Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,544,703 G→A P140P (CCC→CCT)  RPPX_20290 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,544,7030GA99.2% 845.6 / ‑5.5 251P140P (CCC→CCT) RPPX_20290hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (117/131);  total (118/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

TCCGCCGTTCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCG  >  CP009974/4544564‑4544850
                                                                                                                                           |                                                                                                                                                   
                                      cGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgg                                                                                                    >  2:1629760/1‑151 (MQ=9)
                                      cGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgg                                                                                                    <  1:2917497/151‑1 (MQ=14)
                                       ggTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggg                                                                                                   <  1:4803661/151‑1 (MQ=9)
                                         tATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGTGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggggg                                                                                                 >  1:1729711/1‑151 (MQ=16)
                                         tATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggggg                                                                                                 >  1:3140627/1‑151 (MQ=16)
                                         gaTTTGTCGACATCGAGGCGCGGGGCCAGGCGCGCGCCATTGGGGGAGTAATCTGCATGGGTGAGGGCCTGGAAATGGGACTCGGGCGGCGACAGGGCAGGGGCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggggg                                                                                                 <  2:5279075/150‑1 (MQ=11)
                                             tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGGGAGGGCCTGGAAATGGGACTCGGCCGGCGCCAGGGCAGGCGCCCTGCGTGAAGTGCCCCGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCacc                                                                                             >  2:4969793/1‑149 (MQ=11)
                                              ggcGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                                            <  2:5655491/149‑1 (MQ=16)
                                              gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                                            <  1:3616540/151‑1 (MQ=255)
                                               tCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccg                                                                                           >  1:4718739/1‑151 (MQ=21)
                                                 gACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgcc                                                                                         >  1:6618662/1‑151 (MQ=19)
                                                   cATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCgg                                                                                       <  1:623313/151‑1 (MQ=255)
                                                      ggAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                                                    <  2:2692680/150‑1 (MQ=255)
                                                      cGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                                                    >  2:5330211/1‑151 (MQ=19)
                                                       gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                                                   >  1:1004194/1‑151 (MQ=255)
                                                       gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                                                   <  2:4498295/151‑1 (MQ=255)
                                                       gAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCGTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTAc                                                                                   >  2:1891113/1‑151 (MQ=255)
                                                           cgcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGc                                                                               >  1:1665914/1‑151 (MQ=255)
                                                            gcggggCCAGGGGCGCGCCGGTGGGGGAGTAATCTTCGTGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGct                                                                              <  2:7199635/146‑1 (MQ=11)
                                                              tgtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctct                                                                            >  2:5733572/1‑151 (MQ=255)
                                                              tgtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctct                                                                            >  1:1403202/1‑151 (MQ=255)
                                                               gtgCCAGGCGCGCGCCATTGGGGGAGTAAGCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCtctc                                                                           <  1:2019606/151‑1 (MQ=255)
                                                                tgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTcc                                                                          >  1:2707256/1‑151 (MQ=255)
                                                                tgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTcc                                                                          >  2:1708229/1‑151 (MQ=255)
                                                                 gCCAGGCGCGGGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGGCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGACCGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCa                                                                         <  2:109099/151‑1 (MQ=255)
                                                                     ggCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGt                                                                     <  1:3315351/151‑1 (MQ=255)
                                                                     ggCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGt                                                                     >  1:5282704/1‑151 (MQ=255)
                                                                      gcgcgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTc                                                                    <  1:7064264/151‑1 (MQ=255)
                                                                        gcgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCtt                                                                  >  2:5873025/1‑151 (MQ=255)
                                                                         cgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTg                                                                 >  2:59817/1‑151 (MQ=255)
                                                                         cgcgcCATTGGGGGAGCAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGCACGCGGCCCGCTCCGGTGCGGGGGCACCCGCCCGGTACAGCCTCTGCAGCGTCtcg                                                                 >  2:7090636/1‑149 (MQ=11)
                                                                          gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                                                <  2:1205964/151‑1 (MQ=255)
                                                                          gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                                                >  2:3788709/1‑151 (MQ=255)
                                                                           cgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGtt                                                               <  2:2277926/151‑1 (MQ=255)
                                                                            gcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTc                                                              <  1:2699785/151‑1 (MQ=255)
                                                                             ccATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTca                                                             <  2:5077095/151‑1 (MQ=255)
                                                                             ccATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTca                                                             >  1:2136591/1‑151 (MQ=255)
                                                                              cATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcag                                                            <  1:1797578/151‑1 (MQ=255)
                                                                              cATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcag                                                            <  1:14211/151‑1 (MQ=255)
                                                                               aTTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagc                                                           <  1:6119628/151‑1 (MQ=255)
                                                                               aTTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagc                                                           <  2:3635915/151‑1 (MQ=255)
                                                                                 tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCAGGCCCCCTGCTTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAGGGTCCATCCGCCTGGTACAGGCTCTCCAGCGGCTTGTTcagccg                                                         >  2:1562174/1‑149 (MQ=11)
                                                                                 tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                                         <  1:6434390/151‑1 (MQ=255)
                                                                                 tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                                         <  1:4013865/151‑1 (MQ=255)
                                                                                 tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                                         <  1:818381/151‑1 (MQ=255)
                                                                                  gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCagcagc                                                        <  2:5530585/151‑1 (MQ=255)
                                                                                  gggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCagcagc                                                        <  1:4564184/151‑1 (MQ=255)
                                                                                   ggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAgcagca                                                       <  1:2420912/151‑1 (MQ=255)
                                                                                     ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                                                     >  2:759579/1‑151 (MQ=255)
                                                                                     ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                                                     <  2:4033539/151‑1 (MQ=255)
                                                                                     ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGCCGTGTTCAGCGGCATg                                                     >  1:6728612/1‑151 (MQ=11)
                                                                                      gAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATgg                                                    <  1:6966755/151‑1 (MQ=255)
                                                                                       tgTAATGTTCCTGGGTGAGTGCCTGGAAATGGGTTGCGGCCGGTGACAGGGCAGGCCCCGGGCGTGAAGTGCCCCGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGc                                                   <  2:6933965/150‑1 (MQ=11)
                                                                                       ggTAATCTTCATGGGTGAGGGCGTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGc                                                   <  2:3167379/150‑1 (MQ=255)
                                                                                       aGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGc                                                   >  1:2803773/1‑151 (MQ=255)
                                                                                        gTAATGTTCTTGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                                                  <  2:5587802/151‑1 (MQ=255)
                                                                                        gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                                                  >  2:1334779/1‑151 (MQ=255)
                                                                                        gTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                                                  <  2:6220064/151‑1 (MQ=255)
                                                                                        ctAATCTTCATGGGTAAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCGCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                                                  <  1:4303679/150‑1 (MQ=255)
                                                                                           aTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCCTGTTCAGCAGCATGGCGAcc                                               >  2:1475588/1‑151 (MQ=255)
                                                                                              ttCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                                            <  1:1545337/151‑1 (MQ=255)
                                                                                              ttCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                                            >  2:2094012/1‑151 (MQ=255)
                                                                                              ttCAGGGGTGAGGGCCTGGAAATAGGACTCGGCCGGCGACAGGGCAAGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                                            <  1:626366/151‑1 (MQ=255)
                                                                                              ttCAGGGGTGAGGGCCGGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                                            <  2:3935357/151‑1 (MQ=255)
                                                                                                cATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGtt                                          >  1:4903865/1‑151 (MQ=255)
                                                                                                     gTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGGGCGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTCTTCCGCCGCATGGCGACCAGGTTGCGAt                                     >  2:4127865/1‑151 (MQ=11)
                                                                                                       gAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGAGGCAGCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCACGACGTCCAGGTTGccttag                                   >  2:5488656/1‑146 (MQ=11)
                                                                                                        aGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGt                                  <  2:3496303/151‑1 (MQ=25)
                                                                                                         gggCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTg                                 <  2:6305298/151‑1 (MQ=255)
                                                                                                            ccTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGaaa                              >  1:368568/1‑151 (MQ=255)
                                                                                                             cTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAg                             <  1:4971534/151‑1 (MQ=255)
                                                                                                               ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc                           <  1:2233674/151‑1 (MQ=255)
                                                                                                               ggAAAGGGGACTAAGACGGAGACGGGGCGGACCCCCGGCGTGAAGTGCCCAGCCTGCCCGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGAGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGACAGTc                           <  1:3503490/151‑1 (MQ=11)
                                                                                                                 aaaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgc                         <  1:734539/151‑1 (MQ=255)
                                                                                                                  aaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgcg                        <  2:6999753/151‑1 (MQ=255)
                                                                                                                  aaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgcg                        >  1:1243983/1‑151 (MQ=255)
                                                                                                                   aTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCgg                       >  1:1224351/1‑151 (MQ=255)
                                                                                                                    tgggACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGc                      >  1:1042171/4‑151 (MQ=255)
                                                                                                                        aCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTg                  >  2:5506559/1‑151 (MQ=38)
                                                                                                                        aCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTg                  <  1:5496185/151‑1 (MQ=38)
                                                                                                                         cTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGCTGAAAGTCGCCGCCCTGc                 >  2:5305971/1‑151 (MQ=255)
                                                                                                                           cggccggcGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCgg               >  1:700886/1‑151 (MQ=255)
                                                                                                                           cggccggcGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCgg               >  1:6692492/1‑151 (MQ=255)
                                                                                                                           cggccggcGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCgg               >  1:5560331/1‑151 (MQ=25)
                                                                                                                            ggccggcGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCggg              >  2:6771569/1‑151 (MQ=38)
                                                                                                                             gccggcGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGc             >  2:2528718/1‑151 (MQ=255)
                                                                                                                             gccggcGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGc             <  1:5994540/151‑1 (MQ=255)
                                                                                                                               cggcGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTg           >  1:3893947/1‑151 (MQ=255)
                                                                                                                                 gcGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAg         <  1:2201376/151‑1 (MQ=255)
                                                                                                                                   gACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCt       <  2:829027/151‑1 (MQ=255)
                                                                                                                                   gACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCt       >  1:4505265/1‑151 (MQ=255)
                                                                                                                                   gACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCt       >  2:6201701/1‑151 (MQ=255)
                                                                                                                                    aCAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTg      <  1:3973398/151‑1 (MQ=255)
                                                                                                                                    aCAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCCTGTTCAGCAGCATCGCGACCAGGTTGCGATCGGGAAAGTCGCGGCCCTGCGGGCCGACCTg      >  2:4343204/1‑151 (MQ=11)
                                                                                                                                     cAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGt     >  2:3250759/1‑151 (MQ=255)
                                                                                                                                      aGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGtt    >  2:2857281/1‑151 (MQ=255)
                                                                                                                                       gggcaggcCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTc   <  1:5804021/151‑1 (MQ=255)
                                                                                                                                       gggcaggcCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTc   <  1:6296202/151‑1 (MQ=255)
                                                                                                                                       gggcaggcCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTc   >  2:3318076/1‑151 (MQ=255)
                                                                                                                                        ggcagggCCCGTGCGTGAAGTGGCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCg  <  2:4790128/151‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
TCCGCCGTTCCAGCGCGTCGCGTTCGGCGAGGAAGCGCCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCG  >  CP009974/4544564‑4544850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑