Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,537,135	C→G	L96L (CTG→CTC)	RPPX_20270 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,537,135	0	C	G	100.0%	128.6 / NA	51	L96L (CTG→CTC)	RPPX_20270	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (28/23);  total (28/23)

GGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGT  >  CP009974/4536987‑4537174                                                                                                                                                     |                                        ggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAg                                       >  2:3815287/1‑151 (MQ=255)  gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAgg                                      >  1:6595821/1‑151 (MQ=255)   cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                                     >  1:1666956/1‑151 (MQ=255)   cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                                     >  1:2535237/1‑151 (MQ=255)   cAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGc                                     <  2:5835032/151‑1 (MQ=255)    aGATGCGATACCAGCAGGGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                                    <  2:3334512/151‑1 (MQ=255)     gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                                   >  1:1773526/1‑151 (MQ=255)     gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCGCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                                   <  2:6783157/151‑1 (MQ=255)      aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                                  <  1:4601881/151‑1 (MQ=255)      aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                                  >  2:1890574/1‑151 (MQ=255)      aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                                  <  1:2480432/151‑1 (MQ=255)       tGCCATACCAGCAGGGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                                 <  2:5427116/151‑1 (MQ=255)       tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                                 <  2:2051185/151‑1 (MQ=255)       tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                                 >  2:104747/1‑151 (MQ=255)       ggCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                                 <  2:366248/150‑1 (MQ=255)        gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                                >  2:3669529/1‑151 (MQ=255)        gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                                <  1:6072107/151‑1 (MQ=255)         ccATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATgg                               <  1:4505097/151‑1 (MQ=255)         ccATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATgg                               >  2:5180755/1‑151 (MQ=255)          cATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGc                              >  1:3251651/1‑151 (MQ=255)           aTACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCg                             >  2:748425/1‑151 (MQ=255)           aTACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCg                             >  2:2841877/1‑151 (MQ=255)           aTACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCg                             >  1:5096498/1‑151 (MQ=255)            tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGCGGCAGATGGCgg                            >  2:6813370/1‑150 (MQ=255)            tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt                            <  1:6998433/151‑1 (MQ=19)            tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt                            >  1:6636090/1‑151 (MQ=25)            tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt                            >  1:7222734/1‑151 (MQ=25)             aCCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTg                           >  1:2395009/1‑151 (MQ=19)             aCCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTg                           >  1:2554400/1‑151 (MQ=18)              ccAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGc                          >  1:3704256/1‑151 (MQ=19)              ccAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCCGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGCGAGGCAGATGGCGTGc                          >  2:3548659/1‑151 (MQ=255)               cggcggagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAGCGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCa                         <  2:1487737/146‑1 (MQ=11)               cagcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCa                         >  1:946722/1‑151 (MQ=19)               cagcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCa                         >  1:6007111/1‑151 (MQ=18)                agcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAg                        <  1:5158771/151‑1 (MQ=16)                 gcggagGGCGACGGTCAGGTGGTGGGGGACCAGCCCGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGt                       <  2:725638/148‑1 (MQ=11)                 gcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGt                       >  2:2849183/1‑151 (MQ=18)                 gcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGt                       <  2:2077246/151‑1 (MQ=9)                  cagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTg                      <  2:3007186/151‑1 (MQ=12)                   agagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTgg                     <  1:5485517/151‑1 (MQ=9)                    gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc                    <  1:2540417/151‑1 (MQ=9)                     agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCt                   >  1:4997154/1‑151 (MQ=14)                     agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCt                   >  2:4945864/1‑151 (MQ=17)                      gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCtt                  >  1:2342099/1‑151 (MQ=9)                      gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCtt                  >  2:5952172/1‑151 (MQ=14)                       ggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTtg                 <  2:6695933/151‑1 (MQ=0)                       ggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTtg                 <  2:6742542/151‑1 (MQ=2)                       ggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTtg                 <  2:5716021/151‑1 (MQ=11)                       ggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTtg                 <  2:5715988/151‑1 (MQ=2)                            cGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGCGGGAGGCAGATGGCGTGCAGTGGCCTGTGcca            >  2:3700105/1‑151 (MQ=255)                                      gtcggggAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGt  <  1:5742847/148‑1 (MQ=2)                                                                                                                                                     |                                        GGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACCAGTTGGGT  >  CP009974/4536987‑4537174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑