Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,154,311	T→C	T337T (ACT→ACC)	RPPX_05115 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,154,311	0	T	C	100.0%	43.6 / NA	23	T337T (ACT→ACC)	RPPX_05115	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/12);  total (11/12)

GTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACTGACCTGGCCCT  >  CP009974/1154164‑1154322                                                                                                                                                    |            gTTCAGCATTACCCTGCGACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACcgaccgac          <  2:1149679/151‑1 (MQ=25) gTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACcgaccgac          >  2:7078766/1‑151 (MQ=25) gTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACcgaccgac          >  2:7078765/1‑151 (MQ=25) gTTCAGCAGTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACcgaccgac          <  2:166764/151‑1 (MQ=14)  ttCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCgaccgacc         <  1:6027202/151‑1 (MQ=25)   tccgCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcct        <  2:3558727/148‑1 (MQ=14)   tCAGGATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcct        <  1:4453483/151‑1 (MQ=17)   tCAGCATTCCCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcct        <  1:1997024/151‑1 (MQ=18)   tCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCgaccgaccc        >  2:5022826/1‑150 (MQ=21)   tCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcct        <  2:5719428/151‑1 (MQ=18)   tCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcct        >  2:3020796/1‑151 (MQ=21)    cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       <  1:4475679/151‑1 (MQ=18)    cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       <  2:5931467/151‑1 (MQ=18)    cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       <  1:1819863/151‑1 (MQ=18)     aGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGTTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctgg      >  1:720337/1‑151 (MQ=17)     aGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctgg      >  2:2505742/1‑151 (MQ=17)     aGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctgg      >  1:2587629/1‑151 (MQ=17)      gCATTGCCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGTCAATGGCTCGCCAGCCGCCAACATCACGGCACCGGACGTCGACGCCGCTGTTCCGACAGCCCCTGAGCAGCCGACCGAcctggc     <  2:7206032/151‑1 (MQ=11)      gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATGGCTCGCCAGTTGCCAACATGGCGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     <  2:5211603/151‑1 (MQ=255)      gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     >  2:4174286/1‑151 (MQ=14)      gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     <  2:2073582/151‑1 (MQ=14)      gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     <  1:306098/151‑1 (MQ=14)      gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGGCAGCGCCGCAGATGCGGCAGGCAATAGCGCGCCAGTCGCCAACATCACGGCACCGGCCGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctgtc     >  2:4834187/1‑149 (MQ=11)       cATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGCCCGCcctcgcc    >  2:3917632/1‑151 (MQ=9)       cATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggcc    >  2:2485075/1‑151 (MQ=14)       cATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggcc    >  1:4691903/1‑151 (MQ=14)        aTTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccc   >  1:6880745/1‑151 (MQ=14)        aTTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccc   >  2:618695/1‑151 (MQ=14)         ttACCCTGCACCCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGAGGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccct  <  2:4442355/151‑1 (MQ=2)                                                                                                                                                    |            GTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACTGACCTGGCCCT  >  CP009974/1154164‑1154322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑