Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,544,682 T→G S147S (TCA→TCC)  RPPX_20290 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,544,6820TG99.0% 662.9 / NA 207S147S (TCA→TCC) RPPX_20290hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (89/116);  total (91/116)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 208 total aligned reads displayed.

TGCAAGGCCAGGTTGCGCAGGGCGGCAATCCGCCGTTCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGG  >  CP009974/4544536‑4544829
                                                                                                                                                  |                                                                                                                                                   
                                                                  cGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgg                                                                               <  1:2917497/151‑1 (MQ=14)
                                                                  cGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgg                                                                               >  2:1629760/1‑151 (MQ=9)
                                                                   ggTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggg                                                                              <  1:4803661/151‑1 (MQ=9)
                                                                     tATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCTTGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGAGAGGGCAGGCTCCCTGCGTGACGTGCCCAGCCTGACCGCGGCCAGTTCCGGTGAgtggg                                                                            >  2:3503490/1‑151 (MQ=11)
                                                                     tATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGTGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggggg                                                                            >  1:1729711/1‑151 (MQ=16)
                                                                     tATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggggg                                                                            >  1:3141034/1‑151 (MQ=16)
                                                                      attgggcGACATCGAGGCGGTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGc                                                                           <  1:5019250/145‑1 (MQ=11)
                                                                      aTTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGc                                                                           <  1:6711971/151‑1 (MQ=16)
                                                                         tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATc                                                                        <  1:2767612/151‑1 (MQ=255)
                                                                         tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATc                                                                        <  2:1329840/151‑1 (MQ=255)
                                                                         tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGGGAGGGCCTGGAAATGGGACTCGGCCGGCGCCAGGGCAGGCGCCCTGCGTGAAGTGCCCCGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCacc                                                                        >  2:4969793/1‑149 (MQ=11)
                                                                          ggcGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  2:5655491/149‑1 (MQ=16)
                                                                          gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  1:298274/151‑1 (MQ=255)
                                                                          gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  1:3616540/151‑1 (MQ=255)
                                                                           tCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccg                                                                      >  1:4718739/1‑151 (MQ=21)
                                                                              aCATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgccg                                                                   >  2:347616/1‑151 (MQ=16)
                                                                               cATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCgg                                                                  >  2:1062769/1‑151 (MQ=255)
                                                                                aTCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCggg                                                                 >  1:1761673/1‑151 (MQ=19)
                                                                                  ggAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                               <  2:2692680/150‑1 (MQ=255)
                                                                                  cGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                               >  2:5330211/1‑151 (MQ=19)
                                                                                   gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                              <  2:1428180/151‑1 (MQ=25)
                                                                                   gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                              <  2:4498295/151‑1 (MQ=255)
                                                                                   gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                              >  1:1004194/1‑151 (MQ=255)
                                                                                      gcgcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAgg                                                           >  1:4244668/1‑151 (MQ=255)
                                                                                       cgcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGc                                                          >  1:1665914/1‑151 (MQ=255)
                                                                                       cgcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGGGAGGGGGCATCCGCCGGGTACAGGc                                                          >  1:4916373/1‑151 (MQ=255)
                                                                                        gcggggCCAGGGGCGCGCCGGTGGGGGAGTAATCTTCGTGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGct                                                         <  2:7199635/146‑1 (MQ=11)
                                                                                        gcTGTGCGAAGCGGGCGCCTGTGGGGGAGTAGTGTTCATGGGTGAGGGCCTGGAAGTGGGTCGCGGCCGGCGGCAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGct                                                         <  1:6291877/151‑1 (MQ=11)
                                                                                         cTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctc                                                        <  1:2610897/151‑1 (MQ=255)
                                                                                          tgtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctct                                                       >  2:5733572/1‑151 (MQ=255)
                                                                                          tgtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctct                                                       >  1:1403202/1‑151 (MQ=255)
                                                                                          tgtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGCCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGCGGCATCCGCCGCGTACAGGctct                                                       >  2:5493479/1‑151 (MQ=11)
                                                                                           gtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCtctc                                                      >  2:5887921/1‑151 (MQ=255)
                                                                                           gtgCCAGGCGCGCGCCATTGGGGGAGTAAGCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCtctc                                                      <  1:2019606/151‑1 (MQ=255)
                                                                                            tgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCCGTGAGGGGGCATCCGCCGGGTACAGGCTCTcc                                                     >  2:6849606/1‑151 (MQ=255)
                                                                                              ccAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAg                                                   >  1:4563927/1‑151 (MQ=255)
                                                                                               cAGGCGCGCGCCATTGGGGGAGTAGTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGc                                                  <  1:392190/151‑1 (MQ=255)
                                                                                                aGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCcgcg                                                 >  2:4405463/1‑151 (MQ=255)
                                                                                                 ggCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGt                                                <  1:3315351/151‑1 (MQ=255)
                                                                                                 ggCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGt                                                >  1:5282704/1‑151 (MQ=255)
                                                                                                  gcgcgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTc                                               <  1:7064264/151‑1 (MQ=255)
                                                                                                     cgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTg                                            >  2:59817/1‑151 (MQ=255)
                                                                                                      gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTCAAGTGCCCAGGCGGAACGCGGCCCGTTCCGCCGCGGGTGCAGCCGCCGGGTCCAGGCCCTCCGGcccccggg                                           >  2:1829937/1‑144 (MQ=11)
                                                                                                      gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                           <  2:1205964/151‑1 (MQ=255)
                                                                                                      gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                           >  1:2272731/1‑151 (MQ=255)
                                                                                                       gggcATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGGCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGtt                                          <  2:5132864/148‑1 (MQ=255)
                                                                                                       cgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGtt                                          <  2:2277926/151‑1 (MQ=255)
                                                                                                        gcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTc                                         <  1:2699975/151‑1 (MQ=255)
                                                                                                         ccATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTca                                        <  2:5077095/151‑1 (MQ=255)
                                                                                                         ccATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTca                                        >  1:2136591/1‑151 (MQ=255)
                                                                                                           aTTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagc                                      <  2:3635915/151‑1 (MQ=255)
                                                                                                           aTTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagc                                      >  1:6257100/1‑151 (MQ=255)
                                                                                                           aTTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagc                                      <  1:6119628/151‑1 (MQ=255)
                                                                                                             tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCAGGCCCCCTGCTTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAGGGTCCATCCGCCTGGTACAGGCTCTCCAGCGGCTTGTTcagccg                                    >  2:1562174/1‑149 (MQ=11)
                                                                                                             tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                    <  1:6434390/151‑1 (MQ=255)
                                                                                                             tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                    <  1:818381/151‑1 (MQ=255)
                                                                                                             tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                    <  1:4013865/151‑1 (MQ=255)
                                                                                                              gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCagcagc                                   <  2:5530585/151‑1 (MQ=255)
                                                                                                              gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCagcagc                                   >  1:7036278/1‑151 (MQ=255)
                                                                                                               ggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAgcagca                                  <  2:7146161/151‑1 (MQ=255)
                                                                                                               ggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAgcagca                                  <  1:2420912/151‑1 (MQ=255)
                                                                                                                 ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                                >  2:825199/1‑151 (MQ=255)
                                                                                                                 ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                                <  2:4033539/151‑1 (MQ=255)
                                                                                                                 ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                                >  2:759579/1‑151 (MQ=255)
                                                                                                                  gAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATgg                               <  1:6966755/151‑1 (MQ=255)
                                                                                                                   ggTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGc                              <  1:1501619/150‑1 (MQ=255)
                                                                                                                   aGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGc                              >  1:2803773/1‑151 (MQ=255)
                                                                                                                    gTAATGTTCTTGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                             <  2:5587802/151‑1 (MQ=255)
                                                                                                                    gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                             <  2:6220064/151‑1 (MQ=255)
                                                                                                                    gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                             <  1:6196352/151‑1 (MQ=255)
                                                                                                                     tAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGa                            <  1:6810653/151‑1 (MQ=255)
                                                                                                                     tAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGa                            >  1:3714518/1‑151 (MQ=255)
                                                                                                                     tAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGa                            >  1:4671905/1‑151 (MQ=255)
                                                                                                                       aTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAcc                          <  2:284658/151‑1 (MQ=255)
                                                                                                                       aTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCCTGTTCAGCAGCATGGCGAcc                          >  2:1475588/1‑151 (MQ=255)
                                                                                                                        tCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCa                         <  1:5820888/151‑1 (MQ=255)
                                                                                                                          ttCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                       >  2:2094012/1‑151 (MQ=255)
                                                                                                                          ttCAGGGGTGAGGGCCGGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                       <  2:3935357/151‑1 (MQ=255)
                                                                                                                             aTGGGTGAGGGCGTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTg                    <  2:3251924/151‑1 (MQ=255)
                                                                                                                             aTGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTCGTTCAGCAGCATGGCGACCAGGTTg                    >  2:1897761/1‑151 (MQ=255)
                                                                                                                              tGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGc                   >  1:2216003/1‑151 (MQ=255)
                                                                                                                              tGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGc                   <  1:6525035/151‑1 (MQ=255)
                                                                                                                              tGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGc                   <  2:6280162/151‑1 (MQ=255)
                                                                                                                                ggTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGa                 <  2:3141034/151‑1 (MQ=255)
                                                                                                                                 gTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGAt                >  1:140630/1‑151 (MQ=255)
                                                                                                                                 gTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGGGCGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTCTTCCGCCGCATGGCGACCAGGTTGCGAt                >  2:4127865/1‑151 (MQ=11)
                                                                                                                                  tGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATg               >  1:1621371/1‑151 (MQ=255)
                                                                                                                                    ggggCCTGGAAAGGGGACGCGGCCGGCGTCAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTGCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGt             <  2:6320760/150‑1 (MQ=11)
                                                                                                                                    aGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGt             <  1:5535617/151‑1 (MQ=255)
                                                                                                                                     gggCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGATACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTg            >  1:6619318/1‑151 (MQ=255)
                                                                                                                                        ccTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGaaa         <  2:4874448/151‑1 (MQ=255)
                                                                                                                                        ccTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGaaa         >  2:5281166/1‑151 (MQ=255)
                                                                                                                                          tGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAgg       >  2:3739272/1‑150 (MQ=255)
                                                                                                                                          tGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGt       <  1:1884126/151‑1 (MQ=255)
                                                                                                                                           ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAggc      >  2:5454014/1‑149 (MQ=255)
                                                                                                                                           ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc      <  2:696072/151‑1 (MQ=255)
                                                                                                                                             aaaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgc    <  1:734539/151‑1 (MQ=255)
                                                                                                                                              aaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgcg   <  2:6999753/151‑1 (MQ=255)
                                                                                                                                              aaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgcg   >  1:1243983/1‑151 (MQ=255)
                                                                                                                                               aTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCgg  >  1:1224351/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGCAAGGCCAGGTTGCGCAGGGCGGCAATCCGCCGTTCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGG  >  CP009974/4544536‑4544829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑