                           <  2:752028/140‑1 (MQ=255)
                                                                                             tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACaataat                                           <  1:1008121/140‑1 (MQ=255)
                                                                                                cACTTACCGTCGTTTTGAGTTTTGTTTGTCCGAACTTAACGGAGCTGGCCCATAAAAAGGGGCAGGCCCAGCTTCCGGCAGATGCTGTTTGACAAAGAGCAAAATCCAA‑TTTAAAAAAAGCCGGAGAAGAAAAAAATTCg                                        >  2:189741/1‑140 (MQ=255)
                                                                                                cACTTACCGTCGTTTTGAGTTTGTTTTATCCGAACTTAAAGGAGCTGGCCAAACAAAAGGGGCAGGCCCAGCTTCCTGCACATGCTGTTTCAGAAAGAGCACATGCCAA‑TTTAAAAAAACCGGGAGAAGACaaaaaatag                                        >  2:2256586/1‑136 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:2140096/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:879291/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:1919411/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:1445726/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:1236883/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGAGGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:1189221/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTGTCTCCGAACGTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAACACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:2086987/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGACTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  1:940462/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTGTTTTGTTTTTTTTGTGCGAACTTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCCGCTTCCTGCAGATTCTGTTTCAGAAAGAGCCAATGCCAT‑TTTAAAAAAAACGGGGGAAAACAAAAAATGGAt                                      >  1:451578/1‑140 (MQ=255)
                                                                                                     aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                       >  2:2191401/1‑57 (MQ=255)
                                                                                                     aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCAAACAAAAGGGGGAGGCCCAGCTTCCTGCAGATGCAGTTTCAGAAAGAGCCAATGCCAT‑TTTAAAAAAAGCGGGAGAAGACAATAATTAGATCAg                                   >  2:1744216/1‑140 (MQ=255)
                                                                                                     aCCGTCGTGTTGTGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCAAACAAAAGGGGCAGGCCCAGCTTGCTGCAGATGCTGTTTGAGAAAGAGCAAATGCCAT‑TTTAAAAAAAACGGGAGAAGACAAAAAATAGATCAg                                   >  2:129831/1‑140 (MQ=255)
                                                                                                      ccGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGt                                  <  2:393097/140‑1 (MQ=255)
                                                                                                      ccGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGt                                  <  1:1096776/140‑1 (MQ=255)
                                                                                                      ccGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGt                                  <  2:1565893/140‑1 (MQ=255)
                                                                                                        gtcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTAt                                <  2:815633/140‑1 (MQ=255)
                                                                                                         tcgtTTTGAGTTTGTTTTCTCCGAACTTACCGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGACCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc                               <  2:2487068/140‑1 (MQ=255)
                                                                                                         tcgtTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGACAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGACAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc                               <  2:1989095/140‑1 (MQ=255)
                                                                                                          cgtTTTGAGTTTGTTTTCTCCCAAATTAACAGAGCTGGCCAATAAAAATGGGCAGGCCCAGCTTCCGGCAGATGCTGTTTCAGAAAGATCAAATGCCAA‑TTTAAAAAAACCGGGAGAATACAAAAAATCGATCACTATct                              >  2:406938/1‑139 (MQ=255)
                                                                                                           gtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCCGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGGCAG‑TTTAAAAAAAGCGGGAGAAGACCAAAATTCGATCACTATCGt                             >  2:1841028/1‑140 (MQ=255)
                                                                                                             tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  2:1793133/140‑1 (MQ=255)
                                                                                                             tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  2:753430/140‑1 (MQ=255)
                                                                                                             tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  1:274212/140‑1 (MQ=255)
                                                                                                             tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  1:259851/140‑1 (MQ=255)
                                                                                                              ttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCtt                          <  2:352015/140‑1 (MQ=255)
                                                                                                                 aGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTAc                       >  2:470256/1‑140 (MQ=255)
                                                                                                                  gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa                      <  2:144222/140‑1 (MQ=255)
                                                                                                                           ctcCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCAc             >  1:604678/1‑140 (MQ=255)
                                                                                                                                 aCTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTg       <  1:1772313/140‑1 (MQ=255)
                                                                                                                                 aCTTAACGGAGCTGGCCAATCAAAAGGGGCACGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTg       <  1:1090049/140‑1 (MQ=255)
                                                                                                                                   ttAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaa     <  1:2008242/140‑1 (MQ=255)
                                                                                                                                     aaCGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaaga   <  1:1276601/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
ATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCAT‑TTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTGAAGAA  >  NZ_CP034943/595068‑595341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑