Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,007,281 A→T Y68F (TAC→TTC)  RPPX_22185 → phenylacetate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,007,2810AT99.7% 1323.1 / ‑6.7 357Y68F (TAC→TTC) RPPX_22185phenylacetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (179/176);  minor base C (0/1);  total (179/178)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 357 total aligned reads displayed.

AGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACAC  >  CP009974/5007133‑5007428
                                                                                                                                                    |                                                                                                                                                   
aGCGTCGATGCCATGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACGCTGATGACCTCAAGTGCCTGGCAGACCTGGCGAAGTTcc                                                                                                                                                   >  2:5466820/1‑151 (MQ=255)
   gTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCt                                                                                                                                                >  2:2766645/1‑151 (MQ=255)
    tCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCtt                                                                                                                                               <  2:4152624/151‑1 (MQ=255)
    tCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCtt                                                                                                                                               >  2:3912404/1‑151 (MQ=255)
       aTGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCAc                                                                                                                                            <  1:4620490/151‑1 (MQ=255)
        tGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCAcc                                                                                                                                           >  2:856111/1‑151 (MQ=255)
        tGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCAcc                                                                                                                                           <  2:587545/151‑1 (MQ=255)
              gcgcCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAg                                                                                                                                     <  2:3326574/151‑1 (MQ=255)
               cgcCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGa                                                                                                                                    >  1:5544013/1‑151 (MQ=255)
                   aGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGATCTGGCGAAGTTCCCCTTCACCGGCAAGAACGa                                                                                                                                >  1:1572932/1‑151 (MQ=255)
                   aGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGa                                                                                                                                <  1:3951311/151‑1 (MQ=255)
                       cgaGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGGTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTg                                                                                                                            <  2:4539752/149‑1 (MQ=255)
                       ccAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTg                                                                                                                            <  2:5050953/151‑1 (MQ=255)
                        cAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTgc                                                                                                                           <  1:924649/151‑1 (MQ=255)
                         aGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTgcg                                                                                                                          >  1:2867470/1‑151 (MQ=255)
                            tGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGa                                                                                                                       >  1:3586992/1‑151 (MQ=255)
                             ggAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGAc                                                                                                                      >  1:3857705/1‑151 (MQ=255)
                             ggAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGAc                                                                                                                      <  1:1494224/151‑1 (MQ=255)
                              gAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACa                                                                                                                     <  1:4751994/151‑1 (MQ=255)
                              gAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACa                                                                                                                     >  1:4300985/1‑151 (MQ=255)
                                gCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAAc                                                                                                                   <  1:4070389/151‑1 (MQ=255)
                                   tCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTAc                                                                                                                >  1:6579361/1‑151 (MQ=255)
                                      gcgcTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCACAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTAcccc                                                                                                             <  2:3499691/151‑1 (MQ=255)
                                       cgcTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCt                                                                                                            <  2:7102821/151‑1 (MQ=255)
                                       cgcTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCt                                                                                                            <  2:4302131/151‑1 (MQ=255)
                                         cTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCtac                                                                                                          <  1:6784961/151‑3 (MQ=255)
                                            gAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGc                                                                                                       >  1:3148175/1‑151 (MQ=255)
                                            gAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCCGATGACCTCAAGTGCCTGGACGACCTGGCGAAGTTCCCCTTCACCGGCCAGAACGACCTCCGCGACAACTCCCCCtcccgc                                                                                                       >  1:1595633/1‑146 (MQ=255)
                                              gCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCAt                                                                                                     <  2:2495499/151‑1 (MQ=255)
                                                cTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGt                                                                                                   >  1:3033710/1‑151 (MQ=255)
                                                 tGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGtt                                                                                                  >  2:556757/1‑151 (MQ=255)
                                                 tGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGtt                                                                                                  <  1:5309103/151‑1 (MQ=255)
                                                  gAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGttt                                                                                                 <  1:6641123/151‑1 (MQ=255)
                                                   aaGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTg                                                                                                <  1:6972585/151‑1 (MQ=255)
                                                    aGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGc                                                                                               <  2:669600/151‑1 (MQ=255)
                                                    aGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGc                                                                                               <  1:1230199/151‑1 (MQ=255)
                                                    aGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTACACCGGCAAGCACGACCTGCGCGACAACTCCCCCTACGGCATGTTTGc                                                                                               >  2:6484150/1‑151 (MQ=255)
                                                     gCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGcc                                                                                              >  2:1369565/1‑151 (MQ=255)
                                                     gCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTCGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGGCTGTTTGcc                                                                                              >  2:5672256/1‑151 (MQ=255)
                                                      cACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCg                                                                                             >  1:5672956/1‑151 (MQ=255)
                                                         gCCTACGACAATGTGCCGTTGTACCGCCCGCGCCTCGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTCCCCCTTCACCGGCAAGAACCACCTGCGCGACAACTACCCCTCCGGCATGTTCGCCGTGc                                                                                          <  2:1165515/151‑1 (MQ=255)
                                                          ccTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGcc                                                                                         >  1:2102509/1‑151 (MQ=255)
                                                          ccTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGcc                                                                                         <  1:3713385/151‑1 (MQ=255)
                                                           cTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGGACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGccc                                                                                        <  2:1914904/151‑1 (MQ=255)
                                                             aCGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCa                                                                                      >  1:5181134/1‑151 (MQ=255)
                                                               gACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAgg                                                                                    >  2:6177964/1‑151 (MQ=255)
                                                                aCAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGga                                                                                   <  1:6854079/151‑1 (MQ=255)
                                                                 cAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaa                                                                                  >  2:6254702/1‑151 (MQ=255)
                                                                 cAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaa                                                                                  >  2:3552416/1‑151 (MQ=255)
                                                                 cAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaa                                                                                  >  2:7136993/1‑151 (MQ=255)
                                                                   aTGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaaga                                                                                <  1:5765388/151‑1 (MQ=255)
                                                                   aTGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaaga                                                                                <  1:1634220/151‑1 (MQ=255)
                                                                    tgtgCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaagaa                                                                               <  2:1978371/151‑1 (MQ=255)
                                                                      tgCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgt                                                                             <  1:6341408/151‑1 (MQ=255)
                                                                          gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                         <  2:6725896/151‑1 (MQ=255)
                                                                           ttGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgc                                                                        <  2:4803491/151‑1 (MQ=255)
                                                                            tGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGGgcg                                                                       >  1:4442551/1‑151 (MQ=255)
                                                                                 cGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGc                                                                  >  1:236541/1‑151 (MQ=255)
                                                                                    cAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATg                                                               >  2:1445064/1‑151 (MQ=255)
                                                                                    cAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATg                                                               >  1:1075582/1‑151 (MQ=255)
                                                                                     aGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTTCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGc                                                              >  1:180850/1‑151 (MQ=255)
                                                                                     aGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGc                                                              >  2:700849/1‑151 (MQ=255)
                                                                                       cgcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGccc                                                            >  2:4407743/1‑150 (MQ=255)
                                                                                        gcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTc                                                           >  2:285691/1‑151 (MQ=255)
                                                                                                     cGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCaccacc                                              <  1:856111/151‑1 (MQ=255)
                                                                                                     cGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCaccacc                                              <  2:3033710/151‑1 (MQ=255)
                                                                                                     cGGCAACCACCCTGATGACCGCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCaccacc                                              <  2:1224407/151‑1 (MQ=255)
                                                                                                      ggCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCACGCCTCCAGTGGCaccaccc                                             >  2:1741021/1‑150 (MQ=255)
                                                                                                         acccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCa                                          >  1:5630600/1‑151 (MQ=255)
                                                                                                          cccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCaa                                         >  1:4648030/1‑151 (MQ=255)
                                                                                                          cccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCaa                                         >  1:4497844/1‑151 (MQ=255)
                                                                                                          cccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACCACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCaa                                         <  1:6751792/151‑1 (MQ=255)
                                                                                                           ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                        <  1:3325618/151‑1 (MQ=255)
                                                                                                           ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                        >  1:3347668/1‑151 (MQ=255)
                                                                                                             acccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGcc                                      <  1:1378580/151‑1 (MQ=255)
                                                                                                              cccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCg                                     >  2:434099/1‑151 (MQ=255)
                                                                                                                 tgatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACg                                  >  2:1783980/1‑151 (MQ=255)
                                                                                                                  gatggCCGCAGGTGCCTGGAAGACCTGGCGAAGTTCCGCTTGGCCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACgg                                 <  2:6349268/151‑1 (MQ=255)
                                                                                                                   atgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggt                                >  1:2551374/1‑151 (MQ=255)
                                                                                                                     gaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtgg                              <  1:4194456/151‑1 (MQ=255)
                                                                                                                       ccTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTc                            <  1:4950729/151‑1 (MQ=255)
                                                                                                                        cTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCg                           <  2:3754657/151‑1 (MQ=255)
                                                                                                                        cTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCg                           >  2:2295138/1‑151 (MQ=255)
                                                                                                                         tCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCgg                          >  2:6569734/1‑151 (MQ=255)
                                                                                                                         tCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCgg                          >  1:6168716/1‑151 (MQ=255)
                                                                                                                          cAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                         <  1:5050587/151‑1 (MQ=255)
                                                                                                                          cAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                         <  2:163682/151‑1 (MQ=255)
                                                                                                                          cAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                         <  1:7106868/151‑1 (MQ=255)
                                                                                                                           aaGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCt                        >  2:4909301/1‑151 (MQ=255)
                                                                                                                           aaGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCt                        <  2:4658437/151‑1 (MQ=255)
                                                                                                                                ccTGGAAGACCTGGCGAAGTGCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAcc                   <  2:4762119/151‑1 (MQ=255)
                                                                                                                                  tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                 <  1:1444957/151‑1 (MQ=255)
                                                                                                                                  tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                 <  1:1181901/151‑1 (MQ=255)
                                                                                                                                    gAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGa               <  1:5713702/151‑1 (MQ=255)
                                                                                                                                     aaGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGaa              >  2:5216178/1‑151 (MQ=255)
                                                                                                                                      aGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGTCCTGCGCGACAACTACCCCTACGGCATGGTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAAc             <  2:5512954/151‑1 (MQ=255)
                                                                                                                                        aCCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGACCgg           >  2:5103813/1‑150 (MQ=255)
                                                                                                                                          cTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGata         <  2:270581/151‑1 (MQ=255)
                                                                                                                                            ggCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATc       <  2:2241337/151‑1 (MQ=255)
                                                                                                                                            ggCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATc       >  2:7213552/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACAC  >  CP009974/5007133‑5007428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑