Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,851,2620CA22.1% 253.2 / 11.7 113W38L (TGG→TTG) EO946_RS19820hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (75/13);  new base A (0/25);  total (75/38)
Fisher's exact test for biased strand distribution p-value = 6.98e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

ATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGAT  >  NZ_CP034943/3851128‑3851399
                                                                                                                                      |                                                                                                                                         
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATCACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTCTTACCTGTATCAACATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                      >  2:370176/1‑140 (MQ=255)
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCaaaaa                                                                                                                                      >  1:790957/1‑140 (MQ=255)
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCacaaa                                                                                                                                      >  2:1540162/1‑140 (MQ=255)
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                      >  2:2068222/1‑140 (MQ=255)
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                      >  1:1047583/1‑140 (MQ=255)
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                      >  2:1547966/1‑140 (MQ=255)
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                      >  2:154164/1‑140 (MQ=255)
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCaaaaa                                                                                                                                      >  1:1250470/1‑140 (MQ=255)
aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                      >  2:632018/1‑140 (MQ=255)
  tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTCTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                                    >  1:1723665/1‑140 (MQ=255)
  tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAcc                                                                                                                                    >  1:94293/1‑139 (MQ=255)
  tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                                    >  1:1371084/1‑140 (MQ=255)
  tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                                    >  1:1469103/1‑140 (MQ=255)
         ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAc                                                                                                                             >  1:700635/1‑140 (MQ=255)
          gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACg                                                                                                                            >  1:780863/1‑140 (MQ=255)
                   tttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACAGTATCATTAATCGATAGTATGAAAGCCCTTACCAAAAACGATAAAACGACACTGcgt                                                                                                                   >  1:769977/1‑138 (MQ=255)
                       gCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGcttctta                                                                                                               >  1:1560016/1‑139 (MQ=255)
                            ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGtt                                                                                                          >  1:280310/1‑140 (MQ=255)
                              ttATCACCTTTTAACAATAACCTAAAATGTACTCGGACATTTATTCTAAAAATTAACCAGTCTTTTTTACCTTTATATTTATTCGACCGTATCAACGCCCTCCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCg                                                                                                        <  2:1355704/140‑1 (MQ=255)
                                aTCACCTTGTAACCATAACCTACAAAGTACTGGCACCGGTATACAATAATTCAACCAGTCTTTTTTACCTGTATCTTGATACGAGCGTATCCACGCCCTTCCAAAAAACAATAAAACGACTCTGCTTCTTCAAGTTCGCa                                                                                                      <  2:45924/140‑1 (MQ=255)
                                   aCCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGa                                                                                                   >  1:489040/1‑140 (MQ=255)
                                   aCCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGa                                                                                                   >  1:488894/1‑140 (MQ=255)
                                     cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                                 >  1:1086740/1‑140 (MQ=255)
                                           acaaTAACTTTCAATGTACTCGGCACGGTCTTATATAAATTAACACGTCGTTATTACATGTATATTGTTTCGCTCGTCTGCAACCCCTTCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaa                                                                                           <  2:213367/137‑1 (MQ=255)
                                               tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                       >  1:1501194/1‑140 (MQ=255)
                                               tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                       >  1:1468701/1‑140 (MQ=255)
                                               tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                       >  1:1829890/1‑140 (MQ=255)
                                               tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCGGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                       >  1:1000569/1‑140 (MQ=255)
                                                  cctaaaaaTTACTCGCACTGTTATTATATAAATGAACCAGGGTTTATTACCTGTATCATGATTCTATCGTATGCACGCCCTCCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:1723665/135‑1 (MQ=255)
                                                  ccTTCAACGTACGCGGACTGTTTTTATATAAATGACCCTGTGTTTATTACCTTTATCTTGATTCGATCGTAGGCACGCCCTCCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:700635/140‑1 (MQ=255)
                                                  ccTTCAAATTACGGGTATATGTATTATATAAATCACCCAGTTGTTTTTAACTGTATATTTTTTCGAGCGTATCCAACCCCTACCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:1423065/140‑1 (MQ=255)
                                                  ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatt                                                                                    >  1:752752/1‑139 (MQ=255)
                                                  ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    >  2:1572430/1‑140 (MQ=255)
                                                  ccTTCAAAGTACTCGGACAGGTATTATATAAATGAACCCGTCGTTATTCCCTGTATCATTATTCGATCGTCTGCACGCCCTGCCCAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:489040/140‑1 (MQ=255)
                                                  ccTTAAAAGTACTCGCACAGGTCTTCTATAAATTAACCAGTCGTTTTTACCTTTATCTTTATTCGATCTTTTGAAACCCCTTCCCAAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:1371084/140‑1 (MQ=255)
                                                  cattCAAATTAATCGGACAGTTCTTATATAATTGAACCATTCGTTTTTACATTAATAATTATTCTATCTTATGAAACCCCTTCCCAAAAACTTTAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:2026714/138‑1 (MQ=255)
                                                  catcaaaaGTACTCGCACAGTTATTCTATAAATTAACCAGTCTTTTTTCCCTTTATCTTGATTCGATCGTTTCCAAGCCCTCCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:408585/135‑1 (MQ=255)
                                                  cagacAAAGTACTCGGACAGGTCTTCTATAAATTAACCTGCCGTTATTACCTGTATCATTATTCTCTCGTATCCAAGCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:728264/136‑1 (MQ=255)
                                                       aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                               >  2:1523760/1‑140 (MQ=255)
                                                        atgTACTCGCACAGGTCTTATATAAATGAACCAGTCGTTTTTACCTTTATCATTATTCGATCGTATCAAAGCCCTACCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCtt                                                                              <  2:1047583/138‑1 (MQ=255)
                                                             cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACCATAAAACGACACTTCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTaatac                                                                         >  1:1414266/1‑137 (MQ=255)
                                                             cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATGGTATGCAAGCCCTTGCCAAAAACGATAAAACGACTCTGGTTCTTCACGTTCGCAAGTCATAACAACTTTATGAGGCTTAagag                                                                         >  1:271012/1‑140 (MQ=255)
                                                             cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAagag                                                                         >  1:405817/1‑140 (MQ=255)
                                                             cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                         >  1:645407/1‑140 (MQ=255)
                                                             cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                         >  1:2011910/1‑140 (MQ=255)
                                                             cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                         >  1:65350/1‑140 (MQ=255)
                                                             cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGGATCATGATTCGAACGTATGCAATCCCGTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGAAATAACAACTTTTTGTGGCTTAatag                                                                         >  1:1510460/1‑140 (MQ=255)
                                                                     ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGAAGAGGCTTAATTGCGATCATg                                                                 >  1:1403188/1‑140 (MQ=255)
                                                                     ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTAGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATAATg                                                                 >  1:1635363/1‑140 (MQ=255)
                                                                     ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAACCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                                 >  1:1635361/1‑140 (MQ=255)
                                                                        tttctATAAATCACCCTTTCTTTATTACGTTTTTCATTATTCTATCTTAGGCACCCCCTCCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCt                                                              <  2:1086740/139‑1 (MQ=255)
                                                                            tataAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                                          >  1:1814737/1‑140 (MQ=255)
                                                                               aattGAACCTGTCTTTTTTACCTTTATATTTATTCGGTGGTTTGAATGCCATTCCAAAAACCGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                                       <  2:671798/137‑1 (MQ=255)
                                                                               aactcaaCCCGTCTTTATTACCTGTTTCATGATTCGATCGTAGGCACGCCCTGCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                                       <  2:1712803/135‑1 (MQ=255)
                                                                                 aTGAACCAGTCGTTATTACCGGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGAATCTGCTTATTAAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTATAAATTGCCg                                                     >  1:649532/1‑140 (MQ=255)
                                                                                   taaCCAGGCTTGATTACGGGTATCTGGATTCCAGCGTATGCACCCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                                                   <  2:1091658/139‑1 (MQ=255)
                                                                                    caCCCGTGGTTATTCCCTTTATAATTTTTCGACCGTATCAAAGCCCTCCCAAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  1:1386732/139‑1 (MQ=255)
                                                                                    aaCCCGTCTTTTTTACCTTTATCTTGATTCGATCGTATGCAAGCCCTACCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  1:1414994/140‑1 (MQ=255)
                                                                                    aaCCATTCGTTATTACCTGTTTCTTGATTCGATCGTATGCAAGCCCTCCCCAAAACCTATACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  2:2084715/140‑1 (MQ=255)
                                                                                    aaCCAGTCTTTATTACCTGCATCTTGTTCCGATCGGCTGCAACCCCTGCCAAAAACCCAAACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  2:21434/140‑1 (MQ=255)
                                                                                        aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAACGATAAAACGACGCGGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCTTAAGAGCGATCCTGTCTAAATTGCCCATACTGc                                              >  1:934239/1‑140 (MQ=255)
                                                                                          tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAATGCCGATACTGCtt                                            >  1:2084715/1‑140 (MQ=255)
                                                                                                    cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCACGACATAACAACTTGATGAGGCTTAAGAGCGATCATGGCTAAATTGCCGATACTGCTTATAGCGGTTa                                  >  1:1609624/1‑140 (MQ=255)
                                                                                                    cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa                                  >  2:1803070/1‑140 (MQ=255)
                                                                                                     tGTATCATGAGTCGAGCGTATCCAACCCCTCCCAAAAACCGAAAAAACGACTCTGCTTCTTCCAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt                                 <  1:1437918/140‑1 (MQ=255)
                                                                                                      gTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt                                >  1:1172817/1‑140 (MQ=255)
                                                                                                        aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                              >  1:1636386/1‑140 (MQ=255)
                                                                                                        aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                              >  1:1114770/1‑140 (MQ=255)
                                                                                                         tCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTcc                             >  1:817099/1‑140 (MQ=255)
                                                                                                            tgttccGATCGTATGCAAGCCCTTCCAAAACACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                          <  1:1973070/135‑1 (MQ=255)
                                                                                                              aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                        >  2:1796085/1‑140 (MQ=255)
                                                                                                              aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                        >  2:1030271/1‑140 (MQ=255)
                                                                                                              aTTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAATTCCGATACTGCTTATAGCGGTTATTTCCATaaa                        >  2:1030386/1‑140 (MQ=255)
                                                                                                               ttCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaaa                       >  2:395862/1‑140 (MQ=255)
                                                                                                                 cGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCACGACCTAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTACATAAAATc                     >  1:58912/1‑140 (MQ=255)
                                                                                                                   aTCTTATGAAAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                   <  2:283186/140‑1 (MQ=255)
                                                                                                                   aTCGTTTCCAAGCCCTTCCAAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                   <  2:405817/140‑1 (MQ=255)
                                                                                                                   aTCGTATTAAAGCCCTTCCAAAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                   <  2:1510460/140‑1 (MQ=255)
                                                                                                                   aTCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTCTTTCCATAAAATCtt                   >  2:1374966/1‑140 (MQ=255)
                                                                                                                   aTCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCGTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                   >  1:1041322/1‑140 (MQ=255)
                                                                                                                    tCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTc                  >  2:1619440/1‑140 (MQ=255)
                                                                                                                      ttattaaaGCCCTTCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc                <  2:1414266/134‑1 (MQ=255)
                                                                                                                       tATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTcccc               >  2:774168/1‑140 (MQ=255)
                                                                                                                         tccacgCCCTCCCAAAAACCGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt             <  1:1743793/135‑1 (MQ=255)
                                                                                                                         tGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt             >  1:283186/1‑140 (MQ=255)
                                                                                                                         cgCACGCCCTACCCACAAACCACAACACGACTCTGCTTCATCAACTTCGCCAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATGTCCATAAAATCTTCCCCtt             <  1:1540162/139‑1 (MQ=255)
                                                                                                                            accccagtCCAAAAAACTATAACACGACTCTGCTTCTTCAACTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGCTACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATc          <  1:2068222/133‑1 (MQ=255)
                                                                                                                              gCCTTTCACAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        <  1:1113700/140‑1 (MQ=255)
                                                                                                                              gCCCTTCCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        <  1:1523760/140‑1 (MQ=255)
                                                                                                                              gCCCTTCCCAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        <  2:1000569/140‑1 (MQ=255)
                                                                                                                              gCCCTTACAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        <  1:1128246/140‑1 (MQ=255)
                                                                                                                              gCCATTCCAAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        <  2:2082885/140‑1 (MQ=255)
                                                                                                                                  ttCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTgg    >  1:1457804/1‑140 (MQ=255)
                                                                                                                                   tCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTgta   >  2:1594636/1‑138 (MQ=255)
                                                                                                                                   tCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa   >  1:249812/1‑140 (MQ=255)
                                                                                                                                   tCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa   >  2:736852/1‑140 (MQ=255)
                                                                                                                                   tCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa   >  2:128396/1‑140 (MQ=255)
                                                                                                                                   tCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa   <  2:1609624/140‑1 (MQ=255)
                                                                                                                                    cccAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTCTCAGTTGGAt  >  2:1198037/1‑140 (MQ=255)
                                                                                                                                    cccAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGAt  >  2:2083321/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                         
ATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGAT  >  NZ_CP034943/3851128‑3851399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: