BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,540,460	C→T	R458R (CGG→CGA)	RPPX_20280 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,540,460	0	C	T	99.7%	1057.0 / NA	320	R458R (CGG→CGA)	RPPX_20280	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (162/156); total (164/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

GCCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAACGCCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGATG  >  CP009974/4540312‑4540604

gcCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCg                                                                                                                                                >  1:1624603/1‑151 (MQ=255)

        aaTCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGCCGGTCATGAt                                                                                                                                        >  2:4947172/1‑151 (MQ=255)

            ccTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTc                                                                                                                                    <  1:5890288/151‑1 (MQ=255)

             cTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCg                                                                                                                                   >  1:3793547/1‑151 (MQ=255)

              tCGGTCAGGTTGTCGCTGGCGCAGGAGGAGGAGGCGATGGCGTCGGCTTTGTCCTTGCACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGGTGGTCCAGCCATTGATCCAGGCGTTTGAAGCGTCGGTCATGATCTTCgg                                                                                                                                  <  2:148031/151‑1 (MQ=14)

              tCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCgg                                                                                                                                  >  2:1903455/1‑151 (MQ=255)

              tCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCgg                                                                                                                                  >  1:3334624/1‑151 (MQ=255)

               cGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTCTGTCCTTGAACGGGTTGAACGGCGCCACGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCggc                                                                                                                                 >  2:654081/1‑150 (MQ=255)

                  tCACGTTGTCGCTGGCGCCGAACAAGGAGCCGGTGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGcc                                                                                                                              <  2:6501928/151‑1 (MQ=255)

                    aCGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCgg                                                                                                                            <  2:783151/151‑1 (MQ=255)

                       ttGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGgtg                                                                                                                         >  1:3023682/1‑151 (MQ=255)

                       ttGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGgtg                                                                                                                         <  1:811600/151‑1 (MQ=255)

                        tGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCGGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGgtgt                                                                                                                        <  1:3192290/151‑1 (MQ=255)

                            gCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGc                                                                                                                    >  1:1732744/1‑151 (MQ=255)

                              tGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTg                                                                                                                  <  2:6768964/151‑1 (MQ=255)

                               ggCGCCGGACAAGGAGGCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTgg                                                                                                                 <  2:6106346/151‑1 (MQ=255)

                               ggCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTgg                                                                                                                 >  1:82963/1‑151 (MQ=255)

                                  gcCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGccc                                                                                                              >  1:2687075/1‑151 (MQ=255)

                                   ccGACCAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCa                                                                                                             <  1:3453081/151‑1 (MQ=255)

                                   ccGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCa                                                                                                             >  2:6595342/1‑151 (MQ=255)

                                    cGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCAt                                                                                                            <  1:6990455/151‑1 (MQ=255)

                                    cGAACAAGGAGCCGATGGCGTCGGCTTGGTCCTGCAACGTGATAAGCGGCGCGAACGGGGGGCGAAGCGGGTGGCCGGGCTGGGCCCGTCATTGCGCCAGGCGGGTGAAGCGGGGCTCAAGCGGTTCGGCGGCGGGTGTCGACAGGcgcta                                                                                                            >  2:6290513/1‑147 (MQ=255)

                                     gAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCAtt                                                                                                           <  2:2198970/151‑1 (MQ=255)

                                         aaGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGt                                                                                                       <  2:3865669/151‑1 (MQ=255)

                                            gAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGaa                                                                                                    <  2:4847650/151‑1 (MQ=255)

                                            gAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGaa                                                                                                    <  2:6697310/151‑1 (MQ=255)

                                            gAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGaa                                                                                                    <  1:191343/151‑1 (MQ=255)

                                               ccGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCa                                                                                                 <  2:635307/151‑1 (MQ=255)

                                                 gATGGCGTCGGCTTTGTGCTTGAACGGGTTGAGCGGCGCCAAGGCGGTGTAAGGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGc                                                                                               <  2:5362313/151‑1 (MQ=11)

                                                 gATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTGATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGc                                                                                               >  2:4476274/1‑151 (MQ=255)

                                                    ggCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAAc                                                                                            <  2:11521/151‑1 (MQ=255)

                                                     gCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCCTTGGGTGAATCAGCAACg                                                                                           >  2:1430036/1‑151 (MQ=16)

                                                     gCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACg                                                                                           >  1:6818114/1‑151 (MQ=21)

                                                      cGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGc                                                                                          >  1:2136521/1‑151 (MQ=18)

                                                        tCGACTGTGTCCATGAACGGGTTTAACGACGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTAAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCa                                                                                        <  2:6979496/151‑1 (MQ=11)

                                                         cGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAg                                                                                       <  1:3651705/151‑1 (MQ=17)

                                                         cGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAg                                                                                       <  2:4695509/151‑1 (MQ=12)

                                                           gCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTg                                                                                     >  1:5677179/1‑151 (MQ=17)

                                                             tttGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTgag                                                                                   <  1:6116299/151‑1 (MQ=12)

                                                             tttGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTgag                                                                                   >  1:3829916/1‑151 (MQ=12)

                                                              ttGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTgaga                                                                                  <  1:6022513/151‑1 (MQ=17)

                                                                  ccTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTc                                                                              >  1:2229409/1‑151 (MQ=9)

                                                                    ttGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCa                                                                            >  2:2537687/1‑151 (MQ=14)

                                                                      gAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGa                                                                          <  1:621743/151‑1 (MQ=12)

                                                                       aaCGGGTTGAACGGCGCGAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGAc                                                                         <  2:7154261/151‑1 (MQ=12)

                                                                         ggggTTGAACGGGGCCAAGGGGGTGTAGAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcg                                                                       <  2:3607467/150‑1 (MQ=11)

                                                                         cGGGTTGAGCGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcg                                                                       <  2:2961423/151‑1 (MQ=255)

                                                                         cGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcg                                                                       <  1:3594580/151‑1 (MQ=12)

                                                                          gggTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcgc                                                                      >  1:7166644/1‑151 (MQ=16)

                                                                          gggTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcgc                                                                      <  1:6302783/151‑1 (MQ=18)

                                                                           ggTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcgcg                                                                     >  2:2293157/1‑151 (MQ=16)

                                                                            gTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgg                                                                    >  2:1654127/1‑151 (MQ=18)

                                                                             ttGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCCGTGAGATTTCCAGACCgcggg                                                                   >  2:5751972/1‑151 (MQ=255)

                                                                               gAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgggcg                                                                 >  1:4669255/1‑151 (MQ=255)

                                                                                aaCGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgggcgg                                                                <  2:5990337/151‑1 (MQ=255)

                                                                                 aCGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGa                                                               <  2:366118/151‑1 (MQ=255)

                                                                                  cGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAg                                                              >  1:5061285/1‑151 (MQ=25)

                                                                                   ggCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGGATCAGCAACGCCAGTGAGATGTCCAGACCGCGGGCGGAgg                                                             >  2:180209/1‑151 (MQ=255)

                                                                                   ggCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAgg                                                             >  1:375797/1‑151 (MQ=255)

                                                                                   ggCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAgg                                                             >  2:2013180/1‑151 (MQ=255)

                                                                                    gcgcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGt                                                            <  1:3942524/151‑1 (MQ=21)

                                                                                      gcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGc                                                          <  1:4183904/151‑1 (MQ=255)

                                                                                      gcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGc                                                          >  2:3529656/1‑151 (MQ=19)

                                                                                      gcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGc                                                          <  2:586036/151‑1 (MQ=25)

                                                                                          aGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTc                                                      <  2:5214638/151‑1 (MQ=255)

                                                                                          aGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTc                                                      <  2:4677363/151‑1 (MQ=18)

                                                                                            gCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGt                                                    <  1:4837123/151‑1 (MQ=255)

                                                                                               gtgtAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCAc                                                 <  1:5796851/151‑1 (MQ=255)

                                                                                                      gCGGGCTTCCGTGCTGGCCCGGCCATTGCTCCGGGCGTGTGAAGCGTCGGTCATGCGCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTa                                          <  2:2365361/151‑1 (MQ=11)

                                                                                                         ggCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACa                                       <  1:4594571/151‑1 (MQ=255)

                                                                                                         gcgtgtgGTGCTGGTCCAGCGATTGCGCCGGGGGTTTGAGGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCGATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACa                                       <  2:2458330/148‑1 (MQ=11)

                                                                                                           cTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAgg                                     >  1:6685481/1‑151 (MQ=255)

                                                                                                              tCGTGCTGGCCGAGCCATTGCTCCAGGCGTTGGAAGCCTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGACCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGGGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCa                                  <  2:5685970/151‑1 (MQ=11)

                                                                                                                gTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAg                                >  1:1205798/1‑151 (MQ=255)

                                                                                                                gTGCAGGGCCAGCCGTTAGTCCGGTCGTCTGAGGCGTCCGTCCTGCTCTGCGGTCCCGGGCGTCGTCGGACCCATAGGGCCACTCAGGAACCCCAGTGAGATTTCCAGACCCCGGGCGGAAGTGCGTTCGTCACGGTCGTAACAGGCTAAg                                <  2:6153564/151‑1 (MQ=255)

                                                                                                                 tGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAgg                               >  2:2594112/1‑151 (MQ=255)

                                                                                                                  gCTGGTCCAGCCATTGCTCCAGGCGTTTGAGGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAAGCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAggc                              >  2:4337530/1‑151 (MQ=255)

                                                                                                                  gCTGGTCCAGCCAGTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAggc                              <  2:893232/151‑1 (MQ=255)

                                                                                                                     ggTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAggcggc                           <  2:2686710/151‑1 (MQ=255)

                                                                                                                       tCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTg                         <  1:2810146/151‑1 (MQ=255)

                                                                                                                       tCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCACTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTg                         <  1:1076880/151‑1 (MQ=255)

                                                                                                                        ccAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGc                        <  1:6727068/151‑1 (MQ=255)

                                                                                                                        ccAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGc                        <  1:6042355/151‑1 (MQ=255)

                                                                                                                         cAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGcc                       <  1:6534114/151‑1 (MQ=255)

                                                                                                                            ccATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAgg                    <  2:6876361/151‑1 (MQ=255)

                                                                                                                            ccATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCATTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAgg                    >  1:2571576/1‑151 (MQ=255)

                                                                                                                             cATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGc                   >  1:4501459/1‑151 (MQ=255)

                                                                                                                              aTTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCt                  >  2:3824335/1‑151 (MQ=255)

                                                                                                                              aTTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCt                  >  1:4034428/1‑151 (MQ=255)

                                                                                                                               ttGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTg                 <  1:1235288/151‑1 (MQ=255)

                                                                                                                                tGCTCCAGGCGTTTGAAGCGTCGGTCATGATCGTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGt                <  1:3692928/151‑1 (MQ=255)

                                                                                                                                ggCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGCTTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGt                <  2:5154868/150‑1 (MQ=255)

                                                                                                                                 gCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTgga               >  2:3978751/1‑149 (MQ=255)

                                                                                                                                   tCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCCGGCTGTAgg             >  2:5509711/1‑151 (MQ=255)

                                                                                                                                   tCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAgg             <  1:2359684/151‑1 (MQ=255)

                                                                                                                                    ccAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAggg            <  1:7199340/151‑1 (MQ=255)

                                                                                                                                     cAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAgggg           <  1:4777447/151‑1 (MQ=255)

                                                                                                                                      aGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGt          >  2:5088702/1‑151 (MQ=255)

                                                                                                                                             tGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTTACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGAt   >  1:931552/1‑151 (MQ=255)

                                                                                                                                             tGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGAt   <  1:5710961/151‑1 (MQ=255)

GCCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAACGCCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGATG  >  CP009974/4540312‑4540604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`