Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9700GT28.0% 149.7 / 29.1 107Q206K (CAA→AAA) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (32/45);  new base T (29/1);  total (61/46)
Fisher's exact test for biased strand distribution p-value = 4.19e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108
                                                                                                                                           |                                                                                                                                          
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTg                                                                                                                                            >  2:1132110/1‑140 (MQ=255)
 tATTGTAAGGGACACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATAGTCTCTGATTTCTCCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGGCGTTTACATGCAGTTTTTGc                                                                                                                                           >  2:802836/1‑140 (MQ=255)
 tATTGTAAGGGAAACGGCAGCCTAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:1032202/140‑1 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:1461457/140‑1 (MQ=255)
   ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAATTTTTTTCaa                                                                                                                                         >  1:210044/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:1640376/134‑1 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          >  2:1640376/1‑134 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAatat                                                                                                                                    >  2:1510723/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAatat                                                                                                                                    >  2:950790/1‑140 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTc                                                                                                                               >  2:954686/1‑140 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCACGTCTCTTATTCTACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                            >  2:1877290/1‑140 (MQ=255)
                           aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                 <  1:1612125/140‑1 (MQ=255)
                                   ggCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  1:1875037/139‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATGTTTGCAAAATAGTGCTCACAGGGTTTTTCACGGTTTTCTCa                                                                                                       >  2:439900/1‑140 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                       <  2:467482/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                       <  2:651983/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                       <  1:1745921/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATGTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                       >  2:442063/1‑140 (MQ=255)
                                        aGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGACATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTg                                                                                                    <  2:2095385/140‑1 (MQ=255)
                                           aaaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                                 <  1:162454/140‑1 (MQ=255)
                                            aaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATaa                                                                                                >  2:2001141/1‑140 (MQ=255)
                                             aaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATAAATTTTTTCAAAAAATTTCTCAAAATTTTTTTAAACGGTTTATCAATGATaaa                                                                                               >  1:459941/1‑140 (MQ=255)
                                               ggCAAGTCTCTTATTATACTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTGCTCACATGGTTTTTCACGGTTTTCTAACTGATAAACa                                                                                             >  2:578082/1‑140 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                             >  2:1711548/1‑140 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                             >  2:490963/1‑140 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTTCAAAATATTTCTCACAAGGTTTTTAACGGTTTTGTCAATGATAAACa                                                                                             >  1:1974463/1‑140 (MQ=255)
                                               ggCAAGTCTCGTATTATACTATCTCATTTCTACCCAGCCATTTATAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGCAAAATATAGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                             >  2:2089938/1‑140 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  2:503459/140‑1 (MQ=255)
                                                   aGTCTCTTAGTAGACTAACGCATTTCTACCCAGCAATTTTTAATGGCGACCACCACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  2:1261705/140‑1 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                        >  2:288642/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCAAATGGTTTTTAACGGTTTTATAACTGATAAACAAAGAt                                                                                        >  2:302184/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAAATTGTCAAAAGGTTTTTAACGGTTTTATAACTGATAAAAAATTAt                                                                                        >  1:764485/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAAAATTTCTCACAAGGTTTTTCAAGGTTTTCTCACTTATAAAAAATTAt                                                                                        >  1:1575540/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTTCAAAATATTGCTCACAAGGTTTTTCACCGTTTTATAACTTATAAAAAATGAt                                                                                        >  2:1909820/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAAAATTTGTCCAATGGTTTTTAACCGTTTTATAAATGATAAAAAATGAt                                                                                        >  1:960309/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAAATTTTTTCAAAATATTGCTCACATGGTTTTTCACCGTTTTCTCACTGATAAAAAATGAt                                                                                        >  2:783537/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAAAATTTGTCAAATGGTTTTTAAAAGTTTTATCACTTATAAACAAAGAt                                                                                        >  1:1292569/1‑140 (MQ=255)
                                                          tattatACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCTGGTCGTTTACATTCATTTTTTGCAAAAAATTGCTAACAAGGTTTTTCACGGTTTTATCACTGATAAAAAAAGATTCaaac                                                                                  >  1:1732620/1‑137 (MQ=255)
                                                              ataCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCAAATGGTTTTTAACGGTTTTATCACATATAAACAAATATTAACCAAAAc                                                                              >  2:505028/1‑140 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTTCAAAAAAATTCTCACATTGTTTTTCAAGGTTTTATAAATTATAAAAAAATATTAACCaaaaac                                                                             >  1:395627/1‑136 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAAAATTTCTCACAATGTTTTTAACGGTTTTATCAATGATAAAAAAATATTCACCaaaaac                                                                             >  1:1722206/1‑136 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTTCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAATGATTCAACAATAAcgc                                                                           >  1:2036087/1‑140 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTTAAAAATATTTCTAACAATGTTTTTAACGGTTTTCTCACAGATAAACAATTATTCACCAATAAcgc                                                                           >  1:1394096/1‑140 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAATTTTTTTCAAAATATTTCTCACATTGTTTTTCACGGTTTTCTAACTTATAAAAAATGAATAACCAAAAAcgc                                                                           >  2:900768/1‑140 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGAGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAATATTGCTAACATGGTTTTTCAAGGTTTTCTAACAGATAAAAAAAGATTAAACAAAAAcgc                                                                           >  2:540345/1‑140 (MQ=255)
                                                                  tATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACcgcg                                                                          <  1:746819/140‑1 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAATGATTAACCAAAACCGCgg                                                                         >  2:871514/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTCTAAAATGGTTTTTCAAGGTTTTATCACAGATAAACAAAGATTCACCAAAAACGCgg                                                                         >  2:1424670/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCAATTTTTTCAAAAAATTGGTCAAAATTTTTTTAACGGTTTTATCAAAGATAAAAAATTATTAACAAAAACCACgg                                                                         >  1:1844753/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTTCTCACAAGGTTTTTCACGGTTTTATAAATGATAAAAAATGATTAAACAAAAACGCCGt                                                                        >  1:1432702/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTAACATTGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTCACCAATAACCCGGt                                                                        >  2:111261/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTTCAAAATATTTCTCACATGGTTTTTAAAGGTTTTGTAACTGATAAACAAAGATTAACCCATACCGCGGt                                                                        >  1:1241693/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCCAAAAATTTATAACATGGTTTTTCACGGTTTTATCACTGATAAACAATTATTCACCAAAAACGCGGt                                                                        >  2:1843146/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCAAAATGTTTTTCACCGTTTTATCACTGATAAAAAAAGATTAAACAAAAcccccgt                                                                        >  2:852763/1‑135 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATAAATTTTTTCAAAAAATTTCAAAAAATGTTTTTCACCGTTTTCTCACTGATAAACAAAGATTAACCAAAACAGCGGt                                                                        >  2:911346/1‑140 (MQ=255)
                                                                      tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTg                                                                      <  2:252296/140‑1 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCAATGATAAAAAAATATTAAACAAAACCGCGGTTGCtt                                                                   >  2:717351/1‑140 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTGACGGTTTTGTCACTGATAAAAAAAGATTCACCAAAAACGCGGTTGCTTTTTcc                                                              >  1:1766390/1‑140 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                              <  1:1510723/140‑1 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTAATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTGACGGGTTTGTGACTGATAAAAAAAGATTAACCAAAAACCCGGGTGGtttttgc                                                              >  1:528465/1‑138 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAATATTTCTGACATGGTTTTTCACGGTTTTGTGACTGATAAACCAAGGGTCACCCATACCCGGGTTGcgtgttac                                                              >  1:675011/1‑133 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGGAAAATAATGGTCACATGGGTTTTGACGGTTTTGTCACTGATAAACAATGTTTAACCCAAAACGCGGGTGGgtttttc                                                              >  1:219218/1‑138 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCAATTTTTGGAAAAAATTTCTCACATGGTTTTTCACGGTTTTCTGACTTTTAAACAATGATTAACCCAAACCGCGGTTGCgttttac                                                              >  1:2074111/1‑138 (MQ=255)
                                                                               cccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCg                                                             >  2:1186674/1‑140 (MQ=255)
                                                                                  aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAAGTCATTTTTTTCAAAATATTTCTCAAAAGGTTTTTAACGGTTTTGTGACTTATAAAAAATTATTAACCAAAACCCCGGTTGCGTTTTACGTCt                                                          >  1:452580/1‑140 (MQ=255)
                                                                                  aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTAACTGATAAACAATTATTAACCAATATAGCGGTTGCTTTTTACGTCt                                                          >  2:773554/1‑140 (MQ=255)
                                                                                  aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTCACCAATATCGCGGTTGCTTTTTCCGTCt                                                          >  2:773291/1‑140 (MQ=255)
                                                                                  aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTGTTTTCAAAATATTTCTCACATGGTTTTTAACGGTTTTCTCACTGATAAACAATTATTCACCACTAACGCGGGTGCTTTTTCCGTCt                                                          >  2:1602801/1‑140 (MQ=255)
                                                                                  aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAAAAAAGAATCACCAATACCGCGGTTGCTTTTTACGTCt                                                          >  2:545518/1‑140 (MQ=255)
                                                                                  aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCATTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACAGCGGTTGCTTTTTCCGTCt                                                          >  2:1937252/1‑140 (MQ=255)
                                                                                    ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAAGGTTTTTCACGGTTTTTTCACTGATAAACAATGATTCACCAATACCGCGGTTGGTTTTTCCGTCTGc                                                        >  1:2014592/1‑140 (MQ=255)
                                                                                    ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGc                                                        >  2:1814383/1‑140 (MQ=255)
                                                                                    ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCAGTCATTTTTTGCAAAAAATTGCTCCCATTGTTTTTCACCGTTTTCTCACTGATTAACAAATATGGACCAAAACCCAGGTTGGTTTTTACGTCttc                                                        >  1:1715099/1‑138 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  1:1927318/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                    <  2:141841/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                    <  2:141872/140‑1 (MQ=255)
                                                                                        tcttAATGGCGACCACAAAGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGCTTGCTTTTTCCGTCTGCAAGc                                                    <  2:1947982/138‑1 (MQ=255)
                                                                                                gctaCCACATAGGATTGAGTCCTGTCGTTTACATTCATTTTTTGAAAATTATTGCTCAAATTGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:1524644/137‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  2:459941/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:288642/140‑1 (MQ=255)
                                                                                                               tGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAAAGATTCACCAATACCGCGGTTGCGTTTTCAGTCTGCAAGCCTCTGCAGCACATAACATTCccg                             >  2:1762126/1‑140 (MQ=255)
                                                                                                               tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                             <  2:713191/140‑1 (MQ=255)
                                                                                                                 cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                           <  1:1711548/140‑1 (MQ=255)
                                                                                                                      ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                      <  1:1186674/140‑1 (MQ=255)
                                                                                                                       gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                     <  1:1909820/140‑1 (MQ=255)
                                                                                                                       gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                     <  2:1722206/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:210044/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCAATGATAAACAATGATTCACCAATACCGCGGTTGCTTGTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:1844753/140‑1 (MQ=255)
                                                                                                                           tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                 <  1:717351/140‑1 (MQ=255)
                                                                                                                                attcattTTTTTCAAAATATTGCTCAAATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTGCGTCTGCAAGCATCTGCAGCTCTTCACATTCCCGCCGAGTTAAAATATGTa            <  1:1806821/140‑1 (MQ=255)
                                                                                                                                attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa            <  1:950790/140‑1 (MQ=255)
                                                                                                                                          tGCATAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:1943066/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:1575540/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:764485/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:2001141/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:1451069/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  >  1:1211085/1‑140 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:903538/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCGGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:911346/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATAGTGCTCACATGGTTTTTCACGGTGTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATGCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:1345869/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: