Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,147,431 A→G intergenic (+683/‑338) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,147,4310AG80.5% 447.3 / 97.6 185intergenic (+683/‑338)RPPX_18425/RPPX_18430enoyl‑CoA hydratase/conjugal transfer protein TraR
Reads supporting (aligned to +/- strand):  ref base A (12/24);  new base G (97/52);  total (109/76)
Fisher's exact test for biased strand distribution p-value = 6.54e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 185 total aligned reads displayed.

GCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTGGGG  >  CP009974/4147314‑4147577
                                                                                                                     |                                                                                                                                                  
gcgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatc                                                                                                                  <  2:5022751/151‑1 (MQ=11)
   ctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaag                                                                                                               <  2:978535/151‑1 (MQ=11)
        gggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCCCCTGAAGTCGCGaagatcaagatcaagatcaa                                                                                                          >  1:3368341/1‑151 (MQ=11)
         ggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaag                                                                                                         <  1:6215650/151‑1 (MQ=11)
          gTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGacgatcaagatcaagatcaaga                                                                                                        >  1:6024456/1‑151 (MQ=11)
            ccccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGTAGTCGCGaagatcaagatcaacatcacgatc                                                                                                      >  2:5677355/1‑151 (MQ=11)
              ccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaa                                                                                                    <  1:4511196/151‑1 (MQ=11)
               cctccTCCGGCCCCGCTCCCGGGGGGACCGCCCGGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaag                                                                                                   <  2:4347091/147‑1 (MQ=11)
                tctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaaga                                                                                                  <  1:576740/148‑1 (MQ=14)
                    tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCTCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGCCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa                                                                                              >  2:3547704/1‑151 (MQ=11)
                    tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa                                                                                              <  1:1979591/151‑1 (MQ=14)
                    tCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa                                                                                              <  1:3390318/151‑1 (MQ=14)
                    tCCGGCCTCGCTCCCGGGAGGACAGCGCTGCAAGCCCCATCCTGGGGCC‑CAGCGCTTGACGGCCATCCATGCCCGTCACCTCCCTCCGCAAGACCTACTTTCGGCCTCTTGAAGTCGCGAAgagcaagaacacgatcaagaccaagatcaa                                                                                              >  2:4509884/1‑151 (MQ=11)
                    cccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaa                                                                                              <  2:4632545/150‑1 (MQ=11)
                     ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag                                                                                             >  2:5137892/1‑150 (MQ=11)
                     ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag                                                                                             >  1:1371004/1‑150 (MQ=11)
                       ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                                           <  2:2513662/151‑4 (MQ=11)
                         ccTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgctcaagatcaagctcaagatcaagatcaagatca                                                                                         >  1:5286940/1‑146 (MQ=11)
                          cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                                         >  1:2542996/1‑151 (MQ=11)
                           ttGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaa                                                                                        <  2:536192/151‑1 (MQ=9)
                           ttGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaa                                                                                        >  1:3994264/1‑151 (MQ=2)
                            tGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaac                                                                                       >  2:2087134/1‑151 (MQ=9)
                              cTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacgg                                                                                     >  2:529362/1‑151 (MQ=18)
                              cTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacgg                                                                                     >  1:2373037/1‑151 (MQ=18)
                              cTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacgg                                                                                     >  2:2463064/1‑151 (MQ=18)
                                cccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggca                                                                                   >  1:2507661/1‑151 (MQ=25)
                                 ccGGCAGGACCGCGCCGAAGGCCCCATCCTGGTGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaa                                                                                  >  2:3461914/1‑151 (MQ=255)
                                 ccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaa                                                                                  >  1:1457984/1‑151 (MQ=255)
                                  cGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaac                                                                                 >  1:6443781/1‑151 (MQ=255)
                                  cGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaac                                                                                 <  2:3153434/151‑1 (MQ=255)
                                  cGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaac                                                                                 <  1:1196925/151‑1 (MQ=255)
                                    gCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacgg                                                                               >  2:500907/1‑151 (MQ=255)
                                     cAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggc                                                                              >  2:3468765/1‑151 (MQ=255)
                                        gACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaac                                                                           <  2:5534885/151‑1 (MQ=255)
                                         aCCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaacg                                                                          >  1:3889549/1‑151 (MQ=255)
                                            gcgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAacggcaacggccacggcaacggca                                                                       >  2:1729734/1‑151 (MQ=255)
                                             cgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAcggcaacggcaacggcaacggcaa                                                                      >  2:2643089/1‑151 (MQ=255)
                                              gcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACggcaacggcaacggcaacggcaac                                                                     >  1:79922/1‑151 (MQ=255)
                                              gcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACggcaacggcaacggcaacggcaac                                                                     >  1:6724791/1‑151 (MQ=255)
                                               ccGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaaca                                                                    >  2:2608531/1‑151 (MQ=255)
                                                cGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacag                                                                   >  1:3936768/1‑151 (MQ=255)
                                                  aaGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagca                                                                 >  2:6834676/1‑151 (MQ=255)
                                                    ggCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaac                                                               >  1:3083618/1‑151 (MQ=255)
                                                    ggCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaac                                                               >  1:563528/1‑151 (MQ=255)
                                                     gCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaaca                                                              >  1:1033311/1‑151 (MQ=255)
                                                      ccccATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGcaacagcaacag                                                             >  1:1803364/1‑151 (MQ=255)
                                                       cccATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCaacagcaacagc                                                            <  1:2138855/151‑1 (MQ=255)
                                                        ccATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAacagcaacagca                                                           <  1:7116334/151‑1 (MQ=255)
                                                         cATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAAcagcag                                                          <  2:2794409/151‑1 (MQ=255)
                                                          aTCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAgg                                                         >  1:7052517/1‑151 (MQ=255)
                                                           tCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGc                                                        >  1:86051/1‑151 (MQ=255)
                                                            ccTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGACGTCGCGAAGATCAAGATCAAGAACAAGATCAAGATCAACGGCACCGGCAACGGCAACGGCAACAGCAACAGCATGcc                                                       >  1:3549639/1‑150 (MQ=14)
                                                            ccTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCg                                                       <  2:1351523/151‑1 (MQ=255)
                                                             cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                                      <  1:3287347/151‑1 (MQ=255)
                                                                 ggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATgg                                                  <  1:108483/151‑1 (MQ=255)
                                                                   ccTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGc                                                >  1:4858185/1‑151 (MQ=255)
                                                                    cTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCa                                               >  1:244884/1‑151 (MQ=255)
                                                                        gcgcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGc                                           >  2:4058476/1‑151 (MQ=255)
                                                                           cTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAg                                        <  1:4068011/151‑1 (MQ=255)
                                                                             tGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGaa                                      >  2:4225562/1‑151 (MQ=255)
                                                                             tGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGaa                                      <  1:2713020/151‑1 (MQ=255)
                                                                               aCGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATc                                    >  1:4249144/1‑151 (MQ=255)
                                                                               aCGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATc                                    >  2:2826286/1‑151 (MQ=255)
                                                                                 gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcac                                  >  2:2358624/1‑151 (MQ=255)
                                                                                  ggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcaca                                 <  2:5571645/151‑1 (MQ=255)
                                                                                   gcatggaTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATcaca                                 <  2:5601121/144‑1 (MQ=255)
                                                                                   gCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAg                                >  2:1617011/1‑151 (MQ=255)
                                                                                     aTCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGtt                              >  2:390378/1‑151 (MQ=255)
                                                                                     aTCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGtt                              >  2:388612/1‑151 (MQ=255)
                                                                                       ggaTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTta                            >  2:186479/3‑151 (MQ=255)
                                                                                       ccATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTta                            <  2:812110/151‑1 (MQ=255)
                                                                                         aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact                          <  1:5960078/151‑1 (MQ=255)
                                                                                         aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact                          >  1:627586/1‑151 (MQ=255)
                                                                                         aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact                          >  2:6825174/1‑151 (MQ=255)
                                                                                          tGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtacta                         >  1:2913333/1‑151 (MQ=255)
                                                                                           gCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtactac                        >  2:1896090/1‑151 (MQ=255)
                                                                                           gCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtactac                        <  1:671995/151‑1 (MQ=255)
                                                                                           gCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCCCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtactac                        <  1:1611035/151‑1 (MQ=255)
                                                                                            cccGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTactact                       <  1:3662464/151‑1 (MQ=255)
                                                                                             ccGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTg                      >  2:3582167/1‑151 (MQ=255)
                                                                                              cGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTgg                     >  1:5231161/1‑151 (MQ=255)
                                                                                               gTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGc                    >  1:5198110/1‑151 (MQ=255)
                                                                                               gTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGc                    <  1:3434058/151‑1 (MQ=255)
                                                                                                  aCCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCt                 >  2:3783007/1‑151 (MQ=255)
                                                                                                  aCCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCt                 >  1:322858/1‑151 (MQ=255)
                                                                                                    ctgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTc               >  2:1872191/4‑151 (MQ=255)
                                                                                                     tgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCa              >  1:1520927/3‑151 (MQ=255)
                                                                                                       cctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagc            <  1:944360/151‑1 (MQ=255)
                                                                                                        ctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagct           <  1:500907/151‑1 (MQ=255)
                                                                                                        ctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagct           >  1:5081319/1‑151 (MQ=255)
                                                                                                        ctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagct           <  2:3131706/151‑1 (MQ=255)
                                                                                                        ctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagct           <  2:6743930/151‑1 (MQ=255)
                                                                                                        ctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagct           >  2:1520837/1‑151 (MQ=255)
                                                                                                         tccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctg          <  1:5429883/151‑1 (MQ=255)
                                                                                                           cGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgag        >  1:4718060/1‑151 (MQ=255)
                                                                                                            gCAAGGCCTGCGTTCGGCCTCATGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagc       <  1:1550590/151‑1 (MQ=255)
                                                                                                             cAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagct      <  2:4838659/151‑1 (MQ=255)
                                                                                                              aaGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagctg     >  1:5358999/1‑151 (MQ=255)
                                                                                                              aaGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagctg     >  1:6476680/1‑151 (MQ=255)
                                                                                                                ggCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTggg   <  1:684486/151‑1 (MQ=255)
                                                                                                                     |                                                                                                                                                  
GCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTGGGG  >  CP009974/4147314‑4147577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑