Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,305,850 A→G I115V (ATA→GTA)  RPPX_23590 → leucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,305,8500AG99.8% 1424.3 / NA 394I115V (ATA→GTA) RPPX_23590leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (217/175);  total (217/177)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 394 total aligned reads displayed.

GAGCCAGTTGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGATAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCACTTCGCCGACC  >  CP009974/5305702‑5305997
                                                                                                                                                    |                                                                                                                                                   
gAGCCAGTTGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTa                                                                                                                                                   <  1:1084791/151‑1 (MQ=255)
gAGCCAGTTGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTa                                                                                                                                                   <  1:4009234/151‑1 (MQ=255)
 aGCCAGTTGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTaa                                                                                                                                                  <  2:1598427/151‑1 (MQ=255)
    cAGTTGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAAccc                                                                                                                                               <  2:3785844/151‑1 (MQ=255)
     aGTTGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCcg                                                                                                                                              <  2:2756837/151‑1 (MQ=255)
       gtGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGGCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACAcgcg                                                                                                                                            <  2:6099631/150‑1 (MQ=255)
        tGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGa                                                                                                                                           <  2:6907659/151‑1 (MQ=255)
        gggAGCGGGGCTGTCGCGACCGGGGCGAACGGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGa                                                                                                                                           <  2:6216931/150‑1 (MQ=255)
         ggAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAg                                                                                                                                          <  1:2676264/151‑1 (MQ=255)
         ggAGCAGGCCGCTCGCTGCCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAg                                                                                                                                          <  2:3118792/151‑1 (MQ=255)
          gAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAgg                                                                                                                                         <  2:5922496/151‑1 (MQ=255)
           ggCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGgc                                                                                                                                        <  2:2387397/150‑1 (MQ=255)
           aGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGgc                                                                                                                                        >  2:1522790/1‑151 (MQ=255)
           aGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGgc                                                                                                                                        <  1:3629687/151‑1 (MQ=255)
           aGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGgc                                                                                                                                        <  1:6795390/151‑1 (MQ=255)
              aGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCa                                                                                                                                     >  1:2351310/1‑151 (MQ=255)
              aGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCa                                                                                                                                     >  2:530990/1‑151 (MQ=255)
              aGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCa                                                                                                                                     >  2:6866893/1‑151 (MQ=255)
                    ctcGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAACGCTGGCCGGTGACCGGCTGGGCAGCGGGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACtt                                                                                                                               >  2:1121040/1‑151 (MQ=255)
                       gCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGcc                                                                                                                            >  1:5412478/1‑150 (MQ=255)
                       gCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCa                                                                                                                            >  1:1597455/1‑151 (MQ=255)
                        cTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCAc                                                                                                                           <  2:1511659/151‑1 (MQ=255)
                         tACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCAcg                                                                                                                          <  2:4560470/151‑1 (MQ=255)
                         tACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGCCGCATCACTTGCAcc                                                                                                                          >  2:4383174/1‑150 (MQ=255)
                         gggcGTGGGGAACAGCGGTGGAACCTGCTGTATGAGGTGCTGTGGCGTGTCGCCCATGGCGGCCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCAcg                                                                                                                          <  2:2629004/148‑1 (MQ=255)
                          aCCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGCGAGCTGCCACGGCGCATCAAGCCGGGAACCCGCGAGGCGCAACACTTGCCcgc                                                                                                                         >  2:1409374/1‑151 (MQ=255)
                           ccGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCAcgcg                                                                                                                        <  1:936937/151‑1 (MQ=255)
                             gTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGtt                                                                                                                      <  1:4265730/151‑1 (MQ=255)
                             gTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGtt                                                                                                                      >  1:2898481/1‑151 (MQ=255)
                                     cAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCt                                                                                                              <  2:5499644/151‑1 (MQ=255)
                                      aGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCtt                                                                                                             <  1:5288537/151‑1 (MQ=255)
                                       gcgcTGGAACCTGCGGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGACGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTc                                                                                                            <  1:1554700/151‑1 (MQ=255)
                                        cgcTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCg                                                                                                           >  1:5534698/1‑151 (MQ=255)
                                         gcTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCCTCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTTTACGCTTCGt                                                                                                          >  1:3306432/1‑151 (MQ=255)
                                          cggggaCCTGCGGTATGAGGGGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGCCCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTg                                                                                                         <  1:3268046/146‑1 (MQ=255)
                                            ggAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGcc                                                                                                       >  2:6013801/1‑151 (MQ=255)
                                             gAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCg                                                                                                      <  1:6601939/151‑1 (MQ=255)
                                              aaCCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGt                                                                                                     <  1:4542713/151‑1 (MQ=255)
                                               gccggctgTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGtt                                                                                                    <  2:3848266/147‑1 (MQ=255)
                                                ccTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCCGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCGTCGTGCCGTTg                                                                                                   >  2:2966029/1‑151 (MQ=255)
                                                 ctgcggTCTGAGGTGCTGTAGCGCGTCGCTAATGGCGCCCGCAGGGCAGTGCTGGCCGGTCACCGGCTGGGCAGTGAGCGGAAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTGGTACGCTTCGTGCCGTTGc                                                                                                  <  2:3583349/151‑1 (MQ=255)
                                                    ctgTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTg                                                                                               <  1:6503321/151‑1 (MQ=255)
                                                      gTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTgcg                                                                                             >  2:3043086/1‑149 (MQ=255)
                                                       tATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAgg                                                                                            >  1:2255912/1‑151 (MQ=255)
                                                        aTGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGa                                                                                           >  1:3514084/1‑151 (MQ=255)
                                                           aaGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACt                                                                                        <  1:4300832/151‑1 (MQ=255)
                                                              tGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGc                                                                                     >  1:1713746/1‑151 (MQ=255)
                                                                cTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCGGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGGGTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCag                                                                                   >  2:2039200/1‑151 (MQ=255)
                                                                  gtgGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCagag                                                                                 <  1:3056868/151‑1 (MQ=255)
                                                                  gtgGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCagag                                                                                 >  2:2465302/1‑151 (MQ=255)
                                                                      cGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACt                                                                             >  2:667008/1‑151 (MQ=255)
                                                                       gtgtCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACtt                                                                            >  2:5183352/1‑151 (MQ=255)
                                                                        tgtCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTg                                                                           >  2:6464915/1‑151 (MQ=255)
                                                                           cGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAg                                                                        >  1:801660/1‑151 (MQ=255)
                                                                             cccATGGCGCCCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCt                                                                      <  1:1307132/151‑1 (MQ=255)
                                                                               ccgggCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTgg                                                                    <  2:4210415/148‑1 (MQ=255)
                                                                                 tGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGAt                                                                  <  2:6542936/151‑1 (MQ=255)
                                                                                 tGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCACAGCACTTGCAGCTGGAt                                                                  >  1:4973238/1‑151 (MQ=255)
                                                                                     gACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCCTCCAGCAGGTACCCCGCGCGGCGCATCACTTGCACGCGCTCGTACGCTTCGTGCCGTCGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGGTCtgg                                                              >  2:855561/1‑151 (MQ=255)
                                                                                       ccGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCtggtg                                                            <  1:582586/151‑1 (MQ=255)
                                                                                        cGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCtggtgg                                                           >  1:6214049/1‑151 (MQ=255)
                                                                                            cGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTa                                                       >  1:5908167/1‑151 (MQ=255)
                                                                                              gCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATc                                                     >  1:1430232/1‑151 (MQ=255)
                                                                                                 aTGCTTGGCGGTGACCGGGTGGGAAGTGGGCTTCAACGGGGCATCAATCAGATAACCCGCGCGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACg                                                  <  1:7204281/151‑1 (MQ=255)
                                                                                                  tGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGa                                                 >  2:4904427/1‑151 (MQ=255)
                                                                                                  tGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGa                                                 <  1:1457369/151‑1 (MQ=255)
                                                                                                   gCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAg                                                >  1:5173184/1‑151 (MQ=255)
                                                                                                      ggCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCg                                             <  1:2584922/151‑1 (MQ=255)
                                                                                                      ggCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCg                                             <  1:4085906/151‑1 (MQ=255)
                                                                                                        ccGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTCCAGCTGGATCTGGTGGCCTATCACGAGCCCGc                                           >  2:4066211/1‑151 (MQ=255)
                                                                                                        ccGGTGACCGGCGGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGc                                           <  2:1224004/151‑1 (MQ=255)
                                                                                                         cGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATAACGCGGCGGca                                          >  2:5766521/1‑151 (MQ=255)
                                                                                                         cGGTGACCGGCGGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGca                                          <  2:3729548/151‑1 (MQ=255)
                                                                                                          ggTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGcac                                         <  1:177116/151‑1 (MQ=255)
                                                                                                          ggTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGcac                                         >  1:2016400/1‑151 (MQ=255)
                                                                                                             gACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGCGCCGGCacacc                                      >  2:4799512/1‑150 (MQ=255)
                                                                                                             gACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGCTTGTACGCTTCGTGCCGTTGCCAGAGGAAGTGGCAGAGCCCTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACg                                      >  2:7231884/1‑151 (MQ=255)
                                                                                                                 ggCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACAt                                  >  2:5247187/1‑151 (MQ=255)
                                                                                                                   cTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTc                                >  1:2436416/1‑151 (MQ=255)
                                                                                                                   cTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTc                                >  2:1267005/1‑151 (MQ=255)
                                                                                                                     gggCAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGCAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTCTCACCAGCCGGCACACGACATTccg                              >  1:2082315/1‑149 (MQ=255)
                                                                                                                        cAGTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGcac                           >  1:6399195/1‑151 (MQ=255)
                                                                                                                          gTGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCAcag                         <  1:3489774/151‑1 (MQ=255)
                                                                                                                           tGAGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCAcagc                        >  1:3749227/1‑151 (MQ=255)
                                                                                                                             aGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCAcagcgc                      >  1:116504/1‑151 (MQ=255)
                                                                                                                             aGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCAcagcgc                      >  1:3835973/1‑151 (MQ=255)
                                                                                                                             aGCTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCAcagcgc                      <  1:4508379/151‑1 (MQ=255)
                                                                                                                               cTGCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCAcagcgcca                    <  1:4202450/151‑1 (MQ=255)
                                                                                                                               cTGCAACGGCACATCAAGCAGGTACCCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTTGCCTATCACGAGCCGGCACACGACATTCTGCAcagcgcca                    <  1:2768743/151‑1 (MQ=255)
                                                                                                                                 gCAACGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCAcagcgccagc                  <  1:2919700/151‑1 (MQ=255)
                                                                                                                                   aaCGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCAcagcgccagcgc                >  2:2238319/1‑151 (MQ=255)
                                                                                                                                    aCGGCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACagcgccagcgcc               >  2:1964015/1‑151 (MQ=255)
                                                                                                                                      ggCGCATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCa             >  2:4638967/1‑151 (MQ=255)
                                                                                                                                       gcgcATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCTGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCAc            <  1:6518607/151‑1 (MQ=255)
                                                                                                                                       gcgcATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCAc            >  2:4991328/1‑151 (MQ=255)
                                                                                                                                         gcATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCACtt          >  2:628708/1‑151 (MQ=255)
                                                                                                                                          cATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCACTTc         <  2:3346119/151‑1 (MQ=255)
                                                                                                                                          cATCAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCACTTc         >  1:7074291/1‑151 (MQ=255)
                                                                                                                                             cAAGCAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCACTTCGcc      <  2:2106732/151‑1 (MQ=255)
                                                                                                                                                 cAGGTAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCACTTCGCCGAcc  <  1:2140525/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGCCAGTTGGAGCAGGCCTCTCGCTACCGTGGCGAACAGCGCTGGAACCTGCTGTATGAAGTGCTGTGGCGTGTCGCCCATGGCGACCGCACGGCAATGCTGGCCGGTGACCGGCTGGGCAGTGAGCTGCAACGGCGCATCAAGCAGATAACCCGCGAGGCGCATCACTTGCACGCGTTTGTACGCTTCGTGCCGTTGCCTGAGGAACTGGCAGAGCACTTGCAGCTGGATCTGGTGGCCTATCACGAGCCGGCACACGACATTCTGCACAGCGCCAGCGCCCACTTCGCCGACC  >  CP009974/5305702‑5305997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑