BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,544,907	T→C	T72T (ACA→ACG)	RPPX_20290 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,544,907	0	T	C	99.5%	542.5 / NA	188	T72T (ACA→ACG)	RPPX_20290	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (91/94); total (92/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 196 total aligned reads displayed.

CCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTTGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTGGCGG  >  CP009974/4544759‑4545054

  gccgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTcgtgt                                                                                                                                                 <  1:7180987/151‑1 (MQ=17)

  gccgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGGTGGGCGGGTTTcgtgt                                                                                                                                                 >  1:186436/1‑151 (MQ=17)

    cgGGTACAGGCTCTCCGGCGTCTTGTTCAGCAGGATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGCGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGc                                                                                                                                               <  2:2957163/151‑1 (MQ=9)

    cgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGc                                                                                                                                               <  2:1785794/151‑1 (MQ=14)

    cgGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGc                                                                                                                                               >  1:2824877/1‑151 (MQ=17)

     gggTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCt                                                                                                                                              <  1:4516519/151‑1 (MQ=17)

       gTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGATGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCTCCCCTGCTCGGGAGGTTGGGCGGGTTTAGTGGGccac                                                                                                                                            >  2:5677499/1‑148 (MQ=11)

        tACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGGGGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCGTCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGGTGGGCGGGGTGCGTGTGCTccc                                                                                                                                           >  2:2251269/1‑150 (MQ=11)

        tACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTTAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCa                                                                                                                                           <  2:883480/151‑1 (MQ=14)

        tACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCa                                                                                                                                           >  1:530956/1‑151 (MQ=17)

        tACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCa                                                                                                                                           <  1:3525834/151‑1 (MQ=14)

         aCAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAg                                                                                                                                          >  1:5896743/1‑151 (MQ=17)

           aGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGtt                                                                                                                                        >  1:1000470/1‑151 (MQ=17)

           aGGCGCTCCAGCGTCTTGTGCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGtt                                                                                                                                        <  1:6871557/151‑1 (MQ=9)

            ggCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTg                                                                                                                                       >  1:2907259/1‑151 (MQ=14)

            ggCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTg                                                                                                                                       >  1:1394564/1‑151 (MQ=14)

             gCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGc                                                                                                                                      <  2:3275351/151‑1 (MQ=14)

             gCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGc                                                                                                                                      <  1:4526474/151‑1 (MQ=14)

                ctcCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTcg                                                                                                                                   <  1:5330211/151‑1 (MQ=11)

                ctcCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGGTTCGTGTGCTCCAGTTGCTcg                                                                                                                                   >  2:7015690/1‑151 (MQ=11)

                                                                                 tGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCAt                                                                  >  1:273148/1‑151 (MQ=11)

                                                                                 tGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCAt                                                                  <  2:2803773/151‑1 (MQ=11)

                                                                                 tGAGCTGTGCGGCATCCAGCCGGTAGCATGCCGGCGCGCGCTCCTGCTCGGGAGGTTGGGGGGGTTTCGTGTGCTCCAGTGGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCAt                                                                  <  2:6182108/151‑1 (MQ=255)

                                                                                  gAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATg                                                                 >  2:6051074/1‑151 (MQ=14)

                                                                                   aGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGc                                                                <  1:2948543/151‑1 (MQ=14)

                                                                                    gCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGcc                                                               >  1:5416001/1‑151 (MQ=17)

                                                                                     cTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCt                                                              <  2:559215/151‑1 (MQ=14)

                                                                                      tGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCtt                                                             >  2:4494531/1‑151 (MQ=14)

                                                                                      tGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCtt                                                             >  2:3349942/1‑151 (MQ=14)

                                                                                      tGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCtt                                                             >  1:886178/1‑151 (MQ=17)

                                                                                       gTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtc                                                            <  1:1742114/151‑1 (MQ=17)

                                                                                         tCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcgg                                                          <  1:6771569/151‑1 (MQ=17)

                                                                                          cGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggc                                                         <  1:4405463/151‑1 (MQ=17)

                                                                                           ggCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcg                                                        <  2:1420465/151‑1 (MQ=18)

                                                                                            gCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgt                                                       >  1:5624529/1‑151 (MQ=18)

                                                                                             cATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtc                                                      <  1:3686898/151‑1 (MQ=21)

                                                                                             cATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtc                                                      >  1:502551/1‑151 (MQ=21)

                                                                                             cATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtc                                                      <  1:938197/151‑1 (MQ=21)

                                                                                              aTCCGGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtcg                                                     <  2:4563927/151‑1 (MQ=21)

                                                                                              aTCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtcg                                                     <  1:7211884/151‑1 (MQ=21)

                                                                                               tCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtcgg                                                    <  1:4894136/151‑1 (MQ=21)

                                                                                                 cAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTtcggcgtcggcg                                                  <  1:7243284/151‑1 (MQ=25)

                                                                                                  aGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGa                                                 >  1:6549476/1‑151 (MQ=21)

                                                                                                   gCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAg                                                <  2:5282704/151‑1 (MQ=25)

                                                                                                   gCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGACGCTGGTAGACCGAGACCGACAGGCTCAGGTCCAGGGCCCTGCCTTCGGCGCCGGCGAg                                                >  2:6952051/1‑151 (MQ=11)

                                                                                                    ccGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGc                                               <  2:1021023/151‑1 (MQ=25)

                                                                                                      ggTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcac                                             >  2:6379306/1‑151 (MQ=25)

                                                                                                      ggTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcac                                             >  1:5567603/1‑151 (MQ=25)

                                                                                                       gTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcacc                                            <  2:3252749/151‑1 (MQ=38)

                                                                                                       gTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcacc                                            <  2:5969611/151‑1 (MQ=38)

                                                                                                            aTGCCGGCACTCGCTCCTGCTCGGGAGTTTGGGCGGGTTTCGTGTGCTCCAGTTGCTTGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCATGTCCAGGTCCATGCCTTCGGCGTCGGCGATcaccaccac                                       >  1:2330297/1‑151 (MQ=11)

                                                                                                            aTGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccaccac                                       >  1:1221514/1‑151 (MQ=25)

                                                                                                              gCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccaccacca                                     <  2:5454827/151‑1 (MQ=38)

                                                                                                               ccGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGcaccaccaccac                                    >  1:127834/1‑151 (MQ=38)

                                                                                                                cGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCaccaccaccacc                                   <  1:340242/151‑1 (MQ=38)

                                                                                                                cGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCaccaccaccacc                                   >  2:4821739/1‑151 (MQ=37)

                                                                                                                cGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCaccaccaccacc                                   <  1:1062769/151‑1 (MQ=255)

                                                                                                                 ggCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCg                                  >  2:5987425/1‑151 (MQ=38)

                                                                                                                 ggCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCg                                  >  2:1965762/1‑151 (MQ=38)

                                                                                                                   cACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGc                                <  1:2421039/151‑1 (MQ=255)

                                                                                                                   cACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGc                                >  2:799906/1‑151 (MQ=38)

                                                                                                                    aggcgcTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCa                               <  2:2766595/149‑1 (MQ=255)

                                                                                                                    aCGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCa                               <  2:2755629/151‑1 (MQ=38)

                                                                                                                      gcgcTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACt                             >  1:958403/1‑151 (MQ=38)

                                                                                                                        gcTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACttt                           <  2:4903865/151‑1 (MQ=38)

                                                                                                                        gcTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACttt                           >  2:4733370/1‑151 (MQ=25)

                                                                                                                        gcTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACttt                           <  1:3318076/151‑1 (MQ=255)

                                                                                                                        gcTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACttt                           <  2:1042823/151‑1 (MQ=38)

                                                                                                                         cTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTc                          <  2:1649997/151‑1 (MQ=38)

                                                                                                                         cTCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTc                          >  1:1988344/1‑151 (MQ=255)

                                                                                                                          tCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCGGGGCCATGCGTTCGGCGTCGGCGAGCACCCCCACCACCGGCACTGTCg                         >  2:5404833/1‑151 (MQ=11)

                                                                                                                          tCCTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCg                         <  1:5873025/151‑1 (MQ=255)

                                                                                                                            cTGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGc                       >  1:4176513/1‑151 (MQ=255)

                                                                                                                             tGCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCt                      >  2:610432/1‑151 (MQ=255)

                                                                                                                              gCTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTg                     >  2:4229295/1‑151 (MQ=255)

                                                                                                                               cTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTgg                    <  1:3165005/151‑1 (MQ=255)

                                                                                                                               cTCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCCgg                    >  2:1275688/1‑151 (MQ=255)

                                                                                                                                tggggAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTggg                   <  2:7104464/149‑1 (MQ=255)

                                                                                                                                tCGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTggg                   >  2:6447627/1‑151 (MQ=255)

                                                                                                                                 cGGGAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGt                  <  1:5265354/151‑1 (MQ=255)

                                                                                                                                  gggAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTc                 >  2:698485/1‑151 (MQ=255)

                                                                                                                                    gAGGTTTGTCGGGTTTCGTGTGCTCCATTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGTGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGACACTTTCCGCTGTTTCCa               >  2:2103427/1‑151 (MQ=11)

                                                                                                                                    gAGGTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCa               <  1:4482921/151‑1 (MQ=255)

                                                                                                                                    gAGGTTGGGCGGGTTTCGTGTGCTCCAGTAGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACCTTCGGCTGGGTCCa               >  2:4287659/1‑151 (MQ=255)

                                                                                                                                      ggTTGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAgg             <  2:2518263/151‑1 (MQ=255)

                                                                                                                                      ggTTGGGCGGGGTTCGTGTGCTCCAGTGGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAgg             <  2:4003025/151‑1 (MQ=255)

                                                                                                                                         tGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAggggg          >  1:4004612/1‑150 (MQ=255)

                                                                                                                                         tGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGt          <  1:1749476/151‑1 (MQ=255)

                                                                                                                                         tGGGCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGt          >  2:1725933/1‑151 (MQ=255)

                                                                                                                                          tggCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTa         <  1:4041271/150‑1 (MQ=255)

                                                                                                                                          gggCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTa         >  1:4554946/1‑151 (MQ=255)

                                                                                                                                          gggCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTa         >  2:1568546/1‑151 (MQ=255)

                                                                                                                                           ggCGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAg        <  1:5146932/151‑1 (MQ=255)

                                                                                                                                             cGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCCCTTTCGGCTGGGTCCAGGGGCAGTg      >  2:6595746/1‑151 (MQ=255)

                                                                                                                                             cGGGTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGCAGTg      >  1:2630017/1‑151 (MQ=255)

                                                                                                                                                gTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTggcg   >  1:6591348/1‑151 (MQ=255)

                                                                                                                                                gTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTggcg   >  1:5949552/1‑151 (MQ=255)

                                                                                                                                                gTTTCGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTggcg   >  1:811873/1‑151 (MQ=255)

                                                                                                                                                 tttcGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTggcgg  >  2:5776378/1‑151 (MQ=255)

                                                                                                                                                 tttcGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTggcgg  >  1:6185996/1‑151 (MQ=255)

CCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGGCTCTGCGGGCTGAGCTGTTCGGCATCCAGCCGGTAGCATGCCGGCACGCGCTCCTGCTCGGGAGGTTGGGCGGGTTTTGTGTGCTCCAGTTGCTCGGCCGGCATCAGGTCACGCAGTTGATGCTGGTAGGCCGAGACCGACAGGCTCAGGTCCAGGGCCATGCCTTCGGCGTCGGCGAGCACCACCACCACCGGCACTTTCGGCTGGGTCCAGGGGTAGTGGCGG  >  CP009974/4544759‑4545054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`