Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 775,456 G→A intergenic (‑218/‑240) RPPX_03375 ← / → RPPX_03380 16S ribosomal RNA/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974775,4560GA100.0% 802.8 / NA 242intergenic (‑218/‑240)RPPX_03375/RPPX_0338016S ribosomal RNA/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (132/110);  total (132/110)

Only 100 of 246 total aligned reads displayed.

GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCT  >  CP009974/775347‑775603
                                                                                                             |                                                                                                                                                   
gTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttc                                                                                                            <  1:3533901/151‑1 (MQ=255)
 tCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttct                                                                                                           >  1:1218442/1‑151 (MQ=11)
  ccGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttctt                                                                                                          >  2:4372464/1‑151 (MQ=255)
       cTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaa                                                                                                     <  1:6184889/151‑1 (MQ=255)
        tCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaaa                                                                                                    >  2:6800001/1‑151 (MQ=255)
           caAGCGCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCt                                                                                                 <  2:5109472/151‑1 (MQ=11)
           caAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCt                                                                                                 <  1:2381239/151‑1 (MQ=11)
            aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaa                                                                                                     >  2:3300261/1‑146 (MQ=11)
              gCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaac                                                                                              <  1:109069/151‑1 (MQ=14)
              gCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaac                                                                                              >  1:1046784/1‑151 (MQ=255)
              gCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaac                                                                                              >  2:5430724/1‑151 (MQ=255)
                aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                                                            >  2:96858/1‑151 (MQ=255)
                    acacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTc                                                                                        >  1:6173247/1‑151 (MQ=14)
                     cacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTca                                                                                       >  1:6991042/1‑151 (MQ=14)
                     cacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTca                                                                                       >  1:5466288/1‑151 (MQ=255)
                       cGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaac                                                                                     >  1:501951/1‑151 (MQ=14)
                        gAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaaca                                                                                    >  1:3923270/1‑151 (MQ=255)
                         aaTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                                                                   >  2:6359173/1‑151 (MQ=255)
                         aaTTGCTTGATTCGATTCGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCACGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                                                                   >  2:274430/1‑151 (MQ=17)
                          aTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCaacaac                                                                                  <  2:3608288/151‑1 (MQ=255)
                           ttGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACt                                                                                 >  1:2486267/1‑151 (MQ=255)
                            tGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACtt                                                                                <  1:6805406/151‑1 (MQ=255)
                            tGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACtt                                                                                >  2:1119052/1‑151 (MQ=17)
                            tGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACtt                                                                                <  2:5544372/151‑1 (MQ=255)
                            tGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACtt                                                                                >  2:902955/1‑151 (MQ=255)
                             gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTGTCTTTCGAAAGCTAACAACTTCAACAACTTa                                                                               >  2:5665558/1‑151 (MQ=17)
                              cTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAc                                                                              >  2:5029948/1‑151 (MQ=17)
                              cTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAc                                                                              >  1:535010/1‑151 (MQ=17)
                              cTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAc                                                                              <  1:6496470/151‑1 (MQ=17)
                              cTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAc                                                                              <  1:6812953/151‑1 (MQ=17)
                               ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                                                             >  2:6559759/1‑151 (MQ=17)
                                tGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCg                                                                            >  1:2668899/1‑151 (MQ=17)
                                   ttCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCtt                                                                         <  2:5217462/151‑1 (MQ=255)
                                   ttCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCtt                                                                         <  2:1245946/151‑1 (MQ=18)
                                     cGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAATTTCAACAACTTACCGCttag                                                                       >  2:6265727/1‑149 (MQ=18)
                                      gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                                                                      >  1:1920686/1‑151 (MQ=18)
                                       aTTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCTTCTCTATCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGccc                                                                     >  2:2802403/1‑151 (MQ=18)
                                       aTTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGccc                                                                     >  1:3112321/1‑151 (MQ=255)
                                           gTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTTTTTTTTTTTTCCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGaa                                                                 >  2:1371830/1‑151 (MQ=18)
                                            ttAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAg                                                                <  2:3496187/151‑1 (MQ=255)
                                            ttAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAg                                                                >  1:7174390/1‑151 (MQ=18)
                                               aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                                                             >  2:2472746/1‑151 (MQ=21)
                                               aaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGt                                                             >  1:4841742/1‑151 (MQ=255)
                                                 gagCGTTTGGGTAAGAGCTTTGCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTGTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTAACCGCTTGCCCCGAAGCGTTc                                                           >  2:363850/1‑151 (MQ=21)
                                                   ncGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCAt                                                         >  1:586412/2‑151 (MQ=21)
                                                    cGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATc                                                        >  2:4767561/1‑151 (MQ=21)
                                                        tGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTg                                                    <  1:6286428/151‑1 (MQ=255)
                                                         ggTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGc                                                   <  2:4683387/151‑1 (MQ=255)
                                                         ggTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGc                                                   <  1:2915638/151‑1 (MQ=255)
                                                          gTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCg                                                  >  2:3139191/1‑151 (MQ=25)
                                                          gTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCg                                                  <  1:3149044/151‑1 (MQ=255)
                                                           ttAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCga                                                 >  2:3878011/1‑151 (MQ=255)
                                                           ttAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCATCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCga                                                 <  2:603216/151‑1 (MQ=25)
                                                            tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag                                                >  1:6595862/1‑151 (MQ=255)
                                                            tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag                                                >  2:6721253/1‑151 (MQ=255)
                                                               gagCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgaggag                                             >  1:2960013/1‑151 (MQ=255)
                                                               gagCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgaggag                                             >  2:2314474/1‑151 (MQ=255)
                                                               gagCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgaggag                                             >  1:2959800/1‑151 (MQ=255)
                                                                agCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGaggagg                                            >  2:673609/1‑151 (MQ=255)
                                                                 gcttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGc                                           <  2:5372773/148‑1 (MQ=255)
                                                                        gTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaataat                                    >  2:3066558/1‑151 (MQ=255)
                                                                        gTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaataat                                    >  1:956329/1‑151 (MQ=255)
                                                                          ctcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAc                                  >  1:4455558/1‑151 (MQ=255)
                                                                           tcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcg                                 <  1:878419/151‑1 (MQ=255)
                                                                           tcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcg                                 >  2:3980450/1‑151 (MQ=255)
                                                                            cAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcgc                                <  2:1966617/151‑1 (MQ=255)
                                                                            cAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcgc                                <  2:1542130/151‑1 (MQ=255)
                                                                              aCCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACgcgc                              >  1:5702739/1‑151 (MQ=255)
                                                                               ccGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCt                             >  1:4656044/1‑151 (MQ=255)
                                                                               ccGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCGTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAACACGCGCt                             >  2:5589655/1‑151 (MQ=37)
                                                                                 gAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCttt                           <  1:1100417/151‑1 (MQ=255)
                                                                                 gAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCttt                           <  2:5305860/151‑1 (MQ=255)
                                                                                  aGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTg                          <  2:2713604/151‑1 (MQ=255)
                                                                                  aGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAAGCGCTTTg                          >  2:4837773/1‑151 (MQ=255)
                                                                                       cgcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATACTACGCGCTTTGAAATc                     >  2:6905665/1‑151 (MQ=255)
                                                                                       cgcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATc                     <  1:5317998/151‑1 (MQ=255)
                                                                                        gcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCa                    >  1:1581423/1‑151 (MQ=255)
                                                                                         cATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCag                   <  2:377732/151‑1 (MQ=255)
                                                                                          aTTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCaga                  <  1:4416434/151‑1 (MQ=255)
                                                                                           ttCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCagag                 >  1:5147222/1‑151 (MQ=255)
                                                                                             cTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTc               <  1:431903/151‑1 (MQ=255)
                                                                                                cGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAac            <  1:4757135/151‑1 (MQ=255)
                                                                                                cGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAac            >  2:428153/1‑151 (MQ=255)
                                                                                                cGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAac            <  2:1619488/151‑1 (MQ=255)
                                                                                                cGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAac            <  2:6376508/151‑1 (MQ=255)
                                                                                                 gCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAaca           >  2:7236526/1‑151 (MQ=255)
                                                                                                 gCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAaca           <  1:5547796/151‑1 (MQ=255)
                                                                                                  cTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAacac          >  2:7132685/1‑151 (MQ=255)
                                                                                                   tttCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACAcc         <  1:1753540/151‑1 (MQ=255)
                                                                                                   tttCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACAcc         <  1:3785814/151‑1 (MQ=255)
                                                                                                    ttCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCt        >  1:2207188/1‑151 (MQ=255)
                                                                                                      ccTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTg      >  2:4875507/1‑151 (MQ=255)
                                                                                                      ccTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTg      <  2:7025312/151‑1 (MQ=255)
                                                                                                       cTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGt     >  1:4044042/1‑151 (MQ=255)
                                                                                                       cTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGt     <  1:5679424/151‑1 (MQ=255)
                                                                                                       cTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGt     <  1:970068/151‑1 (MQ=255)
                                                                                                        tCAGAACCTGTCAAGCGTTTATTTTGTAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAGCTTCAACAACTTACCGCTTGCCCCGAAGCGTTGATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGtt    <  2:4698577/151‑1 (MQ=255)
                                                                                                          agaaCCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCt  >  1:3299091/1‑151 (MQ=255)
                                                                                                          agaaCCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCt  <  2:1046784/151‑1 (MQ=255)
                                                                                                          agaaCCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCt  <  1:5942392/151‑1 (MQ=255)
                                                                                                             |                                                                                                                                                   
GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCT  >  CP009974/775347‑775603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑