Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,971,951 G→T S9S (TCC→TCA)  RPPX_17675 ← hemolysin D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,971,9510GT98.8% 1145.4 / NA 330S9S (TCC→TCA) RPPX_17675hemolysin D
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base T (141/183);  total (147/183)
Fisher's exact test for biased strand distribution p-value = 3.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

GTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGA  >  CP009974/3971801‑3972098
                                                                                                                                                      |                                                                                                                                                   
gTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCgggg                                                                                                                                                     >  2:2314054/1‑151 (MQ=255)
  aGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGa                                                                                                                                                   <  1:6328312/151‑1 (MQ=255)
  aGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGa                                                                                                                                                   <  2:3255797/151‑1 (MQ=255)
    gcgtcgtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAgg                                                                                                                                                 <  2:4006333/148‑1 (MQ=255)
     cATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAggg                                                                                                                                                <  1:4697542/151‑1 (MQ=255)
        cgtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGttt                                                                                                                                             <  1:1306483/151‑1 (MQ=255)
         gtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTg                                                                                                                                            >  1:1082857/1‑151 (MQ=255)
           gggtggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTc                                                                                                                                          <  2:3656657/150‑1 (MQ=255)
           cgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGCGGGTTTGTc                                                                                                                                          >  1:2935961/1‑151 (MQ=255)
            ggtggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCt                                                                                                                                         <  1:4200349/151‑1 (MQ=255)
             gtggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTCATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTa                                                                                                                                        >  2:6977128/1‑151 (MQ=255)
               ggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACg                                                                                                                                      <  2:4954259/151‑1 (MQ=255)
               ggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACg                                                                                                                                      <  1:1711339/151‑1 (MQ=255)
               ggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACg                                                                                                                                      <  1:1871828/151‑1 (MQ=255)
                gtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACgg                                                                                                                                     >  1:5735290/1‑151 (MQ=255)
                 tttCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGc                                                                                                                                    <  1:3525994/151‑1 (MQ=255)
                     cAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTGGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACa                                                                                                                                >  2:2675809/1‑151 (MQ=255)
                      aGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAt                                                                                                                               <  2:4669668/151‑1 (MQ=255)
                        aTAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTc                                                                                                                             >  1:4963120/1‑151 (MQ=255)
                          ggCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAt                                                                                                                           <  2:5345115/150‑1 (MQ=255)
                            cGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtgg                                                                                                                         >  1:7093799/1‑151 (MQ=255)
                             ggCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggt                                                                                                                        >  1:2119942/1‑151 (MQ=255)
                                 ggTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggttggt                                                                                                                    >  1:3633991/1‑151 (MQ=255)
                                       gAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCAt                                                                                                              >  2:5722712/1‑151 (MQ=255)
                                          ccAGTAGCTGGTGAAGGACAGCAGGGTGATTGCGGTCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTATTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtct                                                                                                           <  1:1658883/151‑1 (MQ=255)
                                            aGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGTGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCg                                                                                                         >  2:6327994/1‑151 (MQ=255)
                                              tAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGaa                                                                                                       <  1:5165639/151‑1 (MQ=255)
                                               aGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAAt                                                                                                      >  1:6805830/1‑151 (MQ=255)
                                               aGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAAt                                                                                                      <  2:2799155/151‑1 (MQ=255)
                                                gCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAAtt                                                                                                     <  2:5339256/151‑1 (MQ=255)
                                                 cTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTg                                                                                                    <  1:5407442/151‑1 (MQ=255)
                                                   ggTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGCTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGCATTggg                                                                                                  >  2:6081914/1‑151 (MQ=255)
                                                    gggAAGGGCAGGAGGGTGACTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCCTGGTTGGTTTCCCTTCTCGCATTGGGc                                                                                                 <  2:1784579/149‑1 (MQ=255)
                                                      gAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCct                                                                                               >  2:6423845/1‑151 (MQ=255)
                                                          gCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctgctg                                                                                           <  2:478597/151‑1 (MQ=255)
                                                             agcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGCGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCCTTCTCGAATTGGGCCTgctggct                                                                                        >  2:2006085/1‑151 (MQ=255)
                                                              gcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggctg                                                                                       >  1:2201038/1‑151 (MQ=255)
                                                              gcagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCCGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggctg                                                                                       <  2:5579157/151‑1 (MQ=255)
                                                               cagGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGt                                                                                      <  1:4452216/151‑1 (MQ=255)
                                                                agGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTc                                                                                     <  1:1012710/151‑1 (MQ=255)
                                                                   gTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGGTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGt                                                                                  >  2:1609569/1‑151 (MQ=255)
                                                                    tGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGATTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGtt                                                                                 >  2:164992/1‑151 (MQ=255)
                                                                     gATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTtg                                                                                >  2:3832605/1‑151 (MQ=255)
                                                                      aTTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTtgt                                                                               <  2:2903642/151‑1 (MQ=255)
                                                                       ttGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTtgtg                                                                              <  1:5330526/151‑1 (MQ=255)
                                                                           ggCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAg                                                                          >  2:4262571/1‑151 (MQ=255)
                                                                               cggCTGCCGGTGGCGATGTTTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCAc                                                                      <  2:2415686/150‑1 (MQ=255)
                                                                               aGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCAc                                                                      >  2:814905/1‑151 (MQ=255)
                                                                               aGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGCTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCAc                                                                      >  2:6807430/1‑151 (MQ=255)
                                                                                 gCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTg                                                                    <  1:6437224/151‑1 (MQ=255)
                                                                                  gtGCCGCGGGCGCTGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGt                                                                   <  2:3127338/150‑1 (MQ=255)
                                                                                   tGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGCATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTc                                                                  <  1:4589858/151‑1 (MQ=255)
                                                                                   tGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTc                                                                  >  1:4031219/1‑151 (MQ=255)
                                                                                   tGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTc                                                                  >  2:332210/1‑151 (MQ=255)
                                                                                     ccGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCtt                                                                >  1:568527/1‑151 (MQ=255)
                                                                                       gCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGc                                                              >  1:3257829/1‑151 (MQ=255)
                                                                                       gCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGc                                                              >  1:810587/1‑151 (MQ=255)
                                                                                         tGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCGTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTc                                                            >  2:4511304/1‑151 (MQ=255)
                                                                                         tGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTc                                                            <  1:4551784/151‑1 (MQ=255)
                                                                                           gCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGa                                                          <  1:4767694/151‑1 (MQ=255)
                                                                                             gatgatTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGAtt                                                        <  2:6258451/151‑1 (MQ=255)
                                                                                             gatgatTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGAtt                                                        <  2:1097226/151‑1 (MQ=255)
                                                                                               ggatGGCGAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACGTTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTc                                                      <  2:6625706/150‑1 (MQ=255)
                                                                                                  ttGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTaa                                                   <  1:7167078/151‑1 (MQ=255)
                                                                                                  ttGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTaa                                                   <  1:3066076/151‑1 (MQ=255)
                                                                                                   tGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTaaa                                                  >  2:5129256/1‑151 (MQ=255)
                                                                                                      gaGGGGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGc                                               <  2:5813048/150‑1 (MQ=255)
                                                                                                      cAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGc                                               >  1:3810395/1‑151 (MQ=255)
                                                                                                        ggcggcGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTg                                             <  1:2181842/151‑1 (MQ=255)
                                                                                                         gcggcgACGGGTTTTAGCGCTCGGCCGGTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTgg                                            <  2:2226538/151‑1 (MQ=255)
                                                                                                          cggcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTTAAAGCTGgc                                           <  1:4340208/151‑1 (MQ=255)
                                                                                                          cggcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGgc                                           <  2:1302829/151‑1 (MQ=255)
                                                                                                            gcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGgcgc                                         <  2:7045465/151‑1 (MQ=255)
                                                                                                            gcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGgcgc                                         <  1:3762363/151‑1 (MQ=255)
                                                                                                              gACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGa                                       >  2:2884789/1‑151 (MQ=255)
                                                                                                              gACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGa                                       >  1:766640/1‑151 (MQ=255)
                                                                                                               aCGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGAt                                      >  1:974374/1‑151 (MQ=255)
                                                                                                                cGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATc                                     >  2:349218/1‑151 (MQ=255)
                                                                                                                  ggTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCg                                   >  1:3547296/1‑151 (MQ=255)
                                                                                                                       tAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTgg                              <  1:3134837/151‑1 (MQ=255)
                                                                                                                       tAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTgg                              <  2:3983803/151‑1 (MQ=255)
                                                                                                                       tAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGATTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTgg                              <  2:6923885/151‑1 (MQ=255)
                                                                                                                          cgcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAg                           >  2:3442650/1‑151 (MQ=255)
                                                                                                                            ctgggCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCg                         <  1:2675809/148‑1 (MQ=255)
                                                                                                                            cTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCg                         >  1:2415996/1‑151 (MQ=255)
                                                                                                                              cGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCggg                       <  1:7030629/151‑1 (MQ=255)
                                                                                                                              cGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCggg                       <  2:1457885/151‑1 (MQ=255)
                                                                                                                               ggCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGc                      <  2:2119942/151‑1 (MQ=255)
                                                                                                                               ggCCGCTCTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCACCATTCATGGTTGGTTTCCATTCTCCAATTGGGCCTGCTGGCTGTCGGTTGTGGTAGCCACTGTCTTGCTCGATTTCTAAGAGCGGGCGCGATCCCTGTGGGAGCAGGc                      <  2:441089/151‑1 (MQ=38)
                                                                                                                                 ccGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCtt                    <  2:1226205/151‑1 (MQ=255)
                                                                                                                                   gCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGc                  <  1:5611193/151‑1 (MQ=255)
                                                                                                                                    cTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGcc                 >  1:697618/1‑151 (MQ=255)
                                                                                                                                    cTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGcc                 <  2:6747901/151‑1 (MQ=255)
                                                                                                                                      gTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcg               <  1:6079650/151‑1 (MQ=255)
                                                                                                                                        tCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcgcg             <  1:116584/151‑1 (MQ=255)
                                                                                                                                        tCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcgcg             >  2:3754428/1‑151 (MQ=255)
                                                                                                                                         cAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGa            >  1:1452407/1‑151 (MQ=255)
                                                                                                                                           gTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAac          <  2:766640/151‑1 (MQ=255)
                                                                                                                                            tttCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAaca         >  1:4632343/1‑151 (MQ=255)
                                                                                                                                             ttCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAacac        <  1:2819060/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGA  >  CP009974/3971801‑3972098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑