Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,541,504 C→T L110L (CTG→CTA)  RPPX_20280 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,541,5040CT99.7% 1161.7 / NA 339L110L (CTG→CTA) RPPX_20280hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (166/172);  total (167/172)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 339 total aligned reads displayed.

GGCGCATGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGACAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCCGGGCGCAAAGCACGG  >  CP009974/4541356‑4541651
                                                                                                                                                    |                                                                                                                                                   
 gggcAGGTGCGGTGTGCGCAGGGCTGCCGAACTGGTGATCCGGGCGGTGGTGGCGTTGGGCCACAGGTGCGGGCTGTCGCCGATCCACACTGCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAgg                                                                                                                                                  <  2:243890/149‑1 (MQ=11)
     aggtgCCGTTTGGGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAgc                                                                                                                                              <  2:5839931/149‑1 (MQ=255)
     aTGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAgc                                                                                                                                              >  1:7091995/1‑151 (MQ=38)
      tgtgCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAgcg                                                                                                                                             <  1:2947944/151‑1 (MQ=255)
      tgtgCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAgcg                                                                                                                                             <  1:4471454/151‑1 (MQ=255)
       gtgCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAgcgc                                                                                                                                            <  2:1959339/151‑1 (MQ=255)
         gCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTc                                                                                                                                          >  1:156194/1‑151 (MQ=38)
            gTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCAcg                                                                                                                                       <  2:2947894/151‑1 (MQ=255)
            gTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCAcg                                                                                                                                       <  2:577169/151‑1 (MQ=255)
              ttGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCAcggc                                                                                                                                     >  1:6295907/1‑151 (MQ=38)
                 ggcAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCggt                                                                                                                                  <  1:5917322/150‑1 (MQ=255)
                   cAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCggtgg                                                                                                                                <  1:1874067/151‑1 (MQ=37)
                   cAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCggtgg                                                                                                                                >  2:5377632/1‑151 (MQ=38)
                    ggggCGGCGGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACGGGGGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCggtggt                                                                                                                               <  2:4350665/150‑1 (MQ=11)
                       gCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGt                                                                                                                            <  2:3116084/151‑1 (MQ=255)
                         tGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACCCTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGgcc                                                                                                                          >  2:4089172/1‑151 (MQ=255)
                           ccGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGtcctc                                                                                                                        <  1:867299/151‑1 (MQ=255)
                             gAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCa                                                                                                                      <  2:2935165/151‑1 (MQ=38)
                               aCCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGc                                                                                                                    >  2:1681118/1‑151 (MQ=38)
                                ccGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGTCAGGTGGCTGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCGCGGCGGGGGTAGTCCTCCAGcc                                                                                                                   >  2:2725702/1‑151 (MQ=11)
                                  ggTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCt                                                                                                                 <  1:6193088/151‑1 (MQ=37)
                                  ggTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACGTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGGCGCGCTCACGGCGGGGGTAGCCCTCCAGcccc                                                                                                                 >  2:4202465/1‑150 (MQ=11)
                                    tGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTg                                                                                                               >  1:1905534/1‑151 (MQ=255)
                                     gATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTgg                                                                                                              <  1:4087909/151‑1 (MQ=255)
                                     gATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTgg                                                                                                              >  1:2773841/1‑151 (MQ=255)
                                     gATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTgg                                                                                                              >  1:4649445/1‑151 (MQ=255)
                                     gATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTgg                                                                                                              >  1:5748783/1‑151 (MQ=255)
                                      aTACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGt                                                                                                             >  1:3265476/1‑151 (MQ=255)
                                      aTACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGt                                                                                                             >  1:6489828/1‑151 (MQ=255)
                                      aTACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGt                                                                                                             <  1:3239532/151‑1 (MQ=255)
                                       tACGGGCGGTGATGGCGTTGGTCCACGGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTa                                                                                                            <  2:7006005/151‑1 (MQ=255)
                                       tACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTa                                                                                                            >  2:1011391/1‑151 (MQ=255)
                                          gggCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagct                                                                                                         <  2:3965151/151‑1 (MQ=255)
                                          gggCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagct                                                                                                         <  2:3711673/151‑1 (MQ=255)
                                           ggCGGTGATGGCGTTGGTCCACGGGTGCGGGCTGTGGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctc                                                                                                        <  2:5191050/151‑1 (MQ=255)
                                           ggCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctc                                                                                                        >  1:5690846/1‑151 (MQ=255)
                                           ggCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctc                                                                                                        <  2:1468524/151‑1 (MQ=255)
                                            gCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctca                                                                                                       <  1:2695933/151‑1 (MQ=255)
                                             cGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagcccag                                                                                                      >  2:717321/1‑151 (MQ=255)
                                              ggTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctcagc                                                                                                     <  1:6066280/151‑1 (MQ=255)
                                                tGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTagctcagctc                                                                                                   >  1:4352090/1‑151 (MQ=255)
                                                   tgttgTTGGTCCACGGGGGCGCGCTCTCGCCGATCCACACTTCCTTGGCGGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGGCCTTGGTAGCTCAGCTCttt                                                                                                <  2:638002/147‑1 (MQ=11)
                                                   tGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCttt                                                                                                <  1:478380/151‑1 (MQ=255)
                                                    ggCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTg                                                                                               >  2:1590411/1‑151 (MQ=255)
                                                      cGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTa                                                                                             >  2:2153016/1‑151 (MQ=255)
                                                       gTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTag                                                                                            >  1:2195965/1‑151 (MQ=255)
                                                         tGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTagag                                                                                          >  1:2620086/1‑151 (MQ=255)
                                                          ggTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAgg                                                                                         >  1:3902708/1‑151 (MQ=255)
                                                           gTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGCGGc                                                                                        >  2:3435949/1‑151 (MQ=255)
                                                           gTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGc                                                                                        >  1:5387706/1‑151 (MQ=255)
                                                            tCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGcc                                                                                       >  2:5323631/1‑151 (MQ=255)
                                                             ccACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGccc                                                                                      <  1:115279/151‑1 (MQ=255)
                                                              cacaGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCcg                                                                                     <  1:790795/151‑1 (MQ=255)
                                                               acaGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCcgc                                                                                    >  2:1758248/1‑151 (MQ=255)
                                                                    tGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGa                                                                               <  2:6489828/151‑1 (MQ=255)
                                                                     gCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGAc                                                                              >  2:1347876/1‑151 (MQ=255)
                                                                     gCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGAc                                                                              <  1:3801589/151‑1 (MQ=255)
                                                                       gggCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGt                                                                            <  2:447454/151‑1 (MQ=255)
                                                                         gCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGc                                                                          >  1:1438510/1‑151 (MQ=255)
                                                                         gCTGTAGCCGATCCACACTTCCTTCGCCGTGTCCTGGCTGGTGTCGGCCCACACCCAGCAGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGCAGTCCTCCAGTCCTTTGTAGCTCAGCTCTGTATAGCAGCCCGCGCCGAGGttc                                                                          >  2:1039326/1‑149 (MQ=14)
                                                                           tGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTc                                                                        >  1:1012439/1‑151 (MQ=255)
                                                                           tGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTc                                                                        <  1:1702155/151‑1 (MQ=255)
                                                                           tGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTc                                                                        <  2:2807314/151‑1 (MQ=255)
                                                                            gTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCGGGATAGGTAGCGCTTACGGCGGTGGTGGTCCTCCAGCCCTTGGGAGCTCAGCTCGTTGGAGAGGCCGGCGCAGGCGttccga                                                                       >  2:866864/1‑146 (MQ=14)
                                                                              aGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCatg                                                                     <  1:3014079/151‑1 (MQ=255)
                                                                              aGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCatg                                                                     >  1:4166380/1‑151 (MQ=255)
                                                                               gCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACCTGCTCatga                                                                    >  1:5248868/1‑151 (MQ=255)
                                                                                ccGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCatgaa                                                                   >  1:7202182/1‑151 (MQ=255)
                                                                                 cGATCCACACTTCCGTGGCCGTGTCCTGGCAGGTGTCGGCCCACGCCCGGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGGAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCatgaat                                                                  <  2:4997134/151‑1 (MQ=255)
                                                                                   aTCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCatgaatga                                                                <  1:6395433/151‑1 (MQ=255)
                                                                                    tCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCAtgactgac                                                               >  1:586817/1‑151 (MQ=255)
                                                                                      cacaCTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCa                                                             <  2:5407366/151‑1 (MQ=255)
                                                                                       acacTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAg                                                            >  2:4884838/1‑151 (MQ=255)
                                                                                        cacTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGc                                                           >  2:6420161/1‑151 (MQ=255)
                                                                                         ccTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCt                                                          <  1:6917108/150‑1 (MQ=255)
                                                                                            gccTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCtttt                                                       <  2:627984/150‑1 (MQ=255)
                                                                                             ccTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTc                                                      >  1:3624605/1‑151 (MQ=255)
                                                                                             ccTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTc                                                      <  1:3581024/151‑1 (MQ=255)
                                                                                              cTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCg                                                     <  2:5024841/151‑1 (MQ=255)
                                                                                                tGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGcc                                                   <  2:734232/151‑1 (MQ=255)
                                                                                                      tgtCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAgg                                             >  2:6991904/1‑151 (MQ=21)
                                                                                                         ccTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGcc                                          >  1:1317115/1‑151 (MQ=25)
                                                                                                            ggCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCttt                                       <  2:3819137/151‑1 (MQ=25)
                                                                                                                ggTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATg                                   >  1:195634/1‑151 (MQ=25)
                                                                                                                ggTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATg                                   <  1:5947122/151‑1 (MQ=25)
                                                                                                                       gCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGt                            >  2:4753552/1‑151 (MQ=25)
                                                                                                                       gCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGt                            >  1:3493174/1‑151 (MQ=25)
                                                                                                                       gCCCACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGt                            >  1:196353/1‑151 (MQ=21)
                                                                                                                        cccACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTa                           <  1:4248296/151‑1 (MQ=21)
                                                                                                                        cccACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTa                           >  2:3389246/1‑151 (MQ=21)
                                                                                                                        cccACACCCAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTa                           <  2:2555383/151‑1 (MQ=21)
                                                                                                                               ccAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAg                    >  1:5077887/1‑151 (MQ=25)
                                                                                                                               ccAGCCGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGGGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAg                    >  2:2287759/1‑151 (MQ=21)
                                                                                                                                   ccGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGcctg                <  2:2401267/151‑3 (MQ=21)
                                                                                                                                       tGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgcg            <  1:3602945/151‑1 (MQ=255)
                                                                                                                                       tGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGgcg            <  2:5628477/151‑1 (MQ=25)
                                                                                                                                          tctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaa         >  2:6729898/1‑151 (MQ=255)
                                                                                                                                           ctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaaa        <  1:6660860/151‑1 (MQ=18)
                                                                                                                                              gCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcg     >  2:1150333/1‑150 (MQ=19)
                                                                                                                                                cGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcgcg   <  2:1116855/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGCATGTGCCGTTTGCGCAGGGCTGCCGAACCGGTGATACGGGCGGTGATGGCGTTGGTCCACAGGTGCGGGCTGTAGCCGATCCACACTTCCTTGGCCGTGTCCTGGCAGGTGTCGGCCCACACCCAGCCGGTGCTCTGGCCGGACAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCCGGGCGCAAAGCACGG  >  CP009974/4541356‑4541651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑