Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9800GT25.7% 183.6 / 13.8 105S202R (AGC→AGA) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (31/47);  new base T (26/1);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 7.43e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 114 total aligned reads displayed.

AACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAT  >  NZ_CP034943/3446844‑3447119
                                                                                                                                        |                                                                                                                                           
aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTc                                                                                                                                          >  2:954686/1‑140 (MQ=255)
   ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCACGTCTCTTATTCTACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                                       >  2:1877290/1‑140 (MQ=255)
              aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                            <  1:1612125/140‑1 (MQ=255)
              aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                            <  1:1609834/140‑1 (MQ=255)
                        cccAGTAAAAGGCAAGTCTCTTATTATACTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATGTTTGCAAAATAGTGCTCACAGGGTTTTTCACGGTTTTCTCa                                                                                                                  >  2:439900/1‑140 (MQ=255)
                        cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                  <  2:651983/140‑1 (MQ=255)
                        cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                  <  2:467482/140‑1 (MQ=255)
                        cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                  <  1:1745921/140‑1 (MQ=255)
                              aaaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                                            <  1:162454/140‑1 (MQ=255)
                               aaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATaa                                                                                                           >  2:2001141/1‑140 (MQ=255)
                                aaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATAAATTTTTTCAAAAAATTTCTCAAAATTTTTTTAAACGGTTTATCAATGATaaa                                                                                                          >  1:459941/1‑140 (MQ=255)
                                  ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                                        >  2:1711548/1‑140 (MQ=255)
                                  ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                                        >  2:490963/1‑140 (MQ=255)
                                  ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTTCAAAATATTTCTCACAAGGTTTTTAACGGTTTTGTCAATGATAAACa                                                                                                        >  1:1974463/1‑140 (MQ=255)
                                  ggCAAGTCTCGTATTATACTATCTCATTTCTACCCAGCCATTTATAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGCAAAATATAGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                                        >  2:2089938/1‑140 (MQ=255)
                                      aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                    <  2:503459/140‑1 (MQ=255)
                                      aGTCTCTTAGTAGACTAACGCATTTCTACCCAGCAATTTTTAATGGCGACCACCACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                    <  2:1261705/140‑1 (MQ=255)
                                       gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                                   >  2:288642/1‑140 (MQ=255)
                                       gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCAAATGGTTTTTAACGGTTTTATAACTGATAAACAAAGAt                                                                                                   >  2:302184/1‑140 (MQ=255)
                                       gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAAAATTTCTCACAAGGTTTTTCAAGGTTTTCTCACTTATAAAAAATTAt                                                                                                   >  1:1575540/1‑140 (MQ=255)
                                       gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTTCAAAATATTGCTCACAAGGTTTTTCACCGTTTTATAACTTATAAAAAATGAt                                                                                                   >  2:1909820/1‑140 (MQ=255)
                                       gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAAAATTTGTCCAATGGTTTTTAACCGTTTTATAAATGATAAAAAATGAt                                                                                                   >  1:960309/1‑140 (MQ=255)
                                       gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAAATTTTTTCAAAATATTGCTCACATGGTTTTTCACCGTTTTCTCACTGATAAAAAATGAt                                                                                                   >  2:783537/1‑140 (MQ=255)
                                       gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAAAATTTGTCAAATGGTTTTTAAAAGTTTTATCACTTATAAACAAAGAt                                                                                                   >  1:1292569/1‑140 (MQ=255)
                                             tattatACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCTGGTCGTTTACATTCATTTTTTGCAAAAAATTGCTAACAAGGTTTTTCACGGTTTTATCACTGATAAAAAAAGATTCaaac                                                                                             >  1:1732620/1‑137 (MQ=255)
                                                 ataCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCAAATGGTTTTTAACGGTTTTATCACATATAAACAAATATTAACCAAAAc                                                                                         >  2:505028/1‑140 (MQ=255)
                                                  taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTTCAAAAAAATTCTCACATTGTTTTTCAAGGTTTTATAAATTATAAAAAAATATTAACCaaaaac                                                                                        >  1:395627/1‑136 (MQ=255)
                                                  taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAAAATTTCTCACAATGTTTTTAACGGTTTTATCAATGATAAAAAAATATTCACCaaaaac                                                                                        >  1:1722206/1‑136 (MQ=255)
                                                    cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATAACTGATAAAAAATGATTCACCAATAcccc                                                                                      >  2:746819/1‑138 (MQ=255)
                                                    cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTTCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAATGATTCAACAATAAcgc                                                                                      >  1:2036087/1‑140 (MQ=255)
                                                    cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTTAAAAATATTTCTAACAATGTTTTTAACGGTTTTCTCACAGATAAACAATTATTCACCAATAAcgc                                                                                      >  1:1394096/1‑140 (MQ=255)
                                                    cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAATTTTTTTCAAAATATTTCTCACATTGTTTTTCACGGTTTTCTAACTTATAAAAAATGAATAACCAAAAAcgc                                                                                      >  2:900768/1‑140 (MQ=255)
                                                    cTATCTCATTTCTACCCAGCCATTTTTAATGGAGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAATATTGCTAACATGGTTTTTCAAGGTTTTCTAACAGATAAAAAAAGATTAAACAAAAAcgc                                                                                      >  2:540345/1‑140 (MQ=255)
                                                     tATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACcgcg                                                                                     <  1:746819/140‑1 (MQ=255)
                                                      aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAATGATTAACCAAAACCGCgg                                                                                    >  2:871514/1‑140 (MQ=255)
                                                      aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTCTAAAATGGTTTTTCAAGGTTTTATCACAGATAAACAAAGATTCACCAAAAACGCgg                                                                                    >  2:1424670/1‑140 (MQ=255)
                                                      aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCAATTTTTTCAAAAAATTGGTCAAAATTTTTTTAACGGTTTTATCAAAGATAAAAAATTATTAACAAAAACCACgg                                                                                    >  1:1844753/1‑140 (MQ=255)
                                                       tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTTCAAAATATTTCTCACATGGTTTTTAAAGGTTTTGTAACTGATAAACAAAGATTAACCCATACCGCGGt                                                                                   >  1:1241693/1‑140 (MQ=255)
                                                       tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCCAAAAATTTATAACATGGTTTTTCACGGTTTTATCACTGATAAACAATTATTCACCAAAAACGCGGt                                                                                   >  2:1843146/1‑140 (MQ=255)
                                                       tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCAAAATGTTTTTCACCGTTTTATCACTGATAAAAAAAGATTAAACAAAAcccccgt                                                                                   >  2:852763/1‑135 (MQ=255)
                                                       tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATAAATTTTTTCAAAAAATTTCAAAAAATGTTTTTCACCGTTTTCTCACTGATAAACAAAGATTAACCAAAACAGCGGt                                                                                   >  2:911346/1‑140 (MQ=255)
                                                         tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTg                                                                                 <  2:252296/140‑1 (MQ=255)
                                                            tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCAATGATAAAAAAATATTAAACAAAACCGCGGTTGCtt                                                                              >  2:717351/1‑140 (MQ=255)
                                                                 aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTGACGGTTTTGTCACTGATAAAAAAAGATTCACCAAAAACGCGGTTGCTTTTTcc                                                                         >  1:1766390/1‑140 (MQ=255)
                                                                 aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                                         <  1:1510723/140‑1 (MQ=255)
                                                                 aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTAATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTGACGGGTTTGTGACTGATAAAAAAAGATTAACCAAAAACCCGGGTGGtttttgc                                                                         >  1:528465/1‑138 (MQ=255)
                                                                 aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAATATTTCTGACATGGTTTTTCACGGTTTTGTGACTGATAAACCAAGGGTCACCCATACCCGGGTTGcgtgttac                                                                         >  1:675011/1‑133 (MQ=255)
                                                                 aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGGAAAATAATGGTCACATGGGTTTTGACGGTTTTGTCACTGATAAACAATGTTTAACCCAAAACGCGGGTGGgtttttc                                                                         >  1:219218/1‑138 (MQ=255)
                                                                 aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCAATTTTTGGAAAAAATTTCTCACATGGTTTTTCACGGTTTTCTGACTTTTAAACAATGATTAACCCAAACCGCGGTTGCgttttac                                                                         >  1:2074111/1‑138 (MQ=255)
                                                                  cccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCg                                                                        >  2:1186674/1‑140 (MQ=255)
                                                                     aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAAGTCATTTTTTTCAAAATATTTCTCAAAAGGTTTTTAACGGTTTTGTGACTTATAAAAAATTATTAACCAAAACCCCGGTTGCGTTTTACGTCt                                                                     >  1:452580/1‑140 (MQ=255)
                                                                     aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTCACCAATATCGCGGTTGCTTTTTCCGTCt                                                                     >  2:773291/1‑140 (MQ=255)
                                                                     aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTGTTTTCAAAATATTTCTCACATGGTTTTTAACGGTTTTCTCACTGATAAACAATTATTCACCACTAACGCGGGTGCTTTTTCCGTCt                                                                     >  2:1602801/1‑140 (MQ=255)
                                                                     aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAAAAAAGAATCACCAATACCGCGGTTGCTTTTTACGTCt                                                                     >  2:545518/1‑140 (MQ=255)
                                                                     aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCATTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACAGCGGTTGCTTTTTCCGTCt                                                                     >  2:1937252/1‑140 (MQ=255)
                                                                       ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGc                                                                   >  2:1814383/1‑140 (MQ=255)
                                                                       ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCAGTCATTTTTTGCAAAAAATTGCTCCCATTGTTTTTCACCGTTTTCTCACTGATTAACAAATATGGACCAAAACCCAGGTTGGTTTTTACGTCttc                                                                   >  1:1715099/1‑138 (MQ=255)
                                                                        cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                  <  1:1927318/140‑1 (MQ=255)
                                                                           ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                               <  2:141841/140‑1 (MQ=255)
                                                                           ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                               <  2:141872/140‑1 (MQ=255)
                                                                           tcttAATGGCGACCACAAAGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGCTTGCTTTTTCCGTCTGCAAGc                                                               <  2:1947982/138‑1 (MQ=255)
                                                                                   gctaCCACATAGGATTGAGTCCTGTCGTTTACATTCATTTTTTGAAAATTATTGCTCAAATTGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                       <  1:1524644/137‑1 (MQ=255)
                                                                                   gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                                       <  2:459941/140‑1 (MQ=255)
                                                                                   gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                                       <  1:288642/140‑1 (MQ=255)
                                                                                   gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                                       <  1:1877290/140‑1 (MQ=255)
                                                                                                  tGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAAAGATTCACCAATACCGCGGTTGCGTTTTCAGTCTGCAAGCCTCTGCAGCACATAACATTCccg                                        >  2:1762126/1‑140 (MQ=255)
                                                                                                  tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                        <  2:713191/140‑1 (MQ=255)
                                                                                                         ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                 <  1:1186674/140‑1 (MQ=255)
                                                                                                          gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                <  1:1909820/140‑1 (MQ=255)
                                                                                                          gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                <  2:1722206/140‑1 (MQ=255)
                                                                                                            cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                              <  2:210044/140‑1 (MQ=255)
                                                                                                            cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTaaa                              <  2:1408515/140‑1 (MQ=255)
                                                                                                            cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCAATGATAAACAATGATTCACCAATACCGCGGTTGCTTGTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                              <  2:1844753/140‑1 (MQ=255)
                                                                                                              tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                            <  1:717351/140‑1 (MQ=255)
                                                                                                                   attcattTTTTTCAAAATATTGCTCAAATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTGCGTCTGCAAGCATCTGCAGCTCTTCACATTCCCGCCGAGTTAAAATATGTa                       <  1:1806821/140‑1 (MQ=255)
                                                                                                                   attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                       <  1:950790/140‑1 (MQ=255)
                                                                                                                   attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                       <  1:505028/140‑1 (MQ=255)
                                                                                                                             tGCATAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  2:1943066/140‑1 (MQ=255)
                                                                                                                             tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             >  1:1211085/1‑140 (MQ=255)
                                                                                                                             tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  2:764485/140‑1 (MQ=255)
                                                                                                                             tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  1:1451069/140‑1 (MQ=255)
                                                                                                                             tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  1:2001141/140‑1 (MQ=255)
                                                                                                                             tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  2:903538/140‑1 (MQ=255)
                                                                                                                             tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCGGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  1:911346/140‑1 (MQ=255)
                                                                                                                             tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  2:335257/140‑1 (MQ=255)
                                                                                                                             tGCAAAATAGTGCTCACATGGTTTTTCACGGTGTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATGCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  1:1345869/140‑1 (MQ=255)
                                                                                                                                  aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATATCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  1:773554/140‑1 (MQ=255)
                                                                                                                                  aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATATCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  1:773291/140‑1 (MQ=255)
                                                                                                                                  aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  1:1937252/140‑1 (MQ=255)
                                                                                                                                  aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  2:675011/140‑1 (MQ=255)
                                                                                                                                  aaTATTGCTCACAGGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  1:1223784/140‑1 (MQ=255)
                                                                                                                                       tGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGa   >  1:1992306/1‑140 (MQ=255)
                                                                                                                                        gCTCAGAGGGTTTGTCACGGCTTTCTCACAGATAAACAATGATTCACCAATACCGCGGTGGCTTTCTGCGTCCCCAACCATCTCCAGCACTTCACCTTCCCGCCGAGTTCAAATAGGTACTGGTCTGCGGATTTCAGGAt  <  2:1098787/140‑1 (MQ=255)
                                                                                                                                        gCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  1:676101/140‑1 (MQ=255)
                                                                                                                                        gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  2:1127763/140‑1 (MQ=255)
                                                                                                                                        gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  2:395627/140‑1 (MQ=255)
                                                                                                                                        gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  1:900768/140‑1 (MQ=255)
                                                                                                                                        gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  1:1424670/140‑1 (MQ=255)
                                                                                                                                        gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  2:1292569/140‑1 (MQ=255)
                                                                                                                                        gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt  <  1:578082/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
AACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAT  >  NZ_CP034943/3446844‑3447119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑