Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,595,953 T→C L225P (CTC→CCC)  RPPX_24915 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,595,9530TC99.2% 934.9 / ‑2.8 394L225P (CTC→CCC) RPPX_24915hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (203/188);  minor base G (2/1);  total (205/189)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 396 total aligned reads displayed.

CAGCCCCATGTGCTGCCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCTCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGGAACACACAAAACCCACCCA  >  CP009974/5595805‑5596100
                                                                                                                                                    |                                                                                                                                                   
 aGCCCCATGTGCTGCCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTg                                                                                                                                                  <  1:276193/151‑1 (MQ=14)
  gCCCCATGTGCTGCCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTgg                                                                                                                                                 >  1:5196637/1‑151 (MQ=17)
  gCCCCATGTGCTGCCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTgg                                                                                                                                                 >  1:83078/1‑151 (MQ=17)
   ccccATGTGCTGCCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGa                                                                                                                                                <  2:7133955/151‑1 (MQ=14)
    cccATGTGCTGCCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGAc                                                                                                                                               >  2:949160/1‑151 (MQ=17)
     ccATGTGCTGCCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGAcc                                                                                                                                              >  1:2006301/1‑151 (MQ=17)
          tgctgcCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAAcc                                                                                                                                         <  1:2315846/151‑1 (MQ=14)
            ctgcCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGa                                                                                                                                       >  1:4608533/1‑151 (MQ=17)
            ctgcCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGa                                                                                                                                       >  1:2433372/1‑151 (MQ=14)
            ctgcCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGa                                                                                                                                       >  1:1225654/1‑151 (MQ=17)
             tgcCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGaa                                                                                                                                      >  1:551076/1‑151 (MQ=14)
              gcCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGaaa                                                                                                                                     >  1:1750830/1‑151 (MQ=17)
                cagtcagtGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGt                                                                                                                                   >  1:1009521/1‑151 (MQ=17)
                    cagtGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCg                                                                                                                               <  2:2840071/151‑1 (MQ=17)
                       tGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCggtg                                                                                                                            >  2:4204010/1‑151 (MQ=17)
                       tGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCggtg                                                                                                                            >  2:4421176/1‑151 (MQ=14)
                         cccTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCggtggt                                                                                                                          <  1:1615235/151‑1 (MQ=17)
                            tGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACTCAACCTAAAGTGCCggtggtggt                                                                                                                       >  1:3320274/1‑151 (MQ=9)
                             gCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGgtggtggtg                                                                                                                      <  1:3472563/151‑1 (MQ=17)
                                aaCCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGgcg                                                                                                                   >  2:1040059/1‑151 (MQ=17)
                                   cTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTc                                                                                                                <  2:209945/151‑1 (MQ=17)
                                   cTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTc                                                                                                                <  2:1346888/151‑1 (MQ=17)
                                   cTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTc                                                                                                                <  1:6999070/151‑1 (MQ=17)
                                     gggTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGc                                                                                                              >  2:3584791/1‑151 (MQ=17)
                                     gggTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGc                                                                                                              <  2:5512650/151‑1 (MQ=17)
                                      ggTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGcc                                                                                                             >  2:1429634/1‑151 (MQ=14)
                                      ggTCGAAGACCGCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGGGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGcc                                                                                                             <  1:3006102/151‑1 (MQ=9)
                                       gTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCg                                                                                                            >  2:228828/1‑151 (MQ=14)
                                         cGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGAt                                                                                                          <  2:3781826/151‑1 (MQ=17)
                                          gAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATg                                                                                                         >  2:6842238/1‑151 (MQ=17)
                                           aaGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGc                                                                                                        <  2:6092516/151‑1 (MQ=14)
                                            aGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGcc                                                                                                       <  1:5977965/151‑1 (MQ=17)
                                            aGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGcc                                                                                                       <  1:2212304/151‑1 (MQ=17)
                                             gACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGCAAGTGCCGGTGGTGGTGGCGCTCGCCGATGccc                                                                                                      >  2:5179350/1‑150 (MQ=12)
                                                   aaGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGCAgtcc                                                                                                >  2:40183/1‑148 (MQ=9)
                                                      ccAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCg                                                                                                      >  1:1818387/1‑142 (MQ=17)
                                                      ccAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATgg                                                                                             <  1:4819857/151‑1 (MQ=21)
                                                       cAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGc                                                                                            <  1:5347378/151‑1 (MQ=21)
                                                           cGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTg                                                                                        >  1:1542446/1‑151 (MQ=25)
                                                           cGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATAACCGACACCTTGCCCATCGGCTACCTCACCCCCATCGCCCGGCACTACCCCTGAACCCAACCGAACGTGCCGGTGCCGGTCGCGCTCCCCGATGCCGACCGCCTGGccgcc                                                                                        >  2:6463089/1‑148 (MQ=255)
                                                            gAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTgg                                                                                       <  1:1068004/151‑1 (MQ=25)
                                                              gcggcgCATGCCGCTAACCTGGAGCCGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGAc                                                                                     <  2:4387830/151‑1 (MQ=21)
                                                              gcggcgCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGAc                                                                                     >  1:1448819/1‑151 (MQ=21)
                                                               cggcgCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGAcc                                                                                    <  2:1240421/151‑1 (MQ=21)
                                                                ggcgCATGCCGCTAACCTGGAGCAGCGACTCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCt                                                                                   <  1:6441315/151‑1 (MQ=25)
                                                                ggcgCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCt                                                                                   <  1:3996085/151‑1 (MQ=21)
                                                                ggcgCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCt                                                                                   >  1:4077752/1‑151 (MQ=25)
                                                                 gcgcATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCACGGCCCTGGACCTg                                                                                  >  2:5244246/1‑151 (MQ=18)
                                                                   gcATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAg                                                                                >  2:4951127/1‑151 (MQ=21)
                                                                     aTGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGcc                                                                              <  2:5802867/151‑1 (MQ=21)
                                                                      tGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCt                                                                             <  2:3086055/151‑1 (MQ=25)
                                                                       gCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTg                                                                            <  1:6280683/151‑1 (MQ=21)
                                                                        ccGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGt                                                                           <  2:5599220/151‑1 (MQ=25)
                                                                        ccGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGt                                                                           >  1:6324741/1‑151 (MQ=25)
                                                                         cGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCCCTGCCATCGCCCGGCACTACCCCGGGACCCAACCGACAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTc                                                                          <  2:3241841/151‑1 (MQ=255)
                                                                         cGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTc                                                                          >  1:3875088/1‑151 (MQ=21)
                                                                         cGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTc                                                                          <  2:2668417/151‑1 (MQ=25)
                                                                           ctgaCCGGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGGGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCgg                                                                        <  2:7037843/148‑1 (MQ=0)
                                                                           cTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCgg                                                                        >  1:3174373/1‑151 (MQ=21)
                                                                             aaCCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGtc                                                                      >  2:1472629/1‑151 (MQ=17)
                                                                             aaCCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGtc                                                                      >  1:2348030/1‑151 (MQ=18)
                                                                              aCCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCTATCGCCCGGCACTACCCCTGGATCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGCCCTGAGCCTGTCGGtct                                                                     >  2:2931036/1‑151 (MQ=9)
                                                                              aCCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGtct                                                                     >  1:748835/1‑151 (MQ=14)
                                                                               ccTGGAGCCGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCATCCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGtctc                                                                    <  2:3865659/151‑1 (MQ=12)
                                                                                    agcagcGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCt                                                               >  1:448407/1‑151 (MQ=17)
                                                                                     gcagcGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTa                                                              <  2:6535640/151‑1 (MQ=17)
                                                                                     gcagcGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTa                                                              >  1:5733549/1‑151 (MQ=17)
                                                                                      cagcGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTAc                                                             >  1:1336121/1‑151 (MQ=17)
                                                                                       agcGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTAcc                                                            <  2:8644/151‑1 (MQ=17)
                                                                                       agcGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTAcc                                                            <  2:6765645/151‑1 (MQ=14)
                                                                                         cgccccGAGCACCGCCACCCTGCCCATCGGCAACCTCCCTGCCAGCGCCCGGCACTACCCGTGGACCCGACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAGGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAg                                                          <  2:5893810/148‑1 (MQ=11)
                                                                                             ccGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATc                                                      <  2:2509259/151‑1 (MQ=17)
                                                                                               gATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCaa                                                    >  2:4333905/1‑151 (MQ=17)
                                                                                                  cACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTg                                                 >  2:3404854/1‑151 (MQ=14)
                                                                                                      gAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTg                                             <  1:4333905/151‑1 (MQ=17)
                                                                                                         cccTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGAcc                                          <  2:3937122/150‑1 (MQ=14)
                                                                                                          ccTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCt                                         >  2:7089557/1‑151 (MQ=14)
                                                                                                           cTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTg                                        <  2:2476754/151‑1 (MQ=17)
                                                                                                           cTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTCGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTCGACCTTAGCCTGTCGGTCTCGGCCTACCAGCATCGACTGCGTGccccg                                        >  2:5637719/1‑149 (MQ=11)
                                                                                                            ttGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGa                                       <  1:6160170/151‑1 (MQ=17)
                                                                                                              gCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATg                                     <  2:4610372/151‑1 (MQ=17)
                                                                                                               cccATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATgc                                    >  1:6577260/1‑151 (MQ=14)
                                                                                                                ccATCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATgcc                                   <  1:445415/151‑1 (MQ=17)
                                                                                                                  aTCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATgccgg                                 <  2:1631254/151‑1 (MQ=17)
                                                                                                                  aTCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATgccgg                                 <  1:5892452/151‑1 (MQ=17)
                                                                                                                  aTCGGCAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATgccgg                                 >  1:4883692/1‑151 (MQ=14)
                                                                                                                     ggCACCCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATgccggccg                              <  2:1824810/151‑1 (MQ=14)
                                                                                                                       cAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAg                            >  1:3668584/1‑151 (MQ=17)
                                                                                                                       cAACCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAg                            >  1:6800623/1‑151 (MQ=17)
                                                                                                                        aaCCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGc                           >  2:5407263/1‑151 (MQ=17)
                                                                                                                         aCCTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCa                          >  1:5018796/1‑151 (MQ=17)
                                                                                                                          ccTCACTGCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCaa                         <  2:3447969/151‑1 (MQ=17)
                                                                                                                           cTCGCTGCCATCGCCCGGCACTACCCCTGGACCCGACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAAc                        <  2:1698232/151‑1 (MQ=12)
                                                                                                                                 gCCATCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGGAac                  <  2:2592512/151‑1 (MQ=18)
                                                                                                                                    aTCGCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGGAacaca               <  2:6306368/151‑1 (MQ=21)
                                                                                                                                       gCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGGAACACACaa            >  2:1960588/1‑151 (MQ=18)
                                                                                                                                       gCCCGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGGAACACACaa            <  1:6210556/151‑1 (MQ=25)
                                                                                                                                        cccGGCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGCAACACACaaa           >  2:5115884/1‑151 (MQ=21)
                                                                                                                                            gCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGGAACACACAAAaccc       <  1:5614255/151‑1 (MQ=25)
                                                                                                                                            gCACTACCCCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGGAACACACAAAaccc       <  2:819063/151‑1 (MQ=25)
                                                                                                                                                    |                                                                                                                                                   
CAGCCCCATGTGCTGCCAGTCAGTGCCCTGCAAACCTGGGTCGAAGACTTCAAGCCAACCGAGCGGCGCATGCCGCTAACCTGGAGCAGCGACCCGATCACCGAAACCTTGCCCATCGGCAACCTCACTGCCATCGCCCGGCACTACCTCTGGACCCAACCGAAAGTGCCGGTGGTGGTGGCGCTCGCCGATGCCGAAGGCATGGCCCTGGACCTGAGCCTGTCGGTCTCGGCCTACCAGCATCAACTGCGTGACCTGATGCCGGCCGAGCAACTGGAACACACAAAACCCACCCA  >  CP009974/5595805‑5596100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑