Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,147,209	A→G	intergenic (+461/‑560)	RPPX_18425 → / → RPPX_18430	enoyl‑CoA hydratase/conjugal transfer protein TraR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,147,209	0	A	G	100.0%	122.0 / NA	75	intergenic (+461/‑560)	RPPX_18425/RPPX_18430	enoyl‑CoA hydratase/conjugal transfer protein TraR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/42);  total (33/42)

GGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTC‑‑‑‑‑‑‑GCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAAGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAG  >  CP009974/4147068‑4147353                                                                                                                                                                          |                                                                                                                                                 ggCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGt                                                                                                                                                <  2:5129007/151‑1 (MQ=11) ggCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGt                                                                                                                                                <  1:5842868/151‑1 (MQ=11) ggCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGt                                                                                                                                                <  1:6714052/151‑1 (MQ=11) ggCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGt                                                                                                                                                >  1:3114477/1‑151 (MQ=11) ggCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGt                                                                                                                                                >  2:1362122/1‑151 (MQ=11) ggCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGt                                                                                                                                                <  2:1937474/151‑1 (MQ=11)  gCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTg                                                                                                                                               <  1:5055703/151‑1 (MQ=11)   ggAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTgg                                                                                                                                              <  2:572734/150‑1 (MQ=11)   cGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTgg                                                                                                                                              <  2:1611035/151‑1 (MQ=11)    gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                                                                                                                             <  2:5151044/151‑1 (MQ=11)    gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                                                                                                                             <  1:579043/151‑1 (MQ=11)    gAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgc                                                                                                                                             >  2:2456479/1‑151 (MQ=11)     aaaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcg                                                                                                                                            <  1:2278394/151‑1 (MQ=11)     aaaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcg                                                                                                                                            <  1:2278108/151‑1 (MQ=11)      aaaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGgcgc                                                                                                                                           >  1:5857169/1‑151 (MQ=11)       aaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCa                                                                                                                                          <  1:3945938/151‑1 (MQ=11)       aaGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCa                                                                                                                                          <  2:3019376/151‑1 (MQ=11)        aGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCAt                                                                                                                                         >  2:6098041/1‑151 (MQ=11)        aGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCAt                                                                                                                                         <  1:6893248/151‑1 (MQ=11)        aGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCAt                                                                                                                                         <  2:6224217/151‑1 (MQ=11)         ggTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATa                                                                                                                                        <  2:5256904/151‑1 (MQ=11)           tGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGt                                                                                                                                      <  2:1538804/151‑1 (MQ=11)            gAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTc                                                                                                                                     <  2:3662464/151‑1 (MQ=11)            gAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTc                                                                                                                                     <  2:2378648/151‑1 (MQ=11)                cAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTTACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCt                                                                                                                                 >  2:4780773/1‑151 (MQ=11)                cAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCt                                                                                                                                 >  2:3419963/1‑151 (MQ=11)                 aGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGCGGCGCATAGTCACCtt                                                                                                                                >  2:4822496/1‑151 (MQ=11)                  gcgcCGGCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCCCCttt                                                                                                                               <  2:7166930/151‑1 (MQ=11)                  gCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCttt                                                                                                                               <  1:2587751/151‑1 (MQ=11)                  gCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCttt                                                                                                                               <  2:2678919/151‑1 (MQ=11)                   cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACTGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                                                                                                                              >  1:4584734/1‑151 (MQ=11)                   cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                                                                                                                              <  1:536192/151‑1 (MQ=11)                   cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                                                                                                                              >  2:3660685/1‑151 (MQ=11)                   cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                                                                                                                              <  1:5708947/151‑1 (MQ=11)                   cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                                                                                                                              >  2:858031/1‑151 (MQ=11)                   cGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTc                                                                                                                              >  1:433390/1‑151 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCTCAGGCGGCGCATAGTCATCTTTcc                                                                                                                             >  2:4254189/1‑151 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                                                                                                                             >  2:4031351/1‑151 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                                                                                                                             >  2:4031350/1‑151 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                                                                                                                             <  2:1972522/151‑1 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                                                                                                                             >  2:4254280/1‑151 (MQ=11)                    gTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTcc                                                                                                                             <  1:3677014/151‑1 (MQ=11)                     tCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCg                                                                                                                            <  2:2694026/151‑1 (MQ=11)                     tCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCg                                                                                                                            <  2:669843/151‑1 (MQ=11)                      cGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGc                                                                                                                           <  2:1486782/151‑1 (MQ=11)                       gCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGcc                                                                                                                          <  1:550565/151‑1 (MQ=11)                        ccATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGccc                                                                                                                         >  1:4220894/1‑151 (MQ=11)                        ccATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGccc                                                                                                                         >  1:6974384/1‑151 (MQ=11)                         cATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCt                                                                                                                        >  2:1686898/1‑151 (MQ=11)                          aTCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCtt                                                                                                                       <  2:4807653/151‑1 (MQ=11)                          aTCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCtt                                                                                                                       <  2:3830703/151‑1 (MQ=11)                          aTCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCtt                                                                                                                       <  2:3282704/151‑1 (MQ=11)                          aTCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCtt                                                                                                                       <  2:3133478/151‑1 (MQ=11)                          aTCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCtt                                                                                                                       <  1:3819241/151‑1 (MQ=11)                           tCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTa                                                                                                                      >  1:3049261/1‑151 (MQ=11)                           tCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTa                                                                                                                      >  1:4667032/1‑151 (MQ=11)                           tCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTa                                                                                                                      >  2:187983/1‑151 (MQ=11)                            cGCAAATGGACTGTTCACTGAGCCAAAACAGGCGAGTCC‑‑‑‑‑‑‑GCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCTTGCTTCCTG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAc                                                                                                                     <  2:5667140/151‑1 (MQ=11)                            cGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAc                                                                                                                     <  2:772871/151‑1 (MQ=11)                             gCAAATGGACTGTTCACTGAGCCAAAACAGACTAGTTC‑‑‑‑‑‑‑GCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TGCTTCTTTCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCTCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAct                                                                                                                    >  1:3320568/1‑150 (MQ=11)                             gCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcg                                                                                                                    >  2:248637/1‑151 (MQ=11)                             gCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcg                                                                                                                    >  1:3758972/1‑151 (MQ=11)                             gCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcg                                                                                                                    >  1:3760411/1‑151 (MQ=11)                               aaaTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcggc                                                                                                                  <  1:5633434/151‑1 (MQ=11)                                aaTGGACTGTTCACTGAGCCAAAACAGACGAGTTC‑‑‑‑‑‑‑GCTTCTTGCTTCTTTCTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTTTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTTACTTTTCCTCCCTTAcgtcg                                                                                                                 >  2:1786107/1‑151 (MQ=11)                                 aTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTActgccg                                                                                                                >  1:1874780/1‑149 (MQ=11)                                 aTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcggcgg                                                                                                                >  1:1455671/1‑151 (MQ=11)                                 aTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcggcgg                                                                                                                <  1:3452437/151‑1 (MQ=11)                                 aTGGACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTAcggcgg                                                                                                                >  1:1456209/1‑151 (MQ=11)                                   ggACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGt                                                                                                              <  2:251978/151‑1 (MQ=11)                                   ggACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGt                                                                                                              >  2:6032167/1‑151 (MQ=11)                                   ggACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGt                                                                                                              <  1:5357451/151‑1 (MQ=11)                                   ggACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGt                                                                                                              <  2:6445038/151‑1 (MQ=11)                                    gACTGTTCACTGAGCCAAAACAGACGAGTTCGCTTCTTGCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCGCGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTc                                                                                                             >  1:4384578/1‑151 (MQ=11)                                                                                                                                                                    cgcGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTACCCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTACGCTCCGCTCCGGGGTCCCCTCCCTACCGTCTTGCTCCCGGCAg  >  2:4330472/3‑151 (MQ=1)                                                                                                                                                                          |                                                                                                                                                 GGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAATGGACTGTTCACTGAGCCAAAACAGACGAGTTC‑‑‑‑‑‑‑GCTTCTTG‑‑‑‑‑‑‑CTTCTTGCTTCTTGCTTCT‑‑‑‑‑‑‑TG‑‑‑‑‑‑‑CTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAAGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAG  >  CP009974/4147068‑4147353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑