Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,730,075	0	G	C	29.6%	148.3 / 14.4	86	E166Q (GAA→CAA)	rodZ	cell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (33/24);  new base C (0/24);  total (33/53)
Fisher's exact test for biased strand distribution p-value = 1.67e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTT  >  NZ_CP034943/1729938‑1730213                                                                                                                                          |                                                                                                                                           gCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaagaa                                                                                                                                          >  1:214407/1‑140 (MQ=255)  cGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaagaaa                                                                                                                                         <  1:939271/140‑1 (MQ=255)  cGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaagaaa                                                                                                                                         >  2:1780499/1‑140 (MQ=255)    aTGTTAAAAATGCAGAATCTGAAAAATCAATTAAGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGaaagaaaaa                                                                                                                                       <  2:1294870/140‑1 (MQ=255)    aTGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGaaagaaaaa                                                                                                                                       <  2:1739415/140‑1 (MQ=255)            aaTGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAGGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaa                                                                                                                               >  1:561962/1‑140 (MQ=255)              tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAATAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGACGATaaaa                                                                                                                             >  2:1441868/1‑140 (MQ=255)              tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                             <  1:2221504/140‑1 (MQ=255)              tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                             >  1:2509112/1‑140 (MQ=255)              tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAAAAAAAGCAACAGCTCTGAAAAATAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                             >  2:1219725/1‑140 (MQ=255)                 ctcATCTGAAAAATCAATTACGCAAAGTCAAAGAAAGTAATAAATCCCAACAGATACAAAGCTAAAAGAGAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAACAAGAAGATaaaaacg                                                                                                                          <  2:956382/138‑3 (MQ=255)                 cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaaaat                                                                                                                          >  2:628879/1‑139 (MQ=255)                  gCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaaga                                                                                                                         >  1:822958/1‑140 (MQ=255)                   cATCTGAAAAATCAATTACGCAAATTTAAAGCAATTACTAAATCCCAAAAGATTCAACTCTAAAAGATAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                        <  2:933088/140‑1 (MQ=255)                   cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAACAAGAAGATAAAAaagaa                                                                                                                        >  1:2450195/1‑140 (MQ=255)                      cTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAgaagaa                                                                                                                     >  2:1660031/1‑140 (MQ=255)                         aaaaaTCAATTACTCAAATTTAAAGAAAGTACTAAATCCCAAATTATTCACCTATAAAAGATATTAAAAGAAACAGTTTTGAAAAAGTGGCAGAAACTAAAAAAGAAACGAAAGAAAAAGAATATAAAAAAGAAGAAAGc                                                                                                                  <  2:2149186/140‑1 (MQ=255)                         aaaaaTAAATTAATAAAAGTTAAAGAAATTAATAAATCAAAAAATATAAAACGCTAAAAGATAATCAAAGAAACAGCTATGAAAAAGAGCCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGc                                                                                                                  <  2:2309378/140‑1 (MQ=255)                           aaaTCATTTACGCAAAGTGAAAGCAAGTACTAAATCCCAAATTATTCAACGATAAAAGAGAATAAAAGAAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGa                                                                                                                <  2:2245274/140‑1 (MQ=255)                                 aTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGa                                                                                                          >  2:1752416/1‑140 (MQ=255)                                 aTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGa                                                                                                          >  2:568610/1‑140 (MQ=255)                                 aTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGa                                                                                                          >  2:568641/1‑140 (MQ=255)                                        tcaGTGAACTCACGTACGAAATCTCAAAACATTAAACGCTAAAAGATACTCAATGAAACACCCCTGAAAAAGAGGCATACACTAAAAAAGAAACGAAACAAACCCAAGATAAAAAAGAACAAAGCGACAGTGATAAACTg                                                                                                   <  2:2083565/138‑1 (MQ=255)                                        aaaGTGAAAGCAAGTACTAAATCCCAAAAGATTCAACGATAAAAGATAATCAAAGAAACACCTCTTAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAACAACAAAGCGACAGTGATAAACTg                                                                                                   <  2:784241/140‑1 (MQ=255)                                            ttaaccaaaGTACGAAACCCAAAAACTTTCAACGATAAAAGATAATCAACGCAATAGATCTCAAAAATAGGCAGACAATAAAAAACAAACCAAAAAAAAAAAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGgaga                                                                                               <  2:1385216/133‑1 (MQ=255)                                               aaGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTa                                                                                            >  2:2503399/1‑140 (MQ=255)                                               aaGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTa                                                                                            >  2:93148/1‑140 (MQ=255)                                                 gCAAGTACTAAATCCCAAAAGTTTCAACCATAAAAGATAATAAAAGCAACAGCTCTTAAAAAGTGGCAGACACTAAAAAAGAAACTAACGAAAACCAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTaaa                                                                                          <  2:936005/140‑1 (MQ=255)                                                    aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCACCAGCTCTGAAAAAGAGGCAGACCCGAAAAAAGAAACGAAAGAAATAGACGAGCCAAGAGAAGAAAGCGACAGTGCTAAACTGGAGATTCAAGCa                                                                                       >  1:2054366/1‑140 (MQ=255)                                                    aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                       >  1:237758/1‑140 (MQ=255)                                                     taaccaaaTCCAAAAACATACACCCAAAAAACACAACCAAACCAACAGCTCTCAAAAACAGCCACACACAAAAAAACAAACCAAACAAAAACAACAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                                      <  1:293116/135‑1 (MQ=255)                                                     caccGAAATCCAAAAACATACAACGCAAAAAGAGAAACAACCAACCAGATCTGAAAAAGAGGCAGACAAAAAAAAACAACCCAAACAAAAACAAGAAAAAACACAACAACGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                                      <  1:1214180/137‑1 (MQ=255)                                                      tACTAAATCCAAAAATATTCAACGCTAAAAGACAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAACGAAAAAGATGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                     <  2:1727920/140‑1 (MQ=255)                                                      tACTAAATCCAAAAATATTCAACGCTAAAAGACAATCAAAGAAACAGATCTGAAAAAGAGCCATACACTAAAAAAGAAACGAAAGAAAATGAAGAAAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                     <  2:1583470/140‑1 (MQ=255)                                                      tACTAAATCCAAAAAGATTCAACGAAAAAAGATAATCAAAGAAACAGCACTTAAAAAGAGGAACACAATAAAAAAAAAACGAAACAAAAAGAATACAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                     <  1:1168125/140‑1 (MQ=255)                                                        ctaaaTCCAAAAAGATTCACCGATAAAAGATACCCAAACCAACAGCTCTAAAAAAGAGCCACACAATAAAAAAGAAACCAAACAAAAACAAGAAAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                   <  1:93148/138‑1 (MQ=255)                                                        ctaaaTCAAAAAATATTAAACGATAAAAGATAATAAAACCAACAGCAATGAAAAATTGGAACAAAATAAAAAACAAACGAAACAAAAAGAAGATAAAAAATAACACAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                   <  1:1059757/138‑1 (MQ=255)                                                         gAAATCCCAAAAGATTCAACGCTAAAGGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                                  <  2:561962/140‑1 (MQ=255)                                                           aacccaaaaaTATTAAACCATAAAACACAAAAAAACCAACCCCTCTTAAAAAGAGCCACAAACTAAAAAACAAACCAAACAAAAAGAACATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCa                                                                                <  1:1752416/134‑1 (MQ=255)                                                              ccaaaacGATTAAACGCTAAAATAGAATCAAAGCAACTGCTCTGAAAAAGTGCCAGACACTAAAAAAGAAACGAACCAAAACGAAGATAAAAACCAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTg                                                                             <  2:980896/137‑1 (MQ=255)                                                                cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCCGACACTAAAAAAGAAGCGCACGAAAATGAAGATCAAAAAGAAGAAAGCGACAGGGATCGCGTGGAGATTCAGGCACCTGGCACGGag                                                                           >  2:2208552/1‑139 (MQ=255)                                                                cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATCAACTGGAGATTAAAGCCAGTGGCACTGac                                                                           >  2:1426472/1‑139 (MQ=255)                                                                cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACGAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGac                                                                           >  1:1224265/1‑139 (MQ=255)                                                                  aaaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAgg                                                                         >  1:425836/1‑140 (MQ=255)                                                                   caGATCCAACGCTAAAACACAAAAAAAGCACCGGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                        <  2:1253614/139‑1 (MQ=255)                                                                   aacaTTCAACGCTAAAACACACTCAAACAAACAGCTCTGAAAAATAGGCACACACTAAAAAACAAACTAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                        <  2:2450195/137‑1 (MQ=255)                                                                   aaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGACAAAGAGGCAGACACTAACAAAGACACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAAGTGGAGATTAAAGCAACTGGCACTGAAggg                                                                        >  1:2079125/1‑140 (MQ=255)                                                                   aaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGAAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                        <  2:2509112/140‑1 (MQ=255)                                                                        tCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAACGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                   >  2:2390107/1‑140 (MQ=255)                                                                           cccaaaaaaCACAATAAAAGAAACACCTCTCAAAAAGAGCCACACACTAAAAAACAAACCAACCAAAAACAACAAAAAAAACAACACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  1:93941/135‑1 (MQ=255)                                                                           actcTAAAAGAGAATCAAAGCAACAGATCTTAAAAAGAGGCATACACTAAAAAATAAACGAAAGAAAAAGAACATAAAACACAATAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:757630/137‑1 (MQ=255)                                                                           actcTAAAAGACAATCAAAGCAACAGCTCTGAAAAAGAGGCATACACTAAAAAATAAACCAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:1538074/137‑1 (MQ=255)                                                                           actcTAAAAAACAAACAAAGAAACATCTCTTAAAAATAGTAATACACTAAAAAATAAACGAAATAAAAATAAGATAAAAAATAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:2212661/137‑1 (MQ=255)                                                                           aagCTAAAATACAATCAAATCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAATAAAAAGAAGATAAAAAATAACAAAGCGACACTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:2212674/138‑1 (MQ=255)                                                                           aCGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                >  1:2206340/1‑140 (MQ=255)                                                                           aCGCTAAAAGACAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACTAAACAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:2212380/140‑1 (MQ=255)                                                                           aCGCAAAAACAGAACAAAACCAACAGCTCTCAAAAACCGCCACAAAATAAAAAATAAACAAAACAAAAAGAACTTAAAAAAGACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  1:466130/140‑1 (MQ=255)                                                                           aCGATAAAATATAATAAAACCAACAGCTCTTAAAAAGAGGCAGACACTAAAAAACAAACGAAACAAAAAGAAGATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:304008/140‑1 (MQ=255)                                                                           aCGATAAAATACAATAAAAGCAACAGATCTTAAAAAGAGGAAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:1074390/140‑1 (MQ=255)                                                                           aCGATAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGAACACACTAAAAAACAAACCAAAGAAAAAGAAGAAAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:311456/140‑1 (MQ=255)                                                                            ataaaaaaTATAATAAAAGCAACTGCTCTTAAAAATAGCCACAAAAAAAAAAACAAAAGAAACAAAAACATTATAAAAAACAACAAACCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACg                                                               <  1:1620335/136‑1 (MQ=255)                                                                                   acagaATCAAAGAAACAGCTCTTAAAAACAGGCAGAAAATAAAAAACAAACCAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                        <  2:1679899/138‑1 (MQ=255)                                                                                     tctttCAAAGCACCAGCTATCATAACGAGGCAGAAAGTAAAAAAGAAACCAAAGTAATAGAAGATAAAAAAGAAGAAAGCGAGAGTGATAAACTGGAGATTAAAGCACCTGGCACTGAAGGGTCATTAACGACTTATgta                                                      <  2:1485824/136‑3 (MQ=255)                                                                                     atactCAAAGCAACAGCTCTTAAAAAGAGCCAGACAATAAAAAAGAAACCAAACAAAAACAACAAAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                      <  1:1842450/136‑1 (MQ=255)                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                    >  2:2502130/1‑140 (MQ=255)                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                    >  2:1992812/1‑140 (MQ=255)                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                    >  2:258762/1‑140 (MQ=255)                                                                                       aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGAAACTGGCACTGAAGCGTCATTAACGACTTATGAAgt                                                    >  2:2295424/1‑140 (MQ=255)                                                                                         tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                  >  2:2347483/1‑140 (MQ=255)                                                                                                 aCAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCt                                          >  2:1210241/1‑140 (MQ=255)                                                                                                 aCAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCt                                          >  2:1326502/1‑140 (MQ=255)                                                                                                     ctctTAAAAAGAGGCACACAATAAAAAACAAACCAAACAAAAACAACAAAAAAAACAACACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgaga                                      <  1:568610/140‑1 (MQ=255)                                                                                                     caatGAAAACGAGGCAGACACTAAAAAACAACCCAAAGAAAAACAACACAAAAAACACCCAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgaga                                      <  1:568641/137‑1 (MQ=255)                                                                                                       ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                >  1:1699450/1‑112 (MQ=255)                                                                                                       ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                <  2:1699450/112‑1 (MQ=255)                                                                                                               gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                            >  2:306542/1‑140 (MQ=255)                                                                                                               gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                            <  2:425836/140‑1 (MQ=255)                                                                                                               gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                            >  2:1631390/1‑140 (MQ=255)                                                                                                               cagccacacacTAAAAAACAACCCACCCAAAAAGACCATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                            <  1:1426472/133‑1 (MQ=255)                                                                                                                       cacTAAAAAAGAACCGAAATAAAAAGACGATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                    <  1:2490622/140‑1 (MQ=255)                                                                                                                       cacTAAAAAAGAAACTAAAGAAAAAGAACCTAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                    <  1:2490625/140‑1 (MQ=255)                                                                                                                       aacTAAAAAACAAACCAAACAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                    <  1:844212/139‑1 (MQ=255)                                                                                                                       aaatAAAAATTAAACTAAACAAAAAGAATATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                    <  2:214407/137‑1 (MQ=255)                                                                                                                                 taaaCTAATGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa          <  2:1234849/139‑1 (MQ=255)                                                                                                                                 taaaCCAAAGAAAAACAATATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa          <  2:701741/139‑1 (MQ=255)                                                                                                                                 gAAACGAAAGACATAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGATGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCTTCAGa          <  2:1152441/140‑1 (MQ=255)                                                                                                                                 gAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa          <  2:237758/140‑1 (MQ=255)                                                                                                                                 gAAACCAAATAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa          <  2:1152538/140‑1 (MQ=255)                                                                                                                                 caaaCGACACAAACAGACCATAAAAACTAACAACGCGACCTTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAACTTCGAGCTCGAACTGAAGGCATCAGa          <  1:1624497/139‑1 (MQ=255)                                                                                                                                 caaaCCAAACAAAAATAAGAAAAAAAATAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa          <  2:1637695/139‑1 (MQ=255)                                                                                                                                    aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg       >  1:1849054/1‑140 (MQ=255)                                                                                                                                         agaaaaagaaGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCtt  >  1:1377017/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                           GCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTT  >  NZ_CP034943/1729938‑1730213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑