BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,543,776	C→G	K449N (AAG→AAC)	RPPX_20290 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,543,776	0	C	G	98.9%	466.9 / ‑6.1	177	K449N (AAG→AAC)	RPPX_20290	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (80/95); minor base T (2/0); total (82/95)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TGGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAAGCCGTCTTGAGCGATAGGATGTTGAACCACAGCATCCCCGCGTTTAAACCACCACCACCGCCCGAGGTGAGCAACCCAAGGAAGTTGGGT  >  CP009974/4543628‑4543860

gggCACTTAATGTCAGCATGACGGCGTTATGCGGTGCCCGCACGCTGACCAGTGGCGCTGCCGCTGCGCCTATTCCCCCGAACACAGATGAAGCGAAACCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGtt                                                                                    <  2:5259586/150‑1 (MQ=14)

  gCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGa                                                                                  >  2:3398270/1‑151 (MQ=255)

  gCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGa                                                                                  >  1:600856/1‑151 (MQ=255)

  gCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGCCGCCCTTCTGCAGGCTGTAGTAGGCCGTGTTgc                                                                                  >  2:4992405/1‑150 (MQ=17)

   cACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAg                                                                                 >  1:5527733/1‑151 (MQ=255)

    aCTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGc                                                                                <  1:5071035/151‑1 (MQ=255)

       tAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGAt                                                                             <  1:3487734/151‑1 (MQ=255)

       tAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGAt                                                                             >  1:905001/1‑151 (MQ=255)

        aaTCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATa                                                                            <  1:2637413/151‑1 (MQ=255)

         aTCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATaa                                                                           >  1:4407100/1‑150 (MQ=255)

         aTCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATaa                                                                           >  1:4646116/1‑150 (MQ=255)

          tctcAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATaag                                                                          >  2:1026213/1‑149 (MQ=255)

          cctcAGCATGACGGCGTTATTCGTTGCCCGCACGCTGACCAATGCCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATaag                                                                          <  2:7166976/150‑3 (MQ=14)

           ctcAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGa                                                                         >  1:5350950/1‑151 (MQ=255)

            tcAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGAt                                                                        <  1:1972775/151‑1 (MQ=255)

             cAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATg                                                                       >  2:4902717/1‑151 (MQ=255)

              aGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGt                                                                      >  2:545154/1‑151 (MQ=255)

              aGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGt                                                                      <  2:2159529/151‑1 (MQ=255)

               gCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGtt                                                                     <  2:1523771/151‑1 (MQ=255)

                 ttGACGGCCTTATGCGTTGCCCGCACGCTGACTAATGTCGCTGCCGCTGCGCCAATCACCCCGAAGACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTGGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGa                                                                   <  2:747192/150‑1 (MQ=11)

                   gACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAAc                                                                 <  1:6719852/151‑1 (MQ=255)

                   gACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAAc                                                                 >  1:4460074/1‑151 (MQ=255)

                   gACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGACGCAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGGTTTGAGCGACAAGATGTTGAAc                                                                 >  1:3258792/1‑151 (MQ=14)

                     cGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACca                                                               <  2:4394954/151‑1 (MQ=255)

                     cGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACca                                                               <  1:801229/151‑1 (MQ=255)

                      ggCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACTCAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcac                                                              <  1:5283648/151‑1 (MQ=255)

                       gCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcaca                                                             <  1:914322/151‑1 (MQ=255)

                       gCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcaca                                                             >  2:5129117/1‑151 (MQ=255)

                       gCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcaca                                                             <  1:3813645/151‑1 (MQ=255)

                       gCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACcaca                                                             <  2:4964618/151‑1 (MQ=255)

                       gCCTGATGCGTTTCCCGCACGCTGACCAATCTCGCTGCCGCGGCGCCCATCACTCCGAACACAGCTTAAGCGAACCCCAGCGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTCTAGGCCGTGTTGAGCCATCAGATTTTCAAccccc                                                             >  2:1609285/1‑148 (MQ=14)

                        ccTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAg                                                            >  1:1037055/1‑151 (MQ=255)

                           tATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCAt                                                         <  2:4758314/151‑1 (MQ=255)

                            aTGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATc                                                        >  1:6157162/1‑151 (MQ=255)

                             tGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATcc                                                       <  2:5043700/151‑1 (MQ=255)

                               cGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATccct                                                     <  2:575361/151‑2 (MQ=255)

                                gTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATccctg                                                    >  1:1549180/1‑149 (MQ=255)

                                gTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATccctg                                                    >  1:5753883/1‑149 (MQ=255)

                                 ttGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATccctgc                                                   >  1:7169024/1‑148 (MQ=255)

                                 ttGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCtgc                                                   >  1:7168799/1‑151 (MQ=255)

                                   gCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGt                                                 <  2:5981284/151‑1 (MQ=255)

                                    cccGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGtt                                                <  2:2852822/151‑1 (MQ=11)

                                      cGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTa                                              <  1:3545702/151‑1 (MQ=11)

                                      cGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTa                                              >  2:3925045/1‑151 (MQ=255)

                                        cACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTaaa                                            <  1:1564269/151‑1 (MQ=11)

                                         aCGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAac                                           <  1:2804356/151‑1 (MQ=11)

                                         aCGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAac                                           <  1:1217828/151‑1 (MQ=255)

                                          cGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAacc                                          <  1:1758463/151‑1 (MQ=255)

                                             tGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCTAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccacc                                       <  1:4268888/151‑1 (MQ=11)

                                             tGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccacc                                       <  2:3554625/151‑1 (MQ=11)

                                              gACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccc                                      >  2:5198662/1‑150 (MQ=11)

                                               aCCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccac                                     <  1:855764/151‑1 (MQ=11)

                                                ccAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccacc                                    <  2:2264227/151‑1 (MQ=11)

                                                ccAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccacc                                    <  2:1344824/151‑1 (MQ=11)

                                                 cAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccacca                                   <  2:1369940/151‑1 (MQ=11)

                                                  aaTGTCGCTGCCGCTGCGCCAATCGCCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccaccac                                  <  2:4092021/151‑1 (MQ=11)

                                                   aTGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAaccaccaccacc                                 <  2:4494113/151‑1 (MQ=11)

                                                    tGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGTATTCTTTCGTTGATACCGCCACCACCg                                <  2:3659295/151‑1 (MQ=11)

                                                    tGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGCTTAAaccaccaccaccc                                >  2:4589915/1‑150 (MQ=11)

                                                     gTCGCTGCCGCTGCGCCAATCACCCCGGACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGc                               <  2:5883965/151‑1 (MQ=11)

                                                     gTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGc                               >  1:1641032/1‑151 (MQ=255)

                                                      tCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGcc                              <  2:6514791/151‑1 (MQ=255)

                                                       cgctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGccg                             <  2:1918648/151‑2 (MQ=11)

                                                       cgctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGccg                             >  2:142586/1‑150 (MQ=255)

                                                       cgctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGccg                             <  2:5141059/151‑2 (MQ=11)

                                                       cgctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGccg                             >  2:3561732/1‑150 (MQ=255)

                                                         ctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGa                           <  2:825808/151‑1 (MQ=11)

                                                         ctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGa                           <  2:6732785/151‑1 (MQ=11)

                                                           gccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAgg                         >  1:7136291/1‑151 (MQ=11)

                                                           gccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAgg                         >  1:2935972/1‑151 (MQ=11)

                                                           gccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAgg                         >  1:3176239/1‑151 (MQ=11)

                                                           gccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCCTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTCAACCACAGCATCCCTGCTGTTAAACCACCACCcccgcccgccc                         >  2:6876058/1‑148 (MQ=11)

                                                            ccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGt                        <  2:4259689/151‑1 (MQ=11)

                                                             cgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTg                       >  2:5281516/1‑151 (MQ=11)

                                                              gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                      >  2:2008467/1‑151 (MQ=11)

                                                              gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                      >  2:4760152/1‑151 (MQ=11)

                                                              gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                      <  1:2144022/151‑1 (MQ=11)

                                                               ctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAg                     <  2:2940390/151‑1 (MQ=11)

                                                                tgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGc                    <  2:7008495/151‑1 (MQ=11)

                                                                tgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGc                    <  1:2795194/151‑1 (MQ=11)

                                                                 gcgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCa                   >  1:3025397/1‑151 (MQ=11)

                                                                  cgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGCGGTgcgcca                  >  2:4480950/1‑149 (MQ=11)

                                                                     cAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTTTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTTCGTTTAAACCACCACCACCGCCGGAGGTGAGCAAccc               >  1:2156531/1‑151 (MQ=11)

                                                                     cAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAAccc               <  2:5606110/151‑1 (MQ=11)

                                                                      aaTCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCa              >  1:6297041/1‑151 (MQ=11)

                                                                       aTCACCCCGAACCCAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCaa             <  1:4048149/151‑1 (MQ=11)

                                                                         cACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCCACCCACgg           >  2:6625083/1‑151 (MQ=11)

                                                                          aCCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGa          >  1:3705675/1‑151 (MQ=11)

                                                                          aCCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGa          >  1:1031700/1‑151 (MQ=11)

                                                                          aCCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGa          >  1:4151851/1‑151 (MQ=11)

                                                                           ccccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGaa         <  2:5987872/151‑1 (MQ=11)

                                                                           ccccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGaa         <  1:4835524/151‑1 (MQ=11)

                                                                              cGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGtt      <  1:2456981/151‑1 (MQ=11)

                                                                              cGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGtt      <  2:5894686/151‑1 (MQ=11)

                                                                              cGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGtt      <  1:5184975/151‑1 (MQ=11)

                                                                               gAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTg     >  2:7072024/1‑151 (MQ=11)

                                                                               gAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTg     <  2:2234256/151‑1 (MQ=11)

                                                                                aaCACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTgg    <  1:4810084/151‑1 (MQ=11)

                                                                                  cacaGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTGGGt  >  1:3698402/1‑151 (MQ=11)

                                                                                  cacaGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTGGGt  >  1:109779/1‑151 (MQ=11)

TGGCACTTAATCTCAGCATGACGGCCTTATGCGTTGCCCGCACGCTGACCAATGTCGCTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAAGCCGTCTTGAGCGATAGGATGTTGAACCACAGCATCCCCGCGTTTAAACCACCACCACCGCCCGAGGTGAGCAACCCAAGGAAGTTGGGT  >  CP009974/4543628‑4543860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`