Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	768,193	A→G	G462G (GGT→GGC)	RPPX_03350 ←	acetolactate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	768,193	0	A	G	94.4%	24.7 / ‑3.8	18	G462G (GGT→GGC)	RPPX_03350	acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (13/4);  total (13/5)
Fisher's exact test for biased strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

GCCGTTTGCGCCGTAGGCTTCGGCATACTTGACGAAGTCCGGGTTGCCGTAGTCCAGGCCGAAATCGGTGAAGCCCATGTTGGCCTGCTTCCAGCGGATCATGCCGTAACCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGAT  >  CP009974/768085‑768340                                                                                                             |                                                                                                                                                    gcggTTTGCGCCGTAGGCTTCGGCATACTTGACGAAGTCCGGGTTGCCGTAGTCCAGGCCGAAATCGGTGAAGCCCATGTTGGCCTGCTTCCAGCGGATCATGCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGa                                                                                                           <  2:3489073/148‑1 (MQ=1)                                                    gtgcAGGCCGAAATCGGTGAAGCCCATGTTGGCCTGCTTCCAGCGGATCATGCCGTAACCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCca                                                        <  2:6174610/148‑1 (MQ=1)                                                                                                    cATGCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCAtt        >  1:3486423/1‑151 (MQ=11)                                                                                                    cATGCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCAtt        >  2:2535483/1‑151 (MQ=11)                                                                                                      tGCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGc      >  1:896354/1‑151 (MQ=14)                                                                                                       gacGTAGCGGTCGAGACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTAGTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCTTCGCCACACACCGGGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGcc     <  2:1222936/149‑1 (MQ=11)                                                                                                       gCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGcc     >  2:2676876/1‑151 (MQ=14)                                                                                                       gCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGcc     >  2:2677445/1‑151 (MQ=14)                                                                                                       gCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGcc     >  1:2990831/1‑151 (MQ=17)                                                                                                       gCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGcc     >  2:2671208/1‑151 (MQ=14)                                                                                                       gCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGcc     >  1:3639912/1‑151 (MQ=14)                                                                                                       gCCGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGcc     >  1:3639876/1‑151 (MQ=14)                                                                                                       gCCGTAGCCGTCGTCACGCAGCATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCGGCCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGCTTGCCGGGCGGTCCGGGCCCCCCAGGGCCGCCGCCATTGcc     >  2:4424301/1‑151 (MQ=11)                                                                                                        ccGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCg    >  1:5082719/1‑151 (MQ=14)                                                                                                         cGTAGCTGTCGGCACGCAGGATCACCACGGTGATGTGCATGCCCGGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCAGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGa   <  2:6267371/151‑1 (MQ=255)                                                                                                         cGTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACCCACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGa   >  2:3095112/1‑151 (MQ=14)                                                                                                          gTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGAt  <  2:6687904/151‑1 (MQ=17)                                                                                                          gTAGCCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGACCAGGCATTGCCGAt  >  2:5268683/1‑151 (MQ=9)                                                                                                             |                                                                                                                                                    GCCGTTTGCGCCGTAGGCTTCGGCATACTTGACGAAGTCCGGGTTGCCGTAGTCCAGGCCGAAATCGGTGAAGCCCATGTTGGCCTGCTTCCAGCGGATCATGCCGTAACCGTCGTCACGCAGGATCACCACGGTGATGTGCATGCCCAGACGTACTGCAGTTTCCAGTTCCTGGCTGTTCATCATGAAGCCGCCGTCGCCACACACCGAGATTACCGGGCGGTCCGGGTGCACCAGGTGCGCAGCCATTGCCGAT  >  CP009974/768085‑768340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑