| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009974 | 4,147,342 | T→C | intergenic (+594/‑427) | RPPX_18425 → / → RPPX_18430 | enoyl‑CoA hydratase/conjugal transfer protein TraR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009974 | 4,147,342 | 0 | T | C | 83.7% | 48.3 / 9.3 | 43 | intergenic (+594/‑427) | RPPX_18425/RPPX_18430 | enoyl‑CoA hydratase/conjugal transfer protein TraR |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base C (12/24); total (17/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.34e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CACGCAAGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAAC > CP009974/4147203‑4147492 | cgcGCAGGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTACCCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTACGCTCCGCTCCGGGGTCCCCTCCCTACCGTCTTGCTCCCGGCAg > 2:4330472/3‑151 (MQ=1) tgcggctgGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAg < 1:1145241/149‑1 (MQ=14) cggcggGCCACTTTCTGTCAACCGCTACAAAGAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAgcg < 1:3792369/151‑1 (MQ=255) aCCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCAGCGTGGGGCCTCAGCGCTTGACGGGCAGCCATGCCCGTCACCTCCTTCCGCa > 1:1722551/1‑151 (MQ=9) aaaagaCGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACCGGCATCCATGCCCGTCACCTCCGTCCGCAAgg > 2:5984348/1‑151 (MQ=9) gcgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatc < 2:5022751/151‑1 (MQ=11) gcgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatc < 1:6372036/151‑1 (MQ=11) ctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaag < 2:978535/151‑1 (MQ=11) tCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga < 1:2924348/151‑1 (MQ=11) tCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga > 1:4724072/1‑151 (MQ=11) cGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatc < 1:2198158/151‑1 (MQ=11) ggggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatca < 2:4748457/151‑1 (MQ=11) ggggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatca < 2:3150480/151‑1 (MQ=11) gggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaa > 1:509570/1‑151 (MQ=11) gggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCCCCTGAAGTCGCGaagatcaagatcaagatcaa > 1:3368341/1‑151 (MQ=11) ggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaag < 1:6215650/151‑1 (MQ=11) gTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGacgatcaagatcaagatcaaga > 1:6024456/1‑151 (MQ=11) ccccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGTAGTCGCGaagatcaagatcaacatcacgatc > 2:5677355/1‑151 (MQ=11) ccccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatc < 1:2746657/151‑1 (MQ=14) cccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatca < 2:2662294/151‑1 (MQ=14) ccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaa < 1:4511196/151‑1 (MQ=11) ccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaa < 2:1799094/151‑1 (MQ=14) ctctctCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaag < 2:5230052/151‑1 (MQ=11) cctccTCCGGCCCCGCTCCCGGGGGGACCGCCCGGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaag < 2:4347091/147‑1 (MQ=11) tctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaaga < 1:576740/148‑1 (MQ=14) tcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatc < 2:4950639/150‑1 (MQ=14) tcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatc < 2:4573432/150‑1 (MQ=14) tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGTGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa < 2:5571221/151‑1 (MQ=14) tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCTCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGCCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa > 2:3547704/1‑151 (MQ=11) tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa < 1:3390318/151‑1 (MQ=14) tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa < 1:3390628/151‑1 (MQ=14) tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa < 1:1979591/151‑1 (MQ=14) tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagaccaa > 2:5312731/1‑151 (MQ=11) tCCGGCCTCGCTCCCGGGAGGACAGCGCTGCAAGCCCCATCCTGGGGCC‑CAGCGCTTGACGGCCATCCATGCCCGTCACCTCCCTCCGCAAGACCTACTTTCGGCCTCTTGAAGTCGCGAAgagcaagaacacgatcaagaccaagatcaa > 2:4509884/1‑151 (MQ=11) cccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa < 2:4632545/150‑1 (MQ=11) ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag > 1:1371004/1‑150 (MQ=11) ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag > 2:5137892/1‑150 (MQ=11) ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag < 2:1914768/151‑2 (MQ=11) ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat < 2:2513662/151‑4 (MQ=11) ccTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgctcaagatcaagctcaagatcaagatcaagatca > 1:5286940/1‑146 (MQ=11) ccTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagatca > 2:1875734/1‑146 (MQ=11) cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca > 1:4079021/1‑151 (MQ=0) cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca > 1:2542996/1‑151 (MQ=11) ttGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaa < 2:536192/151‑1 (MQ=9) ttGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaa > 1:3994264/1‑151 (MQ=2) tGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaac < 2:3819241/151‑1 (MQ=17) tGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaac > 2:2087134/1‑151 (MQ=9) | CACGCAAGTGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAAC > CP009974/4147203‑4147492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |