Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	418,441	Δ1 bp	intergenic (‑20/+631)	RPPX_01715 ← / ← RPPX_01720	hypothetical protein/lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	418,441	0	A	.	100.0%	130.3 / NA	57	intergenic (‑20/+631)	RPPX_01715/RPPX_01720	hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (30/27);  total (30/27)

TGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTACTCCCGGGA  >  CP009974/418295‑418479                                                                                                                                                   |                                        tgttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc                                    <  1:1018003/151‑1 (MQ=255)  gttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGCACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc                                   >  2:5279530/1‑151 (MQ=255)   ttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa                                  <  2:2569647/151‑1 (MQ=255)   ttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa                                  <  2:1237100/151‑1 (MQ=255)   ttgctgttgctgttgcagttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa                                  <  1:1465799/151‑1 (MQ=14)    tgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTTTTCTTTATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGAAGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCag                                 >  2:642160/1‑150 (MQ=14)     gctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagg                                >  1:1899047/1‑149 (MQ=255)     gctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagg                                <  1:2234711/151‑3 (MQ=255)     gctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagg                                <  1:5681427/151‑3 (MQ=255)     gctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagg                                <  1:4395701/151‑3 (MQ=255)     gctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagg                                <  1:4396652/151‑3 (MQ=255)       tgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCaggat                              >  2:3438780/1‑147 (MQ=14)       tgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCGTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCaggat                              <  1:1326099/151‑5 (MQ=14)          tgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCGA‑GGATggg                          >  1:5564964/1‑151 (MQ=11)           gctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCATGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         >  1:719600/1‑151 (MQ=11)           gctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         <  2:952094/151‑1 (MQ=11)           gctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         >  1:719599/1‑151 (MQ=11)            gtgttgctgttgctgttggtgttgctgttgGTCTTGATCTTGATCTTGAGCTTGGTCTTGGTCTTCGCGGGTTGAGGGGGCGGAAGGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc                        <  2:1191044/150‑1 (MQ=11)             tgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGcc                       >  1:3746239/1‑151 (MQ=11)             tgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGcc                       >  2:1091091/1‑151 (MQ=14)             tgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGcc                       >  1:3746415/1‑151 (MQ=11)             tgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGcc                       >  2:3281926/1‑151 (MQ=11)              gttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCATGCGGAGGGAGGCGACGGGCATGGATGCCCGTCAAGGGCTG‑GGCCCC‑CGGATGGGGCCt                      >  1:5039877/1‑151 (MQ=11)               tttgtgttgttgttggtgttgctgttgATCTTGATCTTGATCTTGGTCTTGATCCTGATCTTCGCGACTTCAGGAGGCCGACCGTAGGCCTTGCGGGGTGAGCTGACGGGCATGGATGCCCGTAAAGCGCTG‑GGCCTCA‑GGATGGGGCCTg                     <  2:1731399/147‑1 (MQ=11)               ttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                     >  2:986998/1‑151 (MQ=14)               ttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                     >  1:2039567/1‑151 (MQ=11)                 gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                   >  1:6949868/1‑151 (MQ=14)                 gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                   <  1:339085/151‑1 (MQ=14)                 gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                   <  2:5886003/151‑1 (MQ=11)                  ctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAg                  >  2:4247677/1‑151 (MQ=14)                  ctgttgcggttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAg                  <  2:4444335/151‑1 (MQ=11)                   tgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgc                 >  1:6831435/1‑151 (MQ=14)                   tgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgc                 >  2:3126230/1‑151 (MQ=14)                   tgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgc                 >  2:3125933/1‑151 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                >  2:3985442/1‑151 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                >  2:2943914/1‑151 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                <  1:5852880/151‑1 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                <  1:4386604/151‑1 (MQ=14)                    gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGCTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGGCTGCAgcg                >  2:3103371/1‑151 (MQ=14)                      tgcggttgctgttgctggtgATCGTGATCTTGATCTGGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCGGAACGTAGGCGGTGCGGAGTGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑g             <  2:2936005/151‑1 (MQ=11)                       gctgttgctgttgctgttgctgttGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑gg            >  2:468216/1‑150 (MQ=11)                        ctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggt           <  1:5375384/151‑3 (MQ=14)                         tgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtc          <  1:5577764/151‑4 (MQ=11)                         tgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtc          <  2:405464/151‑4 (MQ=14)                          gttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcc         <  1:367173/151‑5 (MQ=11)                          gttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcc         <  1:5570652/151‑5 (MQ=11)                           ttgctgttgctgttgATCTTGATCTTGATCTTGATTTTGTTCTTGTTCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcct        >  2:1052753/1‑146 (MQ=11)                           ttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcct        >  1:4390948/1‑146 (MQ=11)                           ttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcct        >  1:1559972/1‑146 (MQ=11)                            tgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcctc       >  2:3794822/1‑145 (MQ=11)                                gttgctgttgATCTTGATCTTGATCTTTGTCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg    <  2:5841095/151‑1 (MQ=11)                                gttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg    <  2:5107856/151‑1 (MQ=11)                                gttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg    >  1:516381/1‑151 (MQ=11)                                 ttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCggg   >  1:5474394/1‑151 (MQ=11)                                 ttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCggg   >  2:5484361/1‑151 (MQ=11)                                  tgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATGTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGa  <  2:6532876/151‑1 (MQ=1)                                  tgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGa  <  1:5258385/151‑1 (MQ=1)                                                                                                                                                   |                                        TGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTACTCCCGGGA  >  CP009974/418295‑418479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑