Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,293,3480GT30.2% 198.7 / 23.5 120V141L (GTA→TTA) EO946_RS06740sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base G (59/24);  new base T (0/36);  total (59/61)
Fisher's exact test for biased strand distribution p-value = 1.33e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 122 total aligned reads displayed.

ACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGACACATA  >  NZ_CP034943/1293214‑1293486
                                                                                                                                        |                                                                                                                                          
acggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaaa                                                                                                                                       >  2:866300/1‑140 (MQ=255)
acggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaaa                                                                                                                                       >  2:1654701/1‑140 (MQ=255)
             agaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGc                                                                                                                          >  2:113492/1‑140 (MQ=255)
              gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                                                         >  1:2083511/1‑140 (MQ=255)
              gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                                                         >  1:941543/1‑140 (MQ=255)
              gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGGAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                                                         >  1:1999924/1‑140 (MQ=255)
                ataagaCAGACAGACAATCAAAGAAGCTTTATCCCTGTTAA‑GGGG‑AATATAAAAACAAATGTTTTTGCTGCATAAGGTATAATGGTTTTCTTATCAACCAATCGCTGATCTTGTCGGTTTAAAAAAATCGACTGTGCaaa                                                                                                                       <  2:1994234/138‑1 (MQ=255)
                agaagaCAGAGAGCCAACCCACGAAGCTTTATCCCTTTTAC‑CGGG‑CAAACAAAAACAAATTTTTTTGCTGCATATGTTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCaaa                                                                                                                       <  2:2035230/140‑1 (MQ=255)
                  aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCCTCAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAATCg                                                                                                                     >  2:1245047/1‑140 (MQ=255)
                  aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                                     >  1:1976070/1‑140 (MQ=255)
                  aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                                     >  1:843649/1‑140 (MQ=255)
                  aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                                     >  2:502057/1‑140 (MQ=255)
                  aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCAGAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                                     >  1:2059867/1‑140 (MQ=255)
                         agagagaATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTTTGAACGAATCGCTGATCTTCTCGGGGTAAAAAAATCGAATGTGCAAACGACCATCa                                                                                                              >  2:1948500/1‑140 (MQ=255)
                         agagagaATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                                                              >  2:1432628/1‑140 (MQ=255)
                         agagagaATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                                                              >  2:1321510/1‑140 (MQ=255)
                         agagagaATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                                                              >  2:1134070/1‑140 (MQ=255)
                         agagagaATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                                                              >  2:2090319/1‑140 (MQ=255)
                         agagagaATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                                                              >  1:2056842/1‑140 (MQ=255)
                         agagagaATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                                                              >  2:69004/1‑140 (MQ=255)
                             agaATCCAAGACGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGACATGTTGTGGCTGCCTCAGGGAGTATGCTTTTGTGAGGAACGCATCGCTGAGCTGCTCGGTGTCCACAAATCGACTGTGCGACGGACCATCAGACg                                                                                                          >  2:1077288/1‑140 (MQ=255)
                               aaaacaacaaGCTTTATCCATGTTAA‑CGGG‑AACAAAAAAATAAATTTTTTTGCTTAATAAGTTATAATGATTTTATTATCAACCAATCGATGATTTTCTGGGTTAAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                                                                        <  1:1190208/136‑1 (MQ=255)
                               aaTCCAAGACGCGTTATCCCTTTTAA‑CGGG‑CAGAGAAAAAGAATTTTTTTTGCTGAATATGGTAGAATGATTTTCTTTTGAACGAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                                                                        <  2:410911/140‑1 (MQ=255)
                                aTCCAAGAAGCGTTACCCCTTTTAC‑CGGG‑CAGAGAAAAATAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACTAATCGCTGATCTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGc                                                                                                       <  2:938236/140‑1 (MQ=255)
                                       ccgCGTTA‑CCCCGTTAACCGGC‑CACAAAAAAACAAATTTTTTTGCTCCAAACGGTAGAAGGCTTTTCTTATCAACCAATCGCTGATCTTCGCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTa                                                                                                <  1:1814423/138‑1 (MQ=255)
                                       aagCTTCATCCCTTTTAA‑CGGG‑CAGAGAAAAACAAATGTTTTTGCTGCATAAGGTATAATGCTTTTATTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTa                                                                                                <  2:236743/140‑1 (MQ=255)
                                        agcttaaTCCCTTTTAA‑CGGC‑AAGAAAAAAACAAATTTTTTTGCTCCATAAGGTACAATGTTTTTGTTATCAACTAAGCGTTGATCTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaa                                                                                               <  1:1321510/134‑1 (MQ=255)
                                         cgtttACCCCTTTTAA‑GGGC‑AACACAAAAACAATTTTTTTTGCGCAATATGGTAGATTTTTTTTTTTATTAACTAATCGCTTATGTTCTGGTTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                                                              <  1:743071/137‑1 (MQ=255)
                                               tCCCTTTTAA‑CGGG‑AAGACAAAAAGAAATTTTTTTGCTTAATATGTTATATTGTTTTACTTATGAACTAATCGCTGATCTTCTGGGTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCa                                                                                        <  1:1265745/140‑1 (MQ=255)
                                                  cctttta‑CGGGCCAGATAAAAATAATTTTTTTTGCTGAATATGTTACATTGTTTTTGTTATAACCTAAGCGCTGATGTTGGGGGTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCaaa                                                                                      <  1:866300/134‑1 (MQ=255)
                                                   ttttAA‑CGGG‑CAGATAAAAAGAAATTTTTTTGCTGCATACGTTAGACTGGTTTTCTTATGAACTAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGa                                                                                    <  2:566931/138‑1 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTCAAGCTGCAAAgtc                                                                                   >  2:1214571/1‑138 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTCAAGATGCAAAGAc                                                                                   >  1:1664190/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  1:712649/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  2:1192484/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  2:266939/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  1:591207/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  1:566931/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  1:5138/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  2:712017/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  2:711595/1‑140 (MQ=255)
                                                    gTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAAAGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  2:630769/1‑140 (MQ=255)
                                                     ttAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAca                                                                                  >  1:1583687/1‑140 (MQ=255)
                                                       aa‑CGGG‑AATAAAAAAATAATTTTTTTTGCTGAATACGTTATAATGTTTTTCTTATTAACTAATCGCTTATCTTCTGGGTTTAAAAAACTCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAcagc                                                                                <  1:251931/140‑1 (MQ=255)
                                                            gg‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagga                                                                            >  2:2006121/1‑140 (MQ=255)
                                                            gg‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGAAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagga                                                                            >  2:1122333/1‑140 (MQ=255)
                                                                              gTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTGTCGGTGTAAAAAAATCGAGTGTGCAAACGACCATCAAACGGGCGATCGGAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGc                                                           >  2:282172/1‑140 (MQ=255)
                                                                              gTTTATGCTGCATAAGGTAGAATGATTTTCTTATGAAAGAATCACTGATATTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATTAAAATACAGCAGGAGGAAATGAATCGATCGc                                                           >  2:88533/1‑140 (MQ=255)
                                                                               tttttGCTGAATATGGTACAATGCTTTTATTATGAACGAATCGCTTATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt                                                          <  1:88533/140‑1 (MQ=255)
                                                                                       tcATACGGTAGAATGGTTTTCTTATCAACCAATCGCTTATCTTCTCGGTCAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGaa                                                  <  1:1117395/139‑1 (MQ=255)
                                                                                         atatGGTAGATTGATTTTCTTATTAACGAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAg                                                <  2:1264877/140‑1 (MQ=255)
                                                                                         aTACGGTAGAATGTTTTTTTTATCAACCAATCGCTGATCTTCTGGGGGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAg                                                <  1:113492/140‑1 (MQ=255)
                                                                                          tatgttAGAATGATTTTATTATGAACGAATCGCTTATCTTCTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc                                               <  1:1654701/135‑1 (MQ=255)
                                                                                          tatggTATAATGTTTTTATTATGAACGAATCGCTGATCTTCTGGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc                                               <  1:444752/137‑1 (MQ=255)
                                                                                          tacggTAGATTGCTTTTCTTATTAACGAATCGCTGATTTTCGGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc                                               <  1:1104311/137‑1 (MQ=255)
                                                                                                 caaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCAt                                        <  1:1406671/139‑1 (MQ=255)
                                                                                                  aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCACACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGACATGAATCGATCGCTTGCCTGAAAGCTTGTCATa                                       >  1:459089/1‑140 (MQ=255)
                                                                                                  aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa                                       >  1:1284470/1‑140 (MQ=255)
                                                                                                       ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGTCAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  2:1775515/1‑140 (MQ=255)
                                                                                                       ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  1:1004898/1‑140 (MQ=255)
                                                                                                       ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  1:948643/1‑140 (MQ=255)
                                                                                                       ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  2:670923/1‑140 (MQ=255)
                                                                                                       ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  1:342457/1‑140 (MQ=255)
                                                                                                       ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGACATACGttt                                  >  1:865551/1‑140 (MQ=255)
                                                                                                        ttttttATGACCTACTGGCTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:21995/140‑1 (MQ=255)
                                                                                                        ttttttATGAACTAATCGCTTATCTTCTGGGTGAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:108555/140‑1 (MQ=255)
                                                                                                        ttttttATGAACGAATCGATGATCTTTTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:1184113/140‑1 (MQ=255)
                                                                                                        tttCTTTTTAAGGAATCGCTTTTCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:2056842/140‑1 (MQ=255)
                                                                                                        tttCTTTTTAACTAATCGCTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAACATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:318751/140‑1 (MQ=255)
                                                                                                        tttCTTTTCAACGAATGGCTGATCTTCTCGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:1245047/140‑1 (MQ=255)
                                                                                                        tttCTTTTCAACGAATCGCTTTTTTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGATTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:1276859/140‑1 (MQ=255)
                                                                                                        tttCTTATTAACTAATCGCTTATTTTCTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:337838/140‑1 (MQ=255)
                                                                                                        tttCTTATTAACGAATGGCTGATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:1583687/140‑1 (MQ=255)
                                                                                                        tttCTTATTAACCCTTCGCTTTTCTTCTGGGTTTAAAAAAATCGACTGTTCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACTGCAGGAGGAAATGAATCGATCGCTTGCCTGAATGCTTGTCATACGTTTg                                 <  2:337902/140‑1 (MQ=255)
                                                                                                        tttCTTATGACAGACTCGCTTATTTTCTCGGTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:986553/140‑1 (MQ=255)
                                                                                                        tttCTTATGAACTAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:1809833/140‑1 (MQ=255)
                                                                                                        tttCTTATCAACGAATGGGTGATCTTATGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:1947133/140‑1 (MQ=255)
                                                                                                        tttCTTATCAACGAATCGCTTATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:1176965/140‑1 (MQ=255)
                                                                                                        tttCTTATCAACGAATCGCTTATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:474082/140‑1 (MQ=255)
                                                                                                         ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGCGGAAATTAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGc                                >  1:1666310/1‑140 (MQ=255)
                                                                                                         ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGACAGCTTGTCATACGTTTGc                                >  1:2099661/1‑140 (MQ=255)
                                                                                                         ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGc                                >  2:1861182/1‑140 (MQ=255)
                                                                                                         ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGAAAATACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGc                                >  2:346964/1‑140 (MQ=255)
                                                                                                          tCTTATGAACGAATCGCTGTTCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGcc                               <  2:1976070/140‑1 (MQ=255)
                                                                                                               tGAACGATTCGATTATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta                          <  1:2090319/140‑1 (MQ=255)
                                                                                                               tGAACGAATGGATGATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta                          <  2:1545967/140‑1 (MQ=255)
                                                                                                               tGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta                          <  1:1432628/140‑1 (MQ=255)
                                                                                                               tGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta                          <  1:1432636/140‑1 (MQ=255)
                                                                                                               tGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta                          <  1:1861182/140‑1 (MQ=255)
                                                                                                                  actattCGGTGATCTTCTGGGTTTAAAAAAATCGACTGTGAAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                       <  1:1948500/135‑1 (MQ=255)
                                                                                                                  actaaTCGTTTATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                       <  2:843649/137‑1 (MQ=255)
                                                                                                                  actaaTCGTTGATCTTCTTGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                       <  2:550628/137‑1 (MQ=255)
                                                                                                                  actaaTCGCTTATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                       <  1:439050/137‑1 (MQ=255)
                                                                                                                  aCGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                       <  1:1192484/140‑1 (MQ=255)
                                                                                                                       tggcttttcttctCTGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGaa                  <  2:1999924/133‑1 (MQ=255)
                                                                                                                              ttttctGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa           <  1:1366027/138‑1 (MQ=255)
                                                                                                                              tcttctCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa           <  1:986290/140‑1 (MQ=255)
                                                                                                                                     ggTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:1925846/111‑1 (MQ=255)
                                                                                                                                     ggTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 >  1:1925846/1‑111 (MQ=255)
                                                                                                                                       tgtAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGACACATa  >  1:1132420/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
ACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAA‑CGGG‑CAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGACACATA  >  NZ_CP034943/1293214‑1293486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑